KEGG   ORTHOLOGY: K23157
Entry
K23157            Tight     KO                                     
Symbol
OMT2
Name
3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase subunit OMT2 [EC:2.1.1.352]
Pathway
map00950  Isoquinoline alkaloid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00946  Noscapine biosynthesis, (S)-reticuline => noscapine
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00950 Isoquinoline alkaloid biosynthesis
    K23157  OMT2; 3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase subunit OMT2
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.352  3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase
     K23157  OMT2; 3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase subunit OMT2
Other DBs
RN: R11692 R12262
Genes
PSOM: 113322802
Reference
  Authors
Park MR, Chen X, Lang DE, Ng KKS, Facchini PJ
  Title
Heterodimeric O-methyltransferases involved in the biosynthesis of noscapine in opium poppy.
  Journal
Plant J 95:252-267 (2018)
DOI:10.1111/tpj.13947
  Sequence
[psom:113322802]

KEGG   ORTHOLOGY: K23158
Entry
K23158            Tight     KO                                     
Symbol
OMT3
Name
3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase subunit OMT3
Pathway
map00950  Isoquinoline alkaloid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00946  Noscapine biosynthesis, (S)-reticuline => noscapine
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00950 Isoquinoline alkaloid biosynthesis
    K23158  OMT3; 3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase subunit OMT3
Other DBs
RN: R11692 R12262
Genes
PSOM: 113322330
Reference
  Authors
Park MR, Chen X, Lang DE, Ng KKS, Facchini PJ
  Title
Heterodimeric O-methyltransferases involved in the biosynthesis of noscapine in opium poppy.
  Journal
Plant J 95:252-267 (2018)
DOI:10.1111/tpj.13947
  Sequence
[psom:113322330]

KEGG   ENZYME: 2.1.1.352
Entry
EC 2.1.1.352                Enzyme                                 
Name
3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 3-O-acetyl-4'-O-demethylpapaveroxine = S-adenosyl-L-homocysteine + 3-O-acetylpapaveroxine [RN:R11692]
Reaction(KEGG)
R11692;
(other) R12262
Substrate
S-adenosyl-L-methionine [CPD:C00019];
3-O-acetyl-4'-O-demethylpapaveroxine [CPD:C21590]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
3-O-acetylpapaveroxine [CPD:C21591]
Comment
This activity is part of the noscapine biosynthesis pathway, as characterized in the plant Papaver somniferum (opium poppy). It is catalysed by heterodimeric complexes of the OMT2 gene product and the product of either OMT3 or 6OMT. OMT2 is the catalytic subunit in both complexes.
History
EC 2.1.1.352 created 2018
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K23157  3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase subunit OMT2
Genes
PSOM113322802
Reference
1  [PMID:27378283]
  Authors
Li Y, Smolke CD
  Title
Engineering biosynthesis of the anticancer alkaloid noscapine in yeast.
  Journal
Nat Commun 7:12137 (2016)
DOI:10.1038/ncomms12137
Reference
2  [PMID:29723437]
  Authors
Park MR, Chen X, Lang DE, Ng KKS, Facchini PJ
  Title
Heterodimeric O-methyltransferases involved in the biosynthesis of noscapine in opium poppy.
  Journal
Plant J 95:252-267 (2018)
DOI:10.1111/tpj.13947
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.352
IUBMB Enzyme Nomenclature: 2.1.1.352
ExPASy - ENZYME nomenclature database: 2.1.1.352
BRENDA, the Enzyme Database: 2.1.1.352

KEGG   REACTION: R11692
Entry
R11692                      Reaction                               
Name
S-adenosyl-L-methionine:3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase
Definition
4'-O-Desmethyl-3-O-acetylpapaveroxine + S-Adenosyl-L-methionine <=> 3-O-Acetylpapaveroxine + S-Adenosyl-L-homocysteine
Equation
Comment
O-methyltransferase
Reaction class
RC00392  C21590_C21591
Enzyme
Pathway
rn00950  Isoquinoline alkaloid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00946  Noscapine biosynthesis, (S)-reticuline => noscapine
Orthology
K23157  3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase subunit OMT2 [EC:2.1.1.352]
K23158  3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase subunit OMT3
Other DBs
RHEA: 57399

DBGET integrated database retrieval system