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Database: PDB
Entry: 1GU5
LinkDB: 1GU5
Original site: 1GU5 
HEADER    TRANSCRIPTION/DNA                       24-JAN-02   1GU5              
TITLE     CRYSTAL STRUCTURE OF C/EBPBETA BZIP HOMODIMER BOUND TO A DNA FRAGMENT 
TITLE    2 FROM THE MIM-1 PROMOTER                                              
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CAAT/ENHANCER BINDING PROTEIN BETA;                        
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 FRAGMENT: BZIP DOMAIN, RESIDUES 259-336;                             
COMPND   5 SYNONYM: C/EBP BETA, NUCLEAR FACTOR NF-IL6, TRANSCRIPTION FACTOR 5;  
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: 5'-D(*TP*TP*GP*TP*GP*TP*TP*GP*GP*CP* CP*AP*AP*TP*CP*A)-3'; 
COMPND   9 CHAIN: C;                                                            
COMPND  10 ENGINEERED: YES;                                                     
COMPND  11 MOL_ID: 3;                                                           
COMPND  12 MOLECULE: 5'-D(*AP*TP*GP*AP*TP*TP*GP*GP*CP*CP* AP*AP*CP*AP*CP*A)-3'; 
COMPND  13 CHAIN: D;                                                            
COMPND  14 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PAR2156;                                  
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 SYNTHETIC: YES;                                                      
SOURCE  12 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT;                            
SOURCE  13 ORGANISM_TAXID: 32630;                                               
SOURCE  14 MOL_ID: 3;                                                           
SOURCE  15 SYNTHETIC: YES;                                                      
SOURCE  16 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT;                            
SOURCE  17 ORGANISM_TAXID: 32630                                                
KEYWDS    TRANSCRIPTION/DNA, PROTEIN-DNA COMPLEX, TRANSCRIPTION FACTOR, BZIP,   
KEYWDS   2 C/EBP, TRANSCRIPTION-DNA COMPLEX                                     
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    T.H.TAHIROV,K.OGATA                                                   
REVDAT   3   29-MAR-17 1GU5    1       TITLE  SOURCE REMARK                     
REVDAT   2   24-FEB-09 1GU5    1       VERSN                                    
REVDAT   1   26-JUN-03 1GU5    0                                                
JRNL        AUTH   T.H.TAHIROV,T.INOUE-BUNGO,K.SATO,M.SASAKI,K.OGATA            
JRNL        TITL   STRUCTURAL BASIS FOR FLEXIBLE BASE RECOGNITION BY C/EBPBETA  
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   T.H.TAHIROV,K.SATO,E.ICHIKAWA-IWATA,M.SASAKI,T.INOUE-BUNGO,  
REMARK   1  AUTH 2 M.SHIINA,K.KIMURA,S.TAKATA,A.FUJIKAWA,H.MORII,T.KUMASAKA,    
REMARK   1  AUTH 3 M.YAMAMOTO,S.ISHII,K.OGATA                                   
REMARK   1  TITL   MECHANISM OF C-MYB-C/EBPBETA COOPERATION FROM SEPARATED      
REMARK   1  TITL 2 SITES ON A PROMOTER                                          
REMARK   1  REF    CELL (CAMBRIDGE,MASS.)        V. 108    57 2002              
REMARK   1  REFN                   ISSN 0092-8674                               
REMARK   1  PMID   11792321                                                     
REMARK   1  DOI    10.1016/S0092-8674(01)00636-5                                
REMARK   1 REFERENCE 2                                                          
REMARK   1  AUTH   T.H.TAHIROV,T.INOUE-BUNGO,M.SASAKI,A.FUJIKAWA,K.KIMURA,      
REMARK   1  AUTH 2 K.SATO,S.ADACHI,K.KAMIYAOGATA                                
REMARK   1  TITL   CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSIS OF THE        
REMARK   1  TITL 2 C/EBPBETA C-TERMINAL REGION IN COMPLEX WITH DNA              
REMARK   1  REF    ACTA CRYSTALLOGR.,SECT.D      V.  57   854 2001              
REMARK   1  REFN                   ISSN 0907-4449                               
REMARK   1  PMID   11375506                                                     
REMARK   1  DOI    10.1107/S0907444901003912                                    
REMARK   1 REFERENCE 3                                                          
REMARK   1  AUTH   T.H.TAHIROV,T.INOUE-BUNGO,H.MORII,A.FUJIKAWA,M.SASAKI,       
REMARK   1  AUTH 2 K.KIMURA,M.SHIINA,K.SATO,T.KUMASAKA,M.YAMAMOTO,S.ISHII,      
REMARK   1  AUTH 3 K.OGATA                                                      
REMARK   1  TITL   STRUCTURAL ANALYSES OF DNA RECOGNITION BY THE AML1/ RUNX-1   
REMARK   1  TITL 2 RUNT DOMAIN AND ITS ALLOSTERIC CONTROL BY CBFBETA            
REMARK   1  REF    CELL (CAMBRIDGE,MASS.)        V. 104   755 2001              
REMARK   1  REFN                   ISSN 0092-8674                               
REMARK   1  PMID   11257229                                                     
REMARK   1  DOI    10.1016/S0092-8674(01)00271-9                                
REMARK   1 REFERENCE 4                                                          
REMARK   1  AUTH   T.H.TAHIROV,T.INOUE,M.SASAKI,M.SHIINA,K.KIMURA,K.SATO,       
REMARK   1  AUTH 2 T.KUMASAKA,M.YAMAMOTO,N.KAMIYA,K.OGATA                       
REMARK   1  TITL   CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSES OF            
REMARK   1  TITL 2 QUATERNARY, TERNARY AND BINARY PROTEIN-DNA COMPLEXES WITH    
REMARK   1  TITL 3 INVOLVEMENT OF AML1/RUNX-1/CBFALPHA RUNT DOMAIN, CBFBETA AND 
REMARK   1  TITL 4 THE C/EBPBETA BZIP REGION                                    
REMARK   1  REF    ACTA CRYSTALLOGR.,SECT.D      V.  57   850 2001              
REMARK   1  REFN                   ISSN 0907-4449                               
REMARK   1  PMID   11375505                                                     
REMARK   1  DOI    10.1107/S0907444901003900                                    
REMARK   1 REFERENCE 5                                                          
REMARK   1  AUTH   T.H.TAHIROV,T.INOUE,M.SASAKI,M.SHIINA,K.KIMURA,K.SATO,       
REMARK   1  AUTH 2 T.KUMASAKA,M.YAMAMOTO,N.KAMIYA,K.OGATA                       
REMARK   1  TITL   CRYSTALS OF TERNARY PROTEIN-DNA COMPLEXES COMPOSED OF        
REMARK   1  TITL 2 DNA-BINDING DOMAINS C-MYB OR V-MYB, C/EBPALPHA OR C/EBPBETA  
REMARK   1  TITL 3 AND TOM-1A PROMOTER FRAGMENT                                 
REMARK   1  REF    ACTA CRYSTALLOGR.,SECT.D      V.  57  1655 2001              
REMARK   1  REFN                   ISSN 0907-4449                               
REMARK   1  PMID   11679735                                                     
REMARK   1  DOI    10.1107/S0907444901011982                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.10 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.0                                              
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,              
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : NULL                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.10                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 29.74                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : 734912.080                     
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL                           
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 99.2                           
REMARK   3   NUMBER OF REFLECTIONS             : 25194                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.229                           
REMARK   3   FREE R VALUE                     : 0.277                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.900                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1228                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : 0.008                           
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 6                            
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.10                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.23                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 97.40                        
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : 3848                         
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2700                       
REMARK   3   BIN FREE R VALUE                    : 0.3330                       
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : 5.30                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : 214                          
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : 0.023                        
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 1129                                    
REMARK   3   NUCLEIC ACID ATOMS       : 650                                     
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 224                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 44.00                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 48.30                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 4.88000                                              
REMARK   3    B22 (A**2) : -3.32000                                             
REMARK   3    B33 (A**2) : -1.57000                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : 0.29                            
REMARK   3   ESD FROM SIGMAA              (A) : 0.21                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : 30.0                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : 0.35                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : 0.26                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.005                           
REMARK   3   BOND ANGLES            (DEGREES) : 0.900                           
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : 14.70                           
REMARK   3   IMPROPER ANGLES        (DEGREES) : 1.050                           
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : RESTRAINED                                
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : 5.230 ; 1.500                
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : 6.250 ; 2.000                
REMARK   3   SIDE-CHAIN BOND              (A**2) : 7.350 ; 2.000                
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : 9.730 ; 2.500                
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : FLAT MODEL                                           
REMARK   3   KSOL        : 0.34                                                 
REMARK   3   BSOL        : 54.86                                                
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM                              
REMARK   3  PARAMETER FILE  2  : DNA-RNA_REP.PARAM                              
REMARK   3  PARAMETER FILE  3  : WATER_REP.PARAM                                
REMARK   3  PARAMETER FILE  4  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : PROTEIN.TOP                                    
REMARK   3  TOPOLOGY FILE  2   : DNA-RNA.TOP                                    
REMARK   3  TOPOLOGY FILE  3   : WATER.TOP                                      
REMARK   3  TOPOLOGY FILE  4   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1GU5 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 24-JAN-02.                  
REMARK 100 THE PDBE ID CODE IS EBI-9341.                                        
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 12-OCT-01                          
REMARK 200  TEMPERATURE           (KELVIN) : 90.0                               
REMARK 200  PH                             : 6.50                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SPRING-8                           
REMARK 200  BEAMLINE                       : BL45XU                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.02                               
REMARK 200  MONOCHROMATOR                  : DIAMOND                            
REMARK 200  OPTICS                         : MIRRORS                            
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE                        
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU RAXIS V                     
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 25871                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.080                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 30.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.4                               
REMARK 200  DATA REDUNDANCY                : 6.088                              
REMARK 200  R MERGE                    (I) : 0.06900                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 24.3640                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.08                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.12                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 97.3                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 4.55                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.46800                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 3.130                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: CNS                                                   
REMARK 200 STARTING MODEL: PDB ENTRY 1GTW                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 66.00                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.74                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.08 M MAGNESIUM ACETATE, 15.0% V/V      
REMARK 280  PEG 400, 0.05 M SODIUM CACODYLATE BUFFER PH 6.5, PROTEIN-DNA        
REMARK 280  COMPLEX CONCENTRATION WAS 12 MG/ML WITH ADDITION OF 0.01 M DTT,     
REMARK 280  PROTEIN:DNA RATIO WAS 1:1.2 FOR CRYOPROTECTION THE CONCENTRATION    
REMARK 280  OF PEG 400 WAS ADJUSTED TO 36% V/V, PH 6.50                         
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -X,Y,-Z+1/2                                             
REMARK 290       4555   X,-Y,-Z                                                 
REMARK 290       5555   X+1/2,Y+1/2,Z                                           
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2                                     
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2                                     
REMARK 290       8555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       37.24900            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       37.24900            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       50.79800            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       56.51850            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       50.79800            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       56.51850            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       37.24900            
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       50.79800            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       56.51850            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       37.24900            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       50.79800            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       56.51850            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: OCTAMERIC                         
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: OCTAMERIC                  
REMARK 350 SOFTWARE USED: PQS                                                   
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   2  0.000000 -1.000000  0.000000      113.03700            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000      148.99600            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375      HOH A2047  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     VAL A   259                                                      
REMARK 465     LYS A   260                                                      
REMARK 465     SER A   261                                                      
REMARK 465     LYS A   262                                                      
REMARK 465     ALA A   263                                                      
REMARK 465     LYS A   264                                                      
REMARK 465     LYS A   265                                                      
REMARK 465     THR A   266                                                      
REMARK 465     VAL A   267                                                      
REMARK 465     LEU A   334                                                      
REMARK 465     PRO A   335                                                      
REMARK 465     GLU A   336                                                      
REMARK 465     VAL B   259                                                      
REMARK 465     LYS B   260                                                      
REMARK 465     SER B   261                                                      
REMARK 465     LYS B   262                                                      
REMARK 465     ALA B   263                                                      
REMARK 465     LYS B   264                                                      
REMARK 465     LYS B   265                                                      
REMARK 465     THR B   266                                                      
REMARK 465     VAL B   267                                                      
REMARK 465     GLU B   336                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLN A 333    CA   C    O    CB   CG   CD   OE1                   
REMARK 470     GLN A 333    NE2                                                 
REMARK 470     PRO B 335    CA   C    O    CB   CG   CD                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   OP2   DG C     9     O    HOH C  2030              1.95            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS B 269       30.56    -68.87                                   
REMARK 500    SER B 271      174.78    -50.81                                   
REMARK 500    GLN B 333       11.32    -69.10                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500     DG C   9         0.05    SIDE CHAIN                              
REMARK 500     DT D 102         0.06    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1GTW   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF C/EBPBETA BZIP DIMERIC BOUND TO A DNA FRAGMENT  
REMARK 900 FROM THE TOM-1A PROMOTER                                             
REMARK 900 RELATED ID: 1GU4   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF C/EBPBETA BZIP DIMERIC BOUND TO A HIGH          
REMARK 900 AFFINITY DNA FRAGMENT                                                
REMARK 900 RELATED ID: 1H88   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA COMPLEX1                    
REMARK 900 RELATED ID: 1H89   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA COMPLEX2                    
REMARK 900 RELATED ID: 1H8A   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA COMPLEX3                    
REMARK 900 RELATED ID: 1HJB   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN AND C/EBPBETA  
REMARK 900 BZIP DIMERIC BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER        
REMARK 900 RELATED ID: 1IO4   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN-CBFBETA CORE   
REMARK 900 DOMAIN HETERODIMER AND C/EBPBETA BZIPHOMODIMER BOUND TO A DNA        
REMARK 900 FRAGMENT FROM THE CSF-1R PROMOTER                                    
DBREF  1GU5 A  259   336  UNP    P17676   P17676         259    336             
DBREF  1GU5 B  259   336  UNP    P17676   P17676         259    336             
DBREF  1GU5 C    1    16  PDB    1GU5     1GU5             1     16             
DBREF  1GU5 D  101   116  PDB    1GU5     1GU5           101    116             
SEQRES   1 A   78  VAL LYS SER LYS ALA LYS LYS THR VAL ASP LYS HIS SER          
SEQRES   2 A   78  ASP GLU TYR LYS ILE ARG ARG GLU ARG ASN ASN ILE ALA          
SEQRES   3 A   78  VAL ARG LYS SER ARG ASP LYS ALA LYS MET ARG ASN LEU          
SEQRES   4 A   78  GLU THR GLN HIS LYS VAL LEU GLU LEU THR ALA GLU ASN          
SEQRES   5 A   78  GLU ARG LEU GLN LYS LYS VAL GLU GLN LEU SER ARG GLU          
SEQRES   6 A   78  LEU SER THR LEU ARG ASN LEU PHE LYS GLN LEU PRO GLU          
SEQRES   1 B   78  VAL LYS SER LYS ALA LYS LYS THR VAL ASP LYS HIS SER          
SEQRES   2 B   78  ASP GLU TYR LYS ILE ARG ARG GLU ARG ASN ASN ILE ALA          
SEQRES   3 B   78  VAL ARG LYS SER ARG ASP LYS ALA LYS MET ARG ASN LEU          
SEQRES   4 B   78  GLU THR GLN HIS LYS VAL LEU GLU LEU THR ALA GLU ASN          
SEQRES   5 B   78  GLU ARG LEU GLN LYS LYS VAL GLU GLN LEU SER ARG GLU          
SEQRES   6 B   78  LEU SER THR LEU ARG ASN LEU PHE LYS GLN LEU PRO GLU          
SEQRES   1 C   16   DT  DT  DG  DT  DG  DT  DT  DG  DG  DC  DC  DA  DA          
SEQRES   2 C   16   DT  DC  DA                                                  
SEQRES   1 D   16   DA  DT  DG  DA  DT  DT  DG  DG  DC  DC  DA  DA  DC          
SEQRES   2 D   16   DA  DC  DA                                                  
FORMUL   5  HOH   *224(H2 O)                                                    
HELIX    1   1 SER A  271  LEU A  330  1                                  60    
HELIX    2   2 SER B  271  GLN B  333  1                                  63    
CRYST1  101.596  113.037   74.498  90.00  90.00  90.00 C 2 2 21     16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009843  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.008847  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.013423        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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