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Database: PDB
Entry: 1EGW
LinkDB: 1EGW
Original site: 1EGW 
HEADER    TRANSCRIPTION/DNA                       17-FEB-00   1EGW              
TITLE     CRYSTAL STRUCTURE OF MEF2A CORE BOUND TO DNA                          
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DNA (5'-                                                   
COMPND   3 D(*AP*AP*AP*GP*CP*TP*AP*TP*TP*AP*TP*TP*AP*GP*CP*TP*T)-3');           
COMPND   4 CHAIN: E, H;                                                         
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: DNA (5'-                                                   
COMPND   8 D(*TP*AP*AP*GP*CP*TP*AP*AP*TP*AP*AP*TP*AP*GP*CP*TP*T)-3');           
COMPND   9 CHAIN: F, G;                                                         
COMPND  10 ENGINEERED: YES;                                                     
COMPND  11 MOL_ID: 3;                                                           
COMPND  12 MOLECULE: MADS BOX TRANSCRIPTION ENHANCER FACTOR 2, POLYPEPTIDE A;   
COMPND  13 CHAIN: A, B, C, D;                                                   
COMPND  14 FRAGMENT: N-TERMINUS, RESIDUES 2-78;                                 
COMPND  15 SYNONYM: MEF2A TRANSCRIPTION FACTOR, MYOCYTE ENHANCER FACTOR 2A,     
COMPND  16 MYOCYTE-SPECIFIC ENHANCER FACTOR 2A, SERUM RESPONSE FACTOR-LIKE      
COMPND  17 PROTEIN 1;                                                           
COMPND  18 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 OTHER_DETAILS: CONSENSUS DNA BINDING SITE FOR MEF2;                  
SOURCE   4 MOL_ID: 2;                                                           
SOURCE   5 SYNTHETIC: YES;                                                      
SOURCE   6 OTHER_DETAILS: CONSENSUS DNA BINDING SITE FOR MEF2;                  
SOURCE   7 MOL_ID: 3;                                                           
SOURCE   8 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   9 ORGANISM_COMMON: HUMAN;                                              
SOURCE  10 ORGANISM_TAXID: 9606;                                                
SOURCE  11 ORGAN: HEART, SKELETAL MUSCLE;                                       
SOURCE  12 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  13 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    MADS-BOX TRANSCRIPTION FACTOR, DNA-PROTEIN COMPLEX,                   
KEYWDS   2 TRANSCRIPTION/DNA, TRANSCRIPTION-DNA COMPLEX                         
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    E.SANTELLI,T.J.RICHMOND                                               
REVDAT   3   04-APR-18 1EGW    1       REMARK                                   
REVDAT   2   24-FEB-09 1EGW    1       VERSN                                    
REVDAT   1   20-MAR-00 1EGW    0                                                
JRNL        AUTH   E.SANTELLI,T.J.RICHMOND                                      
JRNL        TITL   CRYSTAL STRUCTURE OF MEF2A CORE BOUND TO DNA AT 1.5 A        
JRNL        TITL 2 RESOLUTION.                                                  
JRNL        REF    J.MOL.BIOL.                   V. 297   437 2000              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   10715212                                                     
JRNL        DOI    10.1006/JMBI.2000.3568                                       
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.50 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 0.9                                              
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,              
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : ENGH & HUBER                                    
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.50                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 20.00                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : 1092727.380                    
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : 0.0000                         
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 96.0                           
REMARK   3   NUMBER OF REFLECTIONS             : 86651                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM, CREATED IN P21          
REMARK   3   R VALUE            (WORKING SET) : 0.206                           
REMARK   3   FREE R VALUE                     : 0.229                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 10.000                          
REMARK   3   FREE R VALUE TEST SET COUNT      : 8679                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : 0.002                           
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 8                            
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.50                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.57                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 92.80                        
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : 9391                         
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2570                       
REMARK   3   BIN FREE R VALUE                    : 0.2860                       
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : 10.20                        
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : 1067                         
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : 0.009                        
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 2300                                    
REMARK   3   NUCLEIC ACID ATOMS       : 1382                                    
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 656                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 14.70                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 24.10                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -0.07000                                             
REMARK   3    B22 (A**2) : 0.18000                                              
REMARK   3    B33 (A**2) : -0.11000                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : -8.25000                                             
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : 0.19                            
REMARK   3   ESD FROM SIGMAA              (A) : 0.15                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : 5.00                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : 0.21                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : 0.16                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.010                           
REMARK   3   BOND ANGLES            (DEGREES) : 1.400                           
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : 19.80                           
REMARK   3   IMPROPER ANGLES        (DEGREES) : 1.460                           
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : RESTRAINED                                
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : 1.160 ; 2.000                
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : 1.850 ; 3.000                
REMARK   3   SIDE-CHAIN BOND              (A**2) : 1.760 ; 3.000                
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : 2.670 ; 4.000                
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : FLAT MODEL                                           
REMARK   3   KSOL        : 0.32                                                 
REMARK   3   BSOL        : 51.92                                                
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM                              
REMARK   3  PARAMETER FILE  2  : DNA-RNA_REP.PARAM                              
REMARK   3  PARAMETER FILE  3  : WATER_REP.PARAM                                
REMARK   3  PARAMETER FILE  4  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : PROTEIN.TOP                                    
REMARK   3  TOPOLOGY FILE  2   : DNA-RNA.TOP                                    
REMARK   3  TOPOLOGY FILE  3   : WATER.TOP                                      
REMARK   3  TOPOLOGY FILE  4   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: REFINED ALSO WITH REFMAC BY MURSHUDOV,    
REMARK   3  VAGIN, DODSON                                                       
REMARK   4                                                                      
REMARK   4 1EGW COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 22-FEB-00.                  
REMARK 100 THE DEPOSITION ID IS D_1000010558.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 18-MAY-99                          
REMARK 200  TEMPERATURE           (KELVIN) : 110                                
REMARK 200  PH                             : 5.8-6.3                            
REMARK 200  NUMBER OF CRYSTALS USED        : 3                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ESRF                               
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.932                              
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE                        
REMARK 200  DETECTOR MANUFACTURER          : MARRESEARCH                        
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 87264                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.500                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 30.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 96.0                               
REMARK 200  DATA REDUNDANCY                : 2.600                              
REMARK 200  R MERGE                    (I) : 0.07500                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 12.8000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.50                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.55                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 92.0                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 1.90                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.10400                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT AND    
REMARK 200  HEAVY ATOM PHASES FROM IODINE DERIVATIVE DATASET                    
REMARK 200 SOFTWARE USED: AMORE, MLPHARE                                        
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 50.84                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.50                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: PEG 6000, NACL, BA(NO3)2, SODIUM         
REMARK 280  ACETATE, BIS-TRIS BUFFER, DTT, VAPOR DIFFUSION, HANGING DROP,       
REMARK 280  TEMPERATURE 295K                                                    
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1                              
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 300 REMARK: A PROTEIN DIMER BOUND TO A DOUBLE STRANDED DNA               
REMARK 300 OLIGONUCLEOTIDE FULLY DESCRIBED BY THE DEPOSITED COORDINATES         
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: E, F, A, B                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: G, H, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     ASN A    73                                                      
REMARK 465     GLU A    74                                                      
REMARK 465     PRO A    75                                                      
REMARK 465     HIS A    76                                                      
REMARK 465     GLU A    77                                                      
REMARK 465     SER A    78                                                      
REMARK 465     GLU B    74                                                      
REMARK 465     PRO B    75                                                      
REMARK 465     HIS B    76                                                      
REMARK 465     GLU B    77                                                      
REMARK 465     SER B    78                                                      
REMARK 465     GLU C    74                                                      
REMARK 465     PRO C    75                                                      
REMARK 465     HIS C    76                                                      
REMARK 465     GLU C    77                                                      
REMARK 465     SER C    78                                                      
REMARK 465     ASN D    73                                                      
REMARK 465     GLU D    74                                                      
REMARK 465     PRO D    75                                                      
REMARK 465     HIS D    76                                                      
REMARK 465     GLU D    77                                                      
REMARK 465     SER D    78                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LYS A   4    CG   CD   CE   NZ                                   
REMARK 470     ASP A  61    CG   OD1  OD2                                       
REMARK 470     ASP A  63    CG   OD1  OD2                                       
REMARK 470     LYS A  64    CG   CD   CE   NZ                                   
REMARK 470     LEU A  67    CG   CD1  CD2                                       
REMARK 470     LYS B   4    CG   CD   CE   NZ                                   
REMARK 470     ASP B  61    CG   OD1  OD2                                       
REMARK 470     ASP B  63    CG   OD1  OD2                                       
REMARK 470     LYS B  64    CG   CD   CE   NZ                                   
REMARK 470     LEU B  67    CG   CD1  CD2                                       
REMARK 470     GLU B  71    CG   CD   OE1  OE2                                  
REMARK 470     LYS C   4    CG   CD   CE   NZ                                   
REMARK 470     ASP C  61    CG   OD1  OD2                                       
REMARK 470     ASP C  63    CG   OD1  OD2                                       
REMARK 470     LYS C  64    CG   CD   CE   NZ                                   
REMARK 470     LEU C  67    CG   CD1  CD2                                       
REMARK 470     GLU C  71    CG   CD   OE1  OE2                                  
REMARK 470     LYS D   4    CG   CD   CE   NZ                                   
REMARK 470     ASP D  61    CG   OD1  OD2                                       
REMARK 470     ASP D  63    CG   OD1  OD2                                       
REMARK 470     LYS D  64    CG   CD   CE   NZ                                   
REMARK 470     LEU D  67    CG   CD1  CD2                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   N4    DC E    15     O    HOH E  1233              2.19            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500     DG E   4   C3' -  C2' -  C1' ANGL. DEV. =  -5.0 DEGREES          
REMARK 500     DG E   4   O4' -  C1' -  N9  ANGL. DEV. =   2.2 DEGREES          
REMARK 500     DG F   4   O4' -  C1' -  N9  ANGL. DEV. =   2.0 DEGREES          
REMARK 500     DG G   4   O4' -  C1' -  N9  ANGL. DEV. =   2.3 DEGREES          
REMARK 500     DG H   4   C3' -  C2' -  C1' ANGL. DEV. =  -5.1 DEGREES          
REMARK 500     DG H   4   O4' -  C1' -  N9  ANGL. DEV. =   2.4 DEGREES          
REMARK 500    THR C   9   O   -  C   -  N   ANGL. DEV. = -10.2 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500     DA E   2         0.06    SIDE CHAIN                              
REMARK 500     DG E   4         0.07    SIDE CHAIN                              
REMARK 500     DC E   5         0.06    SIDE CHAIN                              
REMARK 500     DG F   4         0.07    SIDE CHAIN                              
REMARK 500     DA F   7         0.06    SIDE CHAIN                              
REMARK 500     DG G   4         0.06    SIDE CHAIN                              
REMARK 500     DC G   5         0.07    SIDE CHAIN                              
REMARK 500     DA G   7         0.06    SIDE CHAIN                              
REMARK 500     DA H   2         0.06    SIDE CHAIN                              
REMARK 500     DG H   4         0.07    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: MAIN CHAIN PLANARITY                                       
REMARK 500                                                                      
REMARK 500 THE FOLLOWING RESIDUES HAVE A PSEUDO PLANARITY                       
REMARK 500 TORSION ANGLE, C(I) - CA(I) - N(I+1) - O(I), GREATER                 
REMARK 500 10.0 DEGREES. (M=MODEL NUMBER; RES=RESIDUE NAME;                     
REMARK 500 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;                            
REMARK 500 I=INSERTION CODE).                                                   
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        ANGLE                                           
REMARK 500    THR A   9         10.72                                           
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1EGW A    2    78  UNP    Q02078   MEF2A_HUMAN      2     78             
DBREF  1EGW B    2    78  UNP    Q02078   MEF2A_HUMAN      2     78             
DBREF  1EGW C    2    78  UNP    Q02078   MEF2A_HUMAN      2     78             
DBREF  1EGW D    2    78  UNP    Q02078   MEF2A_HUMAN      2     78             
DBREF  1EGW E    1    17  PDB    1EGW     1EGW             1     17             
DBREF  1EGW F    1    17  PDB    1EGW     1EGW             1     17             
DBREF  1EGW G    1    17  PDB    1EGW     1EGW             1     17             
DBREF  1EGW H    1    17  PDB    1EGW     1EGW             1     17             
SEQRES   1 E   17   DA  DA  DA  DG  DC  DT  DA  DT  DT  DA  DT  DT  DA          
SEQRES   2 E   17   DG  DC  DT  DT                                              
SEQRES   1 F   17   DT  DA  DA  DG  DC  DT  DA  DA  DT  DA  DA  DT  DA          
SEQRES   2 F   17   DG  DC  DT  DT                                              
SEQRES   1 G   17   DT  DA  DA  DG  DC  DT  DA  DA  DT  DA  DA  DT  DA          
SEQRES   2 G   17   DG  DC  DT  DT                                              
SEQRES   1 H   17   DA  DA  DA  DG  DC  DT  DA  DT  DT  DA  DT  DT  DA          
SEQRES   2 H   17   DG  DC  DT  DT                                              
SEQRES   1 A   77  GLY ARG LYS LYS ILE GLN ILE THR ARG ILE MET ASP GLU          
SEQRES   2 A   77  ARG ASN ARG GLN VAL THR PHE THR LYS ARG LYS PHE GLY          
SEQRES   3 A   77  LEU MET LYS LYS ALA TYR GLU LEU SER VAL LEU CYS ASP          
SEQRES   4 A   77  CYS GLU ILE ALA LEU ILE ILE PHE ASN SER SER ASN LYS          
SEQRES   5 A   77  LEU PHE GLN TYR ALA SER THR ASP MET ASP LYS VAL LEU          
SEQRES   6 A   77  LEU LYS TYR THR GLU TYR ASN GLU PRO HIS GLU SER              
SEQRES   1 B   77  GLY ARG LYS LYS ILE GLN ILE THR ARG ILE MET ASP GLU          
SEQRES   2 B   77  ARG ASN ARG GLN VAL THR PHE THR LYS ARG LYS PHE GLY          
SEQRES   3 B   77  LEU MET LYS LYS ALA TYR GLU LEU SER VAL LEU CYS ASP          
SEQRES   4 B   77  CYS GLU ILE ALA LEU ILE ILE PHE ASN SER SER ASN LYS          
SEQRES   5 B   77  LEU PHE GLN TYR ALA SER THR ASP MET ASP LYS VAL LEU          
SEQRES   6 B   77  LEU LYS TYR THR GLU TYR ASN GLU PRO HIS GLU SER              
SEQRES   1 C   77  GLY ARG LYS LYS ILE GLN ILE THR ARG ILE MET ASP GLU          
SEQRES   2 C   77  ARG ASN ARG GLN VAL THR PHE THR LYS ARG LYS PHE GLY          
SEQRES   3 C   77  LEU MET LYS LYS ALA TYR GLU LEU SER VAL LEU CYS ASP          
SEQRES   4 C   77  CYS GLU ILE ALA LEU ILE ILE PHE ASN SER SER ASN LYS          
SEQRES   5 C   77  LEU PHE GLN TYR ALA SER THR ASP MET ASP LYS VAL LEU          
SEQRES   6 C   77  LEU LYS TYR THR GLU TYR ASN GLU PRO HIS GLU SER              
SEQRES   1 D   77  GLY ARG LYS LYS ILE GLN ILE THR ARG ILE MET ASP GLU          
SEQRES   2 D   77  ARG ASN ARG GLN VAL THR PHE THR LYS ARG LYS PHE GLY          
SEQRES   3 D   77  LEU MET LYS LYS ALA TYR GLU LEU SER VAL LEU CYS ASP          
SEQRES   4 D   77  CYS GLU ILE ALA LEU ILE ILE PHE ASN SER SER ASN LYS          
SEQRES   5 D   77  LEU PHE GLN TYR ALA SER THR ASP MET ASP LYS VAL LEU          
SEQRES   6 D   77  LEU LYS TYR THR GLU TYR ASN GLU PRO HIS GLU SER              
FORMUL   9  HOH   *656(H2 O)                                                    
HELIX    1   1 ASP A   13  CYS A   39  1                                  27    
HELIX    2   2 ASP A   61  TYR A   72  1                                  12    
HELIX    3   3 ASP B   13  CYS B   39  1                                  27    
HELIX    4   4 ASP B   61  ASN B   73  1                                  13    
HELIX    5   5 ASP C   13  ASP C   40  1                                  28    
HELIX    6   6 ASP C   61  ASN C   73  1                                  13    
HELIX    7   7 ASP D   13  CYS D   39  1                                  27    
HELIX    8   8 ASP D   61  GLU D   71  1                                  11    
SHEET    1   A 4 LEU A  54  ALA A  58  0                                        
SHEET    2   A 4 GLU A  42  PHE A  48 -1  N  LEU A  45   O  TYR A  57           
SHEET    3   A 4 GLU B  42  PHE B  48 -1  O  GLU B  42   N  PHE A  48           
SHEET    4   A 4 LEU B  54  ALA B  58 -1  O  PHE B  55   N  ILE B  47           
SHEET    1   B 4 LEU C  54  ALA C  58  0                                        
SHEET    2   B 4 GLU C  42  PHE C  48 -1  N  LEU C  45   O  TYR C  57           
SHEET    3   B 4 GLU D  42  PHE D  48 -1  O  GLU D  42   N  PHE C  48           
SHEET    4   B 4 LEU D  54  ALA D  58 -1  O  PHE D  55   N  ILE D  47           
CRYST1   41.371   60.696   63.987 115.18  89.99  90.00 P 1           4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.024172  0.000001 -0.000006        0.00000                         
SCALE2      0.000000  0.016476  0.007745        0.00000                         
SCALE3      0.000000  0.000000  0.017269        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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