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Database: PDB
Entry: 2HYM
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HEADER    IMMUNE SYSTEM                           07-AUG-06   2HYM              
TITLE     NMR BASED DOCKING MODEL OF THE COMPLEX BETWEEN THE HUMAN              
TITLE    2 TYPE I INTERFERON RECEPTOR AND HUMAN INTERFERON ALPHA-2              
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: SOLUBLE IFN ALPHA/BETA RECEPTOR;                           
COMPND   3 CHAIN: A;                                                            
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: INTERFERON ALPHA-2;                                        
COMPND   7 CHAIN: B;                                                            
COMPND   8 SYNONYM: INTERFERON ALPHA-A, LEIF A, INTERFERON ALPHA 2A;            
COMPND   9 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: IFNABR;                                                        
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  10 ORGANISM_COMMON: HUMAN;                                              
SOURCE  11 ORGANISM_TAXID: 9606;                                                
SOURCE  12 GENE: IFNA2;                                                         
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    INTERFERON RECEPTOR COMPLEX, IMMUNE SYSTEM                            
EXPDTA    SOLUTION NMR                                                          
NUMMDL    10                                                                    
AUTHOR    S.R.QUADT-AKABAYOV,J.H.CHILL,R.LEVY,N.KESSLER,J.ANGLISTER             
REVDAT   3   24-FEB-09 2HYM    1       VERSN                                    
REVDAT   2   05-DEC-06 2HYM    1       JRNL                                     
REVDAT   1   10-OCT-06 2HYM    0                                                
JRNL        AUTH   S.R.QUADT-AKABAYOV,J.H.CHILL,R.LEVY,N.KESSLER,               
JRNL        AUTH 2 J.ANGLISTER                                                  
JRNL        TITL   DETERMINATION OF THE HUMAN TYPE I INTERFERON                 
JRNL        TITL 2 RECEPTOR BINDING SITE ON HUMAN INTERFERON-ALPHA2             
JRNL        TITL 3 BY CROSS SATURATION AND AN NMR-BASED MODEL OF THE            
JRNL        TITL 4 COMPLEX                                                      
JRNL        REF    PROTEIN SCI.                  V.  15  2656 2006              
JRNL        REFN                   ISSN 0961-8368                               
JRNL        PMID   17001036                                                     
JRNL        DOI    10.1110/PS.062283006                                         
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : HADDOCK 1.3                                          
REMARK   3   AUTHORS     : DOMINGUEZ, BOELENS, BONVIN                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: 1000 CONFORMERS WERE OBTAINED IN THE      
REMARK   3  RIGID BODY DOCKING AND 200 BEST CONFORMERS WERE SELECTED FOR        
REMARK   3  SEMI-FLEXIBLE SIMULATED ANNEALING FOLLOWED BY REFINEMENT            
REMARK   4                                                                      
REMARK   4 2HYM COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 08-AUG-06.                  
REMARK 100 THE RCSB ID CODE IS RCSB038915.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 308                                
REMARK 210  PH                             : 8                                  
REMARK 210  IONIC STRENGTH                 : 20 MM DEUTERATED TRIS BUFFER       
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.3 MM INTERFERON-ALPHA2 (D,       
REMARK 210                                   15N), 90% D2O, 10% H2O, 20 MM      
REMARK 210                                   DEUTERATED TRIS BUFFER, 0.02%      
REMARK 210                                   NAN3; 0.3 MM INTERFERON-ALPHA2     
REMARK 210                                   (D,15N, 13C), 5% D2O, 95% H2O,     
REMARK 210                                   20 MM DEUTERATED TRIS BUFFER,      
REMARK 210                                   0.02% NAN3; 0.3 MM INTERFERON-     
REMARK 210                                   ALPHA2 (D,15N), 5% D2O, 95%        
REMARK 210                                   H2O, 20 MM DEUTERATED TRIS         
REMARK 210                                   BUFFER, 0.02% NAN3                 
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : TROSY HSQC WITH CROSS              
REMARK 210                                   SATURATION, 3D_15N-SEPARATED_      
REMARK 210                                   NOESY, HNCO, HNCA, HNCACB,         
REMARK 210                                   HNCOCA, HNCACO, HNCB, HNCOCACB     
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 800 MHZ                   
REMARK 210  SPECTROMETER MODEL             : DMX, DRX                           
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 3, NMRPIPE 2.3,            
REMARK 210                                   NMRVIEW 5.2.2                      
REMARK 210   METHOD USED                   : RIGID BODY DOCKING, SEMI-          
REMARK 210                                   FLEXIBLE SIMULATED ANNEALING,      
REMARK 210                                   REFINEMENT USING EXPLICIT WATER    
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 200                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : LOWEST ENERGY STRUCTURES OF        
REMARK 210                                   LOWEST ENERGY CLUSTER              
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 6                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASP A   3     -127.58     39.15                                   
REMARK 500  1 THR A   8       38.70    -81.69                                   
REMARK 500  1 GLU A  10       82.58   -162.36                                   
REMARK 500  1 PHE A  21       12.30     55.24                                   
REMARK 500  1 HIS A  32      -71.86    -89.13                                   
REMARK 500  1 THR A  44     -168.70   -172.08                                   
REMARK 500  1 ASP A  51       49.35    -83.33                                   
REMARK 500  1 ASN A  57      -25.30     77.87                                   
REMARK 500  1 SER A  94       47.58   -154.04                                   
REMARK 500  1 ASP A 104      -90.48    -94.90                                   
REMARK 500  1 SER A 163       31.16    -94.01                                   
REMARK 500  1 ASP A 171     -168.25   -111.33                                   
REMARK 500  1 ASN A 176       60.48     66.12                                   
REMARK 500  1 HIS A 187      -81.13   -134.58                                   
REMARK 500  1 LYS A 195       99.86    -63.08                                   
REMARK 500  1 PRO A 197      106.71    -48.77                                   
REMARK 500  1 PRO A 204      117.22    -36.68                                   
REMARK 500  1 GLN A 207       44.47    -95.17                                   
REMARK 500  1 LYS B  31      -53.49   -162.66                                   
REMARK 500  1 PHE B  36      -70.66   -113.68                                   
REMARK 500  1 PRO B  39       81.33    -68.50                                   
REMARK 500  1 PHE B  43      -51.75   -135.81                                   
REMARK 500  1 GLN B  46      -46.64     72.97                                   
REMARK 500  1 GLN B  48       20.75   -152.26                                   
REMARK 500  1 LYS B  49       83.25   -159.77                                   
REMARK 500  1 ALA B  50      -29.91     70.17                                   
REMARK 500  1 THR B 106       76.67   -103.51                                   
REMARK 500  1 PRO B 109     -158.88    -68.46                                   
REMARK 500  1 SER B 154      -86.59   -122.25                                   
REMARK 500  1 ARG B 162      -82.61    -82.39                                   
REMARK 500  2 ASP A   3     -146.05     41.74                                   
REMARK 500  2 ASN A  20       64.56     60.29                                   
REMARK 500  2 PHE A  21       19.41     53.59                                   
REMARK 500  2 HIS A  32      -78.78   -130.66                                   
REMARK 500  2 LYS A  48       84.58   -153.77                                   
REMARK 500  2 ASP A 104      -83.82    -78.61                                   
REMARK 500  2 SER A 106       91.50    -65.20                                   
REMARK 500  2 ASN A 119       17.88   -141.13                                   
REMARK 500  2 LYS A 159       95.94    -57.50                                   
REMARK 500  2 ASP A 171     -158.23   -111.70                                   
REMARK 500  2 ASN A 176       54.78     70.28                                   
REMARK 500  2 HIS A 187      -79.23   -147.15                                   
REMARK 500  2 ASP A 189     -167.76   -165.06                                   
REMARK 500  2 ALA A 192       37.26    -98.55                                   
REMARK 500  2 PRO A 204      111.14    -30.67                                   
REMARK 500  2 GLU A 208     -166.01    -78.50                                   
REMARK 500  2 HIS B   7      119.82   -169.03                                   
REMARK 500  2 ILE B  24     -158.41   -112.72                                   
REMARK 500  2 ASP B  32       51.87    -99.58                                   
REMARK 500  2 PRO B  39       88.84    -68.24                                   
REMARK 500  2 PHE B  43      -51.96   -133.02                                   
REMARK 500  2 GLN B  46      -36.17     73.68                                   
REMARK 500  2 PRO B 109     -153.07    -72.72                                   
REMARK 500  2 ARG B 162      -91.76    -84.36                                   
REMARK 500  3 ASP A   3     -161.74     44.68                                   
REMARK 500  3 TYR A   7       59.15    -90.92                                   
REMARK 500  3 ASP A   9      -25.89   -152.20                                   
REMARK 500  3 ASN A  20       62.99     61.88                                   
REMARK 500  3 PHE A  21       14.06     57.09                                   
REMARK 500  3 HIS A  32      -80.43   -119.31                                   
REMARK 500  3 THR A  44     -174.31   -177.89                                   
REMARK 500  3 ASP A  51       43.93    -85.89                                   
REMARK 500  3 ASN A  57      -31.46     74.45                                   
REMARK 500  3 ASN A  89       17.16   -153.71                                   
REMARK 500  3 ASP A 104      -95.90   -132.89                                   
REMARK 500  3 SER A 106       98.21    -61.38                                   
REMARK 500  3 GLU A 108      -48.29   -173.61                                   
REMARK 500  3 ILE A 158       37.93    -92.88                                   
REMARK 500  3 SER A 163       42.31    -85.03                                   
REMARK 500  3 ASN A 176       61.19     69.55                                   
REMARK 500  3 HIS A 187     -111.87   -130.69                                   
REMARK 500  3 ALA A 192       38.69    -84.87                                   
REMARK 500  3 PRO A 197      107.12    -53.97                                   
REMARK 500  3 PRO A 204      110.04    -32.97                                   
REMARK 500  3 GLN A 207       33.57    -81.64                                   
REMARK 500  3 PHE A 211     -156.01   -122.40                                   
REMARK 500  3 ARG B  22      109.35    -58.32                                   
REMARK 500  3 LYS B  23      -73.00    -73.67                                   
REMARK 500  3 LEU B  30       47.54    -88.36                                   
REMARK 500  3 LYS B  31      -62.81   -158.73                                   
REMARK 500  3 HIS B  34       98.19   -173.09                                   
REMARK 500  3 PRO B  39       79.56    -69.38                                   
REMARK 500  3 PHE B  43      -38.47   -135.89                                   
REMARK 500  3 GLN B  46      -40.75     71.00                                   
REMARK 500  3 GLN B  48      -71.80   -131.05                                   
REMARK 500  3 ALA B  50      -27.54     69.99                                   
REMARK 500  3 VAL B 105      -60.28   -123.74                                   
REMARK 500  3 PRO B 109     -157.13    -70.37                                   
REMARK 500  3 SER B 154      -84.76   -122.55                                   
REMARK 500  3 ARG B 162      -78.16   -130.96                                   
REMARK 500  4 ASP A   3     -160.48     46.76                                   
REMARK 500  4 THR A   8       30.78    -80.03                                   
REMARK 500  4 ASN A  20       66.46     60.24                                   
REMARK 500  4 PHE A  21       19.46     52.53                                   
REMARK 500  4 HIS A  32      -44.39   -130.06                                   
REMARK 500  4 THR A  44     -166.05   -177.97                                   
REMARK 500  4 ASN A  57      -10.70     77.86                                   
REMARK 500  4 LEU A  68       58.70   -145.70                                   
REMARK 500  4 ASN A  89       19.15   -151.49                                   
REMARK 500  4 ASP A 104      -91.09   -119.99                                   
REMARK 500  4 SER A 106       96.40    -65.83                                   
REMARK 500  4 PHE A 107       50.58   -100.59                                   
REMARK 500  4 ASN A 119       10.70   -141.42                                   
REMARK 500  4 ASP A 171     -161.57   -114.40                                   
REMARK 500  4 ASN A 176       63.16     64.31                                   
REMARK 500  4 SER A 188       54.67   -163.35                                   
REMARK 500  4 PRO A 204      114.84    -33.94                                   
REMARK 500  4 PHE A 211     -159.22   -100.24                                   
REMARK 500  4 ASP B   2       75.48   -115.21                                   
REMARK 500  4 THR B   6      -62.62   -129.89                                   
REMARK 500  4 LYS B  23      -72.71    -87.26                                   
REMARK 500  4 LYS B  31      -57.50   -165.65                                   
REMARK 500  4 ARG B  33       42.91   -157.24                                   
REMARK 500  4 PRO B  39       98.22    -62.56                                   
REMARK 500  4 GLU B  42       44.46    -88.74                                   
REMARK 500  4 PHE B  43      -58.13   -154.30                                   
REMARK 500  4 GLN B  48      -13.55   -143.05                                   
REMARK 500  4 LYS B  49       44.08    -95.07                                   
REMARK 500  4 VAL B 105      -66.51   -126.54                                   
REMARK 500  4 PRO B 109     -158.43    -68.23                                   
REMARK 500  4 LEU B 153      -69.77   -126.58                                   
REMARK 500  4 ARG B 162      -71.60   -163.14                                   
REMARK 500  5 ASP A   3     -153.58     47.95                                   
REMARK 500  5 ASP A   9      -99.00   -149.11                                   
REMARK 500  5 PHE A  21       19.66     50.19                                   
REMARK 500  5 SER A  33      -65.19   -123.89                                   
REMARK 500  5 THR A  44     -168.03   -176.09                                   
REMARK 500  5 ASP A  51       42.65    -85.25                                   
REMARK 500  5 ASN A  57      -34.16     74.38                                   
REMARK 500  5 THR A  69      -62.96   -109.02                                   
REMARK 500  5 TRP A  72       74.67   -117.59                                   
REMARK 500  5 ASN A  89      -39.81   -143.18                                   
REMARK 500  5 SER A  94       52.57   -141.56                                   
REMARK 500  5 ASP A 104      -86.72   -131.99                                   
REMARK 500  5 SER A 106       94.32    -63.72                                   
REMARK 500  5 GLU A 108      -43.40   -138.05                                   
REMARK 500  5 SER A 129       91.57    -69.77                                   
REMARK 500  5 GLN A 136       59.78    -93.36                                   
REMARK 500  5 ASP A 171     -161.64   -117.83                                   
REMARK 500  5 HIS A 187      -73.04   -107.94                                   
REMARK 500  5 SER A 188       36.70   -148.78                                   
REMARK 500  5 PRO A 204      114.59    -35.73                                   
REMARK 500  5 GLN A 207       35.80    -89.94                                   
REMARK 500  5 LYS B  23      -70.96    -88.65                                   
REMARK 500  5 ILE B  24     -119.30   -103.20                                   
REMARK 500  5 HIS B  34       94.29   -175.42                                   
REMARK 500  5 PRO B  39       82.10    -68.80                                   
REMARK 500  5 PHE B  43      -54.55   -131.35                                   
REMARK 500  5 GLN B  48      -48.40   -140.77                                   
REMARK 500  5 ALA B  50      -17.97     68.84                                   
REMARK 500  5 VAL B 105      -78.31   -121.04                                   
REMARK 500  5 PRO B 109     -153.07    -69.71                                   
REMARK 500  5 ARG B 162      -90.07    -82.53                                   
REMARK 500  6 ASP A   3     -155.90     45.06                                   
REMARK 500  6 ASP A   9      -48.37   -137.57                                   
REMARK 500  6 PHE A  21       18.44     52.10                                   
REMARK 500  6 HIS A  32      -74.27   -124.70                                   
REMARK 500  6 ASN A  57      -30.73     77.23                                   
REMARK 500  6 ASN A  89       11.55   -146.43                                   
REMARK 500  6 ASP A 104      -95.33   -141.39                                   
REMARK 500  6 SER A 106      105.08    -57.70                                   
REMARK 500  6 GLU A 108      -46.54   -162.80                                   
REMARK 500  6 LYS A 159      107.09    -56.71                                   
REMARK 500  6 LYS A 172       30.19     72.63                                   
REMARK 500  6 ASN A 176       68.88     68.91                                   
REMARK 500  6 GLU A 186     -172.60   -170.19                                   
REMARK 500  6 HIS A 187      -83.34   -147.21                                   
REMARK 500  6 VAL A 193       89.81    -67.85                                   
REMARK 500  6 PRO A 204      114.50    -36.35                                   
REMARK 500  6 PHE A 211     -157.97   -133.43                                   
REMARK 500  6 LYS B  23      -91.91    -91.94                                   
REMARK 500  6 LYS B  31      -54.21   -171.07                                   
REMARK 500  6 ASP B  32       59.52    -90.53                                   
REMARK 500  6 HIS B  34       83.36   -163.04                                   
REMARK 500  6 PRO B  39       91.86    -64.65                                   
REMARK 500  6 PHE B  43      -53.70   -135.28                                   
REMARK 500  6 GLN B  48      -51.24   -155.35                                   
REMARK 500  6 ALA B  50      -13.95     68.93                                   
REMARK 500  6 VAL B 105      -49.84   -131.08                                   
REMARK 500  6 GLU B 107       13.25   -152.60                                   
REMARK 500  6 PRO B 109     -163.45    -75.55                                   
REMARK 500  6 LEU B 153      -53.22   -121.00                                   
REMARK 500  6 ARG B 162      -85.37    -88.87                                   
REMARK 500  7 ASP A   3     -137.79     40.64                                   
REMARK 500  7 PHE A  21       13.35     55.55                                   
REMARK 500  7 HIS A  32      -80.71   -127.30                                   
REMARK 500  7 THR A  44     -164.90   -172.24                                   
REMARK 500  7 ASN A  57      -34.40     75.64                                   
REMARK 500  7 THR A  69      -40.62   -150.57                                   
REMARK 500  7 ASN A  89       25.29   -146.88                                   
REMARK 500  7 SER A  94       65.91   -151.18                                   
REMARK 500  7 ASP A 104      -87.15    -70.20                                   
REMARK 500  7 SER A 106       95.24    -67.63                                   
REMARK 500  7 GLU A 108      -47.51   -174.09                                   
REMARK 500  7 GLN A 136       42.34    -96.52                                   
REMARK 500  7 ASP A 171     -167.73   -111.64                                   
REMARK 500  7 LYS A 172       34.32     70.30                                   
REMARK 500  7 ASN A 176       63.97     66.63                                   
REMARK 500  7 HIS A 187      -56.88   -154.50                                   
REMARK 500  7 SER A 188       36.81   -157.69                                   
REMARK 500  7 ASP A 189      149.17   -172.92                                   
REMARK 500  7 PRO A 204      109.70    -32.31                                   
REMARK 500  7 GLN A 207       39.82    -88.11                                   
REMARK 500  7 PHE A 211     -169.82   -119.28                                   
REMARK 500  7 ASP B   2       72.16   -118.62                                   
REMARK 500  7 LYS B  23      -82.56    -89.12                                   
REMARK 500  7 ASP B  32       39.89    -92.37                                   
REMARK 500  7 ARG B  33       48.30    -81.03                                   
REMARK 500  7 PHE B  36      -61.11   -101.76                                   
REMARK 500  7 PHE B  43      -46.82   -136.89                                   
REMARK 500  7 GLN B  48      -39.98   -139.93                                   
REMARK 500  7 ALA B  50      -29.97     68.90                                   
REMARK 500  7 VAL B 105      -50.81   -125.31                                   
REMARK 500  7 PRO B 109     -159.67    -63.44                                   
REMARK 500  7 SER B 154      -82.31   -125.99                                   
REMARK 500  7 ARG B 162      -66.69    -90.65                                   
REMARK 500  8 ASP A   3     -171.06     51.21                                   
REMARK 500  8 PHE A  21       17.48     56.33                                   
REMARK 500  8 SER A  33      -61.96   -133.26                                   
REMARK 500  8 ASN A  57      -30.28     72.76                                   
REMARK 500  8 ASP A 104      -92.93   -114.75                                   
REMARK 500  8 GLU A 108      -43.48   -139.54                                   
REMARK 500  8 LYS A 159      106.01    -56.93                                   
REMARK 500  8 ASP A 171     -163.07   -105.44                                   
REMARK 500  8 HIS A 187      -67.59   -144.47                                   
REMARK 500  8 SER A 188       21.78   -152.08                                   
REMARK 500  8 ASP A 189      135.59   -173.79                                   
REMARK 500  8 PRO A 197      107.56    -44.76                                   
REMARK 500  8 PRO A 204      110.40    -37.86                                   
REMARK 500  8 GLN A 207       44.50    -79.26                                   
REMARK 500  8 SER B   8       94.18   -163.48                                   
REMARK 500  8 ARG B  22      109.16    -55.90                                   
REMARK 500  8 HIS B  34      108.82   -170.51                                   
REMARK 500  8 PRO B  39       69.33    -69.64                                   
REMARK 500  8 PHE B  43      -54.58   -138.96                                   
REMARK 500  8 GLN B  46      -58.21     72.89                                   
REMARK 500  8 PRO B 109     -157.27    -70.10                                   
REMARK 500  8 SER B 154      -80.63   -131.11                                   
REMARK 500  8 ARG B 162      -82.98   -125.89                                   
REMARK 500  9 ASP A   3     -146.41     40.75                                   
REMARK 500  9 ASN A  20       74.71     58.18                                   
REMARK 500  9 PHE A  21       18.61     50.82                                   
REMARK 500  9 HIS A  32      -50.62   -125.55                                   
REMARK 500  9 ASN A  57      -35.11     72.57                                   
REMARK 500  9 ASN A  89       12.29   -155.18                                   
REMARK 500  9 ILE A 103      -14.23   -171.45                                   
REMARK 500  9 ASP A 104      -85.68   -105.67                                   
REMARK 500  9 SER A 106       87.89    -67.78                                   
REMARK 500  9 ASP A 171     -165.24   -123.29                                   
REMARK 500  9 LYS A 172       48.37     70.59                                   
REMARK 500  9 ASN A 176       73.98     68.95                                   
REMARK 500  9 HIS A 187      -62.42   -122.85                                   
REMARK 500  9 SER A 188       29.19   -153.66                                   
REMARK 500  9 PRO A 204      110.88    -31.11                                   
REMARK 500  9 GLN A 207       40.82    -99.53                                   
REMARK 500  9 PHE A 211     -137.06   -137.47                                   
REMARK 500  9 LYS B  23      -82.37    -92.07                                   
REMARK 500  9 PHE B  36      -69.42   -103.42                                   
REMARK 500  9 PRO B  39       79.74    -68.14                                   
REMARK 500  9 PHE B  43      -55.96   -138.50                                   
REMARK 500  9 LYS B  49       76.09   -101.38                                   
REMARK 500  9 ALA B  50       -8.47     68.32                                   
REMARK 500  9 ALA B  75      -54.08   -121.97                                   
REMARK 500  9 VAL B 105      -56.63   -131.26                                   
REMARK 500  9 PRO B 109     -156.45    -65.30                                   
REMARK 500  9 SER B 154      -83.75   -126.43                                   
REMARK 500  9 ARG B 162      -71.64   -136.05                                   
REMARK 500 10 ASP A   3     -166.10     47.47                                   
REMARK 500 10 THR A   8       41.28    -85.42                                   
REMARK 500 10 ASN A  20       66.26     62.81                                   
REMARK 500 10 HIS A  32      -63.58   -101.69                                   
REMARK 500 10 THR A  44     -168.18   -173.15                                   
REMARK 500 10 ASP A  51       38.04    -85.51                                   
REMARK 500 10 ASN A  57      -29.44     75.20                                   
REMARK 500 10 THR A  69      -70.38    -77.84                                   
REMARK 500 10 ASP A 104      -90.77   -145.55                                   
REMARK 500 10 SER A 106       92.94    -62.32                                   
REMARK 500 10 GLU A 108      -42.77   -155.47                                   
REMARK 500 10 ASP A 138       88.35   -150.29                                   
REMARK 500 10 LYS A 159      107.43    -58.89                                   
REMARK 500 10 ASP A 171     -153.94   -132.57                                   
REMARK 500 10 HIS A 187      -73.33   -113.77                                   
REMARK 500 10 ALA A 192       40.91    -89.86                                   
REMARK 500 10 PRO A 204      126.16    -39.14                                   
REMARK 500 10 GLN A 207       33.66    -91.97                                   
REMARK 500 10 PRO B  39       75.41    -69.29                                   
REMARK 500 10 PHE B  43      -53.48   -138.42                                   
REMARK 500 10 ALA B  50      -25.22     67.36                                   
REMARK 500 10 THR B 106       71.40   -110.51                                   
REMARK 500 10 PRO B 109     -154.48    -70.12                                   
REMARK 500 10 SER B 154      -79.06   -127.88                                   
REMARK 500 10 ARG B 162      -72.25   -143.87                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1N6V   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF THE UNCOMPLEXED RECEPTOR CHAIN                          
REMARK 900 RELATED ID: 1ITF   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF UNCOMPLEXED INTERFERON ALPHA-2                          
DBREF  2HYM A    1   212  UNP    Q15467   Q15467_HUMAN    28    239             
DBREF  2HYM B    1   165  UNP    P01563   IFNA2_HUMAN     24    188             
SEQRES   1 A  212  SER TYR ASP SER PRO ASP TYR THR ASP GLU SER CYS THR          
SEQRES   2 A  212  PHE LYS ILE SER LEU ARG ASN PHE ARG SER ILE LEU SER          
SEQRES   3 A  212  TRP GLU LEU LYS ASN HIS SER ILE VAL PRO THR HIS TYR          
SEQRES   4 A  212  THR LEU LEU TYR THR ILE MET SER LYS PRO GLU ASP LEU          
SEQRES   5 A  212  LYS VAL VAL LYS ASN CYS ALA ASN THR THR ARG SER PHE          
SEQRES   6 A  212  CYS ASP LEU THR ASP GLU TRP ARG SER THR HIS GLU ALA          
SEQRES   7 A  212  TYR VAL THR VAL LEU GLU GLY PHE SER GLY ASN THR THR          
SEQRES   8 A  212  LEU PHE SER CYS SER HIS ASN PHE TRP LEU ALA ILE ASP          
SEQRES   9 A  212  MET SER PHE GLU PRO PRO GLU PHE GLU ILE VAL GLY PHE          
SEQRES  10 A  212  THR ASN HIS ILE ASN VAL MET VAL LYS PHE PRO SER ILE          
SEQRES  11 A  212  VAL GLU GLU GLU LEU GLN PHE ASP LEU SER LEU VAL ILE          
SEQRES  12 A  212  GLU GLU GLN SER GLU GLY ILE VAL LYS LYS HIS LYS PRO          
SEQRES  13 A  212  GLU ILE LYS GLY ASN MET SER GLY ASN PHE THR TYR ILE          
SEQRES  14 A  212  ILE ASP LYS LEU ILE PRO ASN THR ASN TYR CYS VAL SER          
SEQRES  15 A  212  VAL TYR LEU GLU HIS SER ASP GLU GLN ALA VAL ILE LYS          
SEQRES  16 A  212  SER PRO LEU LYS CYS THR LEU LEU PRO PRO GLY GLN GLU          
SEQRES  17 A  212  SER GLU PHE SER                                              
SEQRES   1 B  165  CYS ASP LEU PRO GLN THR HIS SER LEU GLY SER ARG ARG          
SEQRES   2 B  165  THR LEU MET LEU LEU ALA GLN MET ARG LYS ILE SER LEU          
SEQRES   3 B  165  PHE SER CYS LEU LYS ASP ARG HIS ASP PHE GLY PHE PRO          
SEQRES   4 B  165  GLN GLU GLU PHE GLY ASN GLN PHE GLN LYS ALA GLU THR          
SEQRES   5 B  165  ILE PRO VAL LEU HIS GLU MET ILE GLN GLN ILE PHE ASN          
SEQRES   6 B  165  LEU PHE SER THR LYS ASP SER SER ALA ALA TRP ASP GLU          
SEQRES   7 B  165  THR LEU LEU ASP LYS PHE TYR THR GLU LEU TYR GLN GLN          
SEQRES   8 B  165  LEU ASN ASP LEU GLU ALA CYS VAL ILE GLN GLY VAL GLY          
SEQRES   9 B  165  VAL THR GLU THR PRO LEU MET LYS GLU ASP SER ILE LEU          
SEQRES  10 B  165  ALA VAL ARG LYS TYR PHE GLN ARG ILE THR LEU TYR LEU          
SEQRES  11 B  165  LYS GLU LYS LYS TYR SER PRO CYS ALA TRP GLU VAL VAL          
SEQRES  12 B  165  ARG ALA GLU ILE MET ARG SER PHE SER LEU SER THR ASN          
SEQRES  13 B  165  LEU GLN GLU SER LEU ARG SER LYS GLU                          
HELIX    1   1 VAL A  131  LEU A  135  5                                   5    
HELIX    2   2 LEU B    9  MET B   21  1                                  13    
HELIX    3   3 SER B   25  CYS B   29  5                                   5    
HELIX    4   4 PRO B   39  PHE B   43  5                                   5    
HELIX    5   5 THR B   52  SER B   68  1                                  17    
HELIX    6   6 THR B   69  TRP B   76  1                                   8    
HELIX    7   7 ASP B   77  GLN B  101  1                                  25    
HELIX    8   8 LEU B  110  LYS B  133  1                                  24    
HELIX    9   9 SER B  136  LEU B  153  1                                  18    
HELIX   10  10 SER B  154  GLU B  159  5                                   6    
SHEET    1   A 4 PHE A  65  ASP A  67  0                                        
SHEET    2   A 4 SER A  23  GLU A  28 -1  N  LEU A  25   O  CYS A  66           
SHEET    3   A 4 THR A  13  LEU A  18 -1  N  LYS A  15   O  SER A  26           
SHEET    4   A 4 SER A 106  PHE A 107  1  O  SER A 106   N  LEU A  18           
SHEET    1   B 4 LYS A  53  VAL A  54  0                                        
SHEET    2   B 4 HIS A  38  ILE A  45 -1  N  TYR A  43   O  LYS A  53           
SHEET    3   B 4 TYR A  79  SER A  87 -1  O  VAL A  82   N  LEU A  42           
SHEET    4   B 4 THR A  90  PHE A  93 -1  O  LEU A  92   N  GLY A  85           
SHEET    1   C 4 LYS A  53  VAL A  54  0                                        
SHEET    2   C 4 HIS A  38  ILE A  45 -1  N  TYR A  43   O  LYS A  53           
SHEET    3   C 4 TYR A  79  SER A  87 -1  O  VAL A  82   N  LEU A  42           
SHEET    4   C 4 SER A  96  PHE A  99 -1  O  PHE A  99   N  TYR A  79           
SHEET    1   D 3 GLU A 111  GLY A 116  0                                        
SHEET    2   D 3 HIS A 120  LYS A 126 -1  O  MET A 124   N  GLU A 113           
SHEET    3   D 3 ASN A 165  ASP A 171 -1  O  PHE A 166   N  VAL A 125           
SHEET    1   E 4 ILE A 150  HIS A 154  0                                        
SHEET    2   E 4 SER A 140  SER A 147 -1  N  ILE A 143   O  HIS A 154           
SHEET    3   E 4 ASN A 178  GLU A 186 -1  O  TYR A 184   N  VAL A 142           
SHEET    4   E 4 LYS A 199  LEU A 202 -1  O  THR A 201   N  TYR A 179           
SSBOND   1 CYS A   12    CYS A   95                          1555   1555  2.03  
SSBOND   2 CYS A   58    CYS A   66                          1555   1555  2.03  
SSBOND   3 CYS A  180    CYS A  200                          1555   1555  2.03  
SSBOND   4 CYS B    1    CYS B   98                          1555   1555  2.03  
SSBOND   5 CYS B   29    CYS B  138                          1555   1555  2.04  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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