KEGG   ORTHOLOGY: K23136
Entry
K23136                      KO                                     
Symbol
CYP82C4
Name
fraxetin 5-hydroxylase [EC:1.14.14.164]
Pathway
map00999  Biosynthesis of various plant secondary metabolites
map01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various plant secondary metabolites
    K23136  CYP82C4; fraxetin 5-hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K23136  CYP82C4; fraxetin 5-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.164  fraxetin 5-hydroxylase
     K23136  CYP82C4; fraxetin 5-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP82 family
   K23136  CYP82C4; fraxetin 5-hydroxylase
Other DBs
RN: R12223
GO: 0106144
Genes
ATH: AT4G31940(CYP82C4)
ALY: 9303340
CRB: 17879967
CSAT: 104716994 104720023 104721639 104727626 104730082 104733184
BRP: 103835488
BNA: 106390013 106449600
BOE: 106333001
RSZ: 108821793
THJ: 104803692 104819460 104819461
CPAP: 110813494
CIT: 102608597
CIC: CICLE_v10003803mg
PVY: 116137768 116140031
MINC: 123228524
TCC: 18591293
GRA: 105769156
GHI: 107888141 107942681
GAB: 108471489
DZI: 111311379 111314823
GMX: 100811993
GSJ: 114408673
PVU: PHAVU_009G061600g
VRA: 106760470
VAR: 108342698
VUN: 114162602
CCAJ: 109802833
APRC: 113865890
LJA: Lj1g3v1615300.1(Lj1g3v1615300.1)
ADU: 107460456
AIP: 107610187
AHF: 112705794 112762412
LANG: 109343928
FVE: 105350002
PPER: 18789706
PMUM: 103320922
PAVI: 110762562
PDUL: 117615155
MDM: 103441505
PXB: 103954079
MNT: 112091840 21404129
RCU: 8260896 8260897 8260900
JCU: 105633709 105633710 105633711
HBR: 110661464 110661466
MESC: 110614589 110615124 110616234
POP: 18104964
PEU: 105130198
PALZ: 118034182 118034206 118036573
JRE: 109010345
CILL: 122297470
QSU: 112004233
QLO: 115957904
TWL: 120000202
VVI: 100247206
VRI: 117907345
SLY: 101267593
SPEN: 107007174
SOT: 102581162
SSTN: 125861990
NTA: 107782393 107790829
NSY: 104246870
NTO: 104094857
NAU: 109215771
INI: 109169781
ITR: 116013393
OEU: 111381794
HAN: 110868438 110914130
ECAD: 122609547
LSV: 111876237 111876238 111893788
CCAV: 112511847
CSIN: 114289226 114302463 114311935 114311955
NNU: 104594945 104602643
MING: 122057355 122057456 122062028 122064336 122064730 122064731 122066479 122069348 122070247 122072865 122072912 122073149 122073602 122074394 122074395 122074740 122074750 122075296 122075812 122075964 122076219 122076251 122077292 122079511 122086196 122086257 122093030
TSS: 122638622 122643304 122643712 122645181 122645182 122645310 122645792 122645793 122645796 122645799 122645805 122646692 122648499 122648500 122648503 122650927 122654093 122654104 122654105 122655608 122665079 122665816 122669424 122669804 122669805 122669806 122672531 122672552
NCOL: 116245832 116245835 116245851 116245854 116245899 116245912 116248524 116249848 116249858 116249984 116249996 116250038 116250385 116252370 116252483 116253042 116253192 116253742
 » show all
Reference
PMID:29581584
  Authors
Rajniak J, Giehl RFH, Chang E, Murgia I, von Wiren N, Sattely ES
  Title
Biosynthesis of redox-active metabolites in response to iron deficiency in plants.
  Journal
Nat Chem Biol 14:442-450 (2018)
DOI:10.1038/s41589-018-0019-2
  Sequence
[ath:AT4G31940]

  All links  
Ontology (3)   
   KEGG BRITE (3)   
Pathway (4)   
   KEGG PATHWAY (4)   
Chemical reaction (3)   
   KEGG ENZYME (1)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (193)   
   KEGG GENES (174)   
   OC (19)   
All databases (203)   

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KEGG   ENZYME: 1.14.14.164
Entry
EC 1.14.14.164              Enzyme                                 
Name
fraxetin 5-hydroxylase;
CYP82C4;
fraxetin 5-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
fraxetin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
fraxetin + [reduced NADPH---hemoprotein reductase] + O2 = sideretin (reduced form) + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12223]
Reaction(KEGG)
R12223
Substrate
fraxetin [CPD:C09265];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
sideretin [CPD:C22007];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein involved in biosynthesis of iron(III)-chelating coumarins in higher plants.
History
EC 1.14.14.164 created 2018
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01110  Biosynthesis of secondary metabolites
Orthology
K23136  fraxetin 5-hydroxylase
Genes
ATH: AT4G31940(CYP82C4)
ALY: 9303340
CRB: 17879967
CSAT: 104716994 104720023 104721639 104727626 104730082 104733184
BRP: 103835488
BNA: 106390013 106449600
BOE: 106333001
RSZ: 108821793
THJ: 104803692 104819460 104819461
CPAP: 110813494
CIT: 102608597
CIC: CICLE_v10003803mg
PVY: 116137768 116140031
MINC: 123228524
TCC: 18591293
GRA: 105769156
GHI: 107888141 107942681
GAB: 108471489
DZI: 111311379 111314823
GMX: 100811993
GSJ: 114408673
PVU: PHAVU_009G061600g
VRA: 106760470
VAR: 108342698
VUN: 114162602
CCAJ: 109802833
APRC: 113865890
LJA: Lj1g3v1615300.1(Lj1g3v1615300.1)
ADU: 107460456
AIP: 107610187
AHF: 112705794 112762412
LANG: 109343928
FVE: 105350002
PPER: 18789706
PMUM: 103320922
PAVI: 110762562
PDUL: 117615155
MDM: 103441505
PXB: 103954079
MNT: 112091840 21404129
RCU: 8260896 8260897 8260900
JCU: 105633709 105633710 105633711
HBR: 110661464 110661466
MESC: 110614589 110615124 110616234
POP: 18104964
PEU: 105130198
PALZ: 118034182 118034206 118036573
JRE: 109010345
CILL: 122297470
QSU: 112004233
QLO: 115957904
TWL: 120000202
VVI: 100247206
VRI: 117907345
SLY: 101267593
SPEN: 107007174
SOT: 102581162
SSTN: 125861990
NTA: 107782393 107790829
NSY: 104246870
NTO: 104094857
NAU: 109215771
INI: 109169781
ITR: 116013393
OEU: 111381794
HAN: 110868438 110914130
ECAD: 122609547
LSV: 111876237 111876238 111893788
CCAV: 112511847
CSIN: 114289226 114302463 114311935 114311955
NNU: 104594945 104602643
MING: 122057355 122057456 122062028 122064336 122064730 122064731 122066479 122069348 122070247 122072865 122072912 122073149 122073602 122074394 122074395 122074740 122074750 122075296 122075812 122075964 122076219 122076251 122077292 122079511 122086196 122086257 122093030
TSS: 122638622 122643304 122643712 122645181 122645182 122645310 122645792 122645793 122645796 122645799 122645805 122646692 122648499 122648500 122648503 122650927 122654093 122654104 122654105 122655608 122665079 122665816 122669424 122669804 122669805 122669806 122672531 122672552
NCOL: 116245832 116245835 116245851 116245854 116245899 116245912 116248524 116249848 116249858 116249984 116249996 116250038 116250385 116252370 116252483 116253042 116253192 116253742
 » show all
Reference
1  [PMID:29581584]
  Authors
Rajniak J, Giehl RFH, Chang E, Murgia I, von Wiren N, Sattely ES
  Title
Biosynthesis of redox-active metabolites in response to iron deficiency in plants.
  Journal
Nat Chem Biol 14:442-450 (2018)
DOI:10.1038/s41589-018-0019-2
  Sequence
[ath:AT4G31940]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.164
IUBMB Enzyme Nomenclature: 1.14.14.164
ExPASy - ENZYME nomenclature database: 1.14.14.164
BRENDA, the Enzyme Database: 1.14.14.164

  All links  
Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (6)   
   KEGG COMPOUND (6)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (175)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (174)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (4)   
   InterPro (4)   
All databases (193)   

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KEGG   REACTION: R12223
Entry
R12223                      Reaction                               
Name
fraxetin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5-hydroxylating)
Definition
Fraxetin + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Sideretin + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
C09265 + C03024 + C00007 <=> C22007 + C03161 + C00001
Reaction class
RC00236  C09265_C22007
Enzyme
1.14.14.164
Pathway
rn00999  Biosynthesis of various plant secondary metabolites
rn01110  Biosynthesis of secondary metabolites
Orthology
K23136  fraxetin 5-hydroxylase [EC:1.14.14.164]

  All links  
Ontology (1)   
   KEGG BRITE (1)   
Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (6)   
   KEGG COMPOUND (6)   
Chemical reaction (2)   
   KEGG ENZYME (1)   
   KEGG RCLASS (1)   
Gene (175)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (174)   
All databases (188)   

Download RDF
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