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Database: PDB
Entry: 1Z5S
LinkDB: 1Z5S
Original site: 1Z5S 
HEADER    LIGASE                                  19-MAR-05   1Z5S              
TITLE     CRYSTAL STRUCTURE OF A COMPLEX BETWEEN UBC9, SUMO-1,                  
TITLE    2 RANGAP1 AND NUP358/RANBP2                                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: UBIQUITIN-CONJUGATING ENZYME E2 I;                         
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: UBIQUITIN-PROTEIN LIGASE I, UBIQUITIN CARRIER               
COMPND   5 PROTEIN I, SUMO-1-PROTEIN LIGASE, SUMO- 1 CONJUGATING                
COMPND   6 ENZYME, UBIQUITIN CARRIER PROTEIN 9, P18;                            
COMPND   7 EC: 6.3.2.19;                                                        
COMPND   8 ENGINEERED: YES;                                                     
COMPND   9 MOL_ID: 2;                                                           
COMPND  10 MOLECULE: UBIQUITIN-LIKE PROTEIN SMT3C;                              
COMPND  11 CHAIN: B;                                                            
COMPND  12 SYNONYM: UBIQUITIN-HOMOLOGY DOMAIN PROTEIN PIC1, UBIQUITIN-          
COMPND  13 LIKE PROTEIN UBL1, UBIQUITIN-RELATED PROTEIN SUMO-1, GAP             
COMPND  14 MODIFYING PROTEIN 1, GMP1, SENTRIN, OK/SW-CL.43;                     
COMPND  15 ENGINEERED: YES;                                                     
COMPND  16 MOL_ID: 3;                                                           
COMPND  17 MOLECULE: RAN GTPASE-ACTIVATING PROTEIN 1;                           
COMPND  18 CHAIN: C;                                                            
COMPND  19 FRAGMENT: C-TERMINAL DOMAIN;                                         
COMPND  20 ENGINEERED: YES;                                                     
COMPND  21 MOL_ID: 4;                                                           
COMPND  22 MOLECULE: RAN-BINDING PROTEIN 2;                                     
COMPND  23 CHAIN: D;                                                            
COMPND  24 FRAGMENT: IR1-M DOMAIN;                                              
COMPND  25 SYNONYM: RANBP2, NUCLEAR PORE COMPLEX PROTEIN NUP358,                
COMPND  26 NUCLEOPORIN NUP358, 358 KDA NUCLEOPORIN, P270;                       
COMPND  27 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: UBE2I, UBC9, UBCE9;                                            
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21DE3;                                   
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET28B;                                   
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  13 ORGANISM_COMMON: HUMAN;                                              
SOURCE  14 ORGANISM_TAXID: 9606;                                                
SOURCE  15 GENE: UBL1, SMT3C, SMT3H3;                                           
SOURCE  16 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  17 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  18 EXPRESSION_SYSTEM_STRAIN: BL21DE3;                                   
SOURCE  19 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  20 EXPRESSION_SYSTEM_PLASMID: PET28B;                                   
SOURCE  21 MOL_ID: 3;                                                           
SOURCE  22 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  23 ORGANISM_COMMON: HUMAN;                                              
SOURCE  24 ORGANISM_TAXID: 9606;                                                
SOURCE  25 GENE: RANGAP1;                                                       
SOURCE  26 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  27 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  28 EXPRESSION_SYSTEM_STRAIN: BL21DE3;                                   
SOURCE  29 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  30 EXPRESSION_SYSTEM_PLASMID: PSMT3;                                    
SOURCE  31 MOL_ID: 4;                                                           
SOURCE  32 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  33 ORGANISM_COMMON: HUMAN;                                              
SOURCE  34 ORGANISM_TAXID: 9606;                                                
SOURCE  35 GENE: RANBP2, NUP358;                                                
SOURCE  36 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  37 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  38 EXPRESSION_SYSTEM_STRAIN: BL21DE3;                                   
SOURCE  39 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  40 EXPRESSION_SYSTEM_PLASMID: PSMT3                                     
KEYWDS    E3, LIGASE, SUMO, UBC9, NUCLEAR PORE COMPLEX                          
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    D.REVERTER,C.D.LIMA                                                   
REVDAT   2   24-FEB-09 1Z5S    1       VERSN                                    
REVDAT   1   07-JUN-05 1Z5S    0                                                
JRNL        AUTH   D.REVERTER,C.D.LIMA                                          
JRNL        TITL   INSIGHTS INTO E3 LIGASE ACTIVITY REVEALED BY A               
JRNL        TITL 2 SUMO-RANGAP1-UBC9-NUP358 COMPLEX.                            
JRNL        REF    NATURE                        V. 435   687 2005              
JRNL        REFN                   ISSN 0028-0836                               
JRNL        PMID   15931224                                                     
JRNL        DOI    10.1038/NATURE03588                                          
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.01 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.1                                              
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,             
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : ENGH & HUBER                                    
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.01                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 29.69                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : 2281669.870                    
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : 0.0000                         
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 96.5                           
REMARK   3   NUMBER OF REFLECTIONS             : 16461                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.247                           
REMARK   3   FREE R VALUE                     : 0.290                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.100                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 832                             
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : 0.010                           
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 6                            
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 3.01                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 3.19                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 86.20                        
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : 2299                         
REMARK   3   BIN R VALUE           (WORKING SET) : 0.4250                       
REMARK   3   BIN FREE R VALUE                    : 0.4320                       
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : 5.00                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : 122                          
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : 0.039                        
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 3564                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 28                                      
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 74.00                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 90.00                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -15.45000                                            
REMARK   3    B22 (A**2) : -15.45000                                            
REMARK   3    B33 (A**2) : 30.91000                                             
REMARK   3    B12 (A**2) : 17.61000                                             
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : 0.47                            
REMARK   3   ESD FROM SIGMAA              (A) : 0.99                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : 8.00                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : 0.57                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : 1.16                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.006                           
REMARK   3   BOND ANGLES            (DEGREES) : 1.20                            
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : 21.40                           
REMARK   3   IMPROPER ANGLES        (DEGREES) : 0.84                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : RESTRAINED                                
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : 2.270 ; 2.000                
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : 3.980 ; 3.500                
REMARK   3   SIDE-CHAIN BOND              (A**2) : 2.800 ; 2.500                
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : 4.720 ; 4.000                
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : FLAT MODEL                                           
REMARK   3   KSOL        : 0.27                                                 
REMARK   3   BSOL        : 13.94                                                
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM                              
REMARK   3  PARAMETER FILE  2  : DNA-RNA_REP.PARAM                              
REMARK   3  PARAMETER FILE  3  : WATER_REP.PARAM                                
REMARK   3  PARAMETER FILE  4  : ION.PARAM                                      
REMARK   3  PARAMETER FILE  5  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : PROTEIN.TOP                                    
REMARK   3  TOPOLOGY FILE  2   : DNA-RNA.TOP                                    
REMARK   3  TOPOLOGY FILE  3   : WATER.TOP                                      
REMARK   3  TOPOLOGY FILE  4   : ION.TOP                                        
REMARK   3  TOPOLOGY FILE  5   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: BULK SOLVENT MODEL USED                   
REMARK   4                                                                      
REMARK   4 1Z5S COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 30-MAR-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB032333.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 24-AUG-04                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 31-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9793                             
REMARK 200  MONOCHROMATOR                  : DIAMOND                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARRESEARCH                        
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 16464                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.000                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.0                               
REMARK 200  DATA REDUNDANCY                : NULL                               
REMARK 200  R MERGE                    (I) : 0.07500                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 15.1000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.00                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.11                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 91.8                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.46400                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.100                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: MOLREP                                                
REMARK 200 STARTING MODEL: RANGAP1-UBC9 COMPLEX                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 67.73                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.81                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 18% PEG4000 (W/V), 0.1 M SODIUM          
REMARK 280  CITRATE, 0.2 M AMMONIUM ACETATE, PH 5.0, VAPOR DIFFUSION,           
REMARK 280  HANGING DROP, TEMPERATURE 291K                                      
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 32 2 1                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+2/3                                            
REMARK 290       3555   -X+Y,-X,Z+1/3                                           
REMARK 290       4555   Y,X,-Z                                                  
REMARK 290       5555   X-Y,-Y,-Z+1/3                                           
REMARK 290       6555   -X,-X+Y,-Z+2/3                                          
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       39.74200            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       19.87100            
REMARK 290   SMTRY1   4 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   5  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       19.87100            
REMARK 290   SMTRY1   6 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       39.74200            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     SER A   158                                                      
REMARK 465     MET B    16                                                      
REMARK 465     GLY B    17                                                      
REMARK 465     GLU B    18                                                      
REMARK 465     GLY B    19                                                      
REMARK 465     SER C   416                                                      
REMARK 465     LEU C   417                                                      
REMARK 465     ASN C   418                                                      
REMARK 465     THR C   419                                                      
REMARK 465     GLY C   420                                                      
REMARK 465     GLU C   421                                                      
REMARK 465     PRO C   422                                                      
REMARK 465     ALA C   423                                                      
REMARK 465     PRO C   424                                                      
REMARK 465     VAL C   425                                                      
REMARK 465     LEU C   426                                                      
REMARK 465     SER C   427                                                      
REMARK 465     SER C   428                                                      
REMARK 465     PRO C   429                                                      
REMARK 465     PRO C   430                                                      
REMARK 465     PRO C   431                                                      
REMARK 465     GLU D  2694                                                      
REMARK 465     LYS D  2695                                                      
REMARK 465     CYS D  2696                                                      
REMARK 465     ARG D  2697                                                      
REMARK 465     PRO D  2698                                                      
REMARK 465     LEU D  2699                                                      
REMARK 465     GLU D  2700                                                      
REMARK 465     GLU D  2701                                                      
REMARK 465     ASN D  2702                                                      
REMARK 465     THR D  2703                                                      
REMARK 465     ALA D  2704                                                      
REMARK 465     ASP D  2705                                                      
REMARK 465     ASN D  2706                                                      
REMARK 465     GLU D  2707                                                      
REMARK 465     LYS D  2708                                                      
REMARK 465     GLU D  2709                                                      
REMARK 465     CYS D  2710                                                      
REMARK 465     ILE D  2711                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS(M=MODEL NUMBER;            
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     SER A   2    CB   OG                                             
REMARK 470     LYS A  18    CG   CD   CE   NZ                                   
REMARK 470     LYS A  49    CG   CD   CE   NZ                                   
REMARK 470     GLU B  83    CG   CD   OE1  OE2                                  
REMARK 470     LYS C 452    CG   CD   CE   NZ                                   
REMARK 470     LYS C 553    CG   CD   CE   NZ                                   
REMARK 470     LYS C 586    CG   CD   CE   NZ                                   
REMARK 470     LYS D2650    CD   CE   NZ                                        
REMARK 470     ARG D2663    CD   NE   CZ   NH1  NH2                             
REMARK 470     ASP D2665    OD1  OD2                                            
REMARK 470     TYR D2666    CD1  CD2  CE1  CE2  CZ   OH                         
REMARK 470     GLU D2669    CD   OE1  OE2                                       
REMARK 470     GLU D2670    CD   OE1  OE2                                       
REMARK 470     LYS D2683    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    PRO A  28     -174.63    -63.89                                   
REMARK 500    THR A  29     -164.26   -111.71                                   
REMARK 500    ASP A  33       -7.93    -48.76                                   
REMARK 500    THR A  35     -171.71    -56.25                                   
REMARK 500    TYR A  68      143.62    -29.38                                   
REMARK 500    PRO A  84      -34.88    -35.87                                   
REMARK 500    TYR A  87      162.92    -49.41                                   
REMARK 500    LYS A 101      -90.01   -136.46                                   
REMARK 500    GLU A 122       67.87   -119.45                                   
REMARK 500    ASN A 124       63.57   -107.83                                   
REMARK 500    ILE A 125      -17.78    -49.80                                   
REMARK 500    ASP A 127       79.63    178.40                                   
REMARK 500    ASP B  30       27.84   -166.34                                   
REMARK 500    MET B  40       23.01    -68.20                                   
REMARK 500    GLN B  55       -5.54   -144.84                                   
REMARK 500    MET B  59      -34.00    -39.55                                   
REMARK 500    SER B  61      -41.91    -20.73                                   
REMARK 500    ARG B  63      133.53   -171.33                                   
REMARK 500    THR B  76      145.81   -178.64                                   
REMARK 500    PRO B  77      -12.96    -46.04                                   
REMARK 500    GLU B  83     -152.79    -73.50                                   
REMARK 500    GLU B  85       -0.73     67.23                                   
REMARK 500    ASP B  86      174.38    -49.61                                   
REMARK 500    PRO C 441      174.39    -46.34                                   
REMARK 500    SER C 442      136.08    178.79                                   
REMARK 500    ARG C 448       32.65    -68.54                                   
REMARK 500    LYS C 452       30.87    -91.86                                   
REMARK 500    SER C 454        2.98    -55.66                                   
REMARK 500    VAL C 455      -20.09   -148.58                                   
REMARK 500    ALA C 458       44.85    -69.09                                   
REMARK 500    GLN C 459      -47.72   -166.21                                   
REMARK 500    ASP C 462       82.70    -68.40                                   
REMARK 500    THR C 463        2.96    -63.74                                   
REMARK 500    SER C 464      -81.21    -61.18                                   
REMARK 500    SER C 478        6.59    -56.14                                   
REMARK 500    ASP C 482       -2.93    -52.41                                   
REMARK 500    GLU C 483      172.26    -47.27                                   
REMARK 500    LYS C 500      -71.26    -66.23                                   
REMARK 500    SER C 504       83.80    167.31                                   
REMARK 500    SER C 505      -25.27    -29.95                                   
REMARK 500    SER C 506       44.34    -72.22                                   
REMARK 500    LEU C 513      -35.53    -39.16                                   
REMARK 500    MET C 520       32.67    -90.33                                   
REMARK 500    ASP C 527     -158.91   -141.67                                   
REMARK 500    ALA C 533      -81.57    -53.56                                   
REMARK 500    TYR C 550        4.95    -58.49                                   
REMARK 500    LYS C 553        8.55    -58.04                                   
REMARK 500    SER C 568      -72.75    -30.12                                   
REMARK 500    LEU C 570       12.09    -65.44                                   
REMARK 500    CYS C 573       46.80   -153.06                                   
REMARK 500    PHE C 575      -72.87    -57.97                                   
REMARK 500    PRO D2655      -35.18    -32.18                                   
REMARK 500    THR D2656       52.22    -94.57                                   
REMARK 500    ASP D2665     -145.49    -60.17                                   
REMARK 500    TYR D2666       99.24    -46.22                                   
REMARK 500    SER D2668     -124.74   -163.17                                   
REMARK 500    GLU D2669       49.75   -171.41                                   
REMARK 500    GLU D2670      125.83   -172.04                                   
REMARK 500    GLU D2671      137.23    176.29                                   
REMARK 500    ASP D2674      -31.22   -161.38                                   
REMARK 500    GLU D2675      139.14    -32.83                                   
REMARK 500    ASN D2685      -75.16     18.02                                   
REMARK 500    LYS D2687      173.59    -47.89                                   
REMARK 500    ASP D2691      108.30    -44.98                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1KPS   RELATED DB: PDB                                   
REMARK 900 PUTATIVE SUBSTRATE COMPLEX BETWEEN UBC9 AND RANGAP1                  
REMARK 999                                                                      
REMARK 999 SEQUENCE                                                             
REMARK 999 COVALENT ISOPEPTIDE BOND BETWEEN RANGAP1 LYS524 AND                  
REMARK 999 SUMO C-TERMINUS GLY97.                                               
DBREF  1Z5S A    1   158  UNP    P63279   UBE2I_HUMAN      1    158             
DBREF  1Z5S B   18    97  UNP    P63165   SUMO1_HUMAN     18     97             
DBREF  1Z5S C  418   587  UNP    P46060   RGP1_HUMAN     418    587             
DBREF  1Z5S D 2631  2711  UNP    P49792   RBP2_HUMAN    2631   2711             
SEQADV 1Z5S MET B   16  UNP  P63165              CLONING ARTIFACT               
SEQADV 1Z5S GLY B   17  UNP  P63165              CLONING ARTIFACT               
SEQADV 1Z5S SER C  416  UNP  P46060              CLONING ARTIFACT               
SEQADV 1Z5S LEU C  417  UNP  P46060              CLONING ARTIFACT               
SEQADV 1Z5S SER D 2629  UNP  P49792              CLONING ARTIFACT               
SEQADV 1Z5S LEU D 2630  UNP  P49792              CLONING ARTIFACT               
SEQRES   1 A  158  MET SER GLY ILE ALA LEU SER ARG LEU ALA GLN GLU ARG          
SEQRES   2 A  158  LYS ALA TRP ARG LYS ASP HIS PRO PHE GLY PHE VAL ALA          
SEQRES   3 A  158  VAL PRO THR LYS ASN PRO ASP GLY THR MET ASN LEU MET          
SEQRES   4 A  158  ASN TRP GLU CYS ALA ILE PRO GLY LYS LYS GLY THR PRO          
SEQRES   5 A  158  TRP GLU GLY GLY LEU PHE LYS LEU ARG MET LEU PHE LYS          
SEQRES   6 A  158  ASP ASP TYR PRO SER SER PRO PRO LYS CYS LYS PHE GLU          
SEQRES   7 A  158  PRO PRO LEU PHE HIS PRO ASN VAL TYR PRO SER GLY THR          
SEQRES   8 A  158  VAL CYS LEU SER ILE LEU GLU GLU ASP LYS ASP TRP ARG          
SEQRES   9 A  158  PRO ALA ILE THR ILE LYS GLN ILE LEU LEU GLY ILE GLN          
SEQRES  10 A  158  GLU LEU LEU ASN GLU PRO ASN ILE GLN ASP PRO ALA GLN          
SEQRES  11 A  158  ALA GLU ALA TYR THR ILE TYR CYS GLN ASN ARG VAL GLU          
SEQRES  12 A  158  TYR GLU LYS ARG VAL ARG ALA GLN ALA LYS LYS PHE ALA          
SEQRES  13 A  158  PRO SER                                                      
SEQRES   1 B   82  MET GLY GLU GLY GLU TYR ILE LYS LEU LYS VAL ILE GLY          
SEQRES   2 B   82  GLN ASP SER SER GLU ILE HIS PHE LYS VAL LYS MET THR          
SEQRES   3 B   82  THR HIS LEU LYS LYS LEU LYS GLU SER TYR CYS GLN ARG          
SEQRES   4 B   82  GLN GLY VAL PRO MET ASN SER LEU ARG PHE LEU PHE GLU          
SEQRES   5 B   82  GLY GLN ARG ILE ALA ASP ASN HIS THR PRO LYS GLU LEU          
SEQRES   6 B   82  GLY MET GLU GLU GLU ASP VAL ILE GLU VAL TYR GLN GLU          
SEQRES   7 B   82  GLN THR GLY GLY                                              
SEQRES   1 C  172  SER LEU ASN THR GLY GLU PRO ALA PRO VAL LEU SER SER          
SEQRES   2 C  172  PRO PRO PRO ALA ASP VAL SER THR PHE LEU ALA PHE PRO          
SEQRES   3 C  172  SER PRO GLU LYS LEU LEU ARG LEU GLY PRO LYS SER SER          
SEQRES   4 C  172  VAL LEU ILE ALA GLN GLN THR ASP THR SER ASP PRO GLU          
SEQRES   5 C  172  LYS VAL VAL SER ALA PHE LEU LYS VAL SER SER VAL PHE          
SEQRES   6 C  172  LYS ASP GLU ALA THR VAL ARG MET ALA VAL GLN ASP ALA          
SEQRES   7 C  172  VAL ASP ALA LEU MET GLN LYS ALA PHE ASN SER SER SER          
SEQRES   8 C  172  PHE ASN SER ASN THR PHE LEU THR ARG LEU LEU VAL HIS          
SEQRES   9 C  172  MET GLY LEU LEU LYS SER GLU ASP LYS VAL LYS ALA ILE          
SEQRES  10 C  172  ALA ASN LEU TYR GLY PRO LEU MET ALA LEU ASN HIS MET          
SEQRES  11 C  172  VAL GLN GLN ASP TYR PHE PRO LYS ALA LEU ALA PRO LEU          
SEQRES  12 C  172  LEU LEU ALA PHE VAL THR LYS PRO ASN SER ALA LEU GLU          
SEQRES  13 C  172  SER CYS SER PHE ALA ARG HIS SER LEU LEU GLN THR LEU          
SEQRES  14 C  172  TYR LYS VAL                                                  
SEQRES   1 D   83  SER LEU ASP VAL LEU ILE VAL TYR GLU LEU THR PRO THR          
SEQRES   2 D   83  ALA GLU GLN LYS ALA LEU ALA THR LYS LEU LYS LEU PRO          
SEQRES   3 D   83  PRO THR PHE PHE CYS TYR LYS ASN ARG PRO ASP TYR VAL          
SEQRES   4 D   83  SER GLU GLU GLU GLU ASP ASP GLU ASP PHE GLU THR ALA          
SEQRES   5 D   83  VAL LYS LYS LEU ASN GLY LYS LEU TYR LEU ASP GLY SER          
SEQRES   6 D   83  GLU LYS CYS ARG PRO LEU GLU GLU ASN THR ALA ASP ASN          
SEQRES   7 D   83  GLU LYS GLU CYS ILE                                          
FORMUL   5  HOH   *28(H2 O)                                                     
HELIX    1   1 ILE A    4  ASP A   19  1                                  16    
HELIX    2   2 LEU A   94  GLU A   98  5                                   5    
HELIX    3   3 THR A  108  GLU A  122  1                                  15    
HELIX    4   4 GLN A  130  ASN A  140  1                                  11    
HELIX    5   5 ASN A  140  LYS A  154  1                                  15    
HELIX    6   6 HIS B   43  ARG B   54  1                                  12    
HELIX    7   7 THR B   76  GLY B   81  1                                   6    
HELIX    8   8 ALA C  432  PHE C  440  1                                   9    
HELIX    9   9 LEU C  446  SER C  454  5                                   9    
HELIX   10  10 LEU C  456  THR C  461  1                                   6    
HELIX   11  11 ASP C  465  SER C  478  1                                  14    
HELIX   12  12 ALA C  484  PHE C  502  1                                  19    
HELIX   13  13 ASN C  508  MET C  520  1                                  13    
HELIX   14  14 LEU C  535  VAL C  546  1                                  12    
HELIX   15  15 LEU C  555  LYS C  565  1                                  11    
HELIX   16  16 SER C  568  SER C  572  5                                   5    
HELIX   17  17 CYS C  573  VAL C  587  1                                  15    
HELIX   18  18 THR D 2641  LEU D 2651  1                                  11    
HELIX   19  19 ASP D 2676  LEU D 2684  1                                   9    
SHEET    1   A 4 VAL A  25  LYS A  30  0                                        
SHEET    2   A 4 MET A  36  PRO A  46 -1  O  ASN A  37   N  THR A  29           
SHEET    3   A 4 LEU A  57  LEU A  63 -1  O  PHE A  58   N  ILE A  45           
SHEET    4   A 4 LYS A  74  PHE A  77 -1  O  LYS A  76   N  ARG A  61           
SHEET    1   B 6 GLN B  69  ARG B  70  0                                        
SHEET    2   B 6 PHE B  64  PHE B  66 -1  N  PHE B  66   O  GLN B  69           
SHEET    3   B 6 VAL B  87  VAL B  90 -1  O  GLU B  89   N  LEU B  65           
SHEET    4   B 6 ILE B  22  ILE B  27  1  N  ILE B  27   O  ILE B  88           
SHEET    5   B 6 GLU B  33  VAL B  38 -1  O  PHE B  36   N  LEU B  24           
SHEET    6   B 6 VAL D2632  GLU D2637 -1  O  TYR D2636   N  HIS B  35           
LINK         C   GLY B  97                 NZ  LYS C 524     1555   1555  1.33  
CISPEP   1 TYR A   68    PRO A   69          0        -0.05                     
CISPEP   2 GLU A   78    PRO A   79          0        -0.09                     
CRYST1  157.123  157.123   59.613  90.00  90.00 120.00 P 32 2 1      6          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.006364  0.003675  0.000000        0.00000                         
SCALE2      0.000000  0.007349  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.016775        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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