KEGG   ORTHOLOGY: K23375
Entry
K23375                      KO                                     
Symbol
sbnC
Name
staphyloferrin B synthase [EC:6.3.2.56]
Pathway
map00997  Biosynthesis of various other secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00875  Staphyloferrin B biosynthesis, L-serine => staphyloferrin B
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00997 Biosynthesis of various other secondary metabolites
    K23375  sbnC; staphyloferrin B synthase
Enzymes [BR:ko01000]
 6. Ligases
  6.3  Forming carbon-nitrogen bonds
   6.3.2  Acid-D-amino-acid ligases (peptide synthases)
    6.3.2.56  staphyloferrin B synthase
     K23375  sbnC; staphyloferrin B synthase
Other DBs
RN: R12312
COG: COG4264
Genes
DDD: Dda3937_02849(acsA)
DZE: Dd1591_2672
DDC: Dd586_2579
DZC: W909_07430
DSO: A4U42_16825
CED: LH89_04350
DFN: CVE23_08430
DDQ: DDI_1279
DAQ: DAQ1742_01516(acsA)
DIC: Dpoa569_002353
SGL: SGP1_0039
XDO: XDD1_1968
VTA: A3468
PSB: Psyr_2589
PSYR: N018_14365
PSEM: TO66_16010
PSIL: PMA3_09145
PALI: A3K91_1227
ABY: ABAYE2004
ABN: AB57_1873
ABB: ABBFA_01824(iucC_1)
ABX: ABK1_2134
ABH: M3Q_2028
ABAD: ABD1_16360
ABAZ: P795_9030
ABAU: IX87_19075
ABAA: IX88_10160
AGU: AS4_16150
SDN: Sden_0588
HEL: HELO_3332
RSO: RSp0419
RSE: F504_3905
RSY: RSUY_34870(iucC_1)
RME: Rmet_1115
MNO: Mnod_6950
MAQU: Maq22A_c25435(rhbC)
PCAL: BV455_03922(iucC_2)
SAU: SA0114(sbnC)
SAV: SAV0118
SAW: SAHV_0117
SAM: MW0091(sbnC)
SAS: SAS0092
SAR: SAR0121
SAC: SACOL0102
SAA: SAUSA300_0120(sbnC)
SAE: NWMN_0062(sbnC)
SAD: SAAV_0086
SUU: M013TW_0108(sbnC)
SUE: SAOV_0065
SUJ: SAA6159_00101(sbnC)
SUK: SAA6008_00096(sbnC)
SUC: ECTR2_74
SUZ: MS7_0110
SUW: SATW20_01300(sbnC)
SUG: SAPIG0129
SUF: SARLGA251_00930(sbnC)
SAUA: SAAG_00602
SAUE: RSAU_000072(sbnC)
SAUS: SA40_0085(sbnC)
SAUU: SA957_0100(sbnC)
SAUG: SA268_0097(sbnC)
SAUZ: SAZ172_0129(sbnC)
SAUF: X998_0097
SAB: SAB0057
SUY: SA2981_0119(sbnC)
SAUB: C248_0106
SAUM: BN843_1200
SAUC: CA347_129
SAUR: SABB_01722(sbnC)
SAUI: AZ30_00615
SAUD: CH52_05130
SAMS: NI36_00510
SDT: SPSE_2122
SDP: NCTC12225_02339(iucC_4)
SSCH: LH95_00365
SSCZ: RN70_00585
SAGQ: EP23_09760(sbnC)
SARL: SAP2_02050
SSH: NCTC13712_00071(iucC_1)
SRAI: LN051_00240(sbnC)
SFF: FOB90_07425(sbnC)
PPOL: X809_04505
SALJ: SMD11_0168
 » show all
Reference
  Authors
Dale SE, Doherty-Kirby A, Lajoie G, Heinrichs DE
  Title
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
  Journal
Infect Immun 72:29-37 (2004)
DOI:10.1128/IAI.72.1.29-37.2004
  Sequence
[sam:MW0091]
Reference
  Authors
Cheung J, Beasley FC, Liu S, Lajoie GA, Heinrichs DE
  Title
Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus.
  Journal
Mol Microbiol 74:594-608 (2009)
DOI:10.1111/j.1365-2958.2009.06880.x

KEGG   ENZYME: 6.3.2.56
Entry
EC 6.3.2.56                 Enzyme                                 
Name
staphyloferrin B synthase;
sbnC (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate:2-oxoglutarate ligase (staphyloferrin B-forming)
Reaction(IUBMB)
ATP + 2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate + 2-oxoglutarate = AMP + diphosphate + staphyloferrin B [RN:R12312]
Reaction(KEGG)
R12312
Substrate
ATP [CPD:C00002];
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate [CPD:C22074];
2-oxoglutarate [CPD:C00026]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
staphyloferrin B [CPD:C22075]
Comment
Requires Mg2+. The enzyme, characterized from the bacterium Staphylococcus aureus, catalyses the last step in the biosynthesis of the siderophore staphyloferrin B. It belongs to a class of siderophore synthases known as type B nonribosomal peptide synthase-independent synthases (NIS). Type B NIS enzymes recognize the delta-acid group of 2-oxoglutarate. The enzyme forms a 2-oxoglutarate adenylate intermediate prior to ligation.
History
EC 6.3.2.56 created 2019
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K23375  staphyloferrin B synthase
Genes
DDDDda3937_02849(acsA)
DZEDd1591_2672
DDCDd586_2579
DZCW909_07430
DSOA4U42_16825
CEDLH89_04350
DFNCVE23_08430
DDQDDI_1279
DAQDAQ1742_01516(acsA)
DICDpoa569_002353
LBQCKQ53_11540
LPOPI6N93_10200
SGLSGP1_0039
XDOXDD1_1968
VTAA3468
PFVPsefu_2381
PSBPsyr_2589
PSYRN018_14365
PSPPSPPH_2756
PSAVPSA3335_16150
PAMGBKM19_016045
PAVLBKM03_14800
PVDCFBP1590__2719
PCABJGS08_13325
PCOFPOR16_12195
PTREI9H09_12715
PSYIMME58_13600
PCGAXG94_01640
PMEDE3Z27_14960
PBCCD58_14515
PCHEY04_15695
PCZPCL1606_28890
PCPJM49_14870
PSEMTO66_16010
PFKPFAS1_21435
PSILPMA3_09145
PALIA3K91_1227
PSYPE5677_07275
ABYABAYE2004
ABCACICU_01676
ABNAB57_1873
ABBABBFA_01824(iucC_1)
ABXABK1_2134
ABZABZJ_01837
ABRABTJ_02031
ABDABTW07_1892
ABHM3Q_2028
ABADABD1_16360
ABJBJAB07104_02076
ABABBJAB0715_01859
ABAJBJAB0868_01798
ABAZP795_9030
ABKLX00_08325
ABAUIX87_19075
ABAAIX88_10160
ABWBL01_08510
ABALABLAC_24820
ACCBDGL_001499
ANORR32_07475
ALCOTEC02_07350
ACALBUM88_11505
ASEII8T81_07360
ACDAOLE_07110
ACWA0J50_07095
ACVAMD27_03900
ARJDOM24_08490
AGUAS4_16150
ADSFPL17_05850
SDNSden_0588
CEKD0B88_14040
CEGD0C16_05370
HELHELO_3332
HHHCLM76_01220
HPIZGYM47_13040
HSVHNO53_08410
HAAA5892_12590
RSORSp0419
RSLRPSI07_mp0380
RSNRSPO_m01409
RSMCMR15_mp10432
RSEF504_3905
RSYRSUY_34870(iucC_1)
RPUCDC45_19725
RSGJK151_14175
CNCCNE_2c14850
CUHBJN34_08315
RMERmet_1115
BPLAbpln_2g05030
MFLAGO485_03170
NLCEBAPG3_006460
MNOMnod_6950
MAQUMaq22A_c25435(rhbC)
THACCSC3H3_23840
PCALBV455_03922(iucC_2)
LGYT479_22370
SALEEPH95_16145
SAUSA0114(sbnC)
SAVSAV0118
SAWSAHV_0117
SAHSaurJH1_0109
SAJSaurJH9_0105
SAMMW0091(sbnC)
SASSAS0092
SARSAR0121
SACSACOL0102
SAXUSA300HOU_0129
SAASAUSA300_0120(sbnC)
SAOSAOUHSC_00077
SAENWMN_0062(sbnC)
SADSAAV_0086
SUUM013TW_0108(sbnC)
SUVSAVC_00330
SUESAOV_0065
SUJSAA6159_00101(sbnC)
SUKSAA6008_00096(sbnC)
SUCECTR2_74
SUTSAT0131_00104
SUQHMPREF0772_10379
SUZMS7_0110
SUDST398NM01_0129
SUXSAEMRSA15_00840
SUWSATW20_01300(sbnC)
SUGSAPIG0129
SUFSARLGA251_00930(sbnC)
SAUASAAG_00602
SAUERSAU_000072(sbnC)
SAUNSAKOR_00092
SAUSSA40_0085(sbnC)
SAUUSA957_0100(sbnC)
SAUGSA268_0097(sbnC)
SAUZSAZ172_0129(sbnC)
SAUTSAI1T1_2000740
SAUJSAI2T2_1000740
SAUKSAI3T3_1000740
SAUQSAI4T8_1000740
SAUVSAI7S6_1000740
SAUWSAI5S5_1000740
SAUXSAI6T6_1000740
SAUYSAI8T7_1000740
SAUFX998_0097
SABSAB0057
SUYSA2981_0119(sbnC)
SAUBC248_0106
SAUMBN843_1200
SAUCCA347_129
SAURSABB_01722(sbnC)
SAUIAZ30_00615
SAUDCH52_05130
SAMSNI36_00510
SUHSAMSHR1132_00940
SSDSPSINT_0336
SDTSPSE_2122
SDPNCTC12225_02339(iucC_4)
SHUSHYC_00365
SSCHLH95_00365
SSCZRN70_00585
SAGQEP23_09760(sbnC)
SEQOSE1039_25090
SLZB5P37_07600
SARLSAP2_02050
SSHNCTC13712_00071(iucC_1)
SRAILN051_00240(sbnC)
SFFFOB90_07425(sbnC)
BAGRBA6348_15345
PPOLX809_04505
PTAHPL003_19630
PPEOABE82_04660
PNPIJ22_24480
POWIJ21_46060
PXLBS614_08700
PKBB4V02_14025
PAIHASL14_00100
PPSCEHS13_31640
PBACHUB98_21855
CCHLFPL14_15135
TUMCBW65_11400
TABCIG75_02855
LFBC1X05_04240
PABSJIR001_05110
SALJSMD11_0168
SACGFDZ84_26440
PMADBAY61_28580
 » show all
Reference
1  [PMID:19775248]
  Authors
Cheung J, Beasley FC, Liu S, Lajoie GA, Heinrichs DE
  Title
Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus.
  Journal
Mol Microbiol 74:594-608 (2009)
DOI:10.1111/j.1365-2958.2009.06880.x
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.56
IUBMB Enzyme Nomenclature: 6.3.2.56
ExPASy - ENZYME nomenclature database: 6.3.2.56
BRENDA, the Enzyme Database: 6.3.2.56

KEGG   REACTION: R12312
Entry
R12312                      Reaction                               
Name
2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate:2-oxoglutarate ligase (staphyloferrin B-forming)
Definition
ATP + 2-[(L-Alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate + 2-Oxoglutarate <=> AMP + Diphosphate + Staphyloferrin B
Equation
Enzyme
Pathway
rn00997  Biosynthesis of various other secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00875  Staphyloferrin B biosynthesis, L-serine => staphyloferrin B
Orthology
K23375  staphyloferrin B synthase [EC:6.3.2.56]

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