GenomeNet

Database: PDB
Entry: 2G3Q
LinkDB: 2G3Q
Original site: 2G3Q 
HEADER    ENDOCYTOSIS/SIGNALING PROTEIN           20-FEB-06   2G3Q              
TITLE     SOLUTION STRUCTURE OF EDE1 UBA-UBIQUITIN COMPLEX                      
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN YBL047C;                                           
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: EDE1 UBA, RESIDUES 1339-1381;                              
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: UBIQUITIN;                                                 
COMPND   8 CHAIN: B;                                                            
COMPND   9 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE   3 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE   4 ORGANISM_TAXID: 4932;                                                
SOURCE   5 GENE: EDE1;                                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21;                            
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET30;                                    
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  13 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  14 ORGANISM_TAXID: 4932;                                                
SOURCE  15 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21;                            
SOURCE  16 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE  17 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE  18 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  19 EXPRESSION_SYSTEM_PLASMID: PET30                                     
KEYWDS    ENDOCYTOSIS, MONOUBIQUITIN SIGNALING, SOLUTION STRUCTURE,             
KEYWDS   2 UBA DOMAIN, UBIQUITIN-BINDING MOTIF, ENDOCYTOSIS/SIGNALING           
KEYWDS   3 PROTEIN COMPLEX                                                      
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    K.A.SWANSON,L.HICKE,I.RADHAKRISHNAN                                   
REVDAT   2   24-FEB-09 2G3Q    1       VERSN                                    
REVDAT   1   09-MAY-06 2G3Q    0                                                
JRNL        AUTH   K.A.SWANSON,L.HICKE,I.RADHAKRISHNAN                          
JRNL        TITL   STRUCTURAL BASIS FOR MONOUBIQUITIN RECOGNITION BY            
JRNL        TITL 2 THE EDE1 UBA DOMAIN.                                         
JRNL        REF    J.MOL.BIOL.                   V. 358   713 2006              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   16563434                                                     
JRNL        DOI    10.1016/J.JMB.2006.02.059                                    
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.1, ARIA 1.2                                    
REMARK   3   AUTHORS     : BRUNGER ET AL. (CNS), LINGE AND NILGES (ARIA)        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: THE STRUCTURES ARE BASED ON A TOTAL       
REMARK   3  OF 2489 DISTANCE RESTRAINTS, INCLUDING 2489 NOE-DERIVED             
REMARK   3  DISTANCE RESTRAINTS [2069 UNAMBIGUOUS AND 420 AMBIGUOUS             
REMARK   3  RESTRAINTS], 74 HYDROGEN BONDING DISTANCE RESTRAINTS, AND 161       
REMARK   3  TORSION ANGLE RESTRAINTS.                                           
REMARK   4                                                                      
REMARK   4 2G3Q COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 03-MAR-06.                  
REMARK 100 THE RCSB ID CODE IS RCSB036649.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 6.0                                
REMARK 210  IONIC STRENGTH                 : 20MM SODIUM PHOSPHATE              
REMARK 210  PRESSURE                       : 1 ATM                              
REMARK 210  SAMPLE CONTENTS                : 1MM 15N,13C-LABELED EDE1 UBA;      
REMARK 210                                   1MM UBIQUITIN; 20MM PHOSPHATE      
REMARK 210                                   BUFFER (PH 6.0), 2MM DTT, 0.2%     
REMARK 210                                   NAN3, 90% H2O, 10% D2O; 1MM        
REMARK 210                                   15N,13C-LABELED EDE1 UBA; 1MM      
REMARK 210                                   UBIQUITIN; 20MM PHOSPHATE          
REMARK 210                                   BUFFER (PH 6.0), 2MM DTT, 0.2%     
REMARK 210                                   NAN3, 100% D2O; 1MM EDE1 UBA;      
REMARK 210                                   1MM 15N,13C-LABELED UBIQUITIN;     
REMARK 210                                   20MM PHOSPHATE BUFFER (PH 6.0)     
REMARK 210                                   , 2MM DTT, 0.2% NAN3, 90% H2O,     
REMARK 210                                   10% D2O; 1MM EDE1 UBA; 1MM         
REMARK 210                                   15N,13C-LABELED UBIQUITIN;         
REMARK 210                                   20MM PHOSPHATE BUFFER (PH 6.0)     
REMARK 210                                   , 2MM DTT, 0.2% NAN3, 100% D2O     
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210  13C-SEPARATED_NOESY, 3D_13C-SEPARATED_12C-EDITED_NOESY              
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ                            
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : FELIX 98.0                         
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS AND         
REMARK 210                                   SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 80                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : THE SUBMITTED CONFORMERS ARE       
REMARK 210                                   THE 20 STRUCTURES WITH THE         
REMARK 210                                   LOWEST RESTRAINT ENERGIES,         
REMARK 210                                   RESTRAINT VIOLATIONS, AND RMS      
REMARK 210                                   DEVIATIONS FROM IDEAL COVALENT     
REMARK 210                                   GEOMETRY                           
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: ADDITIONAL EXPERIMENTS: 2D DOUBLE HALF FILTERED NOESY        
REMARK 210  WAS PERFORMED FOR BOTH D2O AND H2O SAMPLES                          
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASN A1367       52.29     93.07                                   
REMARK 500  1 ASP A1379      -64.18    -93.34                                   
REMARK 500  1 GLU B  34      -61.94   -104.08                                   
REMARK 500  1 ILE B  61      106.31    -56.61                                   
REMARK 500  1 GLN B  62     -167.52    -74.65                                   
REMARK 500  1 LEU B  73      163.19     64.61                                   
REMARK 500  2 CYS A1366       -9.95    -58.77                                   
REMARK 500  2 ASN A1367       53.03     93.16                                   
REMARK 500  2 TRP A1368       81.66     39.35                                   
REMARK 500  2 LEU A1370      -74.77    -62.66                                   
REMARK 500  2 ASP A1379      -68.06    -94.12                                   
REMARK 500  2 SER A1380       83.44     33.53                                   
REMARK 500  2 VAL B  17     -162.24   -108.11                                   
REMARK 500  2 GLU B  34      -65.57   -102.77                                   
REMARK 500  2 GLN B  40      -47.27   -134.33                                   
REMARK 500  2 ASN B  60       89.94     56.40                                   
REMARK 500  2 LEU B  73     -177.32     60.13                                   
REMARK 500  2 ARG B  74       96.97     59.13                                   
REMARK 500  3 ASN A1367       52.83     95.03                                   
REMARK 500  3 TRP A1368       72.21     37.07                                   
REMARK 500  3 LEU A1370      -76.10    -53.62                                   
REMARK 500  3 ASP A1379      -64.98    -92.94                                   
REMARK 500  3 SER A1380      170.11    -55.48                                   
REMARK 500  3 LYS B  33      -66.69   -104.36                                   
REMARK 500  3 GLU B  34      -75.41    -92.39                                   
REMARK 500  3 ALA B  46       19.84     59.01                                   
REMARK 500  3 ASN B  60       93.41     57.58                                   
REMARK 500  3 LEU B  73      109.62     61.80                                   
REMARK 500  4 MET A1352      -65.66   -128.67                                   
REMARK 500  4 CYS A1366       -8.94    -58.07                                   
REMARK 500  4 ASN A1367       55.54     91.64                                   
REMARK 500  4 TRP A1368       82.00     36.68                                   
REMARK 500  4 LEU A1370      -73.41    -57.31                                   
REMARK 500  4 ASP A1379      -62.51    -91.06                                   
REMARK 500  4 SER A1380      170.80    -58.47                                   
REMARK 500  4 LYS B  33      -63.05    -97.76                                   
REMARK 500  4 GLU B  34      -73.93    -94.79                                   
REMARK 500  4 GLN B  40      -47.86   -130.98                                   
REMARK 500  4 ASN B  60       93.02     56.75                                   
REMARK 500  4 LEU B  73      -83.52     63.42                                   
REMARK 500  5 PRO A1341      -66.07    -93.41                                   
REMARK 500  5 ASN A1367       54.67     96.25                                   
REMARK 500  5 TRP A1368       59.34     39.51                                   
REMARK 500  5 ASP A1379      -65.68    -92.05                                   
REMARK 500  5 VAL B  17     -168.65   -128.32                                   
REMARK 500  5 LYS B  33      -68.06    -93.91                                   
REMARK 500  5 GLU B  34      -63.61    -94.41                                   
REMARK 500  5 ALA B  46       18.56     58.70                                   
REMARK 500  5 ASN B  60       15.59     59.88                                   
REMARK 500  5 LEU B  71     -146.61     98.25                                   
REMARK 500  5 ARG B  72       89.57     71.79                                   
REMARK 500  6 ASN A1367       52.45     95.76                                   
REMARK 500  6 TRP A1368       66.96     37.57                                   
REMARK 500  6 LEU A1370      -75.81    -57.49                                   
REMARK 500  6 LYS B  33      -66.58   -105.75                                   
REMARK 500  6 GLU B  34      -71.21    -80.82                                   
REMARK 500  6 ASN B  60       90.31     55.00                                   
REMARK 500  6 LEU B  71     -142.15     96.69                                   
REMARK 500  6 ARG B  74     -172.66     55.55                                   
REMARK 500  7 PRO A1341      -65.51    -91.84                                   
REMARK 500  7 ASN A1367       51.86     95.91                                   
REMARK 500  7 TRP A1368       82.94     42.89                                   
REMARK 500  7 LEU A1370      -78.35    -56.24                                   
REMARK 500  7 SER A1380     -178.30    -68.49                                   
REMARK 500  7 LYS B  33      -67.79   -102.85                                   
REMARK 500  7 ASN B  60       18.56     58.44                                   
REMARK 500  7 LEU B  73     -164.71     46.64                                   
REMARK 500  8 ASN A1367       54.57     94.11                                   
REMARK 500  8 TRP A1368       85.35     36.68                                   
REMARK 500  8 LEU A1370      -76.57    -58.99                                   
REMARK 500  8 LEU A1378      -65.41    -95.14                                   
REMARK 500  8 LYS B  33      -68.36    -90.18                                   
REMARK 500  8 GLU B  34      -71.32    -86.80                                   
REMARK 500  8 ALA B  46       17.10     57.59                                   
REMARK 500  8 LEU B  73      174.55     64.50                                   
REMARK 500  9 ASN A1367       52.82     94.85                                   
REMARK 500  9 ASP A1379      -66.46    -92.14                                   
REMARK 500  9 GLU B  34      -61.63    -91.56                                   
REMARK 500  9 ALA B  46       14.27     57.78                                   
REMARK 500  9 ASN B  60       92.81     59.01                                   
REMARK 500  9 GLN B  62     -174.71    -67.64                                   
REMARK 500  9 GLU B  64       19.88     58.42                                   
REMARK 500  9 LEU B  73      164.48     64.04                                   
REMARK 500 10 MET A1352      -58.78   -121.25                                   
REMARK 500 10 ASN A1367       53.87     94.42                                   
REMARK 500 10 TRP A1368       82.14     39.18                                   
REMARK 500 10 LEU A1370      -73.98    -62.35                                   
REMARK 500 10 ASP A1379      -67.43    -94.10                                   
REMARK 500 10 SER A1380       89.58     39.81                                   
REMARK 500 10 GLU B  34      -68.72   -130.71                                   
REMARK 500 10 ALA B  46       19.63     54.88                                   
REMARK 500 10 ASN B  60       96.58     57.46                                   
REMARK 500 10 SER B  65     -179.32    -63.93                                   
REMARK 500 10 LEU B  73      160.29     64.84                                   
REMARK 500 11 CYS A1366       -9.57    -58.94                                   
REMARK 500 11 ASN A1367       53.78     92.39                                   
REMARK 500 11 TRP A1368       74.31     39.49                                   
REMARK 500 11 LEU A1370      -77.93    -49.76                                   
REMARK 500 11 SER A1380     -179.93    -60.64                                   
REMARK 500 11 VAL B  17     -169.88   -112.36                                   
REMARK 500 11 GLU B  34      -69.41   -131.60                                   
REMARK 500 11 GLU B  64       19.90     57.06                                   
REMARK 500 11 LEU B  71     -131.40     92.69                                   
REMARK 500 12 PRO A1341      -76.87    -72.21                                   
REMARK 500 12 MET A1352      -51.32   -133.11                                   
REMARK 500 12 ASN A1367       52.11     96.38                                   
REMARK 500 12 TRP A1368       73.80     38.93                                   
REMARK 500 12 LEU A1370      -77.64    -59.11                                   
REMARK 500 12 LYS B  33      -66.30    -94.73                                   
REMARK 500 12 GLU B  34      -74.95    -91.07                                   
REMARK 500 12 GLN B  62     -167.79    -79.97                                   
REMARK 500 12 LEU B  73      121.90     66.10                                   
REMARK 500 13 ASN A1367       53.26     93.25                                   
REMARK 500 13 TRP A1368       81.49     41.31                                   
REMARK 500 13 LEU A1370      -78.52    -55.58                                   
REMARK 500 13 ASP A1379      -68.13    -94.26                                   
REMARK 500 13 SER A1380       33.67     38.75                                   
REMARK 500 13 LYS B  33      -60.96    -90.46                                   
REMARK 500 13 GLU B  34      -75.57    -89.88                                   
REMARK 500 13 ALA B  46       18.92     57.52                                   
REMARK 500 13 ASN B  60       89.66     56.17                                   
REMARK 500 13 SER B  65      170.07    -58.75                                   
REMARK 500 13 LEU B  73      170.45     65.01                                   
REMARK 500 14 ASN A1367       52.92     93.20                                   
REMARK 500 14 TRP A1368       77.79     37.58                                   
REMARK 500 14 LEU A1370      -78.10    -60.46                                   
REMARK 500 14 ASP A1379      -68.28    -94.78                                   
REMARK 500 14 SER A1380     -158.74     41.37                                   
REMARK 500 14 LYS B  33      -68.59   -106.45                                   
REMARK 500 14 ASN B  60       96.85     59.66                                   
REMARK 500 14 LEU B  71     -140.63     88.52                                   
REMARK 500 14 ARG B  72       54.72     73.07                                   
REMARK 500 15 ASN A1367       49.57     97.27                                   
REMARK 500 15 TRP A1368       78.92     41.62                                   
REMARK 500 15 LEU A1370      -73.76    -60.53                                   
REMARK 500 15 SER A1380      174.91    -57.47                                   
REMARK 500 15 VAL B  17     -169.60   -117.39                                   
REMARK 500 15 ASN B  60       91.56     59.01                                   
REMARK 500 15 ARG B  72      -71.90    -50.78                                   
REMARK 500 16 THR A1340       99.12     38.57                                   
REMARK 500 16 MET A1352      -68.33   -122.46                                   
REMARK 500 16 ASN A1367       53.63     92.53                                   
REMARK 500 16 TRP A1368       77.44     39.65                                   
REMARK 500 16 ASP A1379      -63.82    -93.13                                   
REMARK 500 16 GLU B  34      -76.46   -116.28                                   
REMARK 500 16 ALA B  46       19.44     59.39                                   
REMARK 500 16 LEU B  50       99.51    -64.86                                   
REMARK 500 16 GLU B  64       19.90     58.06                                   
REMARK 500 16 LEU B  73      112.19     66.23                                   
REMARK 500 17 ASN A1367       51.45     94.48                                   
REMARK 500 17 TRP A1368       80.40     40.05                                   
REMARK 500 17 LEU A1370      -75.76    -62.90                                   
REMARK 500 17 GLU B  34      -65.33   -103.65                                   
REMARK 500 17 GLN B  40      -54.58   -121.15                                   
REMARK 500 17 ILE B  61      106.89    -59.18                                   
REMARK 500 17 GLN B  62     -173.31    -69.84                                   
REMARK 500 17 GLU B  64       29.24     43.78                                   
REMARK 500 17 ARG B  72      -76.57    -56.30                                   
REMARK 500 17 LEU B  73       32.23   -177.39                                   
REMARK 500 17 ARG B  74       92.23     56.96                                   
REMARK 500 18 ASN A1367       49.81     93.83                                   
REMARK 500 18 TRP A1368       72.94     39.28                                   
REMARK 500 18 LEU A1370      -77.52    -65.45                                   
REMARK 500 18 SER A1380      171.15    -57.23                                   
REMARK 500 18 GLU B  34      -71.13    -85.58                                   
REMARK 500 18 ASN B  60       91.20     57.95                                   
REMARK 500 18 GLU B  64       19.69     58.65                                   
REMARK 500 18 LEU B  73     -173.98     58.82                                   
REMARK 500 18 ARG B  74      141.95     65.66                                   
REMARK 500 19 ASN A1367       53.98     90.77                                   
REMARK 500 19 LEU A1370      -71.58    -62.71                                   
REMARK 500 19 GLU B  34      -74.97    -93.39                                   
REMARK 500 19 ASN B  60       19.98     57.61                                   
REMARK 500 19 ILE B  61      104.68    -59.69                                   
REMARK 500 19 GLU B  64       17.66     58.82                                   
REMARK 500 19 LEU B  71     -134.04    166.54                                   
REMARK 500 19 ARG B  74      -87.76     55.74                                   
REMARK 500 20 MET A1352      -75.83    -92.95                                   
REMARK 500 20 THR A1355      178.53    -59.42                                   
REMARK 500 20 ASN A1367       52.64     95.05                                   
REMARK 500 20 TRP A1368       80.87     36.24                                   
REMARK 500 20 LEU A1370      -78.19    -52.01                                   
REMARK 500 20 ASP A1379      -66.11    -93.07                                   
REMARK 500 20 SER A1380      171.15    -55.21                                   
REMARK 500 20 LYS B  33      -68.18    -90.11                                   
REMARK 500 20 GLU B  34      -70.72   -102.39                                   
REMARK 500 20 GLU B  64       17.47     59.83                                   
REMARK 500 20 LEU B  73      123.91     67.93                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 650                                                                      
REMARK 650 HELIX                                                                
REMARK 650 DETERMINATION METHOD: AUTHOR                                         
DBREF  2G3Q A 1339  1381  UNP    P34216   YBE7_YEAST    1339   1381             
DBREF  2G3Q B    1    76  UNP    P61864   UBIQ_YEAST       1     76             
SEQRES   1 A   43  THR THR PRO LYS SER LEU ALA VAL GLU GLU LEU SER GLY          
SEQRES   2 A   43  MET GLY PHE THR GLU GLU GLU ALA HIS ASN ALA LEU GLU          
SEQRES   3 A   43  LYS CYS ASN TRP ASP LEU GLU ALA ALA THR ASN PHE LEU          
SEQRES   4 A   43  LEU ASP SER ALA                                              
SEQRES   1 B   76  MET GLN ILE PHE VAL LYS THR LEU THR GLY LYS THR ILE          
SEQRES   2 B   76  THR LEU GLU VAL GLU SER SER ASP THR ILE ASP ASN VAL          
SEQRES   3 B   76  LYS SER LYS ILE GLN ASP LYS GLU GLY ILE PRO PRO ASP          
SEQRES   4 B   76  GLN GLN ARG LEU ILE PHE ALA GLY LYS GLN LEU GLU ASP          
SEQRES   5 B   76  GLY ARG THR LEU SER ASP TYR ASN ILE GLN LYS GLU SER          
SEQRES   6 B   76  THR LEU HIS LEU VAL LEU ARG LEU ARG GLY GLY                  
HELIX    1   1 PRO A 1341  SER A 1350  1                                  10    
HELIX    2   2 GLU A 1356  CYS A 1366  1                                  11    
HELIX    3   3 LEU A 1370  LEU A 1378  1                                   9    
HELIX    4   4 ILE B   23  LYS B   33  1                                  11    
HELIX    5   5 SER B   57  TYR B   59  5                                   3    
SHEET    1   A 5 LYS B  11  GLU B  16  0                                        
SHEET    2   A 5 GLN B   2  THR B   7 -1  N  VAL B   5   O  ILE B  13           
SHEET    3   A 5 THR B  66  VAL B  70  1  O  LEU B  69   N  LYS B   6           
SHEET    4   A 5 ARG B  42  PHE B  45 -1  N  ILE B  44   O  HIS B  68           
SHEET    5   A 5 LYS B  48  GLN B  49 -1  O  LYS B  48   N  PHE B  45           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system