KEGG   ORTHOLOGY: K21925
Entry
K21925            Tight     KO                                     

Name
TPS3
Definition
(-)-camphene/tricyclene/(4S)-limonene/myrcene synthase [EC:4.2.3.117 4.2.3.105 4.2.3.16 4.2.3.15]
Pathway
ko00902  Monoterpenoid biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00902 Monoterpenoid biosynthesis
    K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.15  myrcene synthase
     K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
    4.2.3.16  (4S)-limonene synthase
     K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
    4.2.3.105  tricyclene synthase
     K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
    4.2.3.117  (-)-camphene synthase
     K21925  TPS3; (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
Other DBs
RN: R02009 R02013 R09972
GO: 0102703 0102701 0050552 0050551
Genes
SLY: 101245212(TPS3)
SPEN: 107012939
SOT: 102591915
Reference
  Authors
Falara V, Akhtar TA, Nguyen TT, Spyropoulou EA, Bleeker PM, Schauvinhold I, Matsuba Y, Bonini ME, Schilmiller AL, Last RL, Schuurink RC, Pichersky E
  Title
The tomato terpene synthase gene family.
  Journal
Plant Physiol 157:770-89 (2011)
DOI:10.1104/pp.111.179648
  Sequence
[sly:101245212]

KEGG   ORTHOLOGY: K22208
Entry
K22208            Tight     KO                                     

Name
AG6
Definition
(-)-camphene synthase [EC:4.2.3.117]
Pathway
ko00902  Monoterpenoid biosynthesis
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00902 Monoterpenoid biosynthesis
    K22208  AG6; (-)-camphene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.117  (-)-camphene synthase
     K22208  AG6; (-)-camphene synthase
Other DBs
RN: R09972
GO: 0102703
Genes
AG: AAB70707(AG6)
Reference
  Authors
Bohlmann J, Phillips M, Ramachandiran V, Katoh S, Croteau R
  Title
cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis).
  Journal
Arch Biochem Biophys 368:232-43 (1999)
DOI:10.1006/abbi.1999.1332
  Sequence

KEGG   ENZYME: 4.2.3.117
Entry
EC 4.2.3.117                Enzyme                                 

Name
(-)-camphene synthase;
CS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
geranyl-diphosphate diphosphate-lyase [cyclizing, (-)-camphene-forming]
Reaction(IUBMB)
geranyl diphosphate = (-)-camphene + diphosphate [RN:R09972]
Reaction(KEGG)
R09972
Substrate
geranyl diphosphate [CPD:C00341]
Product
(-)-camphene [CPD:C06305];
diphosphate [CPD:C00013]
Comment
(-)-Camphene is the major product in Abies grandis (grand fir) with traces of other monoterpenoids [1]. In Pseudotsuga menziesii (Douglas-fir) there are about equal parts of (-)-camphene and (-)-alpha-pinene with traces of four other monoterpenoids [2,3]. In Solanum lycopersicum (tomato) tricyclene, beta-myrcene, limonene, and traces of several other monoterpenoids are also formed [4]. See also EC 4.2.3.15 myrcene synthase, EC 4.2.3.16 (4S)-limonene synthase, EC 4.2.3.119 (-)-alpha-pinene synthase and EC 4.2.3.105 tricyclene synthase.
History
EC 4.2.3.117 created 2012
Pathway
ec00902  Monoterpenoid biosynthesis
ec01100  Metabolic pathways
Orthology
K21925  (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
K22208  (-)-camphene synthase
Genes
SLY: 101245212(TPS3)
SPEN: 107012939
SOT: 102591915
Reference
1  [PMID:10441373]
  Authors
Bohlmann J, Phillips M, Ramachandiran V, Katoh S, Croteau R
  Title
cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis).
  Journal
Arch Biochem Biophys 368:232-43 (1999)
DOI:10.1006/abbi.1999.1332
  Sequence
Reference
2  [PMID:15921711]
  Authors
Huber DP, Philippe RN, Godard KA, Sturrock RN, Bohlmann J
  Title
Characterization of four terpene synthase cDNAs from methyl jasmonate-induced Douglas-fir, Pseudotsuga menziesii.
  Journal
Phytochemistry 66:1427-39 (2005)
DOI:10.1016/j.phytochem.2005.04.030
Reference
3  [PMID:15978541]
  Authors
Hyatt DC, Croteau R
  Title
Mutational analysis of a monoterpene synthase reaction: altered catalysis through directed mutagenesis of (-)-pinene synthase from Abies grandis.
  Journal
Arch Biochem Biophys 439:222-33 (2005)
DOI:10.1016/j.abb.2005.05.017
Reference
4  [PMID:21813655]
  Authors
Falara V, Akhtar TA, Nguyen TT, Spyropoulou EA, Bleeker PM, Schauvinhold I, Matsuba Y, Bonini ME, Schilmiller AL, Last RL, Schuurink RC, Pichersky E
  Title
The tomato terpene synthase gene family.
  Journal
Plant Physiol 157:770-89 (2011)
DOI:10.1104/pp.111.179648
  Sequence
[sly:101245212]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.117
IUBMB Enzyme Nomenclature: 4.2.3.117
ExPASy - ENZYME nomenclature database: 4.2.3.117
BRENDA, the Enzyme Database: 4.2.3.117

KEGG   REACTION: R09972
Entry
R09972                      Reaction                               

Name
geranyl-diphosphate diphosphate-lyase [cyclizing, (-)-camphene-forming]
Definition
Geranyl diphosphate <=> (-)-Camphene + Diphosphate
Equation
Reaction class
RC02304  C00341_C06305
Enzyme
Pathway
rn00902  Monoterpenoid biosynthesis
rn01100  Metabolic pathways
Orthology
K21925  (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase [EC:4.2.3.117 4.2.3.105 4.2.3.16 4.2.3.15]
K22208  (-)-camphene synthase [EC:4.2.3.117]
Other DBs
RHEA: 25487

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