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Database: PDB
Entry: 1W2R
LinkDB: 1W2R
Original site: 1W2R 
HEADER    IMMUNE SYSTEM                           08-JUL-04   1W2R              
TITLE     SOLUTION STRUCTURE OF CR2 SCR 1-2 BY X-RAY SCATTERING                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: COMPLEMENT RECEPTOR TYPE 2 PRECURSOR,;                     
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: CR2 SCR 1-2, COMPLEMENT C3D RECEPTOR,                       
COMPND   5  EPSTEIN-BARR VIRUS RECEPTOR, EBV RECEPTOR, CD21 ANTIGEN;            
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: PICHIA PASTORIS;                                  
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 4922                                        
KEYWDS    IMMUNE SYSTEM, ANALYTICAL ULTRACENTRIFUGATION,                        
KEYWDS   2 THROMBOSPONDIN TYPE I REPEATS, CONSTRAINED MODELLING,                
KEYWDS   3 GLYCOPROTEIN                                                         
EXPDTA    SOLUTION SCATTERING                                                   
NUMMDL    6                                                                     
MDLTYP    CA ATOMS ONLY, CHAIN A                                                
AUTHOR    H.E.GILBERT,J.T.EATON,J.P.HANNAN,V.M.HOLERS,S.J.PERKINS               
REVDAT   3   07-APR-10 1W2R    1       VERSN                                    
REVDAT   2   24-FEB-09 1W2R    1       VERSN                                    
REVDAT   1   29-SEP-05 1W2R    0                                                
JRNL        AUTH   H.E.GILBERT,J.T.EATON,J.P.HANNAN,V.M.HOLERS,                 
JRNL        AUTH 2 S.J.PERKINS                                                  
JRNL        TITL   SOLUTION STRUCTURE OF THE COMPLEX BETWEEN CR2 SCR            
JRNL        TITL 2 1-2 AND C3D OF HUMAN COMPLEMENT: AN X-RAY                    
JRNL        TITL 3 SCATTERING AND SEDIMENTATION MODELLING STUDY.                
JRNL        REF    J.MOL.BIOL.                   V. 346   859 2005              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   15713468                                                     
JRNL        DOI    10.1016/J.JMB.2004.12.006                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : INSIGHT II 98                                        
REMARK   3   AUTHORS     : MSI                                                  
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 142                                     
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 0                                       
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1W2R COMPLIES WITH FORMAT V. 3.20, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 09-JUL-04.                  
REMARK 100 THE PDBE ID CODE IS EBI-20083.                                       
REMARK 265                                                                      
REMARK 265 EXPERIMENTAL DETAILS                                                 
REMARK 265                                                                      
REMARK 265 EXPERIMENT TYPE : SMALL ANGLE X-RAY SCATTERING                       
REMARK 265  DATA ACQUISITION                                                    
REMARK 265   RADIATION/NEUTRON SOURCE                 : ESRF BEAMLINE ID02      
REMARK 265   SYNCHROTRON (Y/N)                        : Y                       
REMARK 265   BEAMLINE TYPE                            : NULL                    
REMARK 265   BEAMLINE INSTRUMENT                      : NULL                    
REMARK 265   DETECTOR TYPE                            : FRELON CCD CAMERA       
REMARK 265   DETECTOR MANUFACTURER DETAILS            : NULL                    
REMARK 265   TEMPERATURE (KELVIN)                     : 288                     
REMARK 265   PH                                       : 7.4                     
REMARK 265   NUMBER OF TIME FRAMES USED               : 1                       
REMARK 265   PROTEIN CONCENTRATION RANGE (MG/ML)      : 0.6-10.2                
REMARK 265   SAMPLE BUFFER                            : 10 MM HEPES,            
REMARK 265                                              50 MM NACL              
REMARK 265   DATA REDUCTION SOFTWARE                  : MULTICCD                
REMARK 265   GUINIER MEAN RADIUS OF GYRATION (NM)     : 2.12                    
REMARK 265   SIGMA MEAN RADIUS OF GYRATION            : 0.05                    
REMARK 265   R(XS-1) MEAN CROSS SECTIONAL RADII (NM)  : 1.00                    
REMARK 265   R(XS-1) SIGMA MEAN CROSS SECTIONAL RADII : 0.10                    
REMARK 265   R(XS-2) MEAN CROSS SECTIONAL RADII (NM)  : NULL                    
REMARK 265   R(XS-2) SIGMA MEAN CROSS SECTIONAL RADII : NULL                    
REMARK 265   P(R) PROTEIN LENGTH (NM)                 : 10                      
REMARK 265                                                                      
REMARK 265 DATA ANALYSIS AND MODEL FITTING:                                     
REMARK 265  METHOD USED TO DETERMINE THE STRUCTURE: NULL                        
REMARK 265  SOFTWARE USED    : INSIGHT II, SCTPL7, GNOM                         
REMARK 265  SOFTWARE AUTHORS : MSI                                              
REMARK 265  STARTING MODEL   : NULL                                             
REMARK 265                                                                      
REMARK 265 CONFORMERS, NUMBER CALCULATED   : NULL                               
REMARK 265 CONFORMERS, NUMBER SUBMITTED    : 6                                  
REMARK 265 CONFORMERS, SELECTION CRITERIA  : NULL                               
REMARK 265                                                                      
REMARK 265 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 265                                                                      
REMARK 265  OTHER DETAILS: NULL                                                 
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY.  THE REMARK MAY ALSO PROVIDE INFORMATION ON              
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE:  1                                                      
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC                  
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1GHQ   RELATED DB: PDB                                   
REMARK 900  CR2-C3D COMPLEX STRUCTURE                                           
REMARK 900 RELATED ID: 1LY2   RELATED DB: PDB                                   
REMARK 900  CRYSTAL STRUCTURE OF UNLIGANDED HUMAN CD21                          
REMARK 900  SCR1-SCR2(COMPLEMENT RECEPTOR TYPE 2)                               
REMARK 900 RELATED ID: 1W2S   RELATED DB: PDB                                   
REMARK 900  SOLUTION STRUCTURE OF CR2 SCR 1-2 IN ITS                            
REMARK 900   COMPLEX WITH C3D BY X-RAY SCATTERING                               
DBREF  1W2R A    1     4  PDB    1W2R     1W2R             1      4             
DBREF  1W2R A    5   137  UNP    P20023   CR2_HUMAN       21    153             
DBREF  1W2R A  138   142  PDB    1W2R     1W2R           138    142             
SEQADV 1W2R GLN A   67  UNP  P20023    GLU    83 CONFLICT                       
SEQRES   1 A  142  GLU ALA GLU ALA ILE SER CYS GLY SER PRO PRO PRO ILE          
SEQRES   2 A  142  LEU ASN GLY ARG ILE SER TYR TYR SER THR PRO ILE ALA          
SEQRES   3 A  142  VAL GLY THR VAL ILE ARG TYR SER CYS SER GLY THR PHE          
SEQRES   4 A  142  ARG LEU ILE GLY GLU LYS SER LEU LEU CYS ILE THR LYS          
SEQRES   5 A  142  ASP LYS VAL ASP GLY THR TRP ASP LYS PRO ALA PRO LYS          
SEQRES   6 A  142  CYS GLN TYR PHE ASN LYS TYR SER SER CYS PRO GLU PRO          
SEQRES   7 A  142  ILE VAL PRO GLY GLY TYR LYS ILE ARG GLY SER THR PRO          
SEQRES   8 A  142  TYR ARG HIS GLY ASP SER VAL THR PHE ALA CYS LYS THR          
SEQRES   9 A  142  ASN PHE SER MET ASN GLY ASN LYS SER VAL TRP CYS GLN          
SEQRES  10 A  142  ALA ASN ASN MET TRP GLY PRO THR ARG LEU PRO THR CYS          
SEQRES  11 A  142  VAL SER VAL PHE PRO LEU GLU GLN LYS LEU ILE SER              
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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