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Database: PDB
Entry: 1ONV
LinkDB: 1ONV
Original site: 1ONV 
HEADER    TRANSCRIPTION                           02-MAR-03   1ONV              
TITLE     NMR STRUCTURE OF A COMPLEX CONTAINING THE TFIIF SUBUNIT               
TITLE    2 RAP74 AND THE RNAP II CTD PHOSPHATASE FCP1                           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TRANSCRIPTION INITIATION FACTOR IIF, ALPHA                 
COMPND   3 SUBUNIT;                                                             
COMPND   4 CHAIN: A;                                                            
COMPND   5 FRAGMENT: C-TERMINAL DOMAIN OF RAP74, SEQUENCE DATABASE              
COMPND   6 RESIDUES 436-517;                                                    
COMPND   7 SYNONYM: TFIIF-ALFA, TRANSCRIPTION INITIATION FACTOR RAP74;          
COMPND   8 ENGINEERED: YES;                                                     
COMPND   9 MOL_ID: 2;                                                           
COMPND  10 MOLECULE: SERINE PHOSPHATASE FCP1A;                                  
COMPND  11 CHAIN: B;                                                            
COMPND  12 FRAGMENT: C-TERMINAL DOMAIN OF FCP1, SEQUENCE DATABASE               
COMPND  13 RESIDUES 760-842;                                                    
COMPND  14 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PGEX;                                 
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: GST-2T;                                   
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  12 ORGANISM_COMMON: HUMAN;                                              
SOURCE  13 ORGANISM_TAXID: 9606;                                                
SOURCE  14 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  15 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  16 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE  17 EXPRESSION_SYSTEM_VECTOR_TYPE: PGEX;                                 
SOURCE  18 EXPRESSION_SYSTEM_PLASMID: GST-3X                                    
KEYWDS    TRANSCRIPTION FACTOR, HUMAN GENERAL TRANSCRIPTION FACTOR              
KEYWDS   2 TFIIF, RAP74, RNA POLYMERASE II CTD PHOSPHATASE, TFIIF-              
KEYWDS   3 ASSOCIATING CTD PHOSPHATASE, FCP1                                    
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    B.D.NGUYEN,K.L.ABBOTT,K.POTEMPA,M.S.KOBOR,J.ARCHAMBAULT,              
AUTHOR   2 J.GREENBLATT,P.LEGAULT,J.G.OMICHINSKI                                
REVDAT   2   24-FEB-09 1ONV    1       VERSN                                    
REVDAT   1   20-MAY-03 1ONV    0                                                
JRNL        AUTH   B.D.NGUYEN,K.L.ABBOTT,K.POTEMPA,M.S.KOBOR,                   
JRNL        AUTH 2 J.ARCHAMBAULT,J.GREENBLATT,P.LEGAULT,J.G.OMICHINSKI          
JRNL        TITL   NMR STRUCTURE OF A COMPLEX CONTAINING THE TFIIF              
JRNL        TITL 2 SUBUNIT RAP74 AND THE RNA POLYMERASE II                      
JRNL        TITL 3 CARBOXYL-TERMINAL DOMAIN PHOSPHATASE FCP1                    
JRNL        REF    PROC.NATL.ACAD.SCI.USA        V. 100  5688 2003              
JRNL        REFN                   ISSN 0027-8424                               
JRNL        PMID   12732728                                                     
JRNL        DOI    10.1073/PNAS.1031524100                                      
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS MODIFIED CNS WITH CONFORMATIONAL DATABASE        
REMARK   3                 POTENTIAL                                            
REMARK   3   AUTHORS     : KAY AND CHOY, CLORES                                 
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: THE STRUCTURES WERE DETERMINED BASED      
REMARK   3  ON A TOTAL OF 1267 RESTRAINTS, 1131 NOE-DERIVED DISTANCE            
REMARK   3  RESTRAINTS (INCLUDING 58 INTERMOLECULAR NOE-DERIVED DISTANCE        
REMARK   3  RESTRAINTS) AND 136 DIHEDRAL ANGLE RESTRAINTS                       
REMARK   4                                                                      
REMARK   4 1ONV COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 10-MAR-03.                  
REMARK 100 THE RCSB ID CODE IS RCSB018482.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 300                                
REMARK 210  PH                             : 6.5                                
REMARK 210  IONIC STRENGTH                 : 20MM SODIUM PHOSPHATE BUFFER       
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1MM CTERRAP74/CTERFCP1, 20MM       
REMARK 210                                   SODIUM PHOSPHATE, AND 1MM          
REMARK 210                                   EDTA; 1MM U-15N CTERRAP74/         
REMARK 210                                   CTERFCP1, 20MM SODIUM              
REMARK 210                                   PHOSPHATE, AND 1MM EDTA; 1MM U     
REMARK 210                                   -15N,13C CTERRAP74/CTERFCP1,       
REMARK 210                                   20MM SODIUM PHOSPHATE, AND 1MM     
REMARK 210                                   EDTA; 1MM CTERRAP74/U-15N          
REMARK 210                                   CTERFCP1, 20MM SODIUM              
REMARK 210                                   PHOSPHATE, AND 1MM EDTA; 1MM       
REMARK 210                                   CTERRAP74/U-15N,13C CTERFCP1,      
REMARK 210                                   20MM SODIUM PHOSPHATE, AND 1MM     
REMARK 210                                   EDTA; 1MM U-15N,C13 CTERRAP74/     
REMARK 210                                   CTERFCP1, 20MM SODIUM              
REMARK 210                                   PHOSPHATE, AND 1MM EDTA;; 1MM      
REMARK 210                                   CTERRAP74/U-15N,13C CTERFCP1,      
REMARK 210                                   20MM SODIUM PHOSPHATE, AND 1MM     
REMARK 210                                   EDTA                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D NOESY, 3D_15N-SEPARATED_        
REMARK 210                                   NOESY, HNCO, (HB)CBCA(CO)NNH,      
REMARK 210                                   HNCACB, H(CCO)NNH-TOCSY, C(CO)     
REMARK 210                                   NNH-TOCSY, 3D_13C-SEPARATED_       
REMARK 210                                   NOESY, 2D 13C-FILTERD/EDITED       
REMARK 210                                   NOESY, 3D 13C-FILTERD/EDITED       
REMARK 210                                   NOESY, 3D 15N/13C SEPARATED        
REMARK 210                                   NOESY                              
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 600 MHZ, 800 MHZ          
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : CNS 1.0, NMRPIPE, PIPP,            
REMARK 210                                   NMRVIEW 5.0                        
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 70                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH ACCEPTABLE         
REMARK 210                                   COVALENT GEOMETRY,STRUCTURES       
REMARK 210                                   WITH THE LEAST RESTRAINT           
REMARK 210                                   VIOLATIONS,STRUCTURES WITH THE     
REMARK 210                                   LOWEST ENERGY                      
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR      
REMARK 210  SPECTROSCOPY                                                        
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER;                   
REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                            
REMARK 465   MODELS 1-20                                                        
REMARK 465     RES C SSSEQI                                                     
REMARK 465     SER A   436                                                      
REMARK 465     THR A   437                                                      
REMARK 465     PRO A   438                                                      
REMARK 465     GLN A   439                                                      
REMARK 465     PRO A   440                                                      
REMARK 465     PRO A   441                                                      
REMARK 465     SER A   442                                                      
REMARK 465     GLY A   443                                                      
REMARK 465     LYS A   444                                                      
REMARK 465     THR A   445                                                      
REMARK 465     THR A   446                                                      
REMARK 465     PRO A   447                                                      
REMARK 465     ASN A   448                                                      
REMARK 465     SER A   449                                                      
REMARK 465     GLY A   450                                                      
REMARK 465     PRO B   879                                                      
REMARK 465     GLU B   880                                                      
REMARK 465     GLU B   881                                                      
REMARK 465     GLN B   882                                                      
REMARK 465     GLU B   883                                                      
REMARK 465     GLU B   884                                                      
REMARK 465     GLU B   885                                                      
REMARK 465     PRO B   886                                                      
REMARK 465     GLN B   887                                                      
REMARK 465     PRO B   888                                                      
REMARK 465     ARG B   889                                                      
REMARK 465     LYS B   890                                                      
REMARK 465     PRO B   891                                                      
REMARK 465     GLY B   892                                                      
REMARK 465     THR B   893                                                      
REMARK 465     ARG B   894                                                      
REMARK 465     ARG B   895                                                      
REMARK 465     GLU B   896                                                      
REMARK 465     ARG B   897                                                      
REMARK 465     THR B   898                                                      
REMARK 465     LEU B   899                                                      
REMARK 465     GLY B   900                                                      
REMARK 465     ALA B   901                                                      
REMARK 465     PRO B   902                                                      
REMARK 465     ALA B   903                                                      
REMARK 465     SER B   904                                                      
REMARK 465     SER B   905                                                      
REMARK 465     GLU B   906                                                      
REMARK 465     ARG B   907                                                      
REMARK 465     SER B   908                                                      
REMARK 465     ALA B   909                                                      
REMARK 465     ALA B   910                                                      
REMARK 465     GLY B   911                                                      
REMARK 465     GLY B   912                                                      
REMARK 465     ARG B   913                                                      
REMARK 465     GLY B   914                                                      
REMARK 465     PRO B   915                                                      
REMARK 465     ARG B   916                                                      
REMARK 465     GLY B   917                                                      
REMARK 465     HIS B   918                                                      
REMARK 465     LYS B   919                                                      
REMARK 465     ARG B   920                                                      
REMARK 465     LYS B   921                                                      
REMARK 465     LEU B   922                                                      
REMARK 465     ASN B   923                                                      
REMARK 465     GLU B   924                                                      
REMARK 465     GLU B   925                                                      
REMARK 465     ASP B   926                                                      
REMARK 465     ALA B   927                                                      
REMARK 465     ALA B   928                                                      
REMARK 465     SER B   929                                                      
REMARK 465     GLU B   930                                                      
REMARK 465     SER B   931                                                      
REMARK 465     SER B   932                                                      
REMARK 465     ARG B   933                                                      
REMARK 465     GLU B   934                                                      
REMARK 465     SER B   935                                                      
REMARK 465     SER B   936                                                      
REMARK 465     ASN B   937                                                      
REMARK 465     GLU B   938                                                      
REMARK 465     ASP B   939                                                      
REMARK 465     GLU B   940                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 THR A 479       33.97   -169.75                                   
REMARK 500  1 LYS A 481      -44.44   -130.78                                   
REMARK 500  1 PRO A 502     -168.39    -75.83                                   
REMARK 500  1 SER B 944      -66.03   -165.70                                   
REMARK 500  2 THR A 479       34.27   -170.62                                   
REMARK 500  2 LYS A 481       29.73   -162.57                                   
REMARK 500  2 SER B 944      -52.92   -172.20                                   
REMARK 500  3 PHE A 477      -72.13    -87.78                                   
REMARK 500  3 LYS A 481     -160.15     53.42                                   
REMARK 500  3 THR A 482      -59.95     72.29                                   
REMARK 500  3 ASN A 508      -78.62     63.25                                   
REMARK 500  3 ASP A 509       29.24   -160.12                                   
REMARK 500  3 SER B 944      -64.77   -176.88                                   
REMARK 500  4 PHE A 477       47.74   -157.15                                   
REMARK 500  4 LYS A 481       83.01    -66.00                                   
REMARK 500  4 SER B 944      -58.24   -160.13                                   
REMARK 500  5 THR A 479       87.07   -158.18                                   
REMARK 500  6 THR A 479       93.18    -55.68                                   
REMARK 500  6 SER B 943       34.89   -170.34                                   
REMARK 500  6 SER B 944      -75.57   -124.50                                   
REMARK 500  7 LYS A 481       84.50    -61.88                                   
REMARK 500  7 SER B 944      -56.22   -162.37                                   
REMARK 500  8 THR A 479       90.48    -56.37                                   
REMARK 500  8 LYS A 480      -76.64   -132.89                                   
REMARK 500  8 SER B 943      -50.06   -150.80                                   
REMARK 500  8 SER B 944      -70.00     67.39                                   
REMARK 500  9 GLN A 478      -52.17   -176.39                                   
REMARK 500  9 LYS A 480      -69.68    -97.71                                   
REMARK 500  9 LYS A 481       48.00   -176.32                                   
REMARK 500  9 PRO A 502     -168.59    -77.06                                   
REMARK 500  9 SER B 944      -63.88   -133.09                                   
REMARK 500 10 PHE A 477       66.91   -151.75                                   
REMARK 500 10 LYS A 480     -173.93    -65.77                                   
REMARK 500 10 SER B 943       81.46     59.10                                   
REMARK 500 10 SER B 944      -52.12   -160.41                                   
REMARK 500 11 THR A 479       27.11   -157.67                                   
REMARK 500 11 LYS A 481      100.49   -175.77                                   
REMARK 500 11 THR A 482      -44.66   -155.08                                   
REMARK 500 11 PRO A 502     -167.71    -73.87                                   
REMARK 500 11 SER B 943     -176.88     59.45                                   
REMARK 500 11 SER B 944      -65.33     69.44                                   
REMARK 500 12 THR A 479       47.09   -175.67                                   
REMARK 500 12 LYS A 481       66.20    -69.68                                   
REMARK 500 12 SER B 942       77.84     58.91                                   
REMARK 500 12 SER B 943       37.03   -174.44                                   
REMARK 500 13 LYS A 480      -76.72   -102.21                                   
REMARK 500 13 LYS A 481       28.67   -153.95                                   
REMARK 500 14 GLN A 478      -61.97   -128.18                                   
REMARK 500 15 THR A 482       23.61   -150.63                                   
REMARK 500 15 SER B 942       91.02     59.28                                   
REMARK 500 15 SER B 944      -76.55   -129.79                                   
REMARK 500 16 PHE A 477       42.26   -148.00                                   
REMARK 500 16 SER B 942      107.27     59.27                                   
REMARK 500 16 SER B 944      -74.86   -120.49                                   
REMARK 500 17 THR A 479       62.56   -171.07                                   
REMARK 500 17 LYS A 480      -74.58    -80.08                                   
REMARK 500 17 LYS A 481       28.28   -154.39                                   
REMARK 500 18 GLN A 478      -59.46   -177.24                                   
REMARK 500 18 LYS A 481       82.16    -63.60                                   
REMARK 500 19 GLN A 478      -46.24   -134.98                                   
REMARK 500 19 LYS A 480      -69.56    -95.58                                   
REMARK 500 19 LYS A 481       37.94   -177.49                                   
REMARK 500 20 THR A 479       39.82    178.89                                   
REMARK 500 20 LYS A 480      -76.20    -75.46                                   
REMARK 500 20 PRO A 502     -168.31    -75.66                                   
REMARK 500 20 SER B 944      -58.87   -150.44                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1ONV A  436   517  UNP    P35269   T2FA_HUMAN     436    517             
DBREF  1ONV B  879   961  UNP    Q9Y5B0   CTDP1_HUMAN    760    842             
SEQRES   1 A   82  SER THR PRO GLN PRO PRO SER GLY LYS THR THR PRO ASN          
SEQRES   2 A   82  SER GLY ASP VAL GLN VAL THR GLU ASP ALA VAL ARG ARG          
SEQRES   3 A   82  TYR LEU THR ARG LYS PRO MET THR THR LYS ASP LEU LEU          
SEQRES   4 A   82  LYS LYS PHE GLN THR LYS LYS THR GLY LEU SER SER GLU          
SEQRES   5 A   82  GLN THR VAL ASN VAL LEU ALA GLN ILE LEU LYS ARG LEU          
SEQRES   6 A   82  ASN PRO GLU ARG LYS MET ILE ASN ASP LYS MET HIS PHE          
SEQRES   7 A   82  SER LEU LYS GLU                                              
SEQRES   1 B   83  PRO GLU GLU GLN GLU GLU GLU PRO GLN PRO ARG LYS PRO          
SEQRES   2 B   83  GLY THR ARG ARG GLU ARG THR LEU GLY ALA PRO ALA SER          
SEQRES   3 B   83  SER GLU ARG SER ALA ALA GLY GLY ARG GLY PRO ARG GLY          
SEQRES   4 B   83  HIS LYS ARG LYS LEU ASN GLU GLU ASP ALA ALA SER GLU          
SEQRES   5 B   83  SER SER ARG GLU SER SER ASN GLU ASP GLU GLY SER SER          
SEQRES   6 B   83  SER GLU ALA ASP GLU MET ALA LYS ALA LEU GLU ALA GLU          
SEQRES   7 B   83  LEU ASN ASP LEU MET                                          
HELIX    1   1 THR A  455  LYS A  466  1                                  12    
HELIX    2   2 THR A  469  PHE A  477  1                                   9    
HELIX    3   3 SER A  485  ASN A  501  1                                  17    
HELIX    4   4 SER B  944  ASN B  958  1                                  15    
SHEET    1   A 2 GLU A 503  MET A 506  0                                        
SHEET    2   A 2 MET A 511  SER A 514 -1  O  HIS A 512   N  LYS A 505           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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