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Database: PDB
Entry: 1R05
LinkDB: 1R05
Original site: 1R05 
HEADER    TRANSCRIPTION                           19-SEP-03   1R05              
TITLE     SOLUTION STRUCTURE OF MAX B-HLH-LZ                                    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: MAX PROTEIN;                                               
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: MAX B-HLH-LZ;                                               
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: MAX;                                                           
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    BASIC-HELIX-LOOP-HELIX-LEUCINEZIPPER HOMODIMER,                       
KEYWDS   2 TRANSCRIPTION                                                        
EXPDTA    SOLUTION NMR                                                          
NUMMDL    6                                                                     
AUTHOR    S.SAUV,L.TREMBLAY,P.LAVIGNE                                           
REVDAT   3   24-FEB-09 1R05    1       VERSN                                    
REVDAT   2   11-JAN-05 1R05    1       JRNL                                     
REVDAT   1   21-OCT-03 1R05    0                                                
JRNL        AUTH   S.SAUV,L.TREMBLAY,P.LAVIGNE                                  
JRNL        TITL   THE NMR SOLUTION STRUCTURE OF A MUTANT OF THE MAX            
JRNL        TITL 2 B/HLH/LZ FREE OF DNA: INSIGHTS INTO THE SPECIFIC             
JRNL        TITL 3 AND REVERSIBLE DNA BINDING MECHANISM OF DIMERIC              
JRNL        TITL 4 TRANSCRIPTION FACTORS                                        
JRNL        REF    J.MOL.BIOL.                   V. 342   813 2004              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   15342239                                                     
JRNL        DOI    10.1016/J.JMB.2004.07.058                                    
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR                                               
REMARK   3   AUTHORS     : BRUNGER, ET AL.                                      
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1R05 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 22-SEP-03.                  
REMARK 100 THE RCSB ID CODE IS RCSB020281.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 308                                
REMARK 210  PH                             : 6.8                                
REMARK 210  IONIC STRENGTH                 : 250MM KCL                          
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 25MM MAX 15N, 50MM PHOSPHATE       
REMARK 210                                   BUFFER, 250MM KCL; 25MM MAX        
REMARK 210                                   13C,15N, 50MM PHOSPHATE            
REMARK 210                                   BUFFER, 250MM KCL                  
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 15N-NOESYHSQC, 13C-NOESYHSQC,      
REMARK 210                                   13C-HCCH-TOCSY, 13C-HCCH-COSY,     
REMARK 210                                   TRIPLE RESONANCE, SUCH AS HNCA     
REMARK 210                                   AND HNCACB                         
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ                            
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NMRPIPE                            
REMARK 210   METHOD USED                   : SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : NULL                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 6                                  
REMARK 210 CONFORMERS, SELECTION CRITERIA  : NULL                               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL                
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASP A   3      -78.60    -71.54                                   
REMARK 500  1 ARG A   5      -92.65    -85.52                                   
REMARK 500  1 ALA A   6      -88.55     53.01                                   
REMARK 500  1 HIS A   7     -154.54   -119.67                                   
REMARK 500  1 HIS A   8       63.54   -115.90                                   
REMARK 500  1 ALA A  10     -169.72   -103.13                                   
REMARK 500  1 GLU A  12     -157.67    -66.35                                   
REMARK 500  1 ARG A  13     -152.18     37.95                                   
REMARK 500  1 LYS A  14     -148.98   -164.95                                   
REMARK 500  1 ARG A  15       32.21    -84.60                                   
REMARK 500  1 ILE A  19      -79.49    -53.91                                   
REMARK 500  1 LYS A  20      -42.68    -27.94                                   
REMARK 500  1 LYS A  37       90.32    -47.47                                   
REMARK 500  1 SER A  39      169.84     66.48                                   
REMARK 500  1 TYR A  50      -71.51    -65.02                                   
REMARK 500  1 GLN A  52      -74.91    -77.53                                   
REMARK 500  1 LEU A  61      -77.95    -59.27                                   
REMARK 500  1 GLN A  71      -86.04    -58.24                                   
REMARK 500  1 SER A  85      109.78     64.05                                   
REMARK 500  1 ASP B   3      -78.59    -71.58                                   
REMARK 500  1 ARG B   5      -92.79    -85.52                                   
REMARK 500  1 ALA B   6      -88.55     53.10                                   
REMARK 500  1 HIS B   7     -154.45   -119.73                                   
REMARK 500  1 HIS B   8       63.46   -115.91                                   
REMARK 500  1 ALA B  10     -169.71   -103.09                                   
REMARK 500  1 GLU B  12     -157.73    -66.32                                   
REMARK 500  1 ARG B  13     -152.04     37.98                                   
REMARK 500  1 LYS B  14     -149.07   -165.10                                   
REMARK 500  1 ARG B  15       32.19    -84.53                                   
REMARK 500  1 ILE B  19      -79.51    -53.87                                   
REMARK 500  1 LYS B  20      -42.92    -27.96                                   
REMARK 500  1 LYS B  37       90.33    -47.44                                   
REMARK 500  1 SER B  39      169.91     66.50                                   
REMARK 500  1 TYR B  50      -71.54    -65.05                                   
REMARK 500  1 GLN B  52      -74.97    -77.48                                   
REMARK 500  1 LEU B  61      -78.12    -59.13                                   
REMARK 500  1 GLN B  71      -86.03    -58.23                                   
REMARK 500  1 ASN B  72      -27.83    -39.93                                   
REMARK 500  1 SER B  85      109.60     64.05                                   
REMARK 500  2 ALA A   2     -164.87   -129.11                                   
REMARK 500  2 LYS A   4       78.87     42.78                                   
REMARK 500  2 ARG A   5       83.75    -63.99                                   
REMARK 500  2 ALA A   6     -142.07     41.29                                   
REMARK 500  2 HIS A   7      168.94     56.64                                   
REMARK 500  2 LYS A  14     -151.18     64.84                                   
REMARK 500  2 HIS A  18      -83.12    -63.38                                   
REMARK 500  2 LEU A  33      -74.15    -87.63                                   
REMARK 500  2 GLN A  34      -89.04     49.27                                   
REMARK 500  2 GLU A  36     -139.48    -68.68                                   
REMARK 500  2 ARG A  40     -110.68     58.21                                   
REMARK 500  2 GLN A  42      -43.48     65.70                                   
REMARK 500  2 TYR A  53      -71.05    -56.57                                   
REMARK 500  2 GLN A  71      -80.15    -61.07                                   
REMARK 500  2 ASN A  72      -27.77    -39.22                                   
REMARK 500  2 ALA B   2     -164.87   -129.08                                   
REMARK 500  2 LYS B   4       78.88     42.80                                   
REMARK 500  2 ARG B   5       83.73    -63.92                                   
REMARK 500  2 ALA B   6     -142.04     41.32                                   
REMARK 500  2 HIS B   7      168.93     56.57                                   
REMARK 500  2 LYS B  14     -151.17     64.81                                   
REMARK 500  2 HIS B  18      -83.10    -63.34                                   
REMARK 500  2 LEU B  33      -73.92    -87.78                                   
REMARK 500  2 GLN B  34      -88.99     49.17                                   
REMARK 500  2 GLU B  36     -139.51    -68.68                                   
REMARK 500  2 ARG B  40     -110.71     58.14                                   
REMARK 500  2 GLN B  42      -43.42     65.79                                   
REMARK 500  2 TYR B  53      -71.02    -56.63                                   
REMARK 500  2 GLN B  71      -79.95    -61.18                                   
REMARK 500  2 ASN B  72      -27.58    -39.46                                   
REMARK 500  3 ARG A   5      -72.69   -177.02                                   
REMARK 500  3 ALA A   6     -147.27     42.93                                   
REMARK 500  3 HIS A   7       63.26   -115.16                                   
REMARK 500  3 ALA A  10       56.09    175.52                                   
REMARK 500  3 LEU A  11      -85.25    -60.43                                   
REMARK 500  3 GLU A  12      -41.51    175.70                                   
REMARK 500  3 ARG A  13      -95.60     43.78                                   
REMARK 500  3 LYS A  14      -90.69    -98.87                                   
REMARK 500  3 ARG A  15       54.95   -153.48                                   
REMARK 500  3 ILE A  19      -77.53    -47.71                                   
REMARK 500  3 LYS A  20      -40.69    -28.93                                   
REMARK 500  3 GLN A  34       70.81     79.32                                   
REMARK 500  3 GLU A  36       34.77     35.15                                   
REMARK 500  3 SER A  39      -84.43    -73.88                                   
REMARK 500  3 ARG A  40      -56.91   -128.63                                   
REMARK 500  3 ILE A  51      -75.29    -62.44                                   
REMARK 500  3 GLN A  71      -81.30    -56.70                                   
REMARK 500  3 ASN A  72      -31.49    -36.21                                   
REMARK 500  3 LEU A  82      -93.50    -45.99                                   
REMARK 500  3 SER A  85       88.61   -155.28                                   
REMARK 500  3 ARG B   5      -72.72   -177.07                                   
REMARK 500  3 ALA B   6     -147.15     42.82                                   
REMARK 500  3 HIS B   7       63.19   -115.22                                   
REMARK 500  3 ALA B  10       55.81    175.40                                   
REMARK 500  3 LEU B  11      -85.23    -60.29                                   
REMARK 500  3 GLU B  12      -41.55    175.61                                   
REMARK 500  3 ARG B  13      -95.48     43.72                                   
REMARK 500  3 LYS B  14      -90.74    -98.92                                   
REMARK 500  3 ARG B  15       54.94   -153.34                                   
REMARK 500  3 ILE B  19      -77.92    -47.50                                   
REMARK 500  3 LYS B  20      -40.80    -28.59                                   
REMARK 500  3 GLN B  34       70.68     79.42                                   
REMARK 500  3 GLU B  36       34.78     35.07                                   
REMARK 500  3 SER B  39      -84.45    -74.09                                   
REMARK 500  3 ARG B  40      -57.15   -128.62                                   
REMARK 500  3 ILE B  51      -75.32    -62.36                                   
REMARK 500  3 GLN B  71      -80.95    -56.86                                   
REMARK 500  3 ASN B  72      -29.99    -37.67                                   
REMARK 500  3 LEU B  82      -93.59    -45.89                                   
REMARK 500  3 SER B  85       88.64   -155.33                                   
REMARK 500  4 ASP A   3       75.90   -164.90                                   
REMARK 500  4 LYS A   4      115.28     60.90                                   
REMARK 500  4 ARG A   5      -80.51    -55.91                                   
REMARK 500  4 HIS A   8       74.05   -153.79                                   
REMARK 500  4 ARG A  13      159.20    -45.54                                   
REMARK 500  4 LYS A  20      -52.88    -27.77                                   
REMARK 500  4 SER A  29      -76.18    -66.25                                   
REMARK 500  4 LEU A  33       -0.17     71.11                                   
REMARK 500  4 ALA A  38       98.98     44.52                                   
REMARK 500  4 SER A  39      -78.52   -112.01                                   
REMARK 500  4 ALA A  41       35.16    -93.16                                   
REMARK 500  4 MET A  54      -72.61    -45.79                                   
REMARK 500  4 ARG A  56      -73.19    -51.46                                   
REMARK 500  4 GLN A  71      -76.05    -60.48                                   
REMARK 500  4 ASN A  72      -26.56    -39.14                                   
REMARK 500  4 GLU A  83       64.60    -66.02                                   
REMARK 500  4 SER A  85       35.97   -141.00                                   
REMARK 500  4 ASP B   3       75.84   -164.87                                   
REMARK 500  4 LYS B   4      115.21     60.96                                   
REMARK 500  4 ARG B   5      -80.54    -55.89                                   
REMARK 500  4 HIS B   8       74.01   -153.80                                   
REMARK 500  4 ARG B  13      159.25    -45.45                                   
REMARK 500  4 LYS B  20      -52.77    -27.76                                   
REMARK 500  4 SER B  29      -74.78    -66.00                                   
REMARK 500  4 LEU B  33       -0.32     71.16                                   
REMARK 500  4 ALA B  38       98.83     44.97                                   
REMARK 500  4 SER B  39      -78.50   -111.94                                   
REMARK 500  4 ALA B  41       35.25    -93.27                                   
REMARK 500  4 MET B  54      -72.77    -45.84                                   
REMARK 500  4 ARG B  56      -73.21    -51.35                                   
REMARK 500  4 GLN B  71      -75.88    -60.40                                   
REMARK 500  4 ASN B  72      -27.12    -38.77                                   
REMARK 500  4 GLU B  83       64.53    -66.06                                   
REMARK 500  4 SER B  85       36.00   -141.01                                   
REMARK 500  5 ALA A   2       99.12   -175.91                                   
REMARK 500  5 ASP A   3       61.27   -112.86                                   
REMARK 500  5 HIS A   7      -58.14    177.89                                   
REMARK 500  5 ASN A   9      -73.52     60.47                                   
REMARK 500  5 ALA A  10      176.41     58.64                                   
REMARK 500  5 ARG A  13     -152.74   -131.14                                   
REMARK 500  5 ASP A  17      -59.56   -132.90                                   
REMARK 500  5 HIS A  18      -73.10    -39.41                                   
REMARK 500  5 ILE A  19      -78.84    -43.92                                   
REMARK 500  5 GLN A  34       18.36     43.40                                   
REMARK 500  5 ALA A  38     -168.22     39.77                                   
REMARK 500  5 SER A  39     -162.92    158.80                                   
REMARK 500  5 ALA A  41       71.04   -117.89                                   
REMARK 500  5 LEU A  44      -38.35    -31.56                                   
REMARK 500  5 LEU A  61      -72.28    -69.41                                   
REMARK 500  5 GLN A  71      -82.13    -47.56                                   
REMARK 500  5 ASN A  72      -26.79    -38.60                                   
REMARK 500  5 GLU A  83       80.31    -58.96                                   
REMARK 500  5 ALA B   2       99.19   -175.94                                   
REMARK 500  5 ASP B   3       61.19   -112.93                                   
REMARK 500  5 HIS B   7      -58.21    177.77                                   
REMARK 500  5 ASN B   9      -73.48     60.56                                   
REMARK 500  5 ALA B  10      176.38     58.71                                   
REMARK 500  5 ARG B  13     -152.80   -131.13                                   
REMARK 500  5 ASP B  17      -59.53   -132.99                                   
REMARK 500  5 HIS B  18      -73.19    -39.32                                   
REMARK 500  5 ILE B  19      -78.89    -43.67                                   
REMARK 500  5 VAL B  30       72.56   -118.82                                   
REMARK 500  5 GLN B  34       18.43     43.34                                   
REMARK 500  5 ALA B  38     -168.21     39.83                                   
REMARK 500  5 SER B  39     -162.94    158.77                                   
REMARK 500  5 ALA B  41       70.89   -117.63                                   
REMARK 500  5 LEU B  44      -38.33    -31.55                                   
REMARK 500  5 LEU B  61      -72.16    -69.39                                   
REMARK 500  5 GLN B  71      -81.88    -47.27                                   
REMARK 500  5 ASN B  72      -29.30    -36.70                                   
REMARK 500  5 GLU B  83       80.29    -58.88                                   
REMARK 500  6 ALA A   2       99.42    -65.80                                   
REMARK 500  6 ARG A   5       71.72   -151.56                                   
REMARK 500  6 ALA A   6     -155.51    -62.83                                   
REMARK 500  6 HIS A   8       77.66     42.52                                   
REMARK 500  6 ALA A  10      107.60   -160.79                                   
REMARK 500  6 LYS A  14     -143.61     46.51                                   
REMARK 500  6 ARG A  15       46.21   -108.07                                   
REMARK 500  6 ARG A  16       58.74   -144.21                                   
REMARK 500  6 ASP A  17      -30.29    178.85                                   
REMARK 500  6 SER A  32       -8.29     68.66                                   
REMARK 500  6 LEU A  33       16.71     54.56                                   
REMARK 500  6 GLU A  36      159.69    -35.43                                   
REMARK 500  6 LYS A  37      -16.44    171.80                                   
REMARK 500  6 ALA A  38      -93.65    -32.06                                   
REMARK 500  6 SER A  39      160.49    163.82                                   
REMARK 500  6 ARG A  40     -102.08     41.87                                   
REMARK 500  6 GLN A  42      -18.35     74.66                                   
REMARK 500  6 GLN A  52      -73.38    -80.10                                   
REMARK 500  6 ARG A  55      -71.23    -41.16                                   
REMARK 500  6 GLN A  71      -81.45    -50.55                                   
REMARK 500  6 LEU A  82       33.66   -156.50                                   
REMARK 500  6 ALA B   2       99.27    -65.53                                   
REMARK 500  6 ARG B   5       71.69   -151.43                                   
REMARK 500  6 ALA B   6     -155.55    -62.71                                   
REMARK 500  6 HIS B   8       77.69     42.46                                   
REMARK 500  6 ALA B  10      107.62   -160.72                                   
REMARK 500  6 LYS B  14     -143.67     46.46                                   
REMARK 500  6 ARG B  15       45.92   -108.00                                   
REMARK 500  6 ARG B  16       58.71   -143.86                                   
REMARK 500  6 ASP B  17      -30.27    178.88                                   
REMARK 500  6 SER B  32       -8.67     68.67                                   
REMARK 500  6 LEU B  33       16.51     54.72                                   
REMARK 500  6 GLU B  36      159.00    -33.55                                   
REMARK 500  6 LYS B  37      -16.32    171.92                                   
REMARK 500  6 ALA B  38      -93.32    -32.38                                   
REMARK 500  6 SER B  39      160.57    163.84                                   
REMARK 500  6 ARG B  40     -101.85     41.72                                   
REMARK 500  6 GLN B  42      -18.49     74.94                                   
REMARK 500  6 GLN B  52      -73.07    -80.29                                   
REMARK 500  6 ARG B  55      -71.14    -41.56                                   
REMARK 500  6 GLN B  71      -81.52    -50.21                                   
REMARK 500  6 LEU B  82       33.71   -156.63                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 ARG A   5         0.27    SIDE_CHAIN                              
REMARK 500  1 ARG A  13         0.28    SIDE_CHAIN                              
REMARK 500  1 ARG A  15         0.23    SIDE_CHAIN                              
REMARK 500  1 ARG A  16         0.10    SIDE_CHAIN                              
REMARK 500  1 ARG A  27         0.29    SIDE_CHAIN                              
REMARK 500  1 ARG A  40         0.30    SIDE_CHAIN                              
REMARK 500  1 ARG A  55         0.22    SIDE_CHAIN                              
REMARK 500  1 ARG A  56         0.19    SIDE_CHAIN                              
REMARK 500  1 ARG A  70         0.24    SIDE_CHAIN                              
REMARK 500  1 ARG A  80         0.20    SIDE_CHAIN                              
REMARK 500  1 ARG B   5         0.27    SIDE_CHAIN                              
REMARK 500  1 ARG B  13         0.28    SIDE_CHAIN                              
REMARK 500  1 ARG B  15         0.23    SIDE_CHAIN                              
REMARK 500  1 ARG B  16         0.10    SIDE_CHAIN                              
REMARK 500  1 ARG B  27         0.29    SIDE_CHAIN                              
REMARK 500  1 ARG B  40         0.30    SIDE_CHAIN                              
REMARK 500  1 ARG B  55         0.21    SIDE_CHAIN                              
REMARK 500  1 ARG B  56         0.19    SIDE_CHAIN                              
REMARK 500  1 ARG B  70         0.24    SIDE_CHAIN                              
REMARK 500  1 ARG B  80         0.20    SIDE_CHAIN                              
REMARK 500  2 ARG A   5         0.23    SIDE_CHAIN                              
REMARK 500  2 ARG A  13         0.14    SIDE_CHAIN                              
REMARK 500  2 ARG A  15         0.23    SIDE_CHAIN                              
REMARK 500  2 ARG A  16         0.28    SIDE_CHAIN                              
REMARK 500  2 ARG A  27         0.22    SIDE_CHAIN                              
REMARK 500  2 ARG A  55         0.21    SIDE_CHAIN                              
REMARK 500  2 ARG A  56         0.32    SIDE_CHAIN                              
REMARK 500  2 ARG A  70         0.27    SIDE_CHAIN                              
REMARK 500  2 ARG A  80         0.32    SIDE_CHAIN                              
REMARK 500  2 ARG B   5         0.23    SIDE_CHAIN                              
REMARK 500  2 ARG B  13         0.14    SIDE_CHAIN                              
REMARK 500  2 ARG B  15         0.23    SIDE_CHAIN                              
REMARK 500  2 ARG B  16         0.28    SIDE_CHAIN                              
REMARK 500  2 ARG B  27         0.22    SIDE_CHAIN                              
REMARK 500  2 ARG B  55         0.21    SIDE_CHAIN                              
REMARK 500  2 ARG B  56         0.32    SIDE_CHAIN                              
REMARK 500  2 ARG B  70         0.27    SIDE_CHAIN                              
REMARK 500  2 ARG B  80         0.32    SIDE_CHAIN                              
REMARK 500  3 ARG A   5         0.23    SIDE_CHAIN                              
REMARK 500  3 ARG A  15         0.28    SIDE_CHAIN                              
REMARK 500  3 ARG A  16         0.14    SIDE_CHAIN                              
REMARK 500  3 ARG A  40         0.16    SIDE_CHAIN                              
REMARK 500  3 ARG A  55         0.31    SIDE_CHAIN                              
REMARK 500  3 ARG A  56         0.23    SIDE_CHAIN                              
REMARK 500  3 ARG A  70         0.20    SIDE_CHAIN                              
REMARK 500  3 ARG A  80         0.27    SIDE_CHAIN                              
REMARK 500  3 ARG B   5         0.23    SIDE_CHAIN                              
REMARK 500  3 ARG B  15         0.28    SIDE_CHAIN                              
REMARK 500  3 ARG B  16         0.14    SIDE_CHAIN                              
REMARK 500  3 ARG B  40         0.16    SIDE_CHAIN                              
REMARK 500  3 ARG B  55         0.31    SIDE_CHAIN                              
REMARK 500  3 ARG B  56         0.23    SIDE_CHAIN                              
REMARK 500  3 ARG B  70         0.21    SIDE_CHAIN                              
REMARK 500  3 ARG B  80         0.27    SIDE_CHAIN                              
REMARK 500  4 ARG A   5         0.26    SIDE_CHAIN                              
REMARK 500  4 ARG A  13         0.25    SIDE_CHAIN                              
REMARK 500  4 ARG A  15         0.28    SIDE_CHAIN                              
REMARK 500  4 ARG A  16         0.27    SIDE_CHAIN                              
REMARK 500  4 ARG A  27         0.31    SIDE_CHAIN                              
REMARK 500  4 ARG A  40         0.32    SIDE_CHAIN                              
REMARK 500  4 ARG A  55         0.28    SIDE_CHAIN                              
REMARK 500  4 ARG A  56         0.30    SIDE_CHAIN                              
REMARK 500  4 ARG A  70         0.31    SIDE_CHAIN                              
REMARK 500  4 ARG A  80         0.30    SIDE_CHAIN                              
REMARK 500  4 ARG B   5         0.26    SIDE_CHAIN                              
REMARK 500  4 ARG B  13         0.25    SIDE_CHAIN                              
REMARK 500  4 ARG B  15         0.28    SIDE_CHAIN                              
REMARK 500  4 ARG B  16         0.27    SIDE_CHAIN                              
REMARK 500  4 ARG B  27         0.31    SIDE_CHAIN                              
REMARK 500  4 ARG B  40         0.32    SIDE_CHAIN                              
REMARK 500  4 ARG B  55         0.28    SIDE_CHAIN                              
REMARK 500  4 ARG B  56         0.30    SIDE_CHAIN                              
REMARK 500  4 ARG B  70         0.30    SIDE_CHAIN                              
REMARK 500  4 ARG B  80         0.30    SIDE_CHAIN                              
REMARK 500  5 ARG A   5         0.23    SIDE_CHAIN                              
REMARK 500  5 ARG A  13         0.29    SIDE_CHAIN                              
REMARK 500  5 ARG A  15         0.19    SIDE_CHAIN                              
REMARK 500  5 ARG A  16         0.17    SIDE_CHAIN                              
REMARK 500  5 ARG A  27         0.32    SIDE_CHAIN                              
REMARK 500  5 ARG A  40         0.17    SIDE_CHAIN                              
REMARK 500  5 ARG A  55         0.31    SIDE_CHAIN                              
REMARK 500  5 ARG A  56         0.21    SIDE_CHAIN                              
REMARK 500  5 ARG A  70         0.32    SIDE_CHAIN                              
REMARK 500  5 ARG A  80         0.32    SIDE_CHAIN                              
REMARK 500  5 ARG B   5         0.23    SIDE_CHAIN                              
REMARK 500  5 ARG B  13         0.29    SIDE_CHAIN                              
REMARK 500  5 ARG B  15         0.19    SIDE_CHAIN                              
REMARK 500  5 ARG B  16         0.17    SIDE_CHAIN                              
REMARK 500  5 ARG B  27         0.32    SIDE_CHAIN                              
REMARK 500  5 ARG B  40         0.17    SIDE_CHAIN                              
REMARK 500  5 ARG B  55         0.31    SIDE_CHAIN                              
REMARK 500  5 ARG B  56         0.21    SIDE_CHAIN                              
REMARK 500  5 ARG B  70         0.32    SIDE_CHAIN                              
REMARK 500  5 ARG B  80         0.32    SIDE_CHAIN                              
REMARK 500  6 ARG A   5         0.10    SIDE_CHAIN                              
REMARK 500  6 ARG A  13         0.28    SIDE_CHAIN                              
REMARK 500  6 ARG A  15         0.08    SIDE_CHAIN                              
REMARK 500  6 ARG A  16         0.31    SIDE_CHAIN                              
REMARK 500  6 ARG A  27         0.30    SIDE_CHAIN                              
REMARK 500  6 ARG A  40         0.32    SIDE_CHAIN                              
REMARK 500  6 ARG A  55         0.30    SIDE_CHAIN                              
REMARK 500  6 ARG A  70         0.32    SIDE_CHAIN                              
REMARK 500  6 ARG A  80         0.27    SIDE_CHAIN                              
REMARK 500  6 ARG B   5         0.10    SIDE_CHAIN                              
REMARK 500  6 ARG B  13         0.28    SIDE_CHAIN                              
REMARK 500  6 ARG B  15         0.08    SIDE_CHAIN                              
REMARK 500  6 ARG B  16         0.31    SIDE_CHAIN                              
REMARK 500  6 ARG B  27         0.30    SIDE_CHAIN                              
REMARK 500  6 ARG B  40         0.32    SIDE_CHAIN                              
REMARK 500  6 ARG B  55         0.30    SIDE_CHAIN                              
REMARK 500  6 ARG B  70         0.32    SIDE_CHAIN                              
REMARK 500  6 ARG B  80         0.27    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: BMRB-8641   RELATED DB: BMRB                             
REMARK 900 THE ASSIGNED CHEMICAL SHIFTS WERE SUBMITTED FOR BMRB                 
REMARK 900 ANALYSIS AND VALIDATION                                              
DBREF  1R05 A    2    83  UNP    P61244   MAX_HUMAN       22    103             
DBREF  1R05 B    2    83  UNP    P61244   MAX_HUMAN       22    103             
SEQADV 1R05 MET A    1  UNP  P61244              INITIATING MET                 
SEQADV 1R05 VAL A   58  UNP  P61244    ASN    78 ENGINEERED                     
SEQADV 1R05 LEU A   61  UNP  P61244    HIS    81 ENGINEERED                     
SEQADV 1R05 GLY A   84  UNP  P61244              CLONING ARTIFACT               
SEQADV 1R05 SER A   85  UNP  P61244              CLONING ARTIFACT               
SEQADV 1R05 GLY A   86  UNP  P61244              CLONING ARTIFACT               
SEQADV 1R05 CYS A   87  UNP  P61244              CLONING ARTIFACT               
SEQADV 1R05 MET B    1  UNP  P61244              INITIATING MET                 
SEQADV 1R05 VAL B   58  UNP  P61244    ASN    78 ENGINEERED                     
SEQADV 1R05 LEU B   61  UNP  P61244    HIS    81 ENGINEERED                     
SEQADV 1R05 GLY B   84  UNP  P61244              CLONING ARTIFACT               
SEQADV 1R05 SER B   85  UNP  P61244              CLONING ARTIFACT               
SEQADV 1R05 GLY B   86  UNP  P61244              CLONING ARTIFACT               
SEQADV 1R05 CYS B   87  UNP  P61244              CLONING ARTIFACT               
SEQRES   1 A   87  MET ALA ASP LYS ARG ALA HIS HIS ASN ALA LEU GLU ARG          
SEQRES   2 A   87  LYS ARG ARG ASP HIS ILE LYS ASP SER PHE HIS SER LEU          
SEQRES   3 A   87  ARG ASP SER VAL PRO SER LEU GLN GLY GLU LYS ALA SER          
SEQRES   4 A   87  ARG ALA GLN ILE LEU ASP LYS ALA THR GLU TYR ILE GLN          
SEQRES   5 A   87  TYR MET ARG ARG LYS VAL HIS THR LEU GLN GLN ASP ILE          
SEQRES   6 A   87  ASP ASP LEU LYS ARG GLN ASN ALA LEU LEU GLU GLN GLN          
SEQRES   7 A   87  VAL ARG ALA LEU GLU GLY SER GLY CYS                          
SEQRES   1 B   87  MET ALA ASP LYS ARG ALA HIS HIS ASN ALA LEU GLU ARG          
SEQRES   2 B   87  LYS ARG ARG ASP HIS ILE LYS ASP SER PHE HIS SER LEU          
SEQRES   3 B   87  ARG ASP SER VAL PRO SER LEU GLN GLY GLU LYS ALA SER          
SEQRES   4 B   87  ARG ALA GLN ILE LEU ASP LYS ALA THR GLU TYR ILE GLN          
SEQRES   5 B   87  TYR MET ARG ARG LYS VAL HIS THR LEU GLN GLN ASP ILE          
SEQRES   6 B   87  ASP ASP LEU LYS ARG GLN ASN ALA LEU LEU GLU GLN GLN          
SEQRES   7 B   87  VAL ARG ALA LEU GLU GLY SER GLY CYS                          
HELIX    1   1 ASP A   17  VAL A   30  1                                  14    
HELIX    2   2 SER A   32  LYS A   37  5                                   6    
HELIX    3   3 ALA A   41  GLU A   83  1                                  43    
HELIX    4   4 ASP B   17  VAL B   30  1                                  14    
HELIX    5   5 SER B   32  LYS B   37  5                                   6    
HELIX    6   6 ALA B   41  GLU B   83  1                                  43    
SSBOND   1 CYS A   87    CYS B   87                          1555   1555  2.02  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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