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Database: PDB
Entry: 2Z0N
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Original site: 2Z0N 
HEADER    SIGNALING PROTEIN                       07-MAY-07   2Z0N              
TITLE     CRYSTAL STRUCTURE OF APPL1-BAR DOMAIN                                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DCC-INTERACTING PROTEIN 13-ALPHA;                          
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: REISDUES 1-275;                                            
COMPND   5 SYNONYM: APPL1, DIP13-ALPHA, ADAPTER PROTEIN CONTAINING PH           
COMPND   6 DOMAIN, PTB DOMAIN AND LEUCINE ZIPPER MOTIF 1;                       
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   7 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   8 EXPRESSION_SYSTEM_PLASMID: PGEX                                      
KEYWDS    HELIX BUNDLE, CELL CYCLE, COILED COIL, ENDOSOME, MEMBRANE,            
KEYWDS   2 NUCLEUS, PHOSPHORYLATION, SIGNALING PROTEIN, STRUCTURAL              
KEYWDS   3 GENOMICS, NPPSFA, NATIONAL PROJECT ON PROTEIN STRUCTURAL             
KEYWDS   4 AND FUNCTIONAL ANALYSES, RIKEN STRUCTURAL                            
KEYWDS   5 GENOMICS/PROTEOMICS INITIATIVE, RSGI                                 
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    K.MURAYAMA,M.KATO-MURAYAMA,A.SAKAMOTO,M.SHIROUZU,S.YOKOYAMA,          
AUTHOR   2 RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)               
REVDAT   2   24-FEB-09 2Z0N    1       VERSN                                    
REVDAT   1   13-MAY-08 2Z0N    0                                                
JRNL        AUTH   K.MURAYAMA,M.KATO-MURAYAMA,A.SAKAMOTO,M.SHIROUZU,            
JRNL        AUTH 2 S.YOKOYAMA                                                   
JRNL        TITL   CRYSTAL STRUCTURE OF APPL1-BAR DOMAIN                        
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.95 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.1                                              
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,             
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : NULL                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.95                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 35.52                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : 1385109.600                    
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : 0.0000                         
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 99.6                           
REMARK   3   NUMBER OF REFLECTIONS             : 19488                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.209                           
REMARK   3   FREE R VALUE                     : 0.259                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 9.900                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1920                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : 0.006                           
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 6                            
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.95                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.07                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 99.20                        
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : 2860                         
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2220                       
REMARK   3   BIN FREE R VALUE                    : 0.2750                       
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : 9.70                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : 306                          
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : 0.016                        
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 1911                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 96                                      
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 19.10                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 31.20                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 9.40000                                              
REMARK   3    B22 (A**2) : -6.23000                                             
REMARK   3    B33 (A**2) : -3.16000                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : 0.22                            
REMARK   3   ESD FROM SIGMAA              (A) : 0.08                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : 5.00                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : 0.28                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : 0.08                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.024                           
REMARK   3   BOND ANGLES            (DEGREES) : 1.90                            
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : 16.10                           
REMARK   3   IMPROPER ANGLES        (DEGREES) : 1.21                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : RESTRAINED                                
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : 2.020 ; 1.500                
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : 2.650 ; 2.000                
REMARK   3   SIDE-CHAIN BOND              (A**2) : 3.720 ; 2.000                
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : 5.280 ; 2.500                
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : FLAT MODEL                                           
REMARK   3   KSOL        : 0.38                                                 
REMARK   3   BSOL        : 43.91                                                
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM                              
REMARK   3  PARAMETER FILE  2  : WATER_REP.PARAM                                
REMARK   3  PARAMETER FILE  3  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : PROTEIN.TOP                                    
REMARK   3  TOPOLOGY FILE  2   : WATER_REP.TOP                                  
REMARK   3  TOPOLOGY FILE  3   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2Z0N COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 07-AUG-07.                  
REMARK 100 THE RCSB ID CODE IS RCSB027371.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 26-SEP-06                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 7.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SPRING-8                           
REMARK 200  BEAMLINE                       : BL44B2                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.979089, 0.979462, 0.964          
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 210                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 19533                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.950                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 7.100                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : 0.08000                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 16.8000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.95                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.02                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : 0.34100                            
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: MAD                                            
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MAD                          
REMARK 200 SOFTWARE USED: SOLVE                                                 
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 37.32                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 1.96                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 17.5% PEG3350, 0.2M AMMONIUM             
REMARK 280  SULFATE, 0.1M HEPES, VAPOR DIFFUSION, HANGING DROP,                 
REMARK 280  TEMPERATURE 293K                                                    
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z                                                 
REMARK 290       3555   -X+1/2,Y+1/2,-Z                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       27.06050            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       64.16450            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       27.06050            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       64.16450            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7550 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 25260 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -80.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    -4                                                      
REMARK 465     PRO A    -3                                                      
REMARK 465     LEU A    -2                                                      
REMARK 465     GLY A    -1                                                      
REMARK 465     SER A     0                                                      
REMARK 465     MSE A     1                                                      
REMARK 465     PRO A     2                                                      
REMARK 465     GLY A     3                                                      
REMARK 465     ILE A     4                                                      
REMARK 465     ASP A     5                                                      
REMARK 465     LYS A     6                                                      
REMARK 465     LEU A     7                                                      
REMARK 465     PRO A     8                                                      
REMARK 465     ILE A     9                                                      
REMARK 465     GLU A    10                                                      
REMARK 465     GLU A    11                                                      
REMARK 465     THR A    12                                                      
REMARK 465     ARG A    72                                                      
REMARK 465     PHE A    73                                                      
REMARK 465     PRO A    74                                                      
REMARK 465     LEU A    75                                                      
REMARK 465     GLY A    76                                                      
REMARK 465     GLY A    77                                                      
REMARK 465     ASP A    78                                                      
REMARK 465     ASP A    79                                                      
REMARK 465     VAL A   256                                                      
REMARK 465     ALA A   257                                                      
REMARK 465     SER A   258                                                      
REMARK 465     ASP A   259                                                      
REMARK 465     PRO A   260                                                      
REMARK 465     LEU A   261                                                      
REMARK 465     TYR A   262                                                      
REMARK 465     VAL A   263                                                      
REMARK 465     PRO A   264                                                      
REMARK 465     ASP A   265                                                      
REMARK 465     PRO A   266                                                      
REMARK 465     ASP A   267                                                      
REMARK 465     PRO A   268                                                      
REMARK 465     THR A   269                                                      
REMARK 465     LYS A   270                                                      
REMARK 465     PHE A   271                                                      
REMARK 465     PRO A   272                                                      
REMARK 465     VAL A   273                                                      
REMARK 465     ASN A   274                                                      
REMARK 465     ARG A   275                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    LEU A  13   CA  -  CB  -  CG  ANGL. DEV. =  14.1 DEGREES          
REMARK 500    ASP A  49   CB  -  CG  -  OD1 ANGL. DEV. =   6.8 DEGREES          
REMARK 500    LYS A 193   CD  -  CE  -  NZ  ANGL. DEV. = -14.7 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS A  70       33.42    -74.91                                   
REMARK 500    ARG A 121      -60.85   -103.16                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSO003007272.1   RELATED DB: TARGETDB                    
DBREF  2Z0N A    1   275  UNP    Q9UKG1   DP13A_HUMAN      1    275             
SEQADV 2Z0N GLY A   -4  UNP  Q9UKG1              EXPRESSION TAG                 
SEQADV 2Z0N PRO A   -3  UNP  Q9UKG1              EXPRESSION TAG                 
SEQADV 2Z0N LEU A   -2  UNP  Q9UKG1              EXPRESSION TAG                 
SEQADV 2Z0N GLY A   -1  UNP  Q9UKG1              EXPRESSION TAG                 
SEQADV 2Z0N SER A    0  UNP  Q9UKG1              EXPRESSION TAG                 
SEQRES   1 A  280  GLY PRO LEU GLY SER MSE PRO GLY ILE ASP LYS LEU PRO          
SEQRES   2 A  280  ILE GLU GLU THR LEU GLU ASP SER PRO GLN THR ARG SER          
SEQRES   3 A  280  LEU LEU GLY VAL PHE GLU GLU ASP ALA THR ALA ILE SER          
SEQRES   4 A  280  ASN TYR MSE ASN GLN LEU TYR GLN ALA MSE HIS ARG ILE          
SEQRES   5 A  280  TYR ASP ALA GLN ASN GLU LEU SER ALA ALA THR HIS LEU          
SEQRES   6 A  280  THR SER LYS LEU LEU LYS GLU TYR GLU LYS GLN ARG PHE          
SEQRES   7 A  280  PRO LEU GLY GLY ASP ASP GLU VAL MSE SER SER THR LEU          
SEQRES   8 A  280  GLN GLN PHE SER LYS VAL ILE ASP GLU LEU SER SER CYS          
SEQRES   9 A  280  HIS ALA VAL LEU SER THR GLN LEU ALA ASP ALA MSE MSE          
SEQRES  10 A  280  PHE PRO ILE THR GLN PHE LYS GLU ARG ASP LEU LYS GLU          
SEQRES  11 A  280  ILE LEU THR LEU LYS GLU VAL PHE GLN ILE ALA SER ASN          
SEQRES  12 A  280  ASP HIS ASP ALA ALA ILE ASN ARG TYR SER ARG LEU SER          
SEQRES  13 A  280  LYS LYS ARG GLU ASN ASP LYS VAL LYS TYR GLU VAL THR          
SEQRES  14 A  280  GLU ASP VAL TYR THR SER ARG LYS LYS GLN HIS GLN THR          
SEQRES  15 A  280  MSE MSE HIS TYR PHE CYS ALA LEU ASN THR LEU GLN TYR          
SEQRES  16 A  280  LYS LYS LYS ILE ALA LEU LEU GLU PRO LEU LEU GLY TYR          
SEQRES  17 A  280  MSE GLN ALA GLN ILE SER PHE PHE LYS MSE GLY SER GLU          
SEQRES  18 A  280  ASN LEU ASN GLU GLN LEU GLU GLU PHE LEU ALA ASN ILE          
SEQRES  19 A  280  GLY THR SER VAL GLN ASN VAL ARG ARG GLU MSE ASP SER          
SEQRES  20 A  280  ASP ILE GLU THR MSE GLN GLN THR ILE GLU ASP LEU GLU          
SEQRES  21 A  280  VAL ALA SER ASP PRO LEU TYR VAL PRO ASP PRO ASP PRO          
SEQRES  22 A  280  THR LYS PHE PRO VAL ASN ARG                                  
MODRES 2Z0N MSE A   37  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A   44  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A   82  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A  111  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A  112  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A  178  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A  179  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A  204  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A  213  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A  240  MET  SELENOMETHIONINE                                   
MODRES 2Z0N MSE A  247  MET  SELENOMETHIONINE                                   
HET    MSE  A  37       8                                                       
HET    MSE  A  44       8                                                       
HET    MSE  A  82       8                                                       
HET    MSE  A 111       8                                                       
HET    MSE  A 112       8                                                       
HET    MSE  A 178       8                                                       
HET    MSE  A 179       8                                                       
HET    MSE  A 204       8                                                       
HET    MSE  A 213       8                                                       
HET    MSE  A 240       8                                                       
HET    MSE  A 247       8                                                       
HETNAM     MSE SELENOMETHIONINE                                                 
FORMUL   1  MSE    11(C5 H11 N O2 SE)                                           
FORMUL   2  HOH   *96(H2 O)                                                     
HELIX    1   1 ASP A   15  GLU A   67  1                                  53    
HELIX    2   2 TYR A   68  GLN A   71  5                                   4    
HELIX    3   3 GLU A   80  MSE A  111  1                                  32    
HELIX    4   4 MSE A  111  ARG A  121  1                                  11    
HELIX    5   5 ARG A  121  LYS A  152  1                                  32    
HELIX    6   6 ASN A  156  LEU A  218  1                                  63    
HELIX    7   7 ASN A  219  GLU A  255  1                                  37    
LINK         C   TYR A  36                 N   MSE A  37     1555   1555  1.33  
LINK         C   MSE A  37                 N   ASN A  38     1555   1555  1.33  
LINK         C   ALA A  43                 N   MSE A  44     1555   1555  1.32  
LINK         C   MSE A  44                 N   HIS A  45     1555   1555  1.33  
LINK         C   VAL A  81                 N   MSE A  82     1555   1555  1.32  
LINK         C   MSE A  82                 N   SER A  83     1555   1555  1.34  
LINK         C   ALA A 110                 N   MSE A 111     1555   1555  1.33  
LINK         C   MSE A 111                 N   MSE A 112     1555   1555  1.35  
LINK         C   MSE A 112                 N   PHE A 113     1555   1555  1.32  
LINK         C   THR A 177                 N   MSE A 178     1555   1555  1.34  
LINK         C   MSE A 178                 N   MSE A 179     1555   1555  1.34  
LINK         C   MSE A 179                 N   HIS A 180     1555   1555  1.34  
LINK         C   TYR A 203                 N   MSE A 204     1555   1555  1.34  
LINK         C   MSE A 204                 N   GLN A 205     1555   1555  1.34  
LINK         C   LYS A 212                 N   MSE A 213     1555   1555  1.32  
LINK         C   MSE A 213                 N   GLY A 214     1555   1555  1.33  
LINK         C   GLU A 239                 N   MSE A 240     1555   1555  1.35  
LINK         C   MSE A 240                 N   ASP A 241     1555   1555  1.34  
LINK         C   THR A 246                 N   MSE A 247     1555   1555  1.33  
LINK         C   MSE A 247                 N   GLN A 248     1555   1555  1.34  
CRYST1   54.121  128.329   36.964  90.00  90.00  90.00 P 21 21 2     4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.018477  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.007792  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.027053        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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