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Database: PDB
Entry: 2YUO
LinkDB: 2YUO
Original site: 2YUO 
HEADER    SIGNALING PROTEIN                       06-APR-07   2YUO              
TITLE     SOLUTION STRUCTURE OF THE SH3 DOMAIN OF MOUSE RUN AND TBC1            
TITLE    2 DOMAIN CONTAINING 3                                                  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: RUN AND TBC1 DOMAIN CONTAINING 3;                          
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: SH3 DOMAIN;                                                
COMPND   5 SYNONYM: CIP85;                                                      
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: HOUSE MOUSE;                                        
SOURCE   4 ORGANISM_TAXID: 10090;                                               
SOURCE   5 GENE: RUTBC3;                                                        
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P060417-23;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS                           
KEYWDS    CIP85, STRUCTURAL GENOMICS, NPPSFA, NATIONAL PROJECT ON               
KEYWDS   2 PROTEIN STRUCTURAL AND FUNCTIONAL ANALYSES, RIKEN                    
KEYWDS   3 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE, RSGI, SIGNALING           
KEYWDS   4 PROTEIN                                                              
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    H.ABE,N.TOCHIO,K.MIYAMOTO,K.SAITO,A.SASAGAWA,S.KOSHIBA,               
AUTHOR   2 M.INOUE,T.KIGAWA,S.YOKOYAMA,RIKEN STRUCTURAL                         
AUTHOR   3 GENOMICS/PROTEOMICS INITIATIVE (RSGI)                                
REVDAT   2   24-FEB-09 2YUO    1       VERSN                                    
REVDAT   1   09-OCT-07 2YUO    0                                                
JRNL        AUTH   H.ABE,N.TOCHIO,K.MIYAMOTO,K.SAITO,A.SASAGAWA,                
JRNL        AUTH 2 S.KOSHIBA,M.INOUE,T.KIGAWA,S.YOKOYAMA                        
JRNL        TITL   SOLUTION STRUCTURE OF THE SH3 DOMAIN OF MOUSE RUN            
JRNL        TITL 2 AND TBC1 DOMAIN CONTAINING 3                                 
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.17                                         
REMARK   3   AUTHORS     : GUNTERT, P.                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2YUO COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 28-JUN-07.                  
REMARK 100 THE RCSB ID CODE IS RCSB027156.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 296                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.00MM PROTEIN; 20MM D-TRIS-       
REMARK 210                                   HCL; 100MM NACL; 1MM D-DTT;        
REMARK 210                                   0.02% NAN3 ;0.05MM ZNCL2, 1MM      
REMARK 210                                   IDA; 90% H2O, 10% D2O              
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 3.5, NMRPIPE               
REMARK 210                                   20031121, NMNMRVIEW 5.0.4,         
REMARK 210                                   KUJIRA 0.9747, CYANA 2.0.17        
REMARK 210   METHOD USED                   : NULL                               
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS, TARGET       
REMARK 210                                   FUNCTION                           
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASN A  29       34.23     73.29                                   
REMARK 500  1 ILE A  35      -63.92    -91.21                                   
REMARK 500  1 ASP A  63       78.76   -119.19                                   
REMARK 500  1 LYS A  67       54.33     34.60                                   
REMARK 500  1 TYR A  69      -58.41   -130.08                                   
REMARK 500  1 PRO A  75       86.65    -69.70                                   
REMARK 500  2 ILE A  35      -61.63    -92.42                                   
REMARK 500  2 SER A  66      107.12   -170.30                                   
REMARK 500  2 LYS A  67      104.29    -36.34                                   
REMARK 500  2 PRO A  75        2.54    -69.83                                   
REMARK 500  2 SER A  76       54.13     37.18                                   
REMARK 500  3 ARG A  18      133.10    -34.48                                   
REMARK 500  3 GLU A  64       43.21     36.51                                   
REMARK 500  3 LYS A  67      140.40   -174.83                                   
REMARK 500  4 SER A   2      171.23    -56.99                                   
REMARK 500  4 SER A  73      127.24    -35.05                                   
REMARK 500  4 SER A  76      133.43    -35.53                                   
REMARK 500  5 SER A   6      103.77    -39.25                                   
REMARK 500  5 ARG A  18      117.44    -39.36                                   
REMARK 500  5 PRO A  75       89.03    -69.75                                   
REMARK 500  6 GLU A  40      -27.29    -38.54                                   
REMARK 500  6 HIS A  41      -60.48    -93.52                                   
REMARK 500  6 ILE A  71       55.91   -113.81                                   
REMARK 500  6 PRO A  75       88.72    -69.67                                   
REMARK 500  7 SER A   3      -62.10   -103.29                                   
REMARK 500  7 ASP A  63       31.33    -88.65                                   
REMARK 500  7 GLU A  68       46.69    -78.63                                   
REMARK 500  8 SER A   2       50.11     74.40                                   
REMARK 500  8 ASP A  63       34.83    -91.86                                   
REMARK 500  8 GLU A  64       40.88     36.80                                   
REMARK 500  8 SER A  76      122.05    -39.60                                   
REMARK 500  9 SER A   6       43.57    -98.09                                   
REMARK 500  9 LYS A  38      -63.45    -90.47                                   
REMARK 500  9 ASN A  48       29.88     49.64                                   
REMARK 500  9 TYR A  69       89.18    -67.31                                   
REMARK 500  9 SER A  77      -60.04   -121.37                                   
REMARK 500 10 ARG A  18      108.79    -50.89                                   
REMARK 500 10 GLU A  23      154.19    -44.15                                   
REMARK 500 10 SER A  70      164.28    -45.45                                   
REMARK 500 10 ILE A  71       95.02    -47.48                                   
REMARK 500 11 SER A   5      169.62    -49.29                                   
REMARK 500 11 GLU A  68      121.98    -39.28                                   
REMARK 500 11 ALA A  72      145.69   -170.02                                   
REMARK 500 12 ARG A  18      127.60    -37.23                                   
REMARK 500 12 ILE A  35      -61.99    -94.34                                   
REMARK 500 12 SER A  66     -178.77    -55.65                                   
REMARK 500 12 SER A  70      108.39    -50.36                                   
REMARK 500 12 ALA A  72       92.35    -56.15                                   
REMARK 500 13 SER A   6       81.28    -68.24                                   
REMARK 500 13 ILE A  35      -64.15    -90.61                                   
REMARK 500 13 ASP A  63       32.64    -93.91                                   
REMARK 500 13 ARG A  65       49.97     39.11                                   
REMARK 500 13 SER A  70      -33.65   -133.50                                   
REMARK 500 14 SER A   2      -57.65   -121.39                                   
REMARK 500 14 SER A   3      109.28    -49.34                                   
REMARK 500 14 SER A   5      156.84    -41.11                                   
REMARK 500 14 ARG A  18      160.26    -47.05                                   
REMARK 500 14 ASP A  21      -26.79    -38.32                                   
REMARK 500 14 SER A  66      116.23   -174.03                                   
REMARK 500 14 GLU A  68       47.41    -79.30                                   
REMARK 500 14 ILE A  71      146.15    -39.35                                   
REMARK 500 14 SER A  77      142.63   -172.00                                   
REMARK 500 15 LYS A  38      -66.06    -99.63                                   
REMARK 500 15 SER A  66       97.81    -50.61                                   
REMARK 500 15 PRO A  75        2.19    -69.67                                   
REMARK 500 16 ARG A  18      121.36    -34.51                                   
REMARK 500 16 LEU A  47      133.30   -170.82                                   
REMARK 500 16 ASP A  63       37.06    -84.53                                   
REMARK 500 16 GLU A  64       42.43     34.40                                   
REMARK 500 16 ARG A  65       37.93    -83.73                                   
REMARK 500 17 SER A   6       98.21    -56.31                                   
REMARK 500 17 ARG A  18      153.61    -48.63                                   
REMARK 500 17 GLN A  37       79.52   -112.97                                   
REMARK 500 18 SER A   2      160.22    -44.51                                   
REMARK 500 18 GLU A  40      -26.03    -39.38                                   
REMARK 500 18 GLU A  64      172.29    -51.89                                   
REMARK 500 18 GLU A  68      107.82   -174.78                                   
REMARK 500 18 ALA A  72       95.50    -67.15                                   
REMARK 500 18 SER A  76      125.14    -34.60                                   
REMARK 500 19 ARG A  65       41.31     35.98                                   
REMARK 500 19 SER A  70      -33.67   -133.41                                   
REMARK 500 19 SER A  73      165.40    -46.00                                   
REMARK 500 19 SER A  77       42.20    -86.46                                   
REMARK 500 20 SER A   2       42.11     35.85                                   
REMARK 500 20 SER A   5       43.58   -102.92                                   
REMARK 500 20 LYS A  67      116.02    -34.33                                   
REMARK 500 20 SER A  73       31.14     38.73                                   
REMARK 500 20 SER A  76      107.84    -52.34                                   
REMARK 500 20 SER A  77      106.44    -42.95                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: MMI002015381.1   RELATED DB: TARGETDB                    
DBREF  2YUO A    8    72  UNP    Q8VCZ6   Q8VCZ6_MOUSE   483    547             
SEQADV 2YUO GLY A    1  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO SER A    2  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO SER A    3  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO GLY A    4  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO SER A    5  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO SER A    6  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO GLY A    7  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO SER A   73  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO GLY A   74  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO PRO A   75  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO SER A   76  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO SER A   77  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQADV 2YUO GLY A   78  UNP  Q8VCZ6              EXPRESSION TAG                 
SEQRES   1 A   78  GLY SER SER GLY SER SER GLY ARG ARG ALA LYS ALA LEU          
SEQRES   2 A   78  LEU ASP PHE GLU ARG HIS ASP ASP ASP GLU LEU GLY PHE          
SEQRES   3 A   78  ARG LYS ASN ASP ILE ILE THR ILE ILE SER GLN LYS ASP          
SEQRES   4 A   78  GLU HIS CYS TRP VAL GLY GLU LEU ASN GLY LEU ARG GLY          
SEQRES   5 A   78  TRP PHE PRO ALA LYS PHE VAL GLU VAL LEU ASP GLU ARG          
SEQRES   6 A   78  SER LYS GLU TYR SER ILE ALA SER GLY PRO SER SER GLY          
SHEET    1   A 5 LEU A  50  PRO A  55  0                                        
SHEET    2   A 5 CYS A  42  LEU A  47 -1  N  TRP A  43   O  PHE A  54           
SHEET    3   A 5 ILE A  31  SER A  36 -1  N  ILE A  35   O  VAL A  44           
SHEET    4   A 5 ARG A   8  ALA A  12 -1  N  ALA A  10   O  ILE A  32           
SHEET    5   A 5 VAL A  59  LEU A  62 -1  O  GLU A  60   N  LYS A  11           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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