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Database: PDB
Entry: 1GTW
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Original site: 1GTW 
HEADER    TRANSCRIPTION/DNA                       22-JAN-02   1GTW              
TITLE     CRYSTAL STRUCTURE OF C/EBPBETA BZIP HOMODIMER BOUND TO A              
TITLE    2 DNA FRAGMENT FROM THE TOM-1A PROMOTER                                
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CAAT/ENHANCER BINDING PROTEIN BETA;                        
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 FRAGMENT: BZIP DOMAIN, RESIDUES 259-336;                             
COMPND   5 SYNONYM: C/EBP BETA, LIVER ACTIVATOR PROTEIN, LAP,                   
COMPND   6  LIVER-ENRICHED INHIBITORY PROTEIN, LIP, NUCLEAR FACTOR NF-IL6,      
COMPND   7  TRANSCRIPTION FACTOR 5, TCF-5;                                      
COMPND   8 ENGINEERED: YES;                                                     
COMPND   9 MOL_ID: 2;                                                           
COMPND  10 MOLECULE: 5'-D(*AP*AP*TP*GP*TP*GP*GP*CP*GP*CP*                       
COMPND  11  AP*AP*TP*CP*CP*T)-3';                                               
COMPND  12 CHAIN: C;                                                            
COMPND  13 OTHER_DETAILS: TOM-1A PROMOTER;                                      
COMPND  14 MOL_ID: 3;                                                           
COMPND  15 MOLECULE: 5'-D(*TP*AP*GP*GP*AP*TP*TP*GP*CP*GP*                       
COMPND  16  CP*CP*AP*CP*AP*T)-3';                                               
COMPND  17 CHAIN: D;                                                            
COMPND  18 OTHER_DETAILS: TOM-1A PROMOTER                                       
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PAR2156;                                  
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 SYNTHETIC: YES;                                                      
SOURCE  12 MOL_ID: 3;                                                           
SOURCE  13 SYNTHETIC: YES                                                       
KEYWDS    TRANSCRIPTION/DNA, PROTEIN-DNA COMPLEX, TRANSCRIPTION                 
KEYWDS   2 FACTOR, C/EBP, HYPOTHETICAL PROTEIN                                  
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    T.H.TAHIROV,K.OGATA                                                   
REVDAT   3   22-JAN-14 1GTW    1       TITLE  COMPND KEYWDS REMARK              
REVDAT   3 2                           VERSN  DBREF  FORMUL                     
REVDAT   2   24-FEB-09 1GTW    1       VERSN                                    
REVDAT   1   06-FEB-04 1GTW    0                                                
JRNL        AUTH   T.H.TAHIROV,T.INOUE-BUNGO,K.SATO,M.SASAKI,K.OGATA            
JRNL        TITL   STRUCTURAL BASIS FOR FLEXIBLE BASE RECOGNITION BY            
JRNL        TITL 2 C/EBPBETA                                                    
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   T.H.TAHIROV,K.SATO,E.ICHIKAWA-IWATA,M.SASAKI,                
REMARK   1  AUTH 2 T.INOUE-BUNGO,M.SHIINA,K.KIMURA,S.TAKATA,                    
REMARK   1  AUTH 3 A.FUJIKAWA,H.MORII,T.KUMASAKA,M.YAMAMOTO,S.ISHII,            
REMARK   1  AUTH 4 K.OGATA                                                      
REMARK   1  TITL   MECHANISM OF C-MYB-C/EBPBETA COOPERATION FROM                
REMARK   1  TITL 2 SEPARATED SITES ON A PROMOTER                                
REMARK   1  REF    CELL (CAMBRIDGE,MASS.)        V. 108    57 2002              
REMARK   1  REFN                   ISSN 0092-8674                               
REMARK   1  PMID   11792321                                                     
REMARK   1  DOI    10.1016/S0092-8674(01)00636-5                                
REMARK   1 REFERENCE 2                                                          
REMARK   1  AUTH   T.H.TAHIROV,T.INOUE-BUNGO,M.SASAKI,A.FUJIKAWA,               
REMARK   1  AUTH 2 K.KIMURA,K.SATO,S.ADACHI,K.KAMIYAOGATA                       
REMARK   1  TITL   CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSIS OF            
REMARK   1  TITL 2 THE C/EBPBETA C-TERMINAL REGION IN COMPLEX WITH              
REMARK   1  TITL 3 DNA                                                          
REMARK   1  REF    ACTA CRYSTALLOGR.,SECT.D      V.  57   854 2001              
REMARK   1  REFN                   ISSN 0907-4449                               
REMARK   1  PMID   11375506                                                     
REMARK   1  DOI    10.1107/S0907444901003912                                    
REMARK   1 REFERENCE 3                                                          
REMARK   1  AUTH   T.H.TAHIROV,T.INOUE-BUNGO,H.MORII,A.FUJIKAWA,                
REMARK   1  AUTH 2 M.SASAKI,K.KIMURA,M.SHIINA,K.SATO,T.KUMASAKA,                
REMARK   1  AUTH 3 M.YAMAMOTO,S.ISHII,K.OGATA                                   
REMARK   1  TITL   STRUCTURAL ANALYSES OF DNA RECOGNITION BY THE                
REMARK   1  TITL 2 AML1/ RUNX-1 RUNT DOMAIN AND ITS ALLOSTERIC                  
REMARK   1  TITL 3 CONTROL BY CBFBETA                                           
REMARK   1  REF    CELL (CAMBRIDGE,MASS.)        V. 104   755 2001              
REMARK   1  REFN                   ISSN 0092-8674                               
REMARK   1  PMID   11257229                                                     
REMARK   1  DOI    10.1016/S0092-8674(01)00271-9                                
REMARK   1 REFERENCE 4                                                          
REMARK   1  AUTH   T.H.TAHIROV,T.INOUE,M.SASAKI,M.SHIINA,K.KIMURA,              
REMARK   1  AUTH 2 K.SATO,T.KUMASAKA,M.YAMAMOTO,N.KAMIYA,K.OGATA                
REMARK   1  TITL   CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSES OF            
REMARK   1  TITL 2 QUATERNARY, TERNARY AND BINARY PROTEIN-DNA                   
REMARK   1  TITL 3 COMPLEXES WITH INVOLVEMENT OF AML1/RUNX-1/CBFALPHA           
REMARK   1  TITL 4 RUNT DOMAIN, CBFBETA AND THE C/EBPBETA BZIP REGION           
REMARK   1  REF    ACTA CRYSTALLOGR.,SECT.D      V.  57   850 2001              
REMARK   1  REFN                   ISSN 0907-4449                               
REMARK   1  PMID   11375505                                                     
REMARK   1  DOI    10.1107/S0907444901003900                                    
REMARK   1 REFERENCE 5                                                          
REMARK   1  AUTH   T.H.TAHIROV,T.INOUE,M.SASAKI,M.SHIINA,K.KIMURA,              
REMARK   1  AUTH 2 K.SATO,T.KUMASAKA,M.YAMAMOTO,N.KAMIYA,K.OGATA                
REMARK   1  TITL   CRYSTALS OF TERNARY PROTEIN-DNA COMPLEXES COMPOSED           
REMARK   1  TITL 2 OF DNA-BINDING DOMAINS C-MYB OR V-MYB, C/EBPALPHA            
REMARK   1  TITL 3 OR C/EBPBETA AND TOM-1A PROMOTER FRAGMENT                    
REMARK   1  REF    ACTA CRYSTALLOGR.,SECT.D      V.  57  1655 2001              
REMARK   1  REFN                   ISSN 0907-4449                               
REMARK   1  PMID   11679735                                                     
REMARK   1  DOI    10.1107/S0907444901011982                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.85 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.0                                              
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,              
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : NULL                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.85                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.87                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : 327560.56                      
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL                           
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 98.0                           
REMARK   3   NUMBER OF REFLECTIONS             : 35717                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.231                           
REMARK   3   FREE R VALUE                     : 0.271                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.1                             
REMARK   3   FREE R VALUE TEST SET COUNT      : 1809                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : 0.006                           
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 6                            
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.85                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.97                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 94.9                         
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : 5402                         
REMARK   3   BIN R VALUE           (WORKING SET) : 0.378                        
REMARK   3   BIN FREE R VALUE                    : 0.432                        
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : 5.0                          
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : 282                          
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : 0.026                        
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 1129                                    
REMARK   3   NUCLEIC ACID ATOMS       : 650                                     
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 316                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 34.2                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 42.9                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 7.95                                                 
REMARK   3    B22 (A**2) : -5.16                                                
REMARK   3    B33 (A**2) : -2.79                                                
REMARK   3    B12 (A**2) : 0.00                                                 
REMARK   3    B13 (A**2) : 0.00                                                 
REMARK   3    B23 (A**2) : 0.00                                                 
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : 0.27                            
REMARK   3   ESD FROM SIGMAA              (A) : 0.39                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : 20.00                           
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : 0.32                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : 0.44                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.004                           
REMARK   3   BOND ANGLES            (DEGREES) : 1.0                             
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : 15.7                            
REMARK   3   IMPROPER ANGLES        (DEGREES) : 1.13                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : RESTRAINED                                
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : 4.97  ; 1.50                 
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : 5.68  ; 2.00                 
REMARK   3   SIDE-CHAIN BOND              (A**2) : 6.94  ; 2.00                 
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : 9.23  ; 2.50                 
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : FLAT MODEL                                           
REMARK   3   KSOL        : 0.348766                                             
REMARK   3   BSOL        : 25.00                                                
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM                              
REMARK   3  PARAMETER FILE  2  : DNA-RNA_REP.PARAM                              
REMARK   3  PARAMETER FILE  3  : WATER_REP.PARAM                                
REMARK   3  PARAMETER FILE  4  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : PROTEIN.TOP                                    
REMARK   3  TOPOLOGY FILE  2   : DNA-RNA.TOP                                    
REMARK   3  TOPOLOGY FILE  3   : WATER.TOP                                      
REMARK   3  TOPOLOGY FILE  4   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1GTW COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 22-JAN-02.                  
REMARK 100 THE PDBE ID CODE IS EBI-9253.                                        
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 11-DEC-98                          
REMARK 200  TEMPERATURE           (KELVIN) : 110.0                              
REMARK 200  PH                             : 6.50                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SPRING-8                           
REMARK 200  BEAMLINE                       : BL44B2                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.6                                
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE RAXIS IV               
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU                             
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 44759                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.700                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 20.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 95.8                               
REMARK 200  DATA REDUNDANCY                : 4.008                              
REMARK 200  R MERGE                    (I) : 0.05200                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 21.0506                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.70                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.76                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 82.9                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 2.43                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.43000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.343                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: X-PLOR                                                
REMARK 200 STARTING MODEL: PDB ENTRY 1H8A                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 66.0                                      
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.70                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M POTASSIUM CHLORIDE,                
REMARK 280  0.1 M MAGNESIUM ACETATE, 10.0% W/V PEG 8000,                        
REMARK 280  0.05 M SODIUM CACODYLATE CACODYLATE BUFFER PH 6.5,                  
REMARK 280  PROTEIN-DNA COMPLEX SOLUTION CONTAINS 0.01 M DTT AND                
REMARK 280  0.005 M NAN3, PROTEIN:DNA RATIO IS 1:1.2                            
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -X,Y,-Z+1/2                                             
REMARK 290       4555   X,-Y,-Z                                                 
REMARK 290       5555   X+1/2,Y+1/2,Z                                           
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2                                     
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2                                     
REMARK 290       8555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       37.17450            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       37.17450            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       50.46900            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       56.26650            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       50.46900            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       56.26650            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       37.17450            
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       50.46900            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       56.26650            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       37.17450            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       50.46900            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       56.26650            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 300                                                                      
REMARK 300 DETAILS: DIMER OF TETRAMER CONTAINING TWO PROTEIN                    
REMARK 300 CHAINS AND TWO DNA CHAINS                                            
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: OCTAMERIC                         
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: OCTAMERIC                  
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 13900 ANGSTROM**2                         
REMARK 350 SURFACE AREA OF THE COMPLEX: 27120 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -94.5 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   2  0.000000 -1.000000  0.000000      112.53300            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000      148.69800            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 6050 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 14460 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -44.8 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.000000           
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375      HOH A2045   LIES ON A SPECIAL POSITION.                         
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     VAL A   259                                                      
REMARK 465     LYS A   260                                                      
REMARK 465     SER A   261                                                      
REMARK 465     LYS A   262                                                      
REMARK 465     ALA A   263                                                      
REMARK 465     LYS A   264                                                      
REMARK 465     LYS A   265                                                      
REMARK 465     THR A   266                                                      
REMARK 465     VAL A   267                                                      
REMARK 465     LEU A   334                                                      
REMARK 465     PRO A   335                                                      
REMARK 465     GLU A   336                                                      
REMARK 465     VAL B   259                                                      
REMARK 465     LYS B   260                                                      
REMARK 465     SER B   261                                                      
REMARK 465     LYS B   262                                                      
REMARK 465     ALA B   263                                                      
REMARK 465     LYS B   264                                                      
REMARK 465     LYS B   265                                                      
REMARK 465     THR B   266                                                      
REMARK 465     VAL B   267                                                      
REMARK 465     GLU B   336                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLN A 333    CA   C    O    CB   CG   CD   OE1  NE2              
REMARK 470     PRO B 335    CA   C    O    CB   CG   CD                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500     DG D 103   O4' -  C1' -  N9  ANGL. DEV. =   2.0 DEGREES          
REMARK 500     DG D 110   O4' -  C1' -  N9  ANGL. DEV. =   2.5 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    SER B 271      156.88    -47.62                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1GU4   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF C/EBPBETA BZIP                                 
REMARK 900  DIMERIC   BOUND TO A HIGH AFFINITY DNA FRAGMENT                     
REMARK 900 RELATED ID: 1GU5   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF C/EBPBETA BZIP                                 
REMARK 900  DIMERIC   BOUND TO A HIGH AFFINITY                                  
REMARK 900  DNA FRAGMENT FROM THE MIM-1 PROMOTER                                
REMARK 900 RELATED ID: 1H88   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA                            
REMARK 900  COMPLEX1                                                            
REMARK 900 RELATED ID: 1H89   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA                            
REMARK 900  COMPLEX2                                                            
REMARK 900 RELATED ID: 1H8A   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA                            
REMARK 900  COMPLEX3                                                            
REMARK 900 RELATED ID: 1HJB   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA                           
REMARK 900  RUNT DOMAIN AND C/EBPBETA BZIP DIMERIC                              
REMARK 900  BOUND TO A DNA FRAGMENT FROM THE CSF-1R                             
REMARK 900   PROMOTER                                                           
DBREF  1GTW A  259   336  UNP    P17676   CEBPB_HUMAN    259    336             
DBREF  1GTW B  259   336  UNP    P17676   CEBPB_HUMAN    259    336             
DBREF  1GTW C    1    16  PDB    1GTW     1GTW             1     16             
DBREF  1GTW D  101   116  PDB    1GTW     1GTW           101    116             
SEQRES   1 C   16   DA  DA  DT  DG  DT  DG  DG  DC  DG  DC  DA  DA  DT          
SEQRES   2 C   16   DC  DC  DT                                                  
SEQRES   1 D   16   DT  DA  DG  DG  DA  DT  DT  DG  DC  DG  DC  DC  DA          
SEQRES   2 D   16   DC  DA  DT                                                  
SEQRES   1 A   78  VAL LYS SER LYS ALA LYS LYS THR VAL ASP LYS HIS SER          
SEQRES   2 A   78  ASP GLU TYR LYS ILE ARG ARG GLU ARG ASN ASN ILE ALA          
SEQRES   3 A   78  VAL ARG LYS SER ARG ASP LYS ALA LYS MET ARG ASN LEU          
SEQRES   4 A   78  GLU THR GLN HIS LYS VAL LEU GLU LEU THR ALA GLU ASN          
SEQRES   5 A   78  GLU ARG LEU GLN LYS LYS VAL GLU GLN LEU SER ARG GLU          
SEQRES   6 A   78  LEU SER THR LEU ARG ASN LEU PHE LYS GLN LEU PRO GLU          
SEQRES   1 B   78  VAL LYS SER LYS ALA LYS LYS THR VAL ASP LYS HIS SER          
SEQRES   2 B   78  ASP GLU TYR LYS ILE ARG ARG GLU ARG ASN ASN ILE ALA          
SEQRES   3 B   78  VAL ARG LYS SER ARG ASP LYS ALA LYS MET ARG ASN LEU          
SEQRES   4 B   78  GLU THR GLN HIS LYS VAL LEU GLU LEU THR ALA GLU ASN          
SEQRES   5 B   78  GLU ARG LEU GLN LYS LYS VAL GLU GLN LEU SER ARG GLU          
SEQRES   6 B   78  LEU SER THR LEU ARG ASN LEU PHE LYS GLN LEU PRO GLU          
FORMUL   5  HOH   *316(H2 O)                                                    
HELIX    1   1 SER A  271  ASN A  329  1                                  59    
HELIX    2   2 SER B  271  GLN B  333  1                                  63    
CRYST1  100.938  112.533   74.349  90.00  90.00  90.00 C 2 2 21     16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009907  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.008886  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.013450        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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