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Database: PDB
Entry: 1SUL
LinkDB: 1SUL
Original site: 1SUL 
HEADER    HYDROLASE                               26-MAR-04   1SUL              
TITLE     CRYSTAL STRUCTURE OF THE APO-YSXC                                     
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: GTP-BINDING PROTEIN YSXC;                                  
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: BACILLUS SUBTILIS;                              
SOURCE   3 ORGANISM_TAXID: 1423;                                                
SOURCE   4 GENE: YSXC, ENGB, BSU28190;                                          
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    GTP, GTPASE, GTP-BINDING, HYDROLASE                                   
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    S.N.RUZHEINIKOV,K.S.DAS,S.E.SEDELNIKOVA,P.J.BAKER,P.J.ARTYMIUK,       
AUTHOR   2 J.GARCIA-LARA,S.J.FOSTER,D.W.RICE                                    
REVDAT   3   11-OCT-17 1SUL    1       REMARK                                   
REVDAT   2   24-FEB-09 1SUL    1       VERSN                                    
REVDAT   1   25-MAY-04 1SUL    0                                                
JRNL        AUTH   S.N.RUZHEINIKOV,S.K.DAS,S.E.SEDELNIKOVA,P.J.BAKER,           
JRNL        AUTH 2 P.J.ARTYMIUK,J.GARCIA-LARA,S.J.FOSTER,D.W.RICE               
JRNL        TITL   ANALYSIS OF THE OPEN AND CLOSED CONFORMATIONS OF THE         
JRNL        TITL 2 GTP-BINDING PROTEIN YSXC FROM BACILLUS SUBTILIS.             
JRNL        REF    J.MOL.BIOL.                   V. 339   265 2004              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   15136032                                                     
JRNL        DOI    10.1016/J.JMB.2004.03.043                                    
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   S.K.DAS,S.E.SEDELNIKOVA,P.J.BAKER,S.N.RUZHEINIKOV,           
REMARK   1  AUTH 2 S.J.FOSTER,D.W.RICE                                          
REMARK   1  TITL   EXPRESSION, PURIFICATION, CRYSTALLIZATION AND PRELIMINARY    
REMARK   1  TITL 2 CRYSTALLOGRAPHIC ANALYSIS OF A PUTATIVE GTP-BINDING PROTEIN, 
REMARK   1  TITL 3 YSXC, FROM BACILLUS SUBTILIS .                               
REMARK   1  REF    TO BE PUBLISHED                                              
REMARK   1  REFN                                                                
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.00 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.0                                           
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.00                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 10.00                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 96.4                           
REMARK   3   NUMBER OF REFLECTIONS             : 23172                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.177                           
REMARK   3   R VALUE            (WORKING SET) : 0.173                           
REMARK   3   FREE R VALUE                     : 0.251                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.100                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1243                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.00                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.05                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 1593                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL                         
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2410                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 86                           
REMARK   3   BIN FREE R VALUE                    : 0.4140                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 3040                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 506                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 17.42                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -0.30000                                             
REMARK   3    B22 (A**2) : 0.07000                                              
REMARK   3    B33 (A**2) : -0.56000                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : -0.74000                                             
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): 0.224         
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.202         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.146         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 5.208         
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.951                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.902                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  3098 ; 0.014 ; 0.022       
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  4176 ; 1.468 ; 1.961       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   378 ; 3.850 ; 3.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):  NULL ;  NULL ;  NULL       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   619 ;16.599 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):  NULL ;  NULL ;  NULL       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   466 ; 0.107 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  2277 ; 0.006 ; 0.020       
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  1529 ; 0.253 ; 0.300       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   418 ; 0.267 ; 0.500       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):    76 ; 0.233 ; 0.300       
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):    56 ; 0.266 ; 0.500       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  1898 ; 0.829 ; 1.500       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  3081 ; 1.522 ; 2.000       
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  1200 ; 2.542 ; 3.000       
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  1095 ; 4.242 ; 4.500       
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 1                                          
REMARK   3                                                                      
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 2                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A     1        A   195                          
REMARK   3    RESIDUE RANGE :   B     1        B   195                          
REMARK   3    ORIGIN FOR THE GROUP (A):  10.3718 -11.5948  69.9788              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0048 T22:   0.0081                                     
REMARK   3      T33:   0.0272 T12:  -0.0051                                     
REMARK   3      T13:   0.0081 T23:  -0.0026                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.2240 L22:   0.1961                                     
REMARK   3      L33:   0.8498 L12:  -0.0680                                     
REMARK   3      L13:   0.3015 L23:  -0.2146                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0085 S12:  -0.0291 S13:   0.0061                       
REMARK   3      S21:   0.0133 S22:   0.0011 S23:  -0.0006                       
REMARK   3      S31:  -0.0083 S32:  -0.0389 S33:  -0.0096                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : BABINET MODEL WITH MASK                              
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.40                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1SUL COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 20-APR-04.                  
REMARK 100 THE DEPOSITION ID IS D_1000022003.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 01-JAN-01                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 8.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : N                                  
REMARK 200  RADIATION SOURCE               : ROTATING ANODE                     
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU                             
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418                             
REMARK 200  MONOCHROMATOR                  : NI FILTER                          
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE                        
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU                             
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MAR                                
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 24474                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.000                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 10.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : NULL                               
REMARK 200  DATA REDUNDANCY                : 2.400                              
REMARK 200  R MERGE                    (I) : 0.09100                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 9.9000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.00                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.05                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 93.4                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.39200                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.200                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MIR                          
REMARK 200 SOFTWARE USED: MLPHARE                                               
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 42.08                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.12                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: SODIUM ACETATE, PEG 4000, TRIS HCL,      
REMARK 280  MNCL2, PH 8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K      
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       72.83500            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       18.26000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       72.83500            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       18.26000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     ARG A    51                                                      
REMARK 465     THR A    52                                                      
REMARK 465     SER A    53                                                      
REMARK 465     SER A    54                                                      
REMARK 465     LYS A    55                                                      
REMARK 465     PRO A    56                                                      
REMARK 465     GLY A    57                                                      
REMARK 465     LYS A    58                                                      
REMARK 465     THR A    59                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    HOH B   411     O    HOH B   423              1.73            
REMARK 500   O    HOH B   328     O    HOH B   331              1.91            
REMARK 500   O    HOH B   412     O    HOH B   423              1.92            
REMARK 500   O    HOH A   239     O    HOH A   378              1.93            
REMARK 500   O    HOH A   304     O    HOH A   322              1.94            
REMARK 500   O    HOH B   297     O    HOH B   411              1.98            
REMARK 500   O    HOH A   375     O    HOH A   418              1.99            
REMARK 500   OE1  GLU A    90     O    HOH A   274              2.02            
REMARK 500   O    HOH A   286     O    HOH A   443              2.06            
REMARK 500   O    HOH A   313     O    HOH A   363              2.07            
REMARK 500   O    HOH B   287     O    HOH B   357              2.07            
REMARK 500   O    HOH B   286     O    HOH B   318              2.09            
REMARK 500   O    HOH A   267     O    HOH A   322              2.09            
REMARK 500   O    HOH B   280     O    HOH B   346              2.10            
REMARK 500   O    HOH A   357     O    HOH A   447              2.11            
REMARK 500   O    HOH B   245     O    HOH B   262              2.12            
REMARK 500   NZ   LYS A    86     O    HOH A   457              2.12            
REMARK 500   O    HOH B   326     O    HOH B   412              2.12            
REMARK 500   NZ   LYS B   191     O    HOH B   339              2.13            
REMARK 500   OE2  GLU A   128     O    HOH A   233              2.15            
REMARK 500   O    HOH B   335     O    HOH B   342              2.17            
REMARK 500   OE2  GLU A    90     O    HOH A   354              2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   O    HOH A   284     O    HOH B   281     3454     2.09            
REMARK 500   O    HOH A   211     O    HOH B   373     3454     2.11            
REMARK 500   O    HOH A   281     O    HOH B   307     4557     2.17            
REMARK 500   O    HOH A   344     O    HOH B   340     2667     2.17            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    ASP A  69   CB  -  CG  -  OD2 ANGL. DEV. =   5.4 DEGREES          
REMARK 500    ASP A 153   CB  -  CG  -  OD2 ANGL. DEV. =   5.4 DEGREES          
REMARK 500    ASP A 166   CB  -  CG  -  OD2 ANGL. DEV. =   5.8 DEGREES          
REMARK 500    LEU B  28   CA  -  CB  -  CG  ANGL. DEV. =  16.5 DEGREES          
REMARK 500    ARG B  46   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.5 DEGREES          
REMARK 500    ASP B 166   CB  -  CG  -  OD2 ANGL. DEV. =   5.6 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN B  48       49.34   -157.25                                   
REMARK 500    ARG B  51       24.68   -140.98                                   
REMARK 500    GLU B  70       -6.07   -140.33                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1SVI   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF THE GTP-BINDING PROTEIN YSXC COMPLEXED WITH GDP 
REMARK 900 RELATED ID: 1SVW   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF YSXC COMPLEXED WITH GMPPNP                      
DBREF  1SUL A    1   195  UNP    P38424   ENGB_BACSU       1    195             
DBREF  1SUL B    1   195  UNP    P38424   ENGB_BACSU       1    195             
SEQRES   1 A  195  MET LYS VAL THR LYS SER GLU ILE VAL ILE SER ALA VAL          
SEQRES   2 A  195  LYS PRO GLU GLN TYR PRO GLU GLY GLY LEU PRO GLU ILE          
SEQRES   3 A  195  ALA LEU ALA GLY ARG SER ASN VAL GLY LYS SER SER PHE          
SEQRES   4 A  195  ILE ASN SER LEU ILE ASN ARG LYS ASN LEU ALA ARG THR          
SEQRES   5 A  195  SER SER LYS PRO GLY LYS THR GLN THR LEU ASN PHE TYR          
SEQRES   6 A  195  ILE ILE ASN ASP GLU LEU HIS PHE VAL ASP VAL PRO GLY          
SEQRES   7 A  195  TYR GLY PHE ALA LYS VAL SER LYS SER GLU ARG GLU ALA          
SEQRES   8 A  195  TRP GLY ARG MET ILE GLU THR TYR ILE THR THR ARG GLU          
SEQRES   9 A  195  GLU LEU LYS ALA VAL VAL GLN ILE VAL ASP LEU ARG HIS          
SEQRES  10 A  195  ALA PRO SER ASN ASP ASP VAL GLN MET TYR GLU PHE LEU          
SEQRES  11 A  195  LYS TYR TYR GLY ILE PRO VAL ILE VAL ILE ALA THR LYS          
SEQRES  12 A  195  ALA ASP LYS ILE PRO LYS GLY LYS TRP ASP LYS HIS ALA          
SEQRES  13 A  195  LYS VAL VAL ARG GLN THR LEU ASN ILE ASP PRO GLU ASP          
SEQRES  14 A  195  GLU LEU ILE LEU PHE SER SER GLU THR LYS LYS GLY LYS          
SEQRES  15 A  195  ASP GLU ALA TRP GLY ALA ILE LYS LYS MET ILE ASN ARG          
SEQRES   1 B  195  MET LYS VAL THR LYS SER GLU ILE VAL ILE SER ALA VAL          
SEQRES   2 B  195  LYS PRO GLU GLN TYR PRO GLU GLY GLY LEU PRO GLU ILE          
SEQRES   3 B  195  ALA LEU ALA GLY ARG SER ASN VAL GLY LYS SER SER PHE          
SEQRES   4 B  195  ILE ASN SER LEU ILE ASN ARG LYS ASN LEU ALA ARG THR          
SEQRES   5 B  195  SER SER LYS PRO GLY LYS THR GLN THR LEU ASN PHE TYR          
SEQRES   6 B  195  ILE ILE ASN ASP GLU LEU HIS PHE VAL ASP VAL PRO GLY          
SEQRES   7 B  195  TYR GLY PHE ALA LYS VAL SER LYS SER GLU ARG GLU ALA          
SEQRES   8 B  195  TRP GLY ARG MET ILE GLU THR TYR ILE THR THR ARG GLU          
SEQRES   9 B  195  GLU LEU LYS ALA VAL VAL GLN ILE VAL ASP LEU ARG HIS          
SEQRES  10 B  195  ALA PRO SER ASN ASP ASP VAL GLN MET TYR GLU PHE LEU          
SEQRES  11 B  195  LYS TYR TYR GLY ILE PRO VAL ILE VAL ILE ALA THR LYS          
SEQRES  12 B  195  ALA ASP LYS ILE PRO LYS GLY LYS TRP ASP LYS HIS ALA          
SEQRES  13 B  195  LYS VAL VAL ARG GLN THR LEU ASN ILE ASP PRO GLU ASP          
SEQRES  14 B  195  GLU LEU ILE LEU PHE SER SER GLU THR LYS LYS GLY LYS          
SEQRES  15 B  195  ASP GLU ALA TRP GLY ALA ILE LYS LYS MET ILE ASN ARG          
FORMUL   3  HOH   *506(H2 O)                                                    
HELIX    1   1 LYS A   14  TYR A   18  5                                   5    
HELIX    2   2 SER A   32  ILE A   44  1                                  13    
HELIX    3   3 SER A   85  ARG A  103  1                                  19    
HELIX    4   4 SER A  120  TYR A  133  1                                  14    
HELIX    5   5 LYS A  143  ILE A  147  5                                   5    
HELIX    6   6 PRO A  148  GLY A  150  5                                   3    
HELIX    7   7 LYS A  151  ASN A  164  1                                  14    
HELIX    8   8 GLY A  181  ASN A  194  1                                  14    
HELIX    9   9 LYS B   14  TYR B   18  5                                   5    
HELIX   10  10 SER B   32  ILE B   44  1                                  13    
HELIX   11  11 SER B   85  ARG B  103  1                                  19    
HELIX   12  12 SER B  120  TYR B  133  1                                  14    
HELIX   13  13 LYS B  143  ILE B  147  5                                   5    
HELIX   14  14 PRO B  148  GLY B  150  5                                   3    
HELIX   15  15 LYS B  151  ASN B  164  1                                  14    
HELIX   16  16 GLY B  181  ASN B  194  1                                  14    
SHEET    1   A 7 SER A   6  ALA A  12  0                                        
SHEET    2   A 7 LEU A  62  ILE A  67 -1  O  PHE A  64   N  VAL A   9           
SHEET    3   A 7 LEU A  71  ASP A  75 -1  O  LEU A  71   N  ILE A  67           
SHEET    4   A 7 GLU A  25  GLY A  30  1  N  LEU A  28   O  VAL A  74           
SHEET    5   A 7 LEU A 106  ASP A 114  1  O  VAL A 110   N  ALA A  27           
SHEET    6   A 7 VAL A 137  THR A 142  1  O  THR A 142   N  VAL A 113           
SHEET    7   A 7 GLU A 170  LEU A 173  1  O  ILE A 172   N  VAL A 139           
SHEET    1   B 7 SER B   6  ALA B  12  0                                        
SHEET    2   B 7 LEU B  62  ILE B  67 -1  O  PHE B  64   N  VAL B   9           
SHEET    3   B 7 LEU B  71  ASP B  75 -1  O  ASP B  75   N  ASN B  63           
SHEET    4   B 7 GLU B  25  GLY B  30  1  N  ILE B  26   O  VAL B  74           
SHEET    5   B 7 LEU B 106  ASP B 114  1  O  VAL B 110   N  ALA B  27           
SHEET    6   B 7 VAL B 137  THR B 142  1  O  THR B 142   N  VAL B 113           
SHEET    7   B 7 GLU B 170  LEU B 173  1  O  GLU B 170   N  VAL B 139           
CRYST1  145.670   36.520   83.253  90.00 122.16  90.00 C 1 2 1       8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.006865  0.000000  0.004316        0.00000                         
SCALE2      0.000000  0.027382  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.014188        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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