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Database: PDB
Entry: 1NKP
LinkDB: 1NKP
Original site: 1NKP 
HEADER    TRANSCRIPTION/DNA                       03-JAN-03   1NKP              
TITLE     CRYSTAL STRUCTURE OF MYC-MAX RECOGNIZING DNA                          
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: 5'-D(*CP*GP*AP*GP*TP*AP*GP*CP*AP*CP*GP*TP*GP*CP*TP*AP*CP*TP
COMPND   3 *C)-3';                                                              
COMPND   4 CHAIN: F, G, H, J;                                                   
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: MYC PROTO-ONCOGENE PROTEIN;                                
COMPND   8 CHAIN: A, D;                                                         
COMPND   9 FRAGMENT: BHLHZ REGION;                                              
COMPND  10 SYNONYM: C-MYC;                                                      
COMPND  11 ENGINEERED: YES;                                                     
COMPND  12 MOL_ID: 3;                                                           
COMPND  13 MOLECULE: MAX PROTEIN;                                               
COMPND  14 CHAIN: B, E;                                                         
COMPND  15 FRAGMENT: BHLHZ REGION;                                              
COMPND  16 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 MOL_ID: 2;                                                           
SOURCE   4 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   5 ORGANISM_COMMON: HUMAN;                                              
SOURCE   6 ORGANISM_TAXID: 9606;                                                
SOURCE   7 GENE: MYC;                                                           
SOURCE   8 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   9 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  10 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE  11 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  12 EXPRESSION_SYSTEM_VECTOR: PET;                                       
SOURCE  13 MOL_ID: 3;                                                           
SOURCE  14 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  15 ORGANISM_COMMON: HUMAN;                                              
SOURCE  16 ORGANISM_TAXID: 9606;                                                
SOURCE  17 GENE: MAX;                                                           
SOURCE  18 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  19 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  20 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE  21 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  22 EXPRESSION_SYSTEM_VECTOR: PET 3                                      
KEYWDS    TRANSCRIPTION, DNA, BHLHZ, ONCOGENE, HETERODIMER, TRANSCRIPTION-DNA   
KEYWDS   2 COMPLEX                                                              
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    S.K.NAIR,S.K.BURLEY                                                   
REVDAT   3   03-FEB-21 1NKP    1       AUTHOR JRNL   SEQADV                     
REVDAT   2   24-FEB-09 1NKP    1       VERSN                                    
REVDAT   1   04-FEB-03 1NKP    0                                                
JRNL        AUTH   S.K.NAIR,S.K.BURLEY                                          
JRNL        TITL   X-RAY STRUCTURES OF MYC-MAX AND MAD-MAX RECOGNIZING DNA:     
JRNL        TITL 2 MOLECULAR BASES OF REGULATION BY PROTO-ONCOGENIC             
JRNL        TITL 3 TRANSCRIPTION FACTORS                                        
JRNL        REF    CELL(CAMBRIDGE,MASS.)         V. 112   193 2003              
JRNL        REFN                   ISSN 0092-8674                               
JRNL        PMID   12553908                                                     
JRNL        DOI    10.1016/S0092-8674(02)01284-9                                
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.80 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS                                                  
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,              
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : ENGH & HUBER                                    
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.80                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 20.00                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 2.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL                           
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL                           
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : NULL                           
REMARK   3   NUMBER OF REFLECTIONS             : 40874                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.219                           
REMARK   3   FREE R VALUE                     : 0.263                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : NULL                            
REMARK   3   FREE R VALUE TEST SET COUNT      : 4123                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL                         
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL                         
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL                         
REMARK   3   BIN R VALUE           (WORKING SET) : NULL                         
REMARK   3   BIN FREE R VALUE                    : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL                         
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL                         
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 2785                                    
REMARK   3   NUCLEIC ACID ATOMS       : 1540                                    
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 581                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -0.67400                                             
REMARK   3    B22 (A**2) : 6.30000                                              
REMARK   3    B33 (A**2) : -5.62700                                             
REMARK   3    B12 (A**2) : -1.36300                                             
REMARK   3    B13 (A**2) : -2.83900                                             
REMARK   3    B23 (A**2) : -7.37800                                             
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL                            
REMARK   3   ESD FROM SIGMAA              (A) : NULL                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.005                           
REMARK   3   BOND ANGLES            (DEGREES) : NULL                            
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : NULL                            
REMARK   3   IMPROPER ANGLES        (DEGREES) : NULL                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : NULL                                      
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : NULL                                                 
REMARK   3   KSOL        : NULL                                                 
REMARK   3   BSOL        : NULL                                                 
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM                              
REMARK   3  PARAMETER FILE  2  : DNA-RNA_REP.PARAM                              
REMARK   3  PARAMETER FILE  3  : WATER_REP.PARAM                                
REMARK   3  PARAMETER FILE  4  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : NULL                                           
REMARK   3  TOPOLOGY FILE  2   : NULL                                           
REMARK   3  TOPOLOGY FILE  3   : NULL                                           
REMARK   3  TOPOLOGY FILE  4   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1NKP COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 07-JAN-03.                  
REMARK 100 THE DEPOSITION ID IS D_1000017956.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : NULL                               
REMARK 200  TEMPERATURE           (KELVIN) : NULL                               
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 19-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.91                               
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : NULL                               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 49228                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.800                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 20.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 4.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 95.9                               
REMARK 200  DATA REDUNDANCY                : NULL                               
REMARK 200  R MERGE                    (I) : 0.05300                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : NULL                               
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: PHASED TRANSLATION SEARCH    
REMARK 200 SOFTWARE USED: X-PLOR                                                
REMARK 200 STARTING MODEL: PDB ENTRY 1AN2                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 44.95                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.23                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 3-7% PEG 4000, 30% METHYL PENTANE        
REMARK 280  DIOL, 1MM COBALT HEXAMINE CHLORIDE, 50MM SODIUM CACODYLATE PH 6.5   
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1                              
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: F, G, A, B                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: H, J, D, E                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY D   497                                                      
REMARK 465     HIS D   498                                                      
REMARK 465     GLY D   582                                                      
REMARK 465     GLY D   583                                                      
REMARK 465     CYS D   584                                                      
REMARK 465     ASP E   702                                                      
REMARK 465     LYS E   703                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLU E 735    CG   CD   OE1  OE2                                  
REMARK 470     LYS E 736    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   OD1  ASN E   771     O    HOH E    57              2.13            
REMARK 500   NE   ARG D   519     O    HOH D   612              2.14            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN D 533       51.15    -93.92                                   
REMARK 500    LEU E 781      159.25    -46.14                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500     DC F 114         0.08    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1NKP A  900   981  UNP    P01106   MYC_HUMAN      353    434             
DBREF  1NKP D  500   581  UNP    P01106   MYC_HUMAN      353    434             
DBREF  1NKP B  202   281  UNP    P61244   MAX_HUMAN       23    102             
DBREF  1NKP E  702   781  UNP    P61244   MAX_HUMAN       23    102             
DBREF  1NKP F  101   119  PDB    1NKP     1NKP           101    119             
DBREF  1NKP G  301   319  PDB    1NKP     1NKP           301    319             
DBREF  1NKP H  601   619  PDB    1NKP     1NKP           601    619             
DBREF  1NKP J  801   819  PDB    1NKP     1NKP           801    819             
SEQADV 1NKP GLY A  897  UNP  P01106              CLONING ARTIFACT               
SEQADV 1NKP HIS A  898  UNP  P01106              CLONING ARTIFACT               
SEQADV 1NKP MET A  899  UNP  P01106              CLONING ARTIFACT               
SEQADV 1NKP GLY A  982  UNP  P01106              INSERTION                      
SEQADV 1NKP GLY A  983  UNP  P01106              INSERTION                      
SEQADV 1NKP CYS A  984  UNP  P01106              INSERTION                      
SEQADV 1NKP GLY D  497  UNP  P01106              CLONING ARTIFACT               
SEQADV 1NKP HIS D  498  UNP  P01106              CLONING ARTIFACT               
SEQADV 1NKP MET D  499  UNP  P01106              CLONING ARTIFACT               
SEQADV 1NKP GLY D  582  UNP  P01106              INSERTION                      
SEQADV 1NKP GLY D  583  UNP  P01106              INSERTION                      
SEQADV 1NKP CYS D  584  UNP  P01106              INSERTION                      
SEQADV 1NKP GLY B  282  UNP  P61244              INSERTION                      
SEQADV 1NKP GLY B  283  UNP  P61244              INSERTION                      
SEQADV 1NKP CYS B  284  UNP  P61244              INSERTION                      
SEQADV 1NKP GLY E  782  UNP  P61244              INSERTION                      
SEQADV 1NKP GLY E  783  UNP  P61244              INSERTION                      
SEQADV 1NKP CYS E  784  UNP  P61244              INSERTION                      
SEQRES   1 F   19   DC  DG  DA  DG  DT  DA  DG  DC  DA  DC  DG  DT  DG          
SEQRES   2 F   19   DC  DT  DA  DC  DT  DC                                      
SEQRES   1 G   19   DC  DG  DA  DG  DT  DA  DG  DC  DA  DC  DG  DT  DG          
SEQRES   2 G   19   DC  DT  DA  DC  DT  DC                                      
SEQRES   1 H   19   DC  DG  DA  DG  DT  DA  DG  DC  DA  DC  DG  DT  DG          
SEQRES   2 H   19   DC  DT  DA  DC  DT  DC                                      
SEQRES   1 J   19   DC  DG  DA  DG  DT  DA  DG  DC  DA  DC  DG  DT  DG          
SEQRES   2 J   19   DC  DT  DA  DC  DT  DC                                      
SEQRES   1 A   88  GLY HIS MET ASN VAL LYS ARG ARG THR HIS ASN VAL LEU          
SEQRES   2 A   88  GLU ARG GLN ARG ARG ASN GLU LEU LYS ARG SER PHE PHE          
SEQRES   3 A   88  ALA LEU ARG ASP GLN ILE PRO GLU LEU GLU ASN ASN GLU          
SEQRES   4 A   88  LYS ALA PRO LYS VAL VAL ILE LEU LYS LYS ALA THR ALA          
SEQRES   5 A   88  TYR ILE LEU SER VAL GLN ALA GLU GLU GLN LYS LEU ILE          
SEQRES   6 A   88  SER GLU GLU ASP LEU LEU ARG LYS ARG ARG GLU GLN LEU          
SEQRES   7 A   88  LYS HIS LYS LEU GLU GLN LEU GLY GLY CYS                      
SEQRES   1 B   83  ASP LYS ARG ALA HIS HIS ASN ALA LEU GLU ARG LYS ARG          
SEQRES   2 B   83  ARG ASP HIS ILE LYS ASP SER PHE HIS SER LEU ARG ASP          
SEQRES   3 B   83  SER VAL PRO SER LEU GLN GLY GLU LYS ALA SER ARG ALA          
SEQRES   4 B   83  GLN ILE LEU ASP LYS ALA THR GLU TYR ILE GLN TYR MET          
SEQRES   5 B   83  ARG ARG LYS ASN HIS THR HIS GLN GLN ASP ILE ASP ASP          
SEQRES   6 B   83  LEU LYS ARG GLN ASN ALA LEU LEU GLU GLN GLN VAL ARG          
SEQRES   7 B   83  ALA LEU GLY GLY CYS                                          
SEQRES   1 D   88  GLY HIS MET ASN VAL LYS ARG ARG THR HIS ASN VAL LEU          
SEQRES   2 D   88  GLU ARG GLN ARG ARG ASN GLU LEU LYS ARG SER PHE PHE          
SEQRES   3 D   88  ALA LEU ARG ASP GLN ILE PRO GLU LEU GLU ASN ASN GLU          
SEQRES   4 D   88  LYS ALA PRO LYS VAL VAL ILE LEU LYS LYS ALA THR ALA          
SEQRES   5 D   88  TYR ILE LEU SER VAL GLN ALA GLU GLU GLN LYS LEU ILE          
SEQRES   6 D   88  SER GLU GLU ASP LEU LEU ARG LYS ARG ARG GLU GLN LEU          
SEQRES   7 D   88  LYS HIS LYS LEU GLU GLN LEU GLY GLY CYS                      
SEQRES   1 E   83  ASP LYS ARG ALA HIS HIS ASN ALA LEU GLU ARG LYS ARG          
SEQRES   2 E   83  ARG ASP HIS ILE LYS ASP SER PHE HIS SER LEU ARG ASP          
SEQRES   3 E   83  SER VAL PRO SER LEU GLN GLY GLU LYS ALA SER ARG ALA          
SEQRES   4 E   83  GLN ILE LEU ASP LYS ALA THR GLU TYR ILE GLN TYR MET          
SEQRES   5 E   83  ARG ARG LYS ASN HIS THR HIS GLN GLN ASP ILE ASP ASP          
SEQRES   6 E   83  LEU LYS ARG GLN ASN ALA LEU LEU GLU GLN GLN VAL ARG          
SEQRES   7 E   83  ALA LEU GLY GLY CYS                                          
FORMUL   9  HOH   *581(H2 O)                                                    
HELIX    1   1 GLY A  897  ASP A  926  1                                  30    
HELIX    2   2 GLN A  927  GLU A  932  5                                   6    
HELIX    3   3 PRO A  938  GLY A  982  1                                  45    
HELIX    4   4 ASP B  202  ASP B  227  1                                  26    
HELIX    5   5 SER B  228  GLN B  233  5                                   6    
HELIX    6   6 SER B  238  ALA B  280  1                                  43    
HELIX    7   7 MET D  499  ASP D  526  1                                  28    
HELIX    8   8 GLN D  527  GLU D  532  5                                   6    
HELIX    9   9 PRO D  538  LEU D  581  1                                  44    
HELIX   10  10 ARG E  704  ASP E  727  1                                  24    
HELIX   11  11 SER E  728  GLN E  733  5                                   6    
HELIX   12  12 SER E  738  ALA E  780  1                                  43    
CRYST1   39.244   45.128   86.484  87.91  84.61  71.50 P 1           4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.025482 -0.008526 -0.002344        0.00000                         
SCALE2      0.000000  0.023367 -0.000165        0.00000                         
SCALE3      0.000000  0.000000  0.011614        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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