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Database: PDB
Entry: 2MH0
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Original site: 2MH0 
HEADER    TRANSCRIPTION/TRANSFERASE               12-NOV-13   2MH0              
TITLE     SOLUTION NMR STRUCTURE OF THE P300 TAZ2:ETAD1 COMPLEX                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TRANSCRIPTION FACTOR E2-ALPHA;                             
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: ACTIVATION DOMAIN 1 (ETAD1), E2A RESIDUES 1-37;            
COMPND   5 SYNONYM: CLASS B BASIC HELIX-LOOP-HELIX PROTEIN 21, BHLHB21,         
COMPND   6 IMMUNOGLOBULIN ENHANCER-BINDING FACTOR E12/E47, IMMUNOGLOBULIN       
COMPND   7 TRANSCRIPTION FACTOR 1, KAPPA-E2-BINDING FACTOR, TRANSCRIPTION FACTOR
COMPND   8 3, TCF-3, TRANSCRIPTION FACTOR ITF-1;                                
COMPND   9 ENGINEERED: YES;                                                     
COMPND  10 MOL_ID: 2;                                                           
COMPND  11 MOLECULE: HISTONE ACETYLTRANSFERASE P300;                            
COMPND  12 CHAIN: B;                                                            
COMPND  13 FRAGMENT: TAZ-TYPE 2 ZINC FINGER RESIDUES 1723-1812;                 
COMPND  14 SYNONYM: P300 HAT, E1A-ASSOCIATED PROTEIN P300;                      
COMPND  15 EC: 2.3.1.48;                                                        
COMPND  16 ENGINEERED: YES;                                                     
COMPND  17 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: TCF3, BHLHB21, E2A, ITF1;                                      
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR: PET21;                                     
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  11 ORGANISM_COMMON: HUMAN;                                              
SOURCE  12 ORGANISM_TAXID: 9606;                                                
SOURCE  13 GENE: EP300, P300;                                                   
SOURCE  14 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  15 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  16 EXPRESSION_SYSTEM_VECTOR: PET21                                      
KEYWDS    TRANSCRIPTION-TRANSFERASE COMPLEX                                     
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    D.N.LANGELAAN,S.P.SMITH,S.CHITAYAT                                    
REVDAT   1   12-NOV-14 2MH0    0                                                
JRNL        AUTH   D.LANGELAAN,S.SMITH,S.CHITAYAT                               
JRNL        TITL   SOLUTION NMR STRUCTURE OF THE P300 TAZ2:ETAD1 COMPLEX        
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS, CYANA 2.1                                       
REMARK   3   AUTHORS     : BRUNGER, ADAMS, CLORE, GROS, NILGES AND READ (CNS)   
REMARK   3                 , GUNTERT, MUMENTHALER AND WUTHRICH (CYANA)          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2MH0 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 09-JAN-14.                  
REMARK 100 THE RCSB ID CODE IS RCSB103606.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 288                                
REMARK 210  PH                             : 6.5                                
REMARK 210  IONIC STRENGTH                 : 0                                  
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.4 MM [U-99% 13C; U-99% 15N]      
REMARK 210                                   ETAD1, 2 MM TAZ2, 20 MM MES, 1 MM  
REMARK 210                                   SODIUM AZIDE, 5 MM BETA-           
REMARK 210                                   MERCAPTOETHANOL, 90% H2O/10% D2O;  
REMARK 210                                   3078 UM ETAD1, 1038 UM [U-99%      
REMARK 210                                   13C; U-99% 15N] TAZ2, 20 MM MES,   
REMARK 210                                   1 MM SODIUM AZIDE, 5 MM BETA-      
REMARK 210                                   MERCAPTOETHANOL, 90% H2O/10% D2O   
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D 1H-15N HSQC; 3D CBCA(CO)NH;     
REMARK 210                                   3D HNCACB; 3D HNCO; 3D HCACO; 3D   
REMARK 210                                   1H-15N NOESY; 3D HCCH-TOCSY; 3D    
REMARK 210                                   1H-13C NOESY ALIPHATIC; 3D 1H-13C  
REMARK 210                                   NOESY AROMATIC                     
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ; 500 MHZ; 800 MHZ          
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NMRPIPE, VNMRJ, ANALYSIS 3,        
REMARK 210                                   CYANA 2.1                          
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST ENERGY  
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 MET A   7      166.54     68.14                                   
REMARK 500  1 LEU A  28      168.82     61.77                                   
REMARK 500  1 LYS A  34       95.91     61.89                                   
REMARK 500  1 GLN B1725     -179.12     64.47                                   
REMARK 500  2 THR A  12      -63.69   -132.25                                   
REMARK 500  2 LEU A  28      161.02     61.72                                   
REMARK 500  2 ARG A  36      157.49     68.25                                   
REMARK 500  2 SER B1722      -70.95   -137.34                                   
REMARK 500  2 ALA B1723       39.52   -144.34                                   
REMARK 500  3 ASN A   2       78.35     53.30                                   
REMARK 500  3 PRO A  27      -82.77    -76.64                                   
REMARK 500  3 LEU A  28      164.49     61.23                                   
REMARK 500  3 THR A  31       17.34     52.47                                   
REMARK 500  3 ASN A  32     -141.45     53.23                                   
REMARK 500  3 LYS A  34       92.36   -168.15                                   
REMARK 500  3 SER B1722      136.91     68.79                                   
REMARK 500  4 MET A   1      -66.92     71.08                                   
REMARK 500  4 ARG A   6      132.77   -175.22                                   
REMARK 500  4 MET A   7       97.80    -54.08                                   
REMARK 500  4 PRO A   9        6.85    -61.96                                   
REMARK 500  4 THR A  12      -57.91   -132.45                                   
REMARK 500  4 LEU A  28      162.42     61.99                                   
REMARK 500  4 ASN A  32       13.95     52.43                                   
REMARK 500  4 GLN B1725      131.09     68.85                                   
REMARK 500  4 LYS B1800       42.76    -89.43                                   
REMARK 500  5 ASN A   2      100.90    -56.76                                   
REMARK 500  5 GLN A   5      -86.16     59.85                                   
REMARK 500  5 ARG A   6     -169.53    179.56                                   
REMARK 500  5 THR A  12      -45.84   -141.16                                   
REMARK 500  5 PRO A  27      -74.76    -76.39                                   
REMARK 500  5 LEU A  28      144.71     62.43                                   
REMARK 500  5 ASN A  32      109.47     67.69                                   
REMARK 500  5 LYS A  34      -66.52     73.73                                   
REMARK 500  5 ARG A  36       75.73     56.70                                   
REMARK 500  5 ALA B1723       95.65    -64.37                                   
REMARK 500  5 LYS B1800       43.98   -102.53                                   
REMARK 500  6 GLN A   5     -170.83     63.04                                   
REMARK 500  6 PRO A  27      -70.84    -79.98                                   
REMARK 500  6 LEU A  28      153.51     64.87                                   
REMARK 500  6 THR A  31      -81.40     54.53                                   
REMARK 500  6 GLN B1725     -178.47     65.98                                   
REMARK 500  7 ARG A   6      146.67     71.97                                   
REMARK 500  7 MET A   7       96.43    -56.51                                   
REMARK 500  7 PRO A  27      -72.42    -79.42                                   
REMARK 500  7 LEU A  28      165.20     63.19                                   
REMARK 500  7 PRO A  29     -176.28    -69.66                                   
REMARK 500  7 ASN A  32      102.07     66.24                                   
REMARK 500  7 ARG A  36      108.85     65.65                                   
REMARK 500  7 ALA B1723       85.22     62.29                                   
REMARK 500  7 GLN B1725      167.56     67.92                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS     148 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 19610   RELATED DB: BMRB                                 
DBREF  2MH0 A    1    37  UNP    P15923   TFE2_HUMAN       1     37             
DBREF  2MH0 B 1723  1812  UNP    Q09472   EP300_HUMAN   1723   1812             
SEQADV 2MH0 GLY A   -1  UNP  P15923              EXPRESSION TAG                 
SEQADV 2MH0 SER A    0  UNP  P15923              EXPRESSION TAG                 
SEQADV 2MH0 GLY B 1721  UNP  Q09472              EXPRESSION TAG                 
SEQADV 2MH0 SER B 1722  UNP  Q09472              EXPRESSION TAG                 
SEQADV 2MH0 ALA B 1738  UNP  Q09472    CYS  1738 ENGINEERED MUTATION            
SEQADV 2MH0 ALA B 1746  UNP  Q09472    CYS  1746 ENGINEERED MUTATION            
SEQADV 2MH0 ALA B 1789  UNP  Q09472    CYS  1789 ENGINEERED MUTATION            
SEQADV 2MH0 ALA B 1790  UNP  Q09472    CYS  1790 ENGINEERED MUTATION            
SEQRES   1 A   39  GLY SER MET ASN GLN PRO GLN ARG MET ALA PRO VAL GLY          
SEQRES   2 A   39  THR ASP LYS GLU LEU SER ASP LEU LEU ASP PHE SER MET          
SEQRES   3 A   39  MET PHE PRO LEU PRO VAL THR ASN GLY LYS GLY ARG PRO          
SEQRES   1 B   92  GLY SER ALA THR GLN SER PRO GLY ASP SER ARG ARG LEU          
SEQRES   2 B   92  SER ILE GLN ARG ALA ILE GLN SER LEU VAL HIS ALA ALA          
SEQRES   3 B   92  GLN CYS ARG ASN ALA ASN CYS SER LEU PRO SER CYS GLN          
SEQRES   4 B   92  LYS MET LYS ARG VAL VAL GLN HIS THR LYS GLY CYS LYS          
SEQRES   5 B   92  ARG LYS THR ASN GLY GLY CYS PRO ILE CYS LYS GLN LEU          
SEQRES   6 B   92  ILE ALA LEU ALA ALA TYR HIS ALA LYS HIS CYS GLN GLU          
SEQRES   7 B   92  ASN LYS CYS PRO VAL PRO PHE CYS LEU ASN ILE LYS GLN          
SEQRES   8 B   92  LYS                                                          
HELIX    1   1 GLY A   11  PHE A   26  1                                  16    
HELIX    2   2 SER B 1726  CYS B 1748  1                                  23    
HELIX    3   3 LEU B 1755  GLY B 1770  1                                  16    
HELIX    4   4 CYS B 1779  CYS B 1796  1                                  18    
HELIX    5   5 VAL B 1803  GLN B 1811  1                                   9    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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