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Database: PDB
Entry: 1OTR
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Original site: 1OTR 
HEADER    CELL CYCLE                              22-MAR-03   1OTR              
TITLE     SOLUTION STRUCTURE OF A CUE-UBIQUITIN COMPLEX                         
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN CUE2;                                              
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: AMINO-TERMINAL CUE DOMAIN, RESIDUES 6-54;                  
COMPND   5 SYNONYM: HYPOTHETICAL 50.9 KD PROTEIN IN BUD2-MIF2                   
COMPND   6 INTERGENIC REGION; HYPOTHETICAL PROTEIN YKL090W;                     
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MOL_ID: 2;                                                           
COMPND   9 MOLECULE: UBIQUITIN;                                                 
COMPND  10 CHAIN: B;                                                            
COMPND  11 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE   3 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE   4 ORGANISM_TAXID: 4932;                                                
SOURCE   5 GENE: CUE2;                                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PMCSG;                                    
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  13 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  14 ORGANISM_TAXID: 4932;                                                
SOURCE  15 GENE: UBI1;                                                          
SOURCE  16 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  17 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  18 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE  19 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  20 EXPRESSION_SYSTEM_PLASMID: PET-3A                                    
KEYWDS    PROTEIN-PROTEIN COMPLEX, CELL CYCLE                                   
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    R.S.KANG,C.M.DANIELS,W.J.SALERNO,I.RADHAKRISHNAN                      
REVDAT   2   24-FEB-09 1OTR    1       VERSN                                    
REVDAT   1   24-JUN-03 1OTR    0                                                
JRNL        AUTH   R.S.KANG,C.M.DANIELS,S.A.FRANCIS,S.C.SHIH,                   
JRNL        AUTH 2 W.J.SALERNO,L.HICKE,I.RADHAKRISHNAN                          
JRNL        TITL   SOLUTION STRUCTURE OF A CUE-UBIQUITIN COMPLEX                
JRNL        TITL 2 REVEALS A CONSERVED MODE OF UBIQUITIN BINDING                
JRNL        REF    CELL(CAMBRIDGE,MASS.)         V. 113   621 2003              
JRNL        REFN                   ISSN 0092-8674                               
JRNL        PMID   12787503                                                     
JRNL        DOI    10.1016/S0092-8674(03)00362-3                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : ARIA 1.2, CNS 1.1                                    
REMARK   3   AUTHORS     : LINGE AND NILGES (ARIA), BRUNGER ET AL. (CNS)        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: THE STRUCTURES ARE BASED ON A TOTAL       
REMARK   3  OF 3328 DISTANCE RESTRAINTS, INCLUDING 3238 NOE-DERIVED             
REMARK   3  DISTANCE RESTRAINTS [2560 UNAMBIGUOUS AND 678 AMBIGUOUS             
REMARK   3  RESTRAINTS], 90 HYDROGEN BONDING DISTANCE RESTRAINTS, AND 150       
REMARK   3  TORSION ANGLE RESTRAINTS.                                           
REMARK   4                                                                      
REMARK   4 1OTR COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 25-MAR-03.                  
REMARK 100 THE RCSB ID CODE IS RCSB018659.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 20 MM SODIUM PHOSPHATE, PH         
REMARK 210                                   7.0, 0.2% NAN3                     
REMARK 210  PRESSURE                       : 1 ATM                              
REMARK 210  SAMPLE CONTENTS                : 1 MM U-15N,13C CUE2 + 1 MM         
REMARK 210                                   UBIQUITIN; 20 MM SODIUM            
REMARK 210                                   PHOSPHATE BUFFER, PH 7.0, 0.2%     
REMARK 210                                   NAN3; 1 MM U-15N,13C CUE2 + 1      
REMARK 210                                   MM UBIQUITIN; 20 MM SODIUM         
REMARK 210                                   PHOSPHATE BUFFER, PH 7.0, 0.2%     
REMARK 210                                   NAN3; 1 MM CUE2 + 1 MM U-15N,      
REMARK 210                                   13C UBIQUITIN; 20 MM SODIUM        
REMARK 210                                   PHOSPHATE BUFFER, PH 7.0, 0.2%     
REMARK 210                                   NAN3; 1 MM CUE2 + 1 MM U-15N,      
REMARK 210                                   13C UBIQUITIN; 20 MM SODIUM        
REMARK 210                                   PHOSPHATE BUFFER, PH 7.0, 0.2%     
REMARK 210                                   NAN3                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210  13C-SEPARATED_NOESY, 3D_13C-FILTERED,_13C-EDITED_NOESY, 2D_         
REMARK 210  DOUBLE_HALF-FILTERED_NOESY                                          
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ                            
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : VNMR 6.1B, FELIX 98.0 AND          
REMARK 210                                   2000, CNS 1.1, ARIA 1.2            
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS AND         
REMARK 210                                   SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 80                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : THE SUBMITTED CONFORMER            
REMARK 210                                   MODELS ARE THE 20 STRUCTURES       
REMARK 210                                   WITH THE LOWEST RESTRAINT          
REMARK 210                                   ENERGIES, RESTRAINT                
REMARK 210                                   VIOLATIONS, AND RMS DEVIATIONS     
REMARK 210                                   FROM IDEAL COVALENT GEOMETRY       
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASP A   7      -39.04   -151.63                                   
REMARK 500  1 ASP A   8       39.48   -150.67                                   
REMARK 500  1 ALA A  22      -41.22   -163.53                                   
REMARK 500  1 ASN A  35       33.65    -77.74                                   
REMARK 500  1 ASN A  50       45.78    -79.87                                   
REMARK 500  1 ASP A  52      134.98   -176.67                                   
REMARK 500  1 ASP B  21     -126.88    -83.06                                   
REMARK 500  1 LYS B  48      138.94   -173.19                                   
REMARK 500  1 GLN B  62     -154.64    -76.75                                   
REMARK 500  1 ARG B  74     -141.22     59.27                                   
REMARK 500  2 ASP A   8       54.60   -174.81                                   
REMARK 500  2 ALA A  22      -44.38   -168.37                                   
REMARK 500  2 ASN A  36      -72.96     64.57                                   
REMARK 500  2 ASN A  37      -30.33   -162.09                                   
REMARK 500  2 ASP A  38      123.04    -39.27                                   
REMARK 500  2 LYS A  48       43.00    -80.83                                   
REMARK 500  2 ASN A  50       39.74    -81.05                                   
REMARK 500  2 ASP A  52      129.89     63.97                                   
REMARK 500  2 ASP B  21     -123.38    -84.29                                   
REMARK 500  2 THR B  22      141.35   -171.72                                   
REMARK 500  2 TYR B  59       31.91    -85.74                                   
REMARK 500  2 LYS B  63      140.74   -179.93                                   
REMARK 500  2 ARG B  74      133.34     64.31                                   
REMARK 500  3 ASP A   7      -47.19     75.78                                   
REMARK 500  3 ALA A  22      -39.76   -165.74                                   
REMARK 500  3 ASN A  35       38.69    -81.23                                   
REMARK 500  3 LYS A  48       39.77    -78.93                                   
REMARK 500  3 ASN A  50       41.60    -90.07                                   
REMARK 500  3 ASP A  51      133.95   -176.15                                   
REMARK 500  3 ASP A  52      132.94   -171.09                                   
REMARK 500  3 LYS B  11      116.12   -164.68                                   
REMARK 500  3 SER B  20       36.60    -92.39                                   
REMARK 500  3 ASP B  21     -134.04    -80.52                                   
REMARK 500  3 THR B  22      143.70   -175.94                                   
REMARK 500  3 GLU B  34      -32.19   -140.40                                   
REMARK 500  3 ALA B  46      -34.25     69.43                                   
REMARK 500  3 LEU B  50       35.96    -82.87                                   
REMARK 500  3 GLU B  51     -167.26     51.16                                   
REMARK 500  3 ARG B  74       46.87   -173.63                                   
REMARK 500  4 ASP A   7      138.12     69.30                                   
REMARK 500  4 ASP A   8       40.03    -76.06                                   
REMARK 500  4 ALA A  22      -42.93   -166.93                                   
REMARK 500  4 ASN A  35       37.48    -77.64                                   
REMARK 500  4 ASN A  50     -133.15    -69.62                                   
REMARK 500  4 ASP A  51      -24.32     71.79                                   
REMARK 500  4 LYS A  53      -43.31   -169.57                                   
REMARK 500  4 LEU B   8      102.13    -42.60                                   
REMARK 500  4 LYS B  11      139.28   -172.88                                   
REMARK 500  4 SER B  19       43.54    -77.35                                   
REMARK 500  4 SER B  20       49.68   -168.58                                   
REMARK 500  4 LEU B  43       86.60    -69.55                                   
REMARK 500  4 LYS B  48      144.18   -173.27                                   
REMARK 500  5 ASP A   7      -30.77   -170.16                                   
REMARK 500  5 ASP A   8       43.11   -166.50                                   
REMARK 500  5 ASN A  36      -71.00     65.41                                   
REMARK 500  5 ASN A  37       32.96   -177.09                                   
REMARK 500  5 LYS A  48       40.78    -81.47                                   
REMARK 500  5 SER B  19       30.05    -76.62                                   
REMARK 500  5 ASP B  21     -113.98    -61.20                                   
REMARK 500  5 THR B  22      146.32   -170.12                                   
REMARK 500  5 LYS B  48      138.69   -173.02                                   
REMARK 500  5 ARG B  54     -140.91    -67.21                                   
REMARK 500  5 SER B  65     -151.29    -72.72                                   
REMARK 500  6 ASP A   7      -44.23   -176.35                                   
REMARK 500  6 ASP A   8       45.17   -142.24                                   
REMARK 500  6 PRO A  21       49.87    -80.36                                   
REMARK 500  6 ALA A  22      -44.15   -168.36                                   
REMARK 500  6 ASN A  35       44.21    -78.78                                   
REMARK 500  6 GLU A  49       31.77    -75.34                                   
REMARK 500  6 ASN A  50       46.72   -145.76                                   
REMARK 500  6 ASP A  51      -45.23   -169.93                                   
REMARK 500  6 ASP A  52     -133.03     58.00                                   
REMARK 500  6 THR B   7     -148.36    -77.02                                   
REMARK 500  6 LEU B   8       48.50   -108.38                                   
REMARK 500  6 THR B   9      -52.27   -160.60                                   
REMARK 500  6 ASP B  21     -124.61    -84.43                                   
REMARK 500  6 LYS B  48      143.29   -172.36                                   
REMARK 500  6 ARG B  54     -145.08    -64.67                                   
REMARK 500  6 GLN B  62     -157.80    -85.35                                   
REMARK 500  7 ASP A   7      -40.05   -172.15                                   
REMARK 500  7 ASP A   8       40.89   -176.20                                   
REMARK 500  7 ASN A  35       36.53    -83.94                                   
REMARK 500  7 ASN A  36      -74.68     64.22                                   
REMARK 500  7 ASN A  37       28.97    179.32                                   
REMARK 500  7 ASP A  52      128.00   -175.22                                   
REMARK 500  7 LYS A  53      -46.53   -177.97                                   
REMARK 500  7 THR B   7     -150.66    -70.23                                   
REMARK 500  7 LEU B   8       41.86   -105.78                                   
REMARK 500  7 THR B   9      -53.37   -150.15                                   
REMARK 500  7 ASP B  21     -126.01    -77.13                                   
REMARK 500  7 THR B  22      140.48   -174.73                                   
REMARK 500  7 GLN B  62     -141.79    -80.95                                   
REMARK 500  8 ASP A   8       45.12   -172.54                                   
REMARK 500  8 ALA A  22       32.97    -77.08                                   
REMARK 500  8 ASN A  35       35.64    -79.66                                   
REMARK 500  8 LYS A  48       34.55    -87.20                                   
REMARK 500  8 ASN A  50       48.84    -80.77                                   
REMARK 500  8 THR B   9      -55.37   -158.01                                   
REMARK 500  8 LYS B  11      115.43   -161.57                                   
REMARK 500  8 VAL B  17      131.17   -177.23                                   
REMARK 500  8 SER B  20       35.61    -80.26                                   
REMARK 500  8 ASP B  21     -122.15    -99.65                                   
REMARK 500  8 THR B  22      140.74   -172.55                                   
REMARK 500  8 ASP B  39       40.24    -83.29                                   
REMARK 500  8 GLN B  40       35.23   -162.19                                   
REMARK 500  8 GLN B  41      146.35   -171.37                                   
REMARK 500  8 ALA B  46      -64.48     69.81                                   
REMARK 500  8 TYR B  59       30.96    -82.46                                   
REMARK 500  8 LYS B  63      148.64    179.20                                   
REMARK 500  9 ASP A   7      -42.32     75.57                                   
REMARK 500  9 ASN A  35      -71.03    -61.92                                   
REMARK 500  9 ASN A  36       42.27    167.76                                   
REMARK 500  9 LEU B   8       87.59    -48.04                                   
REMARK 500  9 SER B  19       42.30    -77.68                                   
REMARK 500  9 SER B  20       38.80   -166.71                                   
REMARK 500  9 ASP B  21     -120.78   -108.03                                   
REMARK 500  9 ALA B  46      -30.64     70.89                                   
REMARK 500  9 LEU B  50       44.14    -75.45                                   
REMARK 500  9 GLU B  51     -166.59     51.67                                   
REMARK 500  9 ASP B  52       52.12   -145.40                                   
REMARK 500  9 ARG B  54     -144.00   -141.11                                   
REMARK 500  9 GLN B  62     -114.90    -66.86                                   
REMARK 500  9 ARG B  74      -86.06     57.29                                   
REMARK 500 10 ASP A   7     -143.93   -167.86                                   
REMARK 500 10 ALA A  22      -46.83   -161.75                                   
REMARK 500 10 ASN A  35       28.83    -79.36                                   
REMARK 500 10 ASN A  36      -72.62     63.12                                   
REMARK 500 10 ASN A  37       72.65    178.09                                   
REMARK 500 10 LYS A  48       43.17    -82.92                                   
REMARK 500 10 ASN A  50       48.37   -157.32                                   
REMARK 500 10 LYS B  11      117.26    177.67                                   
REMARK 500 10 SER B  19       27.56    -76.09                                   
REMARK 500 10 SER B  20       28.06   -165.10                                   
REMARK 500 10 ASP B  21     -121.71    -76.09                                   
REMARK 500 10 THR B  22      138.71   -173.77                                   
REMARK 500 10 ARG B  72     -116.26    -72.23                                   
REMARK 500 10 ARG B  74     -141.64    -67.98                                   
REMARK 500 11 ASP A   8       33.88   -166.89                                   
REMARK 500 11 ALA A  22      -44.96   -166.92                                   
REMARK 500 11 ASN A  35       32.24    -85.12                                   
REMARK 500 11 ASN A  36      -71.89     64.90                                   
REMARK 500 11 ASN A  37       33.45   -179.09                                   
REMARK 500 11 LYS A  48       41.22    -81.36                                   
REMARK 500 11 ASN A  50      -50.40   -164.22                                   
REMARK 500 11 ASP A  51     -132.65     54.69                                   
REMARK 500 11 ASP A  52      136.57     66.47                                   
REMARK 500 11 SER B  20       35.57    -97.84                                   
REMARK 500 11 ASP B  21     -126.22    -83.45                                   
REMARK 500 11 THR B  22      143.51   -172.94                                   
REMARK 500 11 LYS B  48      146.11   -173.11                                   
REMARK 500 11 ARG B  74      -45.06   -149.02                                   
REMARK 500 12 ASP A   7     -147.95   -173.91                                   
REMARK 500 12 ASP A   8       33.61    -83.20                                   
REMARK 500 12 PRO A  21       49.98    -80.31                                   
REMARK 500 12 ALA A  22      -42.88   -168.19                                   
REMARK 500 12 ASN A  35       35.93    -82.70                                   
REMARK 500 12 ASN A  36      -72.78     63.29                                   
REMARK 500 12 ASN A  37       34.40   -178.95                                   
REMARK 500 12 ASN A  50       36.72    -84.02                                   
REMARK 500 12 ASP A  51     -146.95    -78.86                                   
REMARK 500 12 LYS A  53      133.36   -175.97                                   
REMARK 500 12 ASP B  21     -121.43    -59.89                                   
REMARK 500 12 LYS B  48      137.35   -172.74                                   
REMARK 500 12 ARG B  54     -143.21    -66.23                                   
REMARK 500 13 ASP A   7      -47.27   -157.20                                   
REMARK 500 13 ASP A   8      103.99   -174.22                                   
REMARK 500 13 ALA A  22      -48.14   -165.71                                   
REMARK 500 13 ASN A  35       32.38    -83.71                                   
REMARK 500 13 ASN A  36      -72.82     64.75                                   
REMARK 500 13 ASN A  37       31.21   -177.92                                   
REMARK 500 13 LYS A  48       42.36    -82.98                                   
REMARK 500 13 ASN A  50     -144.50    -70.46                                   
REMARK 500 13 ASP A  51     -122.19     55.37                                   
REMARK 500 13 ASP A  52      120.46     63.27                                   
REMARK 500 13 SER B  20       49.86    -82.18                                   
REMARK 500 13 LYS B  33      -72.45    -68.84                                   
REMARK 500 13 ALA B  46      -32.97     70.52                                   
REMARK 500 13 GLN B  62     -155.47    -84.02                                   
REMARK 500 13 SER B  65     -152.83    -73.75                                   
REMARK 500 13 ARG B  72      -72.94    -63.02                                   
REMARK 500 13 ARG B  74     -112.40     57.85                                   
REMARK 500 14 ASP A   7      139.53     65.21                                   
REMARK 500 14 ASP A   8       41.06   -167.96                                   
REMARK 500 14 ALA A  22      -42.97   -166.98                                   
REMARK 500 14 ASN A  35       34.55    -77.29                                   
REMARK 500 14 LYS A  48       30.13    -77.18                                   
REMARK 500 14 ASN A  50       46.43    -79.81                                   
REMARK 500 14 ASP A  52       43.35    -77.62                                   
REMARK 500 14 LYS B  11      118.54   -162.13                                   
REMARK 500 14 ASP B  21     -128.82    -79.33                                   
REMARK 500 14 THR B  22      140.46   -175.03                                   
REMARK 500 14 LYS B  33      -76.66    -73.68                                   
REMARK 500 14 ASP B  39       33.89    -78.92                                   
REMARK 500 14 GLN B  40       31.60   -161.29                                   
REMARK 500 14 LYS B  48      142.48   -172.71                                   
REMARK 500 14 GLN B  62     -138.50    -74.97                                   
REMARK 500 14 ARG B  72     -105.48    -68.12                                   
REMARK 500 14 ARG B  74     -135.10    -68.34                                   
REMARK 500 15 ASP A   7     -123.87    -69.11                                   
REMARK 500 15 ALA A  22      -39.39   -165.81                                   
REMARK 500 15 ASN A  35      -76.10    -64.73                                   
REMARK 500 15 ASN A  36       36.04    171.08                                   
REMARK 500 15 ASP A  51      128.60   -177.19                                   
REMARK 500 15 ASP A  52       41.74    -83.22                                   
REMARK 500 15 LYS A  53     -120.66     57.17                                   
REMARK 500 15 SER B  20       33.07    -90.73                                   
REMARK 500 15 ASP B  21     -141.22    -83.67                                   
REMARK 500 15 GLN B  40       35.44    -79.09                                   
REMARK 500 15 ALA B  46      -31.54     68.11                                   
REMARK 500 15 ASP B  52       46.35    -76.37                                   
REMARK 500 15 SER B  65     -148.27    -80.30                                   
REMARK 500 15 ARG B  72      166.69     57.19                                   
REMARK 500 15 LEU B  73     -147.91    -74.83                                   
REMARK 500 16 PHE A  20       76.97   -113.85                                   
REMARK 500 16 ALA A  22      -43.07   -165.20                                   
REMARK 500 16 ASN A  35       40.03    -76.87                                   
REMARK 500 16 LYS A  48       41.71    -81.35                                   
REMARK 500 16 ASP A  51      132.51     65.94                                   
REMARK 500 16 SER B  19       42.20    -77.29                                   
REMARK 500 16 SER B  20       40.45   -160.44                                   
REMARK 500 16 ASP B  21     -121.56   -121.56                                   
REMARK 500 16 LYS B  33      -76.83    -73.09                                   
REMARK 500 16 GLN B  40       38.15    -89.52                                   
REMARK 500 16 ALA B  46      -65.27     68.98                                   
REMARK 500 16 ASP B  52       46.54    -77.88                                   
REMARK 500 17 ASP A   8       34.87   -155.32                                   
REMARK 500 17 ASN A  36      -63.29    172.47                                   
REMARK 500 17 ASN A  37       33.01    173.43                                   
REMARK 500 17 LYS A  48       39.54    -82.08                                   
REMARK 500 17 ASP A  51      -44.21   -163.07                                   
REMARK 500 17 SER B  19       41.52    -77.15                                   
REMARK 500 17 SER B  20       32.99   -163.53                                   
REMARK 500 17 ASP B  21     -122.35    -98.20                                   
REMARK 500 17 LYS B  48      144.56   -173.14                                   
REMARK 500 17 GLN B  62     -155.00    -85.10                                   
REMARK 500 17 SER B  65     -151.51    -69.38                                   
REMARK 500 17 ARG B  72     -134.24    -75.12                                   
REMARK 500 17 ARG B  74       47.22   -168.15                                   
REMARK 500 18 ASP A   8       41.47    -81.94                                   
REMARK 500 18 ASN A  35       36.71    -81.10                                   
REMARK 500 18 ASN A  36      -73.15     64.48                                   
REMARK 500 18 ASN A  37       36.05    177.37                                   
REMARK 500 18 LYS A  48       43.42    -83.86                                   
REMARK 500 18 ASP A  52      -53.75   -177.04                                   
REMARK 500 18 LYS A  53     -147.00    -74.99                                   
REMARK 500 18 THR B  12       76.29   -107.18                                   
REMARK 500 18 SER B  20       33.28    -93.76                                   
REMARK 500 18 ASP B  21     -123.74    -90.83                                   
REMARK 500 18 LYS B  33      -72.10    -70.72                                   
REMARK 500 18 GLN B  40       34.33    -78.55                                   
REMARK 500 18 ALA B  46      -65.81     68.66                                   
REMARK 500 19 PRO A  21       44.58    -78.36                                   
REMARK 500 19 ALA A  22       41.79   -170.60                                   
REMARK 500 19 ASN A  35      -78.26    -64.86                                   
REMARK 500 19 ASN A  36       38.00    166.96                                   
REMARK 500 19 LYS A  48       42.21    -88.11                                   
REMARK 500 19 ASP A  52      137.26   -175.75                                   
REMARK 500 19 ASP B  21     -134.73    -91.36                                   
REMARK 500 19 GLN B  62     -157.31    -79.54                                   
REMARK 500 19 ARG B  74       45.45    -79.50                                   
REMARK 500 20 ASP A   7      -33.53   -145.54                                   
REMARK 500 20 ASP A   8       39.35    -75.63                                   
REMARK 500 20 ALA A  22      -45.24   -168.45                                   
REMARK 500 20 ASN A  35       38.77    -79.27                                   
REMARK 500 20 LYS A  48       43.14    -83.24                                   
REMARK 500 20 ASN A  50       55.80   -170.69                                   
REMARK 500 20 ASP A  51     -137.80    -64.77                                   
REMARK 500 20 SER B  20       35.10    -90.31                                   
REMARK 500 20 ASP B  21     -130.78    -78.22                                   
REMARK 500 20 THR B  22      139.41   -176.93                                   
REMARK 500 20 LYS B  48      145.56   -172.55                                   
REMARK 500 20 ARG B  74     -133.78     51.87                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1OTR A    6    54  UNP    P36075   CUE2_YEAST       6     54             
DBREF  1OTR B    1    76  UNP    P61864   UBIQ_YEAST       1     76             
SEQRES   1 A   49  ASN ASP ASP HIS GLU SER LYS LEU SER ILE LEU MET ASP          
SEQRES   2 A   49  MET PHE PRO ALA ILE SER LYS SER LYS LEU GLN VAL HIS          
SEQRES   3 A   49  LEU LEU GLU ASN ASN ASN ASP LEU ASP LEU THR ILE GLY          
SEQRES   4 A   49  LEU LEU LEU LYS GLU ASN ASP ASP LYS SER                      
SEQRES   1 B   76  MET GLN ILE PHE VAL LYS THR LEU THR GLY LYS THR ILE          
SEQRES   2 B   76  THR LEU GLU VAL GLU SER SER ASP THR ILE ASP ASN VAL          
SEQRES   3 B   76  LYS SER LYS ILE GLN ASP LYS GLU GLY ILE PRO PRO ASP          
SEQRES   4 B   76  GLN GLN ARG LEU ILE PHE ALA GLY LYS GLN LEU GLU ASP          
SEQRES   5 B   76  GLY ARG THR LEU SER ASP TYR ASN ILE GLN LYS GLU SER          
SEQRES   6 B   76  THR LEU HIS LEU VAL LEU ARG LEU ARG GLY GLY                  
HELIX    1   1 HIS A    9  MET A   19  1                                  11    
HELIX    2   2 LYS A   25  GLU A   34  1                                  10    
HELIX    3   3 LEU A   39  LEU A   47  1                                   9    
HELIX    4   4 ILE B   23  GLU B   34  1                                  12    
HELIX    5   5 LEU B   56  TYR B   59  1                                   4    
SHEET    1   A 5 THR B  12  GLU B  16  0                                        
SHEET    2   A 5 GLN B   2  THR B   7 -1  N  ILE B   3   O  LEU B  15           
SHEET    3   A 5 THR B  66  LEU B  71  1  O  LEU B  67   N  PHE B   4           
SHEET    4   A 5 GLN B  41  PHE B  45 -1  N  ILE B  44   O  HIS B  68           
SHEET    5   A 5 LYS B  48  GLN B  49 -1  O  LYS B  48   N  PHE B  45           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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