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Database: PDB
Entry: 2KB5
LinkDB: 2KB5
Original site: 2KB5 
HEADER    HYDROLASE                               20-NOV-08   2KB5              
TITLE     SOLUTION NMR STRUCTURE OF EOSINOPHIL CATIONIC PROTEIN/RNASE           
TITLE    2 3                                                                    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: EOSINOPHIL CATIONIC PROTEIN;                               
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: ECP, RIBONUCLEASE 3, RNASE 3;                               
COMPND   5 EC: 3.1.27.-;                                                        
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: RNASE3, ECP, RNS3;                                             
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE   9 EXPRESSION_SYSTEM_VARIANT: DE3;                                      
SOURCE  10 EXPRESSION_SYSTEM_VECTOR: PET11C                                     
KEYWDS    RIBONUCLEASE, ANTIBIOTIC, ANTIMICROBIAL, ENDONUCLEASE,                
KEYWDS   2 GLYCOPROTEIN, HYDROLASE, POLYMORPHISM                                
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    M.RICO,M.BRUIX,D.V.LAURENTS,J.SANTORO,M.JIMENEZ,E.BOIX,               
AUTHOR   2 M.MOUSSAOUI,M.NOGUES                                                 
REVDAT   2   06-OCT-09 2KB5    1       JRNL                                     
REVDAT   1   23-JUN-09 2KB5    0                                                
JRNL        AUTH   D.V.LAURENTS,M.BRUIX,M.A.JIMENEZ,J.SANTORO,E.BOIX,           
JRNL        AUTH 2 M.MOUSSAOUI,M.V.NOGUES,M.RICO                                
JRNL        TITL   THE (1)H, (13)C, (15)N RESONANCE ASSIGNMENT,                 
JRNL        TITL 2 SOLUTION STRUCTURE, AND RESIDUE LEVEL STABILITY OF           
JRNL        TITL 3 EOSINOPHIL CATIONIC PROTEIN/RNASE 3 DETERMINED BY            
JRNL        TITL 4 NMR SPECTROSCOPY                                             
JRNL        REF    BIOPOLYMERS                   V.  91  1018 2009              
JRNL        REFN                   ISSN 0006-3525                               
JRNL        PMID   19189375                                                     
JRNL        DOI    10.1002/BIP.21152                                            
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA                                                
REMARK   3   AUTHORS     : GUNTERT, MUMENTHALER AND WUTHRICH                    
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2KB5 COMPLIES WITH FORMAT V. 3.20, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 21-NOV-08.                  
REMARK 100 THE RCSB ID CODE IS RCSB100902.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 4.0                                
REMARK 210  IONIC STRENGTH                 : <0.1                               
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1 MM [U-95% 15N] ECP, 50 MM        
REMARK 210                                   POTASSIUM PHOSPHATE, TRACES        
REMARK 210                                   DSS, <1 MM POTASSIUM CHLORIDE,     
REMARK 210                                   95% H2O/5% D2O; 1 MM [U-95%        
REMARK 210                                   15N] ECP, TRACES DSS, 50 MM        
REMARK 210                                   POTASSIUM PHOSPHATE, <10 MM        
REMARK 210                                   SODIUM CHLORIDE, 100% D2O; 1       
REMARK 210                                   MM [U-95% 13C; U-95% 15N] ECP,     
REMARK 210                                   TRACES DSS, 50 MM POTASSIUM        
REMARK 210                                   PHOSPHATE, <10 MM SODIUM           
REMARK 210                                   CHLORIDE, 90% H2O/10% D2O          
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D 1H-1H COSY; 2D 1H-1H            
REMARK 210                                   NOESY; 2D 1H-1H TOCSY; 2D 1H-      
REMARK 210                                   15N HSQC; 3D 1H-15N TOCSY; 3D      
REMARK 210                                   1H-15N NOESY; 3D HNCA; 3D          
REMARK 210                                   HNCO; 3D HN(CO)CA; 3D HNCACB;      
REMARK 210                                   3D CBCA(CO)NH; 3D HCCH-TOCSY;      
REMARK 210                                   3D HCCC-TOCSY                      
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XEASY                              
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : TARGET FUNCTION                    
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 PRO A   3        0.20    -69.72                                   
REMARK 500  1 THR A   6     -174.32    -69.55                                   
REMARK 500  1 SER A  17      105.04   -170.03                                   
REMARK 500  1 PRO A  21     -162.66    -69.83                                   
REMARK 500  1 CYS A  23      -25.15    -39.25                                   
REMARK 500  1 ARG A  28      -76.10    -90.53                                   
REMARK 500  1 ILE A  30      -74.27    -70.80                                   
REMARK 500  1 GLN A  58      108.03    -46.59                                   
REMARK 500  1 LEU A  85       96.18    -65.50                                   
REMARK 500  1 GLN A  91       61.09   -118.97                                   
REMARK 500  1 PHE A 106     -175.11    -68.98                                   
REMARK 500  1 PRO A 116        3.10    -69.79                                   
REMARK 500  1 THR A 131     -175.77   -171.03                                   
REMARK 500  2 PRO A  21     -161.78    -69.81                                   
REMARK 500  2 CYS A  23      -28.28    -36.76                                   
REMARK 500  2 ARG A  28      -75.80    -90.46                                   
REMARK 500  2 ILE A  30      -70.29    -72.81                                   
REMARK 500  2 GLN A  40      140.19   -175.28                                   
REMARK 500  2 ARG A  45       78.85    -67.72                                   
REMARK 500  2 GLN A  58      107.80    -46.51                                   
REMARK 500  2 THR A  67      -54.66   -132.34                                   
REMARK 500  2 LEU A  85      106.36    -57.66                                   
REMARK 500  2 SER A 119       68.19   -118.23                                   
REMARK 500  2 THR A 131     -178.13   -171.15                                   
REMARK 500  3 ALA A   8      -71.62    -89.79                                   
REMARK 500  3 PRO A  21     -161.69    -69.83                                   
REMARK 500  3 CYS A  23      -30.63    -35.71                                   
REMARK 500  3 ILE A  30      -72.19    -70.27                                   
REMARK 500  3 ASN A  41      113.82   -166.43                                   
REMARK 500  3 GLN A  58      108.07    -46.39                                   
REMARK 500  3 SER A  74      113.17    -38.31                                   
REMARK 500  3 PRO A 116        3.49    -69.72                                   
REMARK 500  4 THR A   6     -174.64    -61.56                                   
REMARK 500  4 PRO A  21     -161.71    -69.84                                   
REMARK 500  4 CYS A  23      -29.00    -35.95                                   
REMARK 500  4 GLN A  58      107.91    -46.45                                   
REMARK 500  4 THR A  67      -49.35   -132.82                                   
REMARK 500  4 ASN A  95       53.55   -118.04                                   
REMARK 500  4 PRO A 116        6.06    -69.86                                   
REMARK 500  5 SER A  17      105.25   -172.43                                   
REMARK 500  5 PRO A  21     -160.27    -69.75                                   
REMARK 500  5 CYS A  23      -27.78    -37.15                                   
REMARK 500  5 MET A  27       30.61    -83.87                                   
REMARK 500  5 ARG A  28      -77.07    -83.47                                   
REMARK 500  5 ILE A  30      -70.31    -63.61                                   
REMARK 500  5 ASN A  31      -18.01    -49.00                                   
REMARK 500  5 GLN A  58      108.27    -46.51                                   
REMARK 500  5 ASN A 113      106.61    -54.35                                   
REMARK 500  5 PRO A 116        2.99    -69.76                                   
REMARK 500  6 THR A   6     -174.34    -64.57                                   
REMARK 500  6 SER A  17      105.03   -171.66                                   
REMARK 500  6 PRO A  21     -162.02    -69.82                                   
REMARK 500  6 CYS A  23      -39.83    -31.73                                   
REMARK 500  6 ILE A  30      -74.06    -72.11                                   
REMARK 500  6 GLN A  58      107.86    -46.39                                   
REMARK 500  6 LEU A  85       99.78    -45.90                                   
REMARK 500  6 GLN A  91       61.03   -118.51                                   
REMARK 500  6 PRO A 116        6.21    -69.76                                   
REMARK 500  6 THR A 131     -176.46   -171.50                                   
REMARK 500  7 SER A  17      105.50   -171.62                                   
REMARK 500  7 PRO A  21     -161.46    -69.70                                   
REMARK 500  7 CYS A  23      -27.88    -37.05                                   
REMARK 500  7 ARG A  28      -76.14    -84.08                                   
REMARK 500  7 ILE A  30      -73.35    -65.69                                   
REMARK 500  7 GLN A  40      136.07   -175.28                                   
REMARK 500  7 GLN A  58      105.31    -46.40                                   
REMARK 500  7 LEU A  85      107.85    -51.72                                   
REMARK 500  7 PRO A 116        3.54    -69.80                                   
REMARK 500  8 THR A   6     -174.28    -65.57                                   
REMARK 500  8 ALA A   8      -71.81    -91.27                                   
REMARK 500  8 SER A  17      105.68   -170.61                                   
REMARK 500  8 PRO A  21     -163.05    -69.84                                   
REMARK 500  8 CYS A  23      -33.59    -32.64                                   
REMARK 500  8 ASN A  32       -9.56    -58.98                                   
REMARK 500  8 GLN A  40      145.69   -175.15                                   
REMARK 500  8 GLN A  58      107.90    -46.57                                   
REMARK 500  8 SER A  74      121.84    -38.45                                   
REMARK 500  8 ASN A  95       41.53   -102.26                                   
REMARK 500  8 ASN A 113      105.03    -54.08                                   
REMARK 500  8 PRO A 116        3.85    -69.79                                   
REMARK 500  8 THR A 131     -177.53   -171.72                                   
REMARK 500  9 PRO A   3        0.51    -69.80                                   
REMARK 500  9 ALA A   8      -72.41    -82.33                                   
REMARK 500  9 SER A  17      105.86   -171.92                                   
REMARK 500  9 PRO A  21     -162.25    -69.77                                   
REMARK 500  9 CYS A  23      -26.91    -38.23                                   
REMARK 500  9 ILE A  30      -73.05    -67.80                                   
REMARK 500  9 GLN A  58      107.75    -47.06                                   
REMARK 500  9 GLN A  91       61.04   -118.62                                   
REMARK 500  9 PRO A 116        3.30    -69.74                                   
REMARK 500  9 THR A 131     -178.95   -172.30                                   
REMARK 500 10 ALA A   8      -67.31    -90.07                                   
REMARK 500 10 SER A  17      104.95   -166.81                                   
REMARK 500 10 PRO A  21     -161.00    -69.78                                   
REMARK 500 10 CYS A  23      -41.53    -28.62                                   
REMARK 500 10 ILE A  30      -70.94    -69.64                                   
REMARK 500 10 GLN A  58      108.70    -46.26                                   
REMARK 500 10 THR A  67      -32.82   -134.60                                   
REMARK 500 10 LEU A  85       94.79    -58.96                                   
REMARK 500 10 ASN A  92      106.16   -160.14                                   
REMARK 500 10 ASN A 113      106.18    -54.31                                   
REMARK 500 10 PRO A 116        3.03    -69.73                                   
REMARK 500 11 THR A   6     -174.46    -69.84                                   
REMARK 500 11 PRO A  21     -160.28    -69.79                                   
REMARK 500 11 ARG A  28      -74.99    -87.13                                   
REMARK 500 11 ARG A  45       77.74    -69.71                                   
REMARK 500 11 GLN A  58      105.40    -46.76                                   
REMARK 500 11 LEU A  85       89.93    -47.83                                   
REMARK 500 11 GLN A  91       61.03   -118.49                                   
REMARK 500 11 PRO A 116        3.22    -69.66                                   
REMARK 500 11 THR A 131     -175.00   -171.91                                   
REMARK 500 12 SER A  17      114.95   -169.30                                   
REMARK 500 12 PRO A  21     -161.83    -69.74                                   
REMARK 500 12 CYS A  23      -32.78    -33.76                                   
REMARK 500 12 ASN A  41      135.77   -174.97                                   
REMARK 500 12 ARG A  45       79.61    -61.02                                   
REMARK 500 12 GLN A  58      107.79    -46.48                                   
REMARK 500 12 PRO A 116        4.08    -69.70                                   
REMARK 500 12 THR A 131     -178.35   -172.35                                   
REMARK 500 13 PRO A   3        0.38    -69.81                                   
REMARK 500 13 THR A   6     -174.50    -67.08                                   
REMARK 500 13 ALA A   8      -72.14    -93.12                                   
REMARK 500 13 SER A  17      104.98   -164.74                                   
REMARK 500 13 PRO A  21     -161.59    -69.79                                   
REMARK 500 13 CYS A  23      -41.47    -27.85                                   
REMARK 500 13 ALA A  26      -37.90    -39.85                                   
REMARK 500 13 MET A  27       30.09    -87.47                                   
REMARK 500 13 ILE A  30      -71.82    -74.36                                   
REMARK 500 13 ASN A  32      -18.25    -49.40                                   
REMARK 500 13 GLN A  91       61.03   -118.66                                   
REMARK 500 13 PRO A 116        5.19    -69.72                                   
REMARK 500 14 THR A   6     -174.63    -54.38                                   
REMARK 500 14 ALA A   8      -64.59    -90.13                                   
REMARK 500 14 SER A  17      106.34   -170.83                                   
REMARK 500 14 PRO A  21     -160.57    -69.76                                   
REMARK 500 14 ARG A  28      -77.97    -82.08                                   
REMARK 500 14 ILE A  30      -71.86    -63.52                                   
REMARK 500 14 ASN A  31      -19.29    -48.27                                   
REMARK 500 14 ASN A  41      119.51   -175.21                                   
REMARK 500 14 GLN A  58      107.76    -46.41                                   
REMARK 500 14 PHE A 106     -174.85    -61.92                                   
REMARK 500 14 PRO A 116       10.73    -69.85                                   
REMARK 500 14 THR A 131     -178.72   -171.67                                   
REMARK 500 15 ALA A   8      -72.02    -82.91                                   
REMARK 500 15 SER A  17      111.12   -163.23                                   
REMARK 500 15 PRO A  21     -161.81    -69.76                                   
REMARK 500 15 CYS A  23      -26.73    -38.19                                   
REMARK 500 15 ARG A  28      -76.05    -82.75                                   
REMARK 500 15 ILE A  30      -71.82    -64.21                                   
REMARK 500 15 GLN A  40      132.65   -175.30                                   
REMARK 500 15 ASN A  41      105.12   -160.36                                   
REMARK 500 15 GLN A  58      107.93    -46.46                                   
REMARK 500 15 PHE A 106     -175.28    -67.94                                   
REMARK 500 15 PRO A 116        3.83    -69.72                                   
REMARK 500 15 THR A 131     -179.07   -171.43                                   
REMARK 500 16 PRO A  21     -158.96    -69.69                                   
REMARK 500 16 CYS A  23      -44.48    -25.66                                   
REMARK 500 16 ASN A  32       -9.71    -58.59                                   
REMARK 500 16 GLN A  40      145.39   -175.47                                   
REMARK 500 16 ARG A  45       77.98    -65.70                                   
REMARK 500 16 GLN A  58      107.73    -46.37                                   
REMARK 500 16 ASN A  69       68.67   -119.30                                   
REMARK 500 16 GLN A  91       60.95   -118.32                                   
REMARK 500 16 ASN A  92      108.44   -165.98                                   
REMARK 500 16 PRO A 116        3.62    -69.74                                   
REMARK 500 16 THR A 131     -175.36   -171.18                                   
REMARK 500 17 SER A  17      109.41   -163.43                                   
REMARK 500 17 PRO A  21     -158.71    -69.71                                   
REMARK 500 17 CYS A  23      -42.93    -29.52                                   
REMARK 500 17 GLN A  58      107.80    -46.49                                   
REMARK 500 17 ASN A  92      105.64   -169.09                                   
REMARK 500 17 PRO A 116        4.53    -69.75                                   
REMARK 500 18 THR A   6     -174.11    -66.07                                   
REMARK 500 18 ALA A   8      -64.07    -90.39                                   
REMARK 500 18 SER A  17      105.43   -171.54                                   
REMARK 500 18 PRO A  21     -162.85    -69.70                                   
REMARK 500 18 CYS A  23      -30.13    -35.94                                   
REMARK 500 18 ILE A  30      -72.67    -73.29                                   
REMARK 500 18 ARG A  45       78.55    -69.46                                   
REMARK 500 18 GLN A  58      105.77    -46.41                                   
REMARK 500 18 PRO A 116        3.30    -69.77                                   
REMARK 500 19 THR A   6     -175.11    -61.58                                   
REMARK 500 19 PRO A  21     -159.73    -69.73                                   
REMARK 500 19 ARG A  28      -75.76    -85.92                                   
REMARK 500 19 ILE A  30      -72.46    -64.44                                   
REMARK 500 19 ASN A  31      -19.23    -47.77                                   
REMARK 500 19 GLN A  40      128.09   -175.06                                   
REMARK 500 19 GLN A  58       99.68    -46.54                                   
REMARK 500 19 SER A  74      155.77    -48.48                                   
REMARK 500 19 ASN A  95       42.35    -98.23                                   
REMARK 500 19 PHE A 106     -174.51    -68.03                                   
REMARK 500 19 PRO A 116        4.46    -69.71                                   
REMARK 500 19 THR A 131     -175.73   -173.14                                   
REMARK 500 20 ALA A   8      -72.04    -89.66                                   
REMARK 500 20 PRO A  21     -160.74    -69.71                                   
REMARK 500 20 ILE A  30      -71.58    -66.41                                   
REMARK 500 20 ASN A  31      -18.05    -46.99                                   
REMARK 500 20 ARG A  45       78.03    -65.78                                   
REMARK 500 20 GLN A  58      108.09    -46.54                                   
REMARK 500 20 THR A  67      -52.41   -134.33                                   
REMARK 500 20 SER A  74      166.62    -43.65                                   
REMARK 500 20 GLN A  91       61.10   -118.25                                   
REMARK 500 20 ASN A 113       93.10    -53.86                                   
REMARK 500 20 PRO A 116        3.01    -69.73                                   
REMARK 500 20 THR A 131     -175.22   -172.74                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 15717   RELATED DB: BMRB                                 
DBREF  2KB5 A    1   133  UNP    P12724   ECP_HUMAN       28    160             
SEQADV 2KB5 ARG A   97  UNP  P12724    THR   124 ENGINEERED                     
SEQRES   1 A  133  ARG PRO PRO GLN PHE THR ARG ALA GLN TRP PHE ALA ILE          
SEQRES   2 A  133  GLN HIS ILE SER LEU ASN PRO PRO ARG CYS THR ILE ALA          
SEQRES   3 A  133  MET ARG ALA ILE ASN ASN TYR ARG TRP ARG CYS LYS ASN          
SEQRES   4 A  133  GLN ASN THR PHE LEU ARG THR THR PHE ALA ASN VAL VAL          
SEQRES   5 A  133  ASN VAL CYS GLY ASN GLN SER ILE ARG CYS PRO HIS ASN          
SEQRES   6 A  133  ARG THR LEU ASN ASN CYS HIS ARG SER ARG PHE ARG VAL          
SEQRES   7 A  133  PRO LEU LEU HIS CYS ASP LEU ILE ASN PRO GLY ALA GLN          
SEQRES   8 A  133  ASN ILE SER ASN CYS ARG TYR ALA ASP ARG PRO GLY ARG          
SEQRES   9 A  133  ARG PHE TYR VAL VAL ALA CYS ASP ASN ARG ASP PRO ARG          
SEQRES  10 A  133  ASP SER PRO ARG TYR PRO VAL VAL PRO VAL HIS LEU ASP          
SEQRES  11 A  133  THR THR ILE                                                  
HELIX    1   1 ALA A    8  ILE A   16  1                                   9    
HELIX    2   2 ARG A   22  MET A   27  1                                   6    
HELIX    3   3 ILE A   30  ARG A   34  5                                   5    
HELIX    4   4 THR A   47  ASN A   53  1                                   7    
HELIX    5   5 VAL A   54  ASN A   57  5                                   4    
SHEET    1   A 3 GLN A  40  LEU A  44  0                                        
SHEET    2   A 3 VAL A  78  ASP A  84 -1  O  CYS A  83   N  ASN A  41           
SHEET    3   A 3 ARG A 101  ARG A 105 -1  O  GLY A 103   N  LEU A  80           
SHEET    1   B 3 CYS A  71  ARG A  73  0                                        
SHEET    2   B 3 VAL A 108  ASN A 113 -1  O  VAL A 109   N  HIS A  72           
SHEET    3   B 3 VAL A 124  LEU A 129 -1  O  HIS A 128   N  ALA A 110           
SSBOND   1 CYS A   23    CYS A   83                          1555   1555  1.98  
SSBOND   2 CYS A   37    CYS A   96                          1555   1555  2.10  
SSBOND   3 CYS A   55    CYS A  111                          1555   1555  2.05  
SSBOND   4 CYS A   62    CYS A   71                          1555   1555  2.11  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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