KEGG   ORTHOLOGY: K22434
Entry
K22434            Tight     KO                                     
Symbol
PSIH
Name
tryptamine 4-monooxygenase [EC:1.14.99.59]
Pathway
map00901  Indole alkaloid biosynthesis
map01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00901 Indole alkaloid biosynthesis
    K22434  PSIH; tryptamine 4-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.99  Miscellaneous
    1.14.99.59  tryptamine 4-monooxygenase
     K22434  PSIH; tryptamine 4-monooxygenase
Other DBs
RN: R11912
COG: COG2124
Genes
PCUB: JR316_0010786
AG: ASU62246(psiH)
Reference
  Authors
Fricke J, Blei F, Hoffmeister D
  Title
Enzymatic Synthesis of Psilocybin.
  Journal
Angew Chem Int Ed Engl 56:12352-12355 (2017)
DOI:10.1002/anie.201705489
  Sequence

KEGG   ENZYME: 1.14.99.59
Entry
EC 1.14.99.59               Enzyme                                 
Name
tryptamine 4-monooxygenase;
PsiH
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
tryptamine,hydrogen-donor:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
tryptamine + reduced acceptor + O2 = 4-hydroxytryptamine + acceptor + H2O [RN:R11912]
Reaction(KEGG)
R11912
Substrate
tryptamine [CPD:C00398];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
4-hydroxytryptamine [CPD:C21762];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the fungus Psilocybe cubensis. Involved in the biosynthesis of the psychoactive compound psilocybin.
History
EC 1.14.99.59 created 2017
Pathway
ec00901  Indole alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K22434  tryptamine 4-monooxygenase
Genes
PCUBJR316_0010786
Reference
1  [PMID:28763571]
  Authors
Fricke J, Blei F, Hoffmeister D
  Title
Enzymatic Synthesis of Psilocybin.
  Journal
Angew Chem Int Ed Engl 56:12352-12355 (2017)
DOI:10.1002/anie.201705489
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.59
IUBMB Enzyme Nomenclature: 1.14.99.59
ExPASy - ENZYME nomenclature database: 1.14.99.59
BRENDA, the Enzyme Database: 1.14.99.59

KEGG   REACTION: R11912
Entry
R11912                      Reaction                               
Name
tryptamine,hydrogen-donor:oxygen oxidoreductase (4-hydroxylating)
Definition
Tryptamine + Reduced acceptor + Oxygen <=> 4-Hydroxytryptamine + Acceptor + H2O
Equation
Reaction class
RC00046  C00398_C21762
Enzyme
Pathway
rn00901  Indole alkaloid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K22434  tryptamine 4-monooxygenase [EC:1.14.99.59]
Other DBs
RHEA: 15868

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