KEGG   PATHWAY: mmu03030
mmu03030                    Pathway                                

DNA replication - Mus musculus (mouse)
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Genetic Information Processing; Replication and repair
Pathway map
mmu03030  DNA replication

Mus musculus (mouse) [GN:mmu]
381760  Ssbp1; single-stranded DNA binding protein 1 [KO:K03111]
19819  Rnaseh1; ribonuclease H1 [KO:K03469] [EC:]
68275  Rpa1; replication protein A1 [KO:K07466]
18538  Pcna; proliferating cell nuclear antigen [KO:K04802]
327762  Dna2; DNA replication helicase/nuclease 2 [KO:K10742] [EC:]
14156  Fen1; flap structure specific endonuclease 1 [KO:K04799] [EC:3.-.-.-]
16881  Lig1; ligase I, DNA, ATP-dependent [KO:K10747] [EC:]
18968  Pola1; polymerase (DNA directed), alpha 1 [KO:K02320] [EC:]
18969  Pola2; polymerase (DNA directed), alpha 2 [KO:K02321]
19075  Prim1; DNA primase, p49 subunit [KO:K02684] [EC:]
19076  Prim2; DNA primase, p58 subunit [KO:K02685]
18971  Pold1; polymerase (DNA directed), delta 1, catalytic subunit [KO:K02327] [EC:]
18972  Pold2; polymerase (DNA directed), delta 2, regulatory subunit [KO:K02328]
67967  Pold3; polymerase (DNA-directed), delta 3, accessory subunit [KO:K03504]
69745  Pold4; polymerase (DNA-directed), delta 4 [KO:K03505]
18973  Pole; polymerase (DNA directed), epsilon [KO:K02324] [EC:]
18974  Pole2; polymerase (DNA directed), epsilon 2 (p59 subunit) [KO:K02325] [EC:]
59001  Pole3; polymerase (DNA directed), epsilon 3 (p17 subunit) [KO:K02326] [EC:]
66979  Pole4; polymerase (DNA-directed), epsilon 4 (p12 subunit) [KO:K03506] [EC:]
17216  Mcm2; minichromosome maintenance complex component 2 [KO:K02540] [EC:]
17215  Mcm3; minichromosome maintenance complex component 3 [KO:K02541] [EC:]
17217  Mcm4; minichromosome maintenance complex component 4 [KO:K02212] [EC:]
17218  Mcm5; minichromosome maintenance complex component 5 [KO:K02209] [EC:]
17219  Mcm6; minichromosome maintenance complex component 6 [KO:K02542] [EC:]
17220  Mcm7; minichromosome maintenance complex component 7 [KO:K02210] [EC:]
19891  Rpa2; replication protein A2 [KO:K10739]
68240  Rpa3; replication protein A3 [KO:K10740]
19687  Rfc1; replication factor C (activator 1) 1 [KO:K10754]
106344  Rfc4; replication factor C (activator 1) 4 [KO:K10755]
19718  Rfc2; replication factor C (activator 1) 2 [KO:K10755]
72151  Rfc5; replication factor C (activator 1) 5 [KO:K10756]
69263  Rfc3; replication factor C (activator 1) 3 [KO:K10756]
69724  Rnaseh2a; ribonuclease H2, large subunit [KO:K10743] [EC:]
67153  Rnaseh2b; ribonuclease H2, subunit B [KO:K10744]
68209  Rnaseh2c; ribonuclease H2, subunit C [KO:K10745]
Stillman B.
Smart machines at the DNA replication fork.
Cell 78:725-8 (1994)
Waga S, Stillman B.
The DNA replication fork in eukaryotic cells.
Annu Rev Biochem 67:721-51 (1998)
KO pathway

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