GenomeNet

Database: PDB
Entry: 1GNC
LinkDB: 1GNC
Original site: 1GNC 
HEADER    GROWTH FACTOR                           08-MAR-94   1GNC              
TITLE     STRUCTURE AND DYNAMICS OF THE HUMAN GRANULOCYTE COLONY-STIMULATING    
TITLE    2 FACTOR DETERMINED BY NMR SPECTROSCOPY. LOOP MOBILITY IN A FOUR-HELIX-
TITLE    3 BUNDLE PROTEIN                                                       
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: GRANULOCYTE COLONY-STIMULATING FACTOR;                     
COMPND   3 CHAIN: A;                                                            
COMPND   4 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606                                                 
KEYWDS    GROWTH FACTOR                                                         
EXPDTA    SOLUTION NMR                                                          
NUMMDL    10                                                                    
AUTHOR    T.W.ZINK,A.ROSS,R.RUDOLPH,T.A.HOLAK                                   
REVDAT   3   29-NOV-17 1GNC    1       REMARK HELIX                             
REVDAT   2   24-FEB-09 1GNC    1       VERSN                                    
REVDAT   1   31-JUL-94 1GNC    0                                                
JRNL        AUTH   T.ZINK,A.ROSS,K.LUERS,C.CIESLAR,R.RUDOLPH,T.A.HOLAK          
JRNL        TITL   STRUCTURE AND DYNAMICS OF THE HUMAN GRANULOCYTE              
JRNL        TITL 2 COLONY-STIMULATING FACTOR DETERMINED BY NMR SPECTROSCOPY.    
JRNL        TITL 3 LOOP MOBILITY IN A FOUR-HELIX-BUNDLE PROTEIN.                
JRNL        REF    BIOCHEMISTRY                  V.  33  8453 1994              
JRNL        REFN                   ISSN 0006-2960                               
JRNL        PMID   7518249                                                      
JRNL        DOI    10.1021/BI00194A009                                          
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   T.ZINK,A.ROSS,K.LUERS,C.CIESLER,R.RUDOLPH,T.A.HOLAK          
REMARK   1  TITL   SECONDARY STRUCTURE OF HUMAN GRANULOCYTE COLONY STIMULATING  
REMARK   1  TITL 2 FACTOR DERIVED FROM NMR SPECTROSCOPY                         
REMARK   1  REF    FEBS LETT.                    V. 314   435 1992              
REMARK   1  REFN                   ISSN 0014-5793                               
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR                                               
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1GNC COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 100 THE DEPOSITION ID IS D_1000173629.                                   
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : NULL                               
REMARK 210  PH                             : NULL                               
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NULL                               
REMARK 210  SPECTROMETER FIELD STRENGTH    : NULL                               
REMARK 210  SPECTROMETER MODEL             : NULL                               
REMARK 210  SPECTROMETER MANUFACTURER      : NULL                               
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NULL                               
REMARK 210   METHOD USED                   : NULL                               
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : NULL                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : NULL                               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL                
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER;                   
REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                            
REMARK 465   MODELS 1-10                                                        
REMARK 465     RES C SSSEQI                                                     
REMARK 465     VAL A    36A                                                     
REMARK 465     SER A    36B                                                     
REMARK 465     GLU A    36C                                                     
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 THR A   2      113.02   -161.06                                   
REMARK 500  1 SER A   8       81.71   -150.46                                   
REMARK 500  1 GLN A  12      -58.66   -175.56                                   
REMARK 500  1 TYR A  40      -74.13    -86.73                                   
REMARK 500  1 LYS A  41      -75.40    -91.73                                   
REMARK 500  1 LEU A  42      -39.16   -179.77                                   
REMARK 500  1 PRO A  45     -160.10    -58.65                                   
REMARK 500  1 GLU A  47      -64.01    179.93                                   
REMARK 500  1 ILE A  57      138.11     67.87                                   
REMARK 500  1 PRO A  61     -160.93    -66.95                                   
REMARK 500  1 SER A  64      -67.94    -96.72                                   
REMARK 500  1 PRO A  66      -77.07    -63.72                                   
REMARK 500  1 ALA A  69       69.29   -174.75                                   
REMARK 500  1 LEU A  70       97.29   -173.77                                   
REMARK 500  1 GLN A  71      111.23     59.81                                   
REMARK 500  1 ILE A  96      -81.99    -52.17                                   
REMARK 500  1 SER A  97      -56.98    169.82                                   
REMARK 500  1 PRO A  98       86.65    -60.31                                   
REMARK 500  1 LEU A 125       82.67    176.58                                   
REMARK 500  1 ALA A 130       52.60   -119.62                                   
REMARK 500  1 PRO A 133     -161.09    -66.33                                   
REMARK 500  1 GLN A 135       80.55    170.71                                   
REMARK 500  1 ALA A 137      124.50    164.19                                   
REMARK 500  1 MET A 138      -56.58    179.65                                   
REMARK 500  1 PRO A 139       83.94    -66.94                                   
REMARK 500  1 ALA A 140       99.64    168.00                                   
REMARK 500  1 ALA A 142       -8.74    102.20                                   
REMARK 500  1 SER A 143      111.60     64.25                                   
REMARK 500  1 ALA A 144     -158.30    174.95                                   
REMARK 500  2 PRO A   6       96.51    -59.17                                   
REMARK 500  2 ALA A   7      125.59    177.56                                   
REMARK 500  2 LEU A  10       64.49   -173.23                                   
REMARK 500  2 PRO A  11      -75.15    -62.38                                   
REMARK 500  2 LYS A  41      -39.22    -15.89                                   
REMARK 500  2 HIS A  44       73.99     67.37                                   
REMARK 500  2 GLU A  46      175.42     52.68                                   
REMARK 500  2 GLU A  47      -64.06     82.52                                   
REMARK 500  2 ILE A  57      139.83     67.33                                   
REMARK 500  2 PRO A  58      157.59    -46.28                                   
REMARK 500  2 SER A  64     -178.08    -57.98                                   
REMARK 500  2 PRO A  66      -80.16    -67.45                                   
REMARK 500  2 ALA A  69       67.75   -165.46                                   
REMARK 500  2 LEU A  70       86.92     61.17                                   
REMARK 500  2 GLN A  71      100.05    -59.35                                   
REMARK 500  2 LEU A  72      -61.45   -178.19                                   
REMARK 500  2 PRO A 102       87.19    -63.22                                   
REMARK 500  2 THR A 103      -66.55   -168.42                                   
REMARK 500  2 LEU A 125       82.39    175.12                                   
REMARK 500  2 PRO A 129     -179.08    -61.89                                   
REMARK 500  2 ALA A 130       51.81   -153.88                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS     287 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1GNC A    2   175  UNP    P09919   CSF3_HUMAN      31    207             
SEQRES   1 A  178  MET THR PRO LEU GLY PRO ALA SER SER LEU PRO GLN SER          
SEQRES   2 A  178  PHE LEU LEU LYS CYS LEU GLU GLN VAL ARG LYS ILE GLN          
SEQRES   3 A  178  GLY ASP GLY ALA ALA LEU GLN GLU LYS LEU VAL SER GLU          
SEQRES   4 A  178  CYS ALA THR TYR LYS LEU CYS HIS PRO GLU GLU LEU VAL          
SEQRES   5 A  178  LEU LEU GLY HIS SER LEU GLY ILE PRO TRP ALA PRO LEU          
SEQRES   6 A  178  SER SER CYS PRO SER GLN ALA LEU GLN LEU ALA GLY CYS          
SEQRES   7 A  178  LEU SER GLN LEU HIS SER GLY LEU PHE LEU TYR GLN GLY          
SEQRES   8 A  178  LEU LEU GLN ALA LEU GLU GLY ILE SER PRO GLU LEU GLY          
SEQRES   9 A  178  PRO THR LEU ASP THR LEU GLN LEU ASP VAL ALA ASP PHE          
SEQRES  10 A  178  ALA THR THR ILE TRP GLN GLN MET GLU GLU LEU GLY MET          
SEQRES  11 A  178  ALA PRO ALA LEU GLN PRO THR GLN GLY ALA MET PRO ALA          
SEQRES  12 A  178  PHE ALA SER ALA PHE GLN ARG ARG ALA GLY GLY VAL LEU          
SEQRES  13 A  178  VAL ALA SER HIS LEU GLN SER PHE LEU GLU VAL SER TYR          
SEQRES  14 A  178  ARG VAL LEU ARG HIS LEU ALA GLN PRO                          
HELIX    1   A GLN A   12  LEU A   42  1                                  31    
HELIX    2   B LEU A   72  GLY A   95  1                                  24    
HELIX    3   C THR A  103  GLY A  126  1                                  24    
HELIX    4   D GLN A  146  HIS A  171  1                                  26    
HELIX    5   E LEU A   48  ILE A   57  1                                  10    
SSBOND   1 CYS A   37    CYS A   43                          1555   1555  2.02  
SSBOND   2 CYS A   65    CYS A   75                          1555   1555  2.02  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system