KEGG   ORTHOLOGY: K21673
Entry
K21673            Tight     KO                                     

Name
cdhA
Definition
caffeine dehydrogenase subunit alpha [EC:1.17.5.2]
Pathway
ko00232  Caffeine metabolism
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00232 Caffeine metabolism
    K21673  cdhA; caffeine dehydrogenase subunit alpha
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.17  Acting on CH or CH2 groups
   1.17.5  With a quinone or similar compound as acceptor
    1.17.5.2  caffeine dehydrogenase
     K21673  cdhA; caffeine dehydrogenase subunit alpha
Other DBs
RN: R07980
COG: COG1529
Genes
AG: ADH15879(cdhA)
Reference
  Authors
Yu CL, Kale Y, Gopishetty S, Louie TM, Subramanian M
  Title
A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid.
  Journal
J Bacteriol 190:772-6 (2008)
DOI:10.1128/JB.01390-07
  Sequence

KEGG   ORTHOLOGY: K21674
Entry
K21674            Tight     KO                                     

Name
cdhB
Definition
caffeine dehydrogenase subunit beta [EC:1.17.5.2]
Pathway
ko00232  Caffeine metabolism
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00232 Caffeine metabolism
    K21674  cdhB; caffeine dehydrogenase subunit beta
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.17  Acting on CH or CH2 groups
   1.17.5  With a quinone or similar compound as acceptor
    1.17.5.2  caffeine dehydrogenase
     K21674  cdhB; caffeine dehydrogenase subunit beta
Other DBs
RN: R07980
Genes
AG: ADH15880(cdhB)
Reference
  Authors
Yu CL, Kale Y, Gopishetty S, Louie TM, Subramanian M
  Title
A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid.
  Journal
J Bacteriol 190:772-6 (2008)
DOI:10.1128/JB.01390-07
  Sequence

KEGG   ORTHOLOGY: K21675
Entry
K21675            Tight     KO                                     

Name
cdhC
Definition
caffeine dehydrogenase subunit gamma [EC:1.17.5.2]
Pathway
ko00232  Caffeine metabolism
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00232 Caffeine metabolism
    K21675  cdhC; caffeine dehydrogenase subunit gamma
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.17  Acting on CH or CH2 groups
   1.17.5  With a quinone or similar compound as acceptor
    1.17.5.2  caffeine dehydrogenase
     K21675  cdhC; caffeine dehydrogenase subunit gamma
Other DBs
RN: R07980
COG: COG2080
Genes
AG: ADH15881(cdhC)
Reference
  Authors
Yu CL, Kale Y, Gopishetty S, Louie TM, Subramanian M
  Title
A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid.
  Journal
J Bacteriol 190:772-6 (2008)
DOI:10.1128/JB.01390-07
  Sequence

KEGG   ENZYME: 1.17.5.2
Entry
EC 1.17.5.2                 Enzyme                                 

Name
caffeine dehydrogenase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With a quinone or similar compound as acceptor
Sysname
caffeine:ubiquinone oxidoreductase
Reaction(IUBMB)
caffeine + ubiquinone + H2O = 1,3,7-trimethylurate + ubiquinol [RN:R07980]
Reaction(KEGG)
R07980
Substrate
caffeine [CPD:C07481];
ubiquinone [CPD:C00399];
H2O [CPD:C00001]
Product
1,3,7-trimethylurate [CPD:C16361];
ubiquinol [CPD:C00390]
Comment
This enzyme, characterized from the soil bacterium Pseudomonas sp. CBB1, catalyses the incorporation of an oxygen atom originating from a water molecule into position C-8 of caffeine. The enzyme utilizes short-tail ubiquinones as the preferred electron acceptor.
History
EC 1.17.5.2 created 2010
Pathway
ec00232  Caffeine metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K21673  caffeine dehydrogenase subunit alpha
K21674  caffeine dehydrogenase subunit beta
K21675  caffeine dehydrogenase subunit gamma
Reference
1  [PMID:17981969]
  Authors
Yu CL, Kale Y, Gopishetty S, Louie TM, Subramanian M
  Title
A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid.
  Journal
J Bacteriol 190:772-6 (2008)
DOI:10.1128/JB.01390-07
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.5.2
IUBMB Enzyme Nomenclature: 1.17.5.2
ExPASy - ENZYME nomenclature database: 1.17.5.2
BRENDA, the Enzyme Database: 1.17.5.2

KEGG   REACTION: R07980
Entry
R07980                      Reaction                               

Name
caffeine:ubiquinone oxidoreductase
Definition
Caffeine + Ubiquinone + H2O <=> 1,3,7-Trimethyluric acid + Ubiquinol
Equation
Reaction class
RC00061  C00390_C00399
RC02017  C07481_C16361
Enzyme
Pathway
rn00232  Caffeine metabolism
rn01120  Microbial metabolism in diverse environments
Orthology
K21673  caffeine dehydrogenase subunit alpha [EC:1.17.5.2]
K21674  caffeine dehydrogenase subunit beta [EC:1.17.5.2]
K21675  caffeine dehydrogenase subunit gamma [EC:1.17.5.2]
Other DBs
RHEA: 47151

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