GenomeNet

Database: PDB
Entry: 1AFO
LinkDB: 1AFO
Original site: 1AFO 
HEADER    INTEGRAL MEMBRANE PROTEIN               11-MAR-97   1AFO              
TITLE     DIMERIC TRANSMEMBRANE DOMAIN OF HUMAN GLYCOPHORIN A, NMR,             
TITLE    2 20 STRUCTURES                                                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: GLYCOPHORIN A;                                             
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 FRAGMENT: TRANSMEMBRANE PEPTIDE;                                     
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 STRAIN: MG-T7;                                                       
SOURCE   6 ORGAN: PLASMA;                                                       
SOURCE   7 CELL: ERYTHROCYTE;                                                   
SOURCE   8 CELLULAR_LOCATION: PLASMA MEMBRANE;                                  
SOURCE   9 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  10 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  11 EXPRESSION_SYSTEM_STRAIN: MG-T7;                                     
SOURCE  12 EXPRESSION_SYSTEM_PLASMID: PET3A                                     
KEYWDS    INTEGRAL MEMBRANE PROTEIN, HUMAN GLYCOPHORIN A,                       
KEYWDS   2 TRANSMEMBRANE HELIX INTERACTIONS, MEMBRANE PROTEIN FOLDING           
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    K.R.MACKENZIE,J.H.PRESTEGARD,D.M.ENGELMAN                             
REVDAT   3   24-FEB-09 1AFO    1       VERSN                                    
REVDAT   2   01-APR-03 1AFO    1       JRNL                                     
REVDAT   1   17-SEP-97 1AFO    0                                                
JRNL        AUTH   K.R.MACKENZIE,J.H.PRESTEGARD,D.M.ENGELMAN                    
JRNL        TITL   A TRANSMEMBRANE HELIX DIMER: STRUCTURE AND                   
JRNL        TITL 2 IMPLICATIONS.                                                
JRNL        REF    SCIENCE                       V. 276   131 1997              
JRNL        REFN                   ISSN 0036-8075                               
JRNL        PMID   9082985                                                      
JRNL        DOI    10.1126/SCIENCE.276.5309.131                                 
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   K.R.MACKENZIE                                                
REMARK   1  TITL   STRUCTURE DETERMINATION OF THE DIMERIC MEMBRANE              
REMARK   1  TITL 2 SPANNING DOMAIN OF GLYCOPHORIN A IN DETERGENT                
REMARK   1  TITL 3 MICELLES BY TRIPLE RESONANCE NUCLEAR MAGNETIC                
REMARK   1  TITL 4 RESONANCE SPECTROSCOPY                                       
REMARK   1  REF    THESIS, YALE UNIVERSITY                    1996              
REMARK   1  PUBL   NEW HAVEN : YALE UNIVERSITY (THESIS)                         
REMARK   1  REFN                                                                
REMARK   1 REFERENCE 2                                                          
REMARK   1  AUTH   K.R.MACKENZIE,J.H.PRESTEGARD,D.M.ENGELMAN                    
REMARK   1  TITL   LEUCINE SIDE-CHAIN ROTAMERS IN A GLYCOPHORIN A               
REMARK   1  TITL 2 TRANSMEMBRANE PEPTIDE AS REVEALED BY THREE-BOND              
REMARK   1  TITL 3 CARBON-CARBON COUPLINGS AND 13C CHEMICAL SHIFTS              
REMARK   1  REF    J.BIOMOL.NMR                  V.   7   256 1996              
REMARK   1  REFN                   ISSN 0925-2738                               
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR 3.1                                           
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1AFO COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 313                                
REMARK 210  PH                             : 6.0                                
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : HNCA, CBCACONH, HCCH-TOCSY,        
REMARK 210                                   15N NOESY-HSQC, 13C NOESY-         
REMARK 210                                   HSQC, HNHA, SPIN-ECHO DIFF CT      
REMARK 210                                   HSQC                               
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 600 MHZ                   
REMARK 210  SPECTROMETER MODEL             : GE OMEGA500, VARIAN                
REMARK 210  SPECTROMETER MANUFACTURER      : GENERAL ELECTRIC, VARIAN           
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : X-PLOR 3.1                         
REMARK 210   METHOD USED                   : DISTANCE GEOMETRY SIMULATED        
REMARK 210                                   ANNEALING HYBRID                   
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 38                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : LEAST RESTRAINT VIOLATIONS         
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL                
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 LEU A  64     -165.82     46.11                                   
REMARK 500  1 ALA A  65       31.32   -163.00                                   
REMARK 500  1 HIS A  67      -53.27    170.12                                   
REMARK 500  1 SER A  69      174.92     51.29                                   
REMARK 500  1 ILE A  99     -112.97    -82.55                                   
REMARK 500  1 LYS A 100     -174.81     47.63                                   
REMARK 500  1 HIS B  66      114.11     77.93                                   
REMARK 500  1 HIS B  67       38.02    168.20                                   
REMARK 500  1 PHE B  68      -88.24   -141.98                                   
REMARK 500  1 GLU B  72      -73.71    -66.30                                   
REMARK 500  1 ILE B  99      102.18     94.28                                   
REMARK 500  2 GLN A  63      143.07    179.89                                   
REMARK 500  2 ALA A  65       60.50     64.15                                   
REMARK 500  2 HIS A  67       74.19   -152.35                                   
REMARK 500  2 PHE A  68       84.37    -58.74                                   
REMARK 500  2 SER A  69     -152.28   -104.06                                   
REMARK 500  2 GLU A  70      -55.38    168.68                                   
REMARK 500  2 PRO A  71       83.54    -58.12                                   
REMARK 500  2 GLU A  72      -72.18    -67.51                                   
REMARK 500  2 LEU B  64      159.23     67.71                                   
REMARK 500  2 ALA B  65       68.97   -165.87                                   
REMARK 500  2 SER B  69     -152.33   -150.57                                   
REMARK 500  2 GLU B  70       98.23     73.34                                   
REMARK 500  2 PRO B  71       58.48    -62.28                                   
REMARK 500  2 GLU B  72      -74.95    -65.15                                   
REMARK 500  2 LYS B 100     -162.83   -115.08                                   
REMARK 500  3 LEU A  64      173.51    -53.98                                   
REMARK 500  3 ALA A  65      104.28   -166.49                                   
REMARK 500  3 HIS A  66     -148.84    -70.32                                   
REMARK 500  3 HIS A  67     -175.60    -65.94                                   
REMARK 500  3 SER A  69      157.82    -46.62                                   
REMARK 500  3 GLU A  72      -74.68    -63.21                                   
REMARK 500  3 HIS B  67     -169.49   -163.84                                   
REMARK 500  3 PHE B  68      -56.15     75.94                                   
REMARK 500  3 PRO B  71      106.43    -59.72                                   
REMARK 500  4 ALA A  65       24.52   -144.24                                   
REMARK 500  4 HIS A  66       77.86     51.41                                   
REMARK 500  4 HIS A  67      -55.30   -158.88                                   
REMARK 500  4 PHE A  68      -62.92     68.38                                   
REMARK 500  4 SER A  69       48.12     76.17                                   
REMARK 500  4 GLU A  70      101.54     55.46                                   
REMARK 500  4 GLU A  72      -70.77    -59.29                                   
REMARK 500  4 GLN B  63      167.03     61.01                                   
REMARK 500  4 LEU B  64      -28.80    175.80                                   
REMARK 500  4 HIS B  66       63.82   -154.74                                   
REMARK 500  4 HIS B  67       69.29   -101.28                                   
REMARK 500  4 PHE B  68       90.23    -67.17                                   
REMARK 500  4 SER B  69      109.37     75.90                                   
REMARK 500  4 GLU B  72      -74.76    -48.54                                   
REMARK 500  4 ILE B  99     -131.52    -79.71                                   
REMARK 500  4 LYS B 100      136.47     59.47                                   
REMARK 500  5 HIS A  67      123.75     67.54                                   
REMARK 500  5 GLU A  70      -50.03    161.78                                   
REMARK 500  5 PRO A  71       75.96    -61.22                                   
REMARK 500  5 SER B  69       36.49    -89.81                                   
REMARK 500  5 GLU B  72      -72.16    -57.89                                   
REMARK 500  6 LEU A  64      -87.37     54.47                                   
REMARK 500  6 HIS A  66      -77.16    -56.16                                   
REMARK 500  6 HIS A  67      -69.04   -162.02                                   
REMARK 500  6 SER A  69       36.99   -172.98                                   
REMARK 500  6 SER B  69       15.12   -144.20                                   
REMARK 500  7 HIS A  66      -66.05     70.62                                   
REMARK 500  7 HIS A  67     -172.80     58.12                                   
REMARK 500  7 SER A  69       74.58     54.67                                   
REMARK 500  7 LYS A 100     -163.78   -123.02                                   
REMARK 500  7 LEU B  64       34.33   -160.98                                   
REMARK 500  7 HIS B  66      131.46     60.92                                   
REMARK 500  7 GLU B  70      -52.97    163.18                                   
REMARK 500  8 GLN A  63      159.64     57.37                                   
REMARK 500  8 ALA A  65       54.16   -114.98                                   
REMARK 500  8 HIS A  67       68.72    -68.29                                   
REMARK 500  8 PRO A  71       76.13    -59.06                                   
REMARK 500  8 PHE B  68      -74.54   -116.35                                   
REMARK 500  8 SER B  69      125.30     64.58                                   
REMARK 500  8 PRO B  71       96.62    -59.35                                   
REMARK 500  9 GLN A  63      159.40     60.44                                   
REMARK 500  9 ALA A  65      144.06     73.77                                   
REMARK 500  9 HIS A  66      -67.65   -147.66                                   
REMARK 500  9 HIS A  67       34.68   -166.88                                   
REMARK 500  9 PHE A  68      -67.71     65.90                                   
REMARK 500  9 GLU A  70       93.05    166.97                                   
REMARK 500  9 GLU A  72      -86.87    -64.65                                   
REMARK 500  9 ILE A  73      -39.30    -39.51                                   
REMARK 500  9 HIS B  66       96.42     51.85                                   
REMARK 500  9 HIS B  67      -72.32    -57.73                                   
REMARK 500 10 LEU A  64      -87.57     53.03                                   
REMARK 500 10 ALA A  65      -22.50    164.68                                   
REMARK 500 10 HIS A  66     -155.28   -103.02                                   
REMARK 500 10 HIS A  67      157.01     71.83                                   
REMARK 500 10 SER A  69     -170.76   -172.78                                   
REMARK 500 10 LEU B  64       37.01    -98.22                                   
REMARK 500 10 HIS B  66     -150.63     73.63                                   
REMARK 500 10 HIS B  67     -149.79   -145.27                                   
REMARK 500 10 SER B  69     -165.27    -59.58                                   
REMARK 500 10 PRO B  71       59.21    -62.64                                   
REMARK 500 11 GLN A  63       96.60     39.31                                   
REMARK 500 11 HIS A  66      120.55     71.04                                   
REMARK 500 11 HIS A  67     -154.22    -78.74                                   
REMARK 500 11 PRO A  71       73.22    -58.91                                   
REMARK 500 11 GLU A  72      -81.32    -64.83                                   
REMARK 500 11 LEU B  64       55.48   -144.78                                   
REMARK 500 11 PRO B  71       55.57    -65.17                                   
REMARK 500 11 GLU B  72      -71.97    -72.37                                   
REMARK 500 11 ILE B  99     -119.78    -82.27                                   
REMARK 500 11 LYS B 100       81.62     43.38                                   
REMARK 500 12 LEU A  64        7.16     80.44                                   
REMARK 500 12 ALA A  65       30.46    -90.59                                   
REMARK 500 12 HIS A  67       96.24     40.56                                   
REMARK 500 12 PHE A  68      164.87    -42.77                                   
REMARK 500 12 SER A  69      -94.08   -105.85                                   
REMARK 500 12 GLU A  70      -57.56    169.83                                   
REMARK 500 12 PRO A  71      -75.20    -54.82                                   
REMARK 500 12 GLU A  72     -131.50     34.25                                   
REMARK 500 12 ILE A  99     -118.79    -82.03                                   
REMARK 500 12 LYS A 100      141.12     61.19                                   
REMARK 500 12 ALA B  65       32.63    -95.22                                   
REMARK 500 12 HIS B  67       72.22     55.29                                   
REMARK 500 12 PHE B  68     -169.29    -59.02                                   
REMARK 500 12 SER B  69     -163.20   -168.56                                   
REMARK 500 12 GLU B  72      -76.77    -45.15                                   
REMARK 500 12 ILE B  99     -125.09    -77.06                                   
REMARK 500 13 HIS A  67      -84.00   -170.44                                   
REMARK 500 13 PRO A  71       57.73    -63.55                                   
REMARK 500 13 LEU B  64      109.35    -49.71                                   
REMARK 500 13 PHE B  68     -171.65    -61.73                                   
REMARK 500 14 GLN A  63      119.07   -165.59                                   
REMARK 500 14 PHE A  68       15.84   -150.10                                   
REMARK 500 14 ILE A  99     -103.27    -82.77                                   
REMARK 500 14 SER B  69       86.16    170.38                                   
REMARK 500 14 ILE B  99     -130.71    -76.61                                   
REMARK 500 14 LYS B 100      161.51     56.30                                   
REMARK 500 15 GLN A  63      172.81     71.13                                   
REMARK 500 15 LEU A  64       -4.82     81.34                                   
REMARK 500 15 HIS A  66      170.44     54.74                                   
REMARK 500 15 HIS A  67       23.23   -149.20                                   
REMARK 500 15 PHE A  68     -176.02     51.10                                   
REMARK 500 15 SER A  69       86.11     58.39                                   
REMARK 500 15 PRO A  71       92.75    -59.09                                   
REMARK 500 15 GLU A  72      -81.23    -68.44                                   
REMARK 500 15 ILE A  99      -81.49    -78.22                                   
REMARK 500 15 HIS B  66      142.61    170.21                                   
REMARK 500 15 PHE B  68     -171.04     48.60                                   
REMARK 500 16 LEU A  64      -76.60    -93.09                                   
REMARK 500 16 HIS A  67      -79.25   -123.99                                   
REMARK 500 16 PHE A  68     -175.62     75.45                                   
REMARK 500 16 SER A  69     -167.00   -175.55                                   
REMARK 500 16 GLU A  70      101.06     58.17                                   
REMARK 500 16 ILE A  99       39.14    -85.63                                   
REMARK 500 16 HIS B  67      129.98     66.82                                   
REMARK 500 16 GLU B  70      -57.17    172.48                                   
REMARK 500 17 HIS A  66       54.97   -163.05                                   
REMARK 500 17 PHE A  68     -162.89    -69.62                                   
REMARK 500 17 GLU A  70       70.46     46.83                                   
REMARK 500 17 PRO A  71       90.83    -60.33                                   
REMARK 500 17 GLU A  72      -92.13    -64.72                                   
REMARK 500 17 GLN B  63      124.62   -170.51                                   
REMARK 500 17 HIS B  67      -57.50   -135.08                                   
REMARK 500 17 PRO B  71       64.71    -61.71                                   
REMARK 500 17 GLU B  72      -73.86    -67.62                                   
REMARK 500 18 HIS A  66      -71.81   -114.44                                   
REMARK 500 18 HIS A  67      131.93    169.11                                   
REMARK 500 18 PHE A  68       38.40   -164.81                                   
REMARK 500 18 SER A  69     -151.78   -145.58                                   
REMARK 500 18 GLU A  70      -58.00    173.49                                   
REMARK 500 18 GLU A  72      -89.70    -65.65                                   
REMARK 500 18 ILE A  73      -38.79    -38.91                                   
REMARK 500 18 LEU B  64       75.19    -64.72                                   
REMARK 500 18 HIS B  66      177.04    -56.70                                   
REMARK 500 18 PHE B  68       59.49   -155.67                                   
REMARK 500 18 GLU B  70       95.25     49.41                                   
REMARK 500 18 PRO B  71       67.51    -60.53                                   
REMARK 500 18 GLU B  72      -72.39    -64.67                                   
REMARK 500 19 ALA A  65      -22.99    162.33                                   
REMARK 500 19 SER A  69     -165.44     70.09                                   
REMARK 500 19 PRO A  71       56.72    -63.95                                   
REMARK 500 19 GLU A  72      -72.72    -73.04                                   
REMARK 500 19 GLN B  63      143.26   -171.75                                   
REMARK 500 19 HIS B  66      175.09     56.28                                   
REMARK 500 19 HIS B  67      -49.30   -144.15                                   
REMARK 500 19 PHE B  68       52.22     39.41                                   
REMARK 500 19 SER B  69     -157.14   -118.49                                   
REMARK 500 19 LYS B 100       79.68   -115.84                                   
REMARK 500 20 GLN A  63       98.43     71.52                                   
REMARK 500 20 LEU A  64      -75.06   -118.77                                   
REMARK 500 20 HIS A  67     -150.76   -166.94                                   
REMARK 500 20 PRO A  71       76.55    -60.01                                   
REMARK 500 20 GLU A  72      -88.30    -61.55                                   
REMARK 500 20 GLN B  63      166.24    177.17                                   
REMARK 500 20 HIS B  66      167.09    -44.32                                   
REMARK 500 20 HIS B  67      -76.23    -80.50                                   
REMARK 500 20 PHE B  68     -158.40    -88.05                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 ARG A  96         0.31    SIDE_CHAIN                              
REMARK 500  1 ARG A  97         0.24    SIDE_CHAIN                              
REMARK 500  1 ARG B  96         0.25    SIDE_CHAIN                              
REMARK 500  1 ARG B  97         0.31    SIDE_CHAIN                              
REMARK 500  2 ARG A  96         0.23    SIDE_CHAIN                              
REMARK 500  2 ARG A  97         0.18    SIDE_CHAIN                              
REMARK 500  2 ARG B  96         0.19    SIDE_CHAIN                              
REMARK 500  2 ARG B  97         0.31    SIDE_CHAIN                              
REMARK 500  3 ARG A  96         0.20    SIDE_CHAIN                              
REMARK 500  3 ARG A  97         0.27    SIDE_CHAIN                              
REMARK 500  3 ARG B  96         0.32    SIDE_CHAIN                              
REMARK 500  3 ARG B  97         0.30    SIDE_CHAIN                              
REMARK 500  4 ARG A  96         0.12    SIDE_CHAIN                              
REMARK 500  4 ARG A  97         0.31    SIDE_CHAIN                              
REMARK 500  4 ARG B  96         0.30    SIDE_CHAIN                              
REMARK 500  4 ARG B  97         0.24    SIDE_CHAIN                              
REMARK 500  5 ARG A  96         0.29    SIDE_CHAIN                              
REMARK 500  5 ARG A  97         0.17    SIDE_CHAIN                              
REMARK 500  5 ARG B  96         0.30    SIDE_CHAIN                              
REMARK 500  5 ARG B  97         0.23    SIDE_CHAIN                              
REMARK 500  6 ARG A  96         0.32    SIDE_CHAIN                              
REMARK 500  6 ARG A  97         0.20    SIDE_CHAIN                              
REMARK 500  6 ARG B  96         0.27    SIDE_CHAIN                              
REMARK 500  6 ARG B  97         0.26    SIDE_CHAIN                              
REMARK 500  7 ARG A  96         0.27    SIDE_CHAIN                              
REMARK 500  7 ARG A  97         0.24    SIDE_CHAIN                              
REMARK 500  7 ARG B  96         0.28    SIDE_CHAIN                              
REMARK 500  7 ARG B  97         0.28    SIDE_CHAIN                              
REMARK 500  8 ARG A  96         0.24    SIDE_CHAIN                              
REMARK 500  8 ARG A  97         0.32    SIDE_CHAIN                              
REMARK 500  8 ARG B  96         0.31    SIDE_CHAIN                              
REMARK 500  8 ARG B  97         0.30    SIDE_CHAIN                              
REMARK 500  9 ARG A  96         0.17    SIDE_CHAIN                              
REMARK 500  9 ARG A  97         0.28    SIDE_CHAIN                              
REMARK 500  9 ARG B  96         0.30    SIDE_CHAIN                              
REMARK 500  9 ARG B  97         0.19    SIDE_CHAIN                              
REMARK 500 10 ARG A  96         0.23    SIDE_CHAIN                              
REMARK 500 10 ARG A  97         0.25    SIDE_CHAIN                              
REMARK 500 10 ARG B  96         0.32    SIDE_CHAIN                              
REMARK 500 10 ARG B  97         0.28    SIDE_CHAIN                              
REMARK 500 11 ARG A  96         0.19    SIDE_CHAIN                              
REMARK 500 11 ARG A  97         0.27    SIDE_CHAIN                              
REMARK 500 11 ARG B  96         0.24    SIDE_CHAIN                              
REMARK 500 11 ARG B  97         0.23    SIDE_CHAIN                              
REMARK 500 12 ARG A  96         0.30    SIDE_CHAIN                              
REMARK 500 12 ARG A  97         0.27    SIDE_CHAIN                              
REMARK 500 12 ARG B  96         0.31    SIDE_CHAIN                              
REMARK 500 12 ARG B  97         0.32    SIDE_CHAIN                              
REMARK 500 13 ARG A  96         0.28    SIDE_CHAIN                              
REMARK 500 13 ARG A  97         0.31    SIDE_CHAIN                              
REMARK 500 13 ARG B  96         0.27    SIDE_CHAIN                              
REMARK 500 13 ARG B  97         0.31    SIDE_CHAIN                              
REMARK 500 14 ARG A  96         0.14    SIDE_CHAIN                              
REMARK 500 14 ARG A  97         0.32    SIDE_CHAIN                              
REMARK 500 14 ARG B  96         0.23    SIDE_CHAIN                              
REMARK 500 14 ARG B  97         0.30    SIDE_CHAIN                              
REMARK 500 15 ARG A  96         0.29    SIDE_CHAIN                              
REMARK 500 15 ARG A  97         0.23    SIDE_CHAIN                              
REMARK 500 15 ARG B  96         0.20    SIDE_CHAIN                              
REMARK 500 16 ARG A  96         0.23    SIDE_CHAIN                              
REMARK 500 16 ARG A  97         0.27    SIDE_CHAIN                              
REMARK 500 16 ARG B  96         0.32    SIDE_CHAIN                              
REMARK 500 16 ARG B  97         0.26    SIDE_CHAIN                              
REMARK 500 17 ARG A  96         0.29    SIDE_CHAIN                              
REMARK 500 17 ARG A  97         0.25    SIDE_CHAIN                              
REMARK 500 17 ARG B  96         0.32    SIDE_CHAIN                              
REMARK 500 17 ARG B  97         0.31    SIDE_CHAIN                              
REMARK 500 18 ARG A  96         0.31    SIDE_CHAIN                              
REMARK 500 18 ARG A  97         0.26    SIDE_CHAIN                              
REMARK 500 18 ARG B  96         0.08    SIDE_CHAIN                              
REMARK 500 18 ARG B  97         0.26    SIDE_CHAIN                              
REMARK 500 19 ARG A  96         0.21    SIDE_CHAIN                              
REMARK 500 19 ARG A  97         0.25    SIDE_CHAIN                              
REMARK 500 19 ARG B  96         0.31    SIDE_CHAIN                              
REMARK 500 19 ARG B  97         0.30    SIDE_CHAIN                              
REMARK 500 20 ARG A  96         0.29    SIDE_CHAIN                              
REMARK 500 20 ARG A  97         0.29    SIDE_CHAIN                              
REMARK 500 20 ARG B  96         0.31    SIDE_CHAIN                              
REMARK 500 20 ARG B  97         0.31    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1AFO A   62   101  UNP    P02724   GLPA_HUMAN      81    120             
DBREF  1AFO B   62   101  UNP    P02724   GLPA_HUMAN      81    120             
SEQRES   1 A   40  VAL GLN LEU ALA HIS HIS PHE SER GLU PRO GLU ILE THR          
SEQRES   2 A   40  LEU ILE ILE PHE GLY VAL MET ALA GLY VAL ILE GLY THR          
SEQRES   3 A   40  ILE LEU LEU ILE SER TYR GLY ILE ARG ARG LEU ILE LYS          
SEQRES   4 A   40  LYS                                                          
SEQRES   1 B   40  VAL GLN LEU ALA HIS HIS PHE SER GLU PRO GLU ILE THR          
SEQRES   2 B   40  LEU ILE ILE PHE GLY VAL MET ALA GLY VAL ILE GLY THR          
SEQRES   3 B   40  ILE LEU LEU ILE SER TYR GLY ILE ARG ARG LEU ILE LYS          
SEQRES   4 B   40  LYS                                                          
HELIX    1   1 GLU A   72  LEU A   98  1                                  27    
HELIX    2   2 GLU B   72  LEU B   98  1                                  27    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system