KEGG   ORTHOLOGY: K22113
Entry
K22113                      KO                                     
Symbol
ddaG
Name
fumarate---(S)-2,3-diaminopropanoate ligase [EC:6.3.2.46]
Pathway
map00998  Biosynthesis of various antibiotics
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00904  Dapdiamides biosynthesis, L-2,3-diaminopropanoate => dapdiamide A/B/C
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various antibiotics
    K22113  ddaG; fumarate---(S)-2,3-diaminopropanoate ligase
Enzymes [BR:ko01000]
 6. Ligases
  6.3  Forming carbon-nitrogen bonds
   6.3.2  Acid-D-amino-acid ligases (peptide synthases)
    6.3.2.46  fumarate---(S)-2,3-diaminopropanoate ligase
     K22113  ddaG; fumarate---(S)-2,3-diaminopropanoate ligase
Other DBs
RN: R10940
Genes
SPE: Spro_0345
SPLY: Q5A_016900
PVA: Pvag_pPag20164
PAGC: BEE12_22715
PMAM: KSS90_08440
PALO: E6C60_3194
PCEL: HUB94_19670
PALR: HGI30_17415
CCHL: FPL14_15945
SGD: ELQ87_36080
AG: ADN39487(ddaG)
 » show all
Reference
  Authors
Hollenhorst MA, Clardy J, Walsh CT
  Title
The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics.
  Journal
Biochemistry 48:10467-72 (2009)
DOI:10.1021/bi9013165
  Sequence

KEGG   ENZYME: 6.3.2.46
Entry
EC 6.3.2.46                 Enzyme                                 
Name
fumarate---(S)-2,3-diaminopropanoate ligase;
DdaG;
fumarate:(S)-2,3-diaminopropanoate ligase (AMP-forming)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
fumarate:L-2,3-diaminopropanoate ligase (AMP-forming)
Reaction(IUBMB)
ATP + fumarate + L-2,3-diaminopropanoate = AMP + diphosphate + N3-fumaroyl-L-2,3-diaminopropanoate [RN:R10940]
Reaction(KEGG)
R10940
Substrate
ATP [CPD:C00002];
fumarate [CPD:C00122];
L-2,3-diaminopropanoate [CPD:C03401]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
N3-fumaroyl-L-2,3-diaminopropanoate [CPD:C20961]
Comment
The enzyme, characterized from the bacterium Enterobacter agglomerans, is involved in biosynthesis of dapdiamide tripeptide antibiotics, a family of fumaramoyl- and epoxysuccinamoyl-peptides named for the presence of an L-2,3-diaminopropanoate (DAP) moiety and two amide linkages in their scaffold.
History
EC 6.3.2.46 created 2015
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22113  fumarate---(S)-2,3-diaminopropanoate ligase
Genes
SPESpro_0345
SPLYQ5A_016900
PVAPvag_pPag20164
PAGCBEE12_22715
PMAMKSS90_08440
PALOE6C60_3194
PCELHUB94_19670
PALRHGI30_17415
CCHLFPL14_15945
SGDELQ87_36080
SAOVG3H79_05910
 » show all
Reference
1  [PMID:19807062]
  Authors
Hollenhorst MA, Clardy J, Walsh CT
  Title
The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics.
  Journal
Biochemistry 48:10467-72 (2009)
DOI:10.1021/bi9013165
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.46
IUBMB Enzyme Nomenclature: 6.3.2.46
ExPASy - ENZYME nomenclature database: 6.3.2.46
BRENDA, the Enzyme Database: 6.3.2.46

KEGG   REACTION: R10940
Entry
R10940                      Reaction                               
Name
fumarate:L-2,3-diaminopropanoate ligase (AMP-forming)
Definition
ATP + Fumarate + L-2,3-Diaminopropanoate <=> AMP + Diphosphate + N3-Fumaroyl-L-2,3-diaminopropanoate
Equation
Reaction class
RC00096  C03401_C20961
RC00957  C00122_C20961
Enzyme
Pathway
rn00998  Biosynthesis of various antibiotics
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00904  Dapdiamides biosynthesis, L-2,3-diaminopropanoate => dapdiamide A/B/C
Orthology
K22113  fumarate---(S)-2,3-diaminopropanoate ligase [EC:6.3.2.46]
Other DBs
RHEA: 44343

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