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Database: PDB
Entry: 2KPK
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Original site: 2KPK 
HEADER    PROTEIN BINDING                         16-OCT-09   2KPK              
TITLE     MAGI-1 PDZ1                                                           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-   
COMPND   3 CONTAINING PROTEIN 1;                                                
COMPND   4 CHAIN: A;                                                            
COMPND   5 FRAGMENT: UNP RESIDUES 455-580;                                      
COMPND   6 SYNONYM: BAI1-ASSOCIATED PROTEIN 1, BAP-1, MEMBRANE-ASSOCIATED       
COMPND   7 GUANYLATE KINASE INVERTED 1, MAGI-1, ATROPHIN-1-INTERACTING PROTEIN  
COMPND   8 3, AIP3, WW DOMAIN-CONTAINING PROTEIN 3, WWP3, TRINUCLEOTIDE REPEAT- 
COMPND   9 CONTAINING GENE 19 PROTEIN;                                          
COMPND  10 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: MAGI1, BAIAP1, BAP1, TNRC19;                                   
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PETM-41                                   
KEYWDS    PDZ DOMAIN, ATP-BINDING, CELL JUNCTION, CELL MEMBRANE, MEMBRANE,      
KEYWDS   2 NUCLEOTIDE-BINDING, PHOSPHOPROTEIN, TIGHT JUNCTION, PROTEIN BINDING  
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    S.CHARBONNIER,Y.NOMINE,J.RAMIREZ,K.LUCK,R.H.STOTE,G.TRAVE,B.KIEFFER,  
AUTHOR   2 R.A.ATKINSON                                                         
REVDAT   2   02-FEB-11 2KPK    1       JRNL                                     
REVDAT   1   27-OCT-10 2KPK    0                                                
JRNL        AUTH   S.CHARBONNIER,Y.NOMINE,J.RAMIREZ,K.LUCK,A.CHAPELLE,          
JRNL        AUTH 2 R.H.STOTE,G.TRAVE,B.KIEFFER,R.A.ATKINSON                     
JRNL        TITL   THE STRUCTURAL AND DYNAMIC RESPONSE OF MAGI-1 PDZ1 WITH      
JRNL        TITL 2 NON-CANONICAL DOMAIN BOUNDARIES TO BINDING OF HUMAN          
JRNL        TITL 3 PAPILLOMAVIRUS (HPV) E6                                      
JRNL        REF    J.MOL.BIOL.                                2011              
JRNL        REFN                   ESSN 1089-8638                               
JRNL        PMID   21238461                                                     
JRNL        DOI    10.1016/J.JMB.2011.01.015                                    
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   S.CHARBONNIER,M.A.COUTOULY,B.KIEFFER,G.TRAVE,R.A.ATKINSON    
REMARK   1  TITL   13C, 15N AND 1H RESONANCE ASSIGNMENT OF THE PDZ1 DOMAIN OF   
REMARK   1  TITL 2 MAGI-1 USING QUASI                                           
REMARK   1  REF    J.BIOMOL.NMR                  V.36 S    33 2006              
REMARK   1  REFN                   ISSN 0925-2738                               
REMARK   1  PMID   16636753                                                     
REMARK   1  DOI    10.1007/S10858-006-0017-7                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR_NIH                                           
REMARK   3   AUTHORS     : SCHWIETERS, KUSZEWSKI, TJANDRA, CLORE                
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NMR_WATERREFINE PROTOCOL                  
REMARK   4                                                                      
REMARK   4 2KPK COMPLIES WITH FORMAT V. 3.20, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 21-OCT-09.                  
REMARK 100 THE RCSB ID CODE IS RCSB101413.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 295                                
REMARK 210  PH                             : 6.8                                
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.2-0.6MM MAGI-1 PDZ1-1, 0.02-     
REMARK 210                                   0.10MM SODIUM PHOSPHATE-2, 50MM    
REMARK 210                                   SODIUM CHLORIDE-3, 2MM DTT-4, 90%  
REMARK 210                                   H2O/10% D2O; 0.2-0.6MM [U-15N]     
REMARK 210                                   MAGI-1 PDZ1-5, 0.02-0.10MM SODIUM  
REMARK 210                                   PHOSPHATE-6, 50MM SODIUM CHLORIDE  
REMARK 210                                   -7, 2MM DTT-8, 90% H2O/10% D2O;    
REMARK 210                                   0.2-0.6MM [U-100% 13C; U-100%      
REMARK 210                                   15N] MAGI-1 PDZ1-9, 0.02-0.10MM    
REMARK 210                                   SODIUM PHOSPHATE-10, 50MM SODIUM   
REMARK 210                                   CHLORIDE-11, 2MM DTT-12, 90% H2O/  
REMARK 210                                   10% D2O                            
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D HN(CO)CA; 3D HNCA; 3D HN(CO)    
REMARK 210                                   CACB; 3D HNCACB; 3D HNCO; 3D HCCH  
REMARK 210                                   -TOCSY; 3D HCCH-COSY; 3D 1H-15N    
REMARK 210                                   NOESY; 3D 1H-15N TOCSY; 3D 1H-13C  
REMARK 210                                   NOESY; 2D 1H-1H TOCSY; 2D 1H-1H    
REMARK 210                                   NOESY; 2D 15N T1; 2D 15N T2; 2D    
REMARK 210                                   1H-15N NOE                         
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ; 800 MHZ                   
REMARK 210  SPECTROMETER MODEL             : DRX                                
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR, NMRPIPE, XEASY CARA,      
REMARK 210                                   ATNOS-CANDID                       
REMARK 210   METHOD USED                   : MOLECULAR DYNAMICS                 
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 64                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST ENERGY  
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   HE2  LYS A    14     H    ARG A    99              1.56            
REMARK 500   HZ1  LYS A     1     OE2  GLU A    36              1.59            
REMARK 500   OD2  ASP A   106     HG1  THR A   112              1.63            
REMARK 500   HG1  THR A    18     OD2  ASP A    55              1.66            
REMARK 500   OD1  ASP A   122     HZ3  LYS A   123              1.67            
REMARK 500   O    GLY A    34     H    HIS A    77              1.68            
REMARK 500   HZ1  LYS A    22     O    ILE A    87              1.69            
REMARK 500   O    VAL A    63     H    CYS A    98              1.70            
REMARK 500   O    GLU A    36     H    ASP A    38              1.71            
REMARK 500   HZ2  LYS A     1     O    ASP A    38              1.72            
REMARK 500   O    PRO A    51     H    ASP A    55              1.73            
REMARK 500   HG   SER A    45     OD2  ASP A   108              1.73            
REMARK 500   H    VAL A    63     O    CYS A    98              1.74            
REMARK 500   HZ1  LYS A    14     OD2  ASP A    62              1.75            
REMARK 500   HZ2  LYS A    82     OG   SER A    86              1.75            
REMARK 500   HA   LYS A    19     O    VAL A    93              1.76            
REMARK 500   HZ3  LYS A    12     OH   TYR A   101              1.77            
REMARK 500   O    VAL A    65     HG   SER A    66              1.77            
REMARK 500   H    LYS A     1     OE1  GLU A    39              1.77            
REMARK 500   H    GLY A    34     O    PHE A    40              1.78            
REMARK 500   OD1  ASP A    62    HH11  ARG A    99              1.78            
REMARK 500   O    THR A    18     H    LEU A    95              1.78            
REMARK 500   H    LEU A    20     O    VAL A    93              1.79            
REMARK 500   HZ1  LYS A    19     OD1  ASP A    94              1.80            
REMARK 500   O    SER A    66     H    GLU A    96              1.80            
REMARK 500   HA   PRO A    37     O    GLY A    74              1.80            
REMARK 500   O    VAL A    32     H    GLN A    42              1.80            
REMARK 500   O    GLU A    36     H    GLU A    39              1.81            
REMARK 500   OD1  ASP A   122     H    GLU A   124              1.81            
REMARK 500   HG1  THR A    30     O    SER A    45              1.81            
REMARK 500   HZ   PHE A     4     O    LEU A    73              1.82            
REMARK 500   H    VAL A    65     O    GLU A    96              1.83            
REMARK 500   OD1  ASN A     7     H    SER A     9              1.84            
REMARK 500   O    LYS A    82     HG   SER A    86              1.84            
REMARK 500   H    SER A    23     O    GLY A    26              1.85            
REMARK 500   H    VAL A    32     O    GLN A    42              1.85            
REMARK 500   OD2  ASP A    55     HB2  LYS A    57              1.86            
REMARK 500   O    GLY A    28     H    VAL A    47              1.86            
REMARK 500   H    ILE A    16     O    LEU A    97              1.87            
REMARK 500   O    LYS A    14     H    ARG A    99              1.87            
REMARK 500   H    VAL A    67     O    THR A    70              1.88            
REMARK 500   O    VAL A    47     H    GLY A    50              1.88            
REMARK 500   H    ILE A    43     O    ASP A    62              1.88            
REMARK 500   O    THR A    76     H    VAL A    80              1.89            
REMARK 500  HH21  ARG A     6     OD1  ASP A    38              1.89            
REMARK 500   O    ILE A    16     H    LEU A    97              1.90            
REMARK 500   O    VAL A    67     H    THR A    70              1.91            
REMARK 500   H    THR A    30     O    SER A    45              1.91            
REMARK 500   HZ3  LYS A     1     OD2  ASP A    38              1.91            
REMARK 500   O    VAL A    81     H    GLN A    85              1.92            
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS    1849 CLOSE CONTACTS                                
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500  5 GLY A  34   CA    GLY A  34   C      -0.104                       
REMARK 500  7 GLY A  34   CA    GLY A  34   C      -0.106                       
REMARK 500  9 GLY A  34   CA    GLY A  34   C      -0.108                       
REMARK 500 16 GLY A  34   CA    GLY A  34   C      -0.106                       
REMARK 500 17 GLY A  34   CA    GLY A  34   C      -0.119                       
REMARK 500 18 GLY A  34   CA    GLY A  34   C      -0.104                       
REMARK 500 19 GLY A  34   CA    GLY A  34   C      -0.119                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  4 PRO A 110   CB  -  CA  -  C   ANGL. DEV. = -12.8 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 MET A  -1       57.51   -110.15                                   
REMARK 500  1 LYS A   1       71.57     47.94                                   
REMARK 500  1 ARG A   6        6.23     55.21                                   
REMARK 500  1 LYS A  22       97.52    -69.64                                   
REMARK 500  1 SER A  23     -155.21    -83.59                                   
REMARK 500  1 PRO A  37       52.83    -68.30                                   
REMARK 500  1 ASP A  38        3.28    171.19                                   
REMARK 500  1 SER A  45      173.13    161.77                                   
REMARK 500  1 ASN A 111       -7.62    -54.75                                   
REMARK 500  2 ALA A  -2       23.29   -152.40                                   
REMARK 500  2 ARG A   6        5.59     54.32                                   
REMARK 500  2 LYS A  22       96.78    -65.76                                   
REMARK 500  2 SER A  23     -154.79    -90.00                                   
REMARK 500  2 PRO A  37       58.96    -67.27                                   
REMARK 500  2 ASP A  38       -9.53    174.40                                   
REMARK 500  2 SER A  45      173.41    161.14                                   
REMARK 500  2 ASN A  68      -82.23     65.68                                   
REMARK 500  2 ASP A  69       33.65   -163.96                                   
REMARK 500  2 PRO A 104     -150.57    -70.52                                   
REMARK 500  2 ASN A 111       -8.81    -59.51                                   
REMARK 500  2 VAL A 115       70.82     65.04                                   
REMARK 500  2 SER A 117      115.45     69.46                                   
REMARK 500  2 ALA A 119      111.96   -171.69                                   
REMARK 500  2 ASP A 122      134.34     78.60                                   
REMARK 500  2 LYS A 123       42.81   -141.16                                   
REMARK 500  3 ALA A  -2      -92.13     63.20                                   
REMARK 500  3 LYS A   1       96.84     61.56                                   
REMARK 500  3 ARG A   6        6.47     55.25                                   
REMARK 500  3 LYS A  22       97.52    -62.71                                   
REMARK 500  3 SER A  23     -157.24    -81.69                                   
REMARK 500  3 PRO A  37       58.18    -66.56                                   
REMARK 500  3 ASP A  38      -11.92    177.08                                   
REMARK 500  3 SER A  45      174.40    165.15                                   
REMARK 500  3 ASP A  69       -8.12     77.93                                   
REMARK 500  3 ALA A  91     -169.99   -101.77                                   
REMARK 500  3 PRO A 104      -74.59    -72.64                                   
REMARK 500  3 ASN A 111        6.40    -62.99                                   
REMARK 500  3 THR A 116     -158.02   -106.53                                   
REMARK 500  3 ALA A 119       43.09    -88.46                                   
REMARK 500  3 LYS A 123       32.18    -86.84                                   
REMARK 500  4 LYS A   1       70.85     55.66                                   
REMARK 500  4 ARG A   6        4.54     53.03                                   
REMARK 500  4 LYS A  22       97.79    -59.54                                   
REMARK 500  4 SER A  23     -155.30    -84.82                                   
REMARK 500  4 PRO A  37       64.04    -66.72                                   
REMARK 500  4 ASP A  38       -9.77    170.69                                   
REMARK 500  4 SER A  45      172.71    173.20                                   
REMARK 500  4 PRO A 104      158.65    -42.05                                   
REMARK 500  4 LEU A 114       36.17    -81.62                                   
REMARK 500  4 VAL A 115       40.57   -155.42                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS     275 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 HIS A   75     THR A   76          6       149.99                    
REMARK 500 HIS A   75     THR A   76          7       148.91                    
REMARK 500 HIS A   75     THR A   76          9       148.68                    
REMARK 500 HIS A   75     THR A   76         16       150.00                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 ARG A  99         0.12    SIDE CHAIN                              
REMARK 500  2 ARG A  99         0.14    SIDE CHAIN                              
REMARK 500  3 ARG A  99         0.10    SIDE CHAIN                              
REMARK 500  4 ARG A  99         0.08    SIDE CHAIN                              
REMARK 500  5 ARG A  99         0.09    SIDE CHAIN                              
REMARK 500  6 ARG A  99         0.09    SIDE CHAIN                              
REMARK 500  9 ARG A  99         0.09    SIDE CHAIN                              
REMARK 500 11 ARG A  99         0.08    SIDE CHAIN                              
REMARK 500 12 ARG A  99         0.09    SIDE CHAIN                              
REMARK 500 13 ARG A  99         0.08    SIDE CHAIN                              
REMARK 500 18 ARG A  99         0.12    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 2KPL   RELATED DB: PDB                                   
REMARK 900 MAGI-1 PDZ1 / E6CT COMPLEX                                           
DBREF  2KPK A    0   125  UNP    Q96QZ7   MAGI1_HUMAN    455    580             
SEQADV 2KPK GLY A   -3  UNP  Q96QZ7              EXPRESSION TAG                 
SEQADV 2KPK ALA A   -2  UNP  Q96QZ7              EXPRESSION TAG                 
SEQADV 2KPK MET A   -1  UNP  Q96QZ7              EXPRESSION TAG                 
SEQRES   1 A  129  GLY ALA MET GLY LYS PRO PHE PHE THR ARG ASN PRO SER          
SEQRES   2 A  129  GLU LEU LYS GLY LYS PHE ILE HIS THR LYS LEU ARG LYS          
SEQRES   3 A  129  SER SER ARG GLY PHE GLY PHE THR VAL VAL GLY GLY ASP          
SEQRES   4 A  129  GLU PRO ASP GLU PHE LEU GLN ILE LYS SER LEU VAL LEU          
SEQRES   5 A  129  ASP GLY PRO ALA ALA LEU ASP GLY LYS MET GLU THR GLY          
SEQRES   6 A  129  ASP VAL ILE VAL SER VAL ASN ASP THR CYS VAL LEU GLY          
SEQRES   7 A  129  HIS THR HIS ALA GLN VAL VAL LYS ILE PHE GLN SER ILE          
SEQRES   8 A  129  PRO ILE GLY ALA SER VAL ASP LEU GLU LEU CYS ARG GLY          
SEQRES   9 A  129  TYR PRO LEU PRO PHE ASP PRO ASP ASP PRO ASN THR SER          
SEQRES  10 A  129  LEU VAL THR SER VAL ALA ILE LEU ASP LYS GLU PRO              
HELIX    1   1 GLY A   50  GLY A   56  1                                   7    
HELIX    2   2 THR A   76  ILE A   87  1                                  12    
SHEET    1   A 5 LYS A  14  LYS A  22  0                                        
SHEET    2   A 5 ALA A  91  ARG A  99 -1  O  LEU A  95   N  THR A  18           
SHEET    3   A 5 VAL A  63  VAL A  67 -1  N  VAL A  63   O  CYS A  98           
SHEET    4   A 5 GLU A  39  LEU A  46 -1  N  LEU A  41   O  ILE A  64           
SHEET    5   A 5 PHE A  29  GLU A  36 -1  N  GLU A  36   O  GLU A  39           
SHEET    1   B 4 LYS A  14  LYS A  22  0                                        
SHEET    2   B 4 ALA A  91  ARG A  99 -1  O  LEU A  95   N  THR A  18           
SHEET    3   B 4 VAL A  63  VAL A  67 -1  N  VAL A  63   O  CYS A  98           
SHEET    4   B 4 THR A  70  CYS A  71 -1  O  THR A  70   N  VAL A  67           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system