GenomeNet

Database: PDB
Entry: 3TV0
LinkDB: 3TV0
Original site: 3TV0 
HEADER    STRUCTURAL PROTEIN                      19-SEP-11   3TV0              
TITLE     STRUCTURE OF DYNACTIN P27 SUBUNIT                                     
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DYNACTIN SUBUNIT 6;                                        
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: DYNACTIN SUBUNIT P27, PROTEIN WS-3;                         
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: DCTN6, WS3;                                                    
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    LEFT-HANDED BETA-HELIX, P25, P62, ARP11, CYTOSOL, STRUCTURAL PROTEIN  
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Z.S.DEREWENDA,U.DEREWENDA,A.KOWALSKA                                  
REVDAT   1   03-OCT-12 3TV0    0                                                
JRNL        AUTH   Z.S.DEREWENDA,U.DEREWENDA,A.KOWALSKA,M.ZHENG                 
JRNL        TITL   DYNACTIN TARGETS POLO-LIKE KINASE 1 TO KINETOCHORES          
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.15 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.6.4_486)                    
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VICENT CHEN,IAN             
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-                     
REMARK   3               : KUNSTLEVE,LI-WEI HUNG,ROBERT IMMORMINO,              
REMARK   3               : TOM IOERGER,AIRLIE MCCOY,ERIK MCKEE,NIGEL            
REMARK   3               : MORIARTY,REETAL PAI,RANDY READ,JANE                  
REMARK   3               : RICHARDSON,DAVID RICHARDSON,TOD ROMO,JIM             
REMARK   3               : SACCHETTINI,NICHOLAS SAUTER,JACOB SMITH,             
REMARK   3               : LAURENT STORONI,TOM TERWILLIGER,PETER                
REMARK   3               : ZWART                                                
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.15                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 43.45                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.360                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 81.1                           
REMARK   3   NUMBER OF REFLECTIONS             : 16255                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.216                           
REMARK   3   R VALUE            (WORKING SET) : 0.214                           
REMARK   3   FREE R VALUE                     : 0.258                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.030                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 817                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 43.4595 -  3.9045    0.98     3199   166  0.2143 0.2375        
REMARK   3     2  3.9045 -  3.0994    0.99     3127   168  0.1985 0.2493        
REMARK   3     3  3.0994 -  2.7077    0.96     3018   168  0.2224 0.2907        
REMARK   3     4  2.7077 -  2.4601    0.84     2646   133  0.2406 0.3100        
REMARK   3     5  2.4601 -  2.2838    0.63     1991   105  0.2234 0.2502        
REMARK   3     6  2.2838 -  2.1492    0.46     1457    77  0.2155 0.2905        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.20                                          
REMARK   3   SHRINKAGE RADIUS   : 0.95                                          
REMARK   3   K_SOL              : 0.39                                          
REMARK   3   B_SOL              : 46.95                                         
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.330            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 30.360           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 0.00000                                              
REMARK   3    B22 (A**2) : 0.00000                                              
REMARK   3    B33 (A**2) : 0.00000                                              
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.008           2178                                  
REMARK   3   ANGLE     :  1.144           2961                                  
REMARK   3   CHIRALITY :  0.074            357                                  
REMARK   3   PLANARITY :  0.005            389                                  
REMARK   3   DIHEDRAL  : 12.973            822                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 3TV0 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 27-SEP-11.                  
REMARK 100 THE RCSB ID CODE IS RCSB067953.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 27-JUN-10                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 3.6                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 22-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1                                  
REMARK 200  MONOCHROMATOR                  : SI 111                             
REMARK 200  OPTICS                         : SI(111)                            
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 300 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 16255                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.149                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 43.500                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 5.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 81.1                               
REMARK 200  DATA REDUNDANCY                : NULL                               
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : NULL                               
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.15                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.19                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 38.4                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: BALBES                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 43.79                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.19                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 19% PEG 2000, 0.15 M POTASSIUM           
REMARK 280  PHOSPHATE, PH 3.6, VAPOR DIFFUSION, TEMPERATURE 295K                
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       42.48000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       28.57000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       42.48000            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       28.57000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2530 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 12170 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -11.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B                                     
REMARK 350   BIOMT1   2  1.000000  0.000000  0.000000      -42.48000            
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000       28.57000            
REMARK 350   BIOMT3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 6820 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 22590 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -34.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000      -25.44466            
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000       76.04607            
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B                                     
REMARK 350   BIOMT1   3  1.000000  0.000000  0.000000      -42.48000            
REMARK 350   BIOMT2   3  0.000000  1.000000  0.000000       28.57000            
REMARK 350   BIOMT3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   4 -1.000000  0.000000  0.000000       17.03534            
REMARK 350   BIOMT2   4  0.000000  1.000000  0.000000       28.57000            
REMARK 350   BIOMT3   4  0.000000  0.000000 -1.000000       76.04607            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    -3                                                      
REMARK 465     SER A    -2                                                      
REMARK 465     SER A    -1                                                      
REMARK 465     SER A     0                                                      
REMARK 465     MET A     1                                                      
REMARK 465     ALA A     2                                                      
REMARK 465     GLU A     3                                                      
REMARK 465     LYS A     4                                                      
REMARK 465     THR A     5                                                      
REMARK 465     GLN A     6                                                      
REMARK 465     LYS A     7                                                      
REMARK 465     THR A    71                                                      
REMARK 465     PRO A    72                                                      
REMARK 465     ASP A    73                                                      
REMARK 465     THR A    74                                                      
REMARK 465     GLU A    75                                                      
REMARK 465     ASP A    76                                                      
REMARK 465     PRO A    77                                                      
REMARK 465     GLU A    78                                                      
REMARK 465     PRO A    79                                                      
REMARK 465     GLN A   160                                                      
REMARK 465     THR A   161                                                      
REMARK 465     LEU A   162                                                      
REMARK 465     GLN A   163                                                      
REMARK 465     LEU A   164                                                      
REMARK 465     ASP A   165                                                      
REMARK 465     PHE A   166                                                      
REMARK 465     LEU A   167                                                      
REMARK 465     MET A   168                                                      
REMARK 465     LYS A   169                                                      
REMARK 465     ILE A   170                                                      
REMARK 465     LEU A   171                                                      
REMARK 465     PRO A   172                                                      
REMARK 465     ASN A   173                                                      
REMARK 465     TYR A   174                                                      
REMARK 465     HIS A   175                                                      
REMARK 465     HIS A   176                                                      
REMARK 465     LEU A   177                                                      
REMARK 465     LYS A   178                                                      
REMARK 465     LYS A   179                                                      
REMARK 465     THR A   180                                                      
REMARK 465     MET A   181                                                      
REMARK 465     LYS A   182                                                      
REMARK 465     GLY A   183                                                      
REMARK 465     SER A   184                                                      
REMARK 465     SER A   185                                                      
REMARK 465     THR A   186                                                      
REMARK 465     PRO A   187                                                      
REMARK 465     VAL A   188                                                      
REMARK 465     LYS A   189                                                      
REMARK 465     ASN A   190                                                      
REMARK 465     GLY B    -3                                                      
REMARK 465     SER B    -2                                                      
REMARK 465     SER B    -1                                                      
REMARK 465     SER B     0                                                      
REMARK 465     MET B     1                                                      
REMARK 465     ALA B     2                                                      
REMARK 465     GLU B     3                                                      
REMARK 465     LYS B     4                                                      
REMARK 465     THR B     5                                                      
REMARK 465     GLN B     6                                                      
REMARK 465     LYS B     7                                                      
REMARK 465     SER B     8                                                      
REMARK 465     THR B    71                                                      
REMARK 465     PRO B    72                                                      
REMARK 465     ASP B    73                                                      
REMARK 465     THR B    74                                                      
REMARK 465     GLU B    75                                                      
REMARK 465     ASP B    76                                                      
REMARK 465     PRO B    77                                                      
REMARK 465     GLU B    78                                                      
REMARK 465     GLN B   158                                                      
REMARK 465     PRO B   159                                                      
REMARK 465     GLN B   160                                                      
REMARK 465     THR B   161                                                      
REMARK 465     LEU B   162                                                      
REMARK 465     GLN B   163                                                      
REMARK 465     LEU B   164                                                      
REMARK 465     ASP B   165                                                      
REMARK 465     PHE B   166                                                      
REMARK 465     LEU B   167                                                      
REMARK 465     MET B   168                                                      
REMARK 465     LYS B   169                                                      
REMARK 465     ILE B   170                                                      
REMARK 465     LEU B   171                                                      
REMARK 465     PRO B   172                                                      
REMARK 465     ASN B   173                                                      
REMARK 465     TYR B   174                                                      
REMARK 465     HIS B   175                                                      
REMARK 465     HIS B   176                                                      
REMARK 465     LEU B   177                                                      
REMARK 465     LYS B   178                                                      
REMARK 465     LYS B   179                                                      
REMARK 465     THR B   180                                                      
REMARK 465     MET B   181                                                      
REMARK 465     LYS B   182                                                      
REMARK 465     GLY B   183                                                      
REMARK 465     SER B   184                                                      
REMARK 465     SER B   185                                                      
REMARK 465     THR B   186                                                      
REMARK 465     PRO B   187                                                      
REMARK 465     VAL B   188                                                      
REMARK 465     LYS B   189                                                      
REMARK 465     ASN B   190                                                      
REMARK 480                                                                      
REMARK 480 ZERO OCCUPANCY ATOM                                                  
REMARK 480 THE FOLLOWING RESIDUES HAVE ATOMS MODELED WITH ZERO                  
REMARK 480 OCCUPANCY. THE LOCATION AND PROPERTIES OF THESE ATOMS                
REMARK 480 MAY NOT BE RELIABLE. (M=MODEL NUMBER; RES=RESIDUE NAME;              
REMARK 480 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):         
REMARK 480   M RES C SSEQI ATOMS                                                
REMARK 480     ILE B   48   CA   CB   CG1  CG2  CD1                             
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASP A  68       57.73    -97.60                                   
REMARK 500    ASN A  69      160.47    172.65                                   
REMARK 500    SER A 109      124.52    -38.55                                   
REMARK 500    THR A 120     -110.10    -89.08                                   
REMARK 500    CYS A 128        1.49     59.59                                   
REMARK 500    ASN A 132       68.30   -106.48                                   
REMARK 500    ARG B  32       -2.06     74.21                                   
REMARK 500    ASP B  68       43.99    -82.10                                   
REMARK 500    THR B 120     -107.68    -85.36                                   
REMARK 500    CYS B 128        9.46     59.17                                   
REMARK 500    GLU B 135      162.47    176.29                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  3TV0 A    1   190  UNP    O00399   DCTN6_HUMAN      1    190             
DBREF  3TV0 B    1   190  UNP    O00399   DCTN6_HUMAN      1    190             
SEQADV 3TV0 GLY A   -3  UNP  O00399              EXPRESSION TAG                 
SEQADV 3TV0 SER A   -2  UNP  O00399              EXPRESSION TAG                 
SEQADV 3TV0 SER A   -1  UNP  O00399              EXPRESSION TAG                 
SEQADV 3TV0 SER A    0  UNP  O00399              EXPRESSION TAG                 
SEQADV 3TV0 GLY B   -3  UNP  O00399              EXPRESSION TAG                 
SEQADV 3TV0 SER B   -2  UNP  O00399              EXPRESSION TAG                 
SEQADV 3TV0 SER B   -1  UNP  O00399              EXPRESSION TAG                 
SEQADV 3TV0 SER B    0  UNP  O00399              EXPRESSION TAG                 
SEQRES   1 A  194  GLY SER SER SER MET ALA GLU LYS THR GLN LYS SER VAL          
SEQRES   2 A  194  LYS ILE ALA PRO GLY ALA VAL VAL CYS VAL GLU SER GLU          
SEQRES   3 A  194  ILE ARG GLY ASP VAL THR ILE GLY PRO ARG THR VAL ILE          
SEQRES   4 A  194  HIS PRO LYS ALA ARG ILE ILE ALA GLU ALA GLY PRO ILE          
SEQRES   5 A  194  VAL ILE GLY GLU GLY ASN LEU ILE GLU GLU GLN ALA LEU          
SEQRES   6 A  194  ILE ILE ASN ALA TYR PRO ASP ASN ILE THR PRO ASP THR          
SEQRES   7 A  194  GLU ASP PRO GLU PRO LYS PRO MET ILE ILE GLY THR ASN          
SEQRES   8 A  194  ASN VAL PHE GLU VAL GLY CYS TYR SER GLN ALA MET LYS          
SEQRES   9 A  194  MET GLY ASP ASN ASN VAL ILE GLU SER LYS ALA TYR VAL          
SEQRES  10 A  194  GLY ARG ASN VAL ILE LEU THR SER GLY CYS ILE ILE GLY          
SEQRES  11 A  194  ALA CYS CYS ASN LEU ASN THR PHE GLU VAL ILE PRO GLU          
SEQRES  12 A  194  ASN THR VAL ILE TYR GLY ALA ASP CYS LEU ARG ARG VAL          
SEQRES  13 A  194  GLN THR GLU ARG PRO GLN PRO GLN THR LEU GLN LEU ASP          
SEQRES  14 A  194  PHE LEU MET LYS ILE LEU PRO ASN TYR HIS HIS LEU LYS          
SEQRES  15 A  194  LYS THR MET LYS GLY SER SER THR PRO VAL LYS ASN              
SEQRES   1 B  194  GLY SER SER SER MET ALA GLU LYS THR GLN LYS SER VAL          
SEQRES   2 B  194  LYS ILE ALA PRO GLY ALA VAL VAL CYS VAL GLU SER GLU          
SEQRES   3 B  194  ILE ARG GLY ASP VAL THR ILE GLY PRO ARG THR VAL ILE          
SEQRES   4 B  194  HIS PRO LYS ALA ARG ILE ILE ALA GLU ALA GLY PRO ILE          
SEQRES   5 B  194  VAL ILE GLY GLU GLY ASN LEU ILE GLU GLU GLN ALA LEU          
SEQRES   6 B  194  ILE ILE ASN ALA TYR PRO ASP ASN ILE THR PRO ASP THR          
SEQRES   7 B  194  GLU ASP PRO GLU PRO LYS PRO MET ILE ILE GLY THR ASN          
SEQRES   8 B  194  ASN VAL PHE GLU VAL GLY CYS TYR SER GLN ALA MET LYS          
SEQRES   9 B  194  MET GLY ASP ASN ASN VAL ILE GLU SER LYS ALA TYR VAL          
SEQRES  10 B  194  GLY ARG ASN VAL ILE LEU THR SER GLY CYS ILE ILE GLY          
SEQRES  11 B  194  ALA CYS CYS ASN LEU ASN THR PHE GLU VAL ILE PRO GLU          
SEQRES  12 B  194  ASN THR VAL ILE TYR GLY ALA ASP CYS LEU ARG ARG VAL          
SEQRES  13 B  194  GLN THR GLU ARG PRO GLN PRO GLN THR LEU GLN LEU ASP          
SEQRES  14 B  194  PHE LEU MET LYS ILE LEU PRO ASN TYR HIS HIS LEU LYS          
SEQRES  15 B  194  LYS THR MET LYS GLY SER SER THR PRO VAL LYS ASN              
FORMUL   3  HOH   *74(H2 O)                                                     
SHEET    1   A 7 VAL A   9  ILE A  11  0                                        
SHEET    2   A 7 VAL A  27  ILE A  29  1  O  VAL A  27   N  LYS A  10           
SHEET    3   A 7 ILE A  48  ILE A  50  1  O  ILE A  50   N  THR A  28           
SHEET    4   A 7 MET A  82  ILE A  84  1  O  MET A  82   N  VAL A  49           
SHEET    5   A 7 LYS A 100  MET A 101  1  O  MET A 101   N  ILE A  83           
SHEET    6   A 7 VAL A 117  LEU A 119  1  O  LEU A 119   N  LYS A 100           
SHEET    7   A 7 GLU A 135  ILE A 137  1  O  ILE A 137   N  ILE A 118           
SHEET    1   B 8 VAL A  16  VAL A  17  0                                        
SHEET    2   B 8 VAL A  34  ILE A  35  1  O  ILE A  35   N  VAL A  16           
SHEET    3   B 8 LEU A  55  ILE A  56  1  O  ILE A  56   N  VAL A  34           
SHEET    4   B 8 VAL A  89  PHE A  90  1  O  PHE A  90   N  LEU A  55           
SHEET    5   B 8 VAL A 106  ILE A 107  1  O  ILE A 107   N  VAL A  89           
SHEET    6   B 8 ILE A 124  ILE A 125  1  O  ILE A 125   N  VAL A 106           
SHEET    7   B 8 THR A 141  TYR A 144  1  O  THR A 141   N  ILE A 124           
SHEET    8   B 8 ARG A 150  GLN A 153 -1  O  VAL A 152   N  VAL A 142           
SHEET    1   C 6 GLU A  22  ARG A  24  0                                        
SHEET    2   C 6 ARG A  40  ALA A  43  1  O  ILE A  41   N  GLU A  22           
SHEET    3   C 6 LEU A  61  ASN A  64  1  O  ILE A  62   N  ARG A  40           
SHEET    4   C 6 TYR A  95  SER A  96  1  O  SER A  96   N  LEU A  61           
SHEET    5   C 6 TYR A 112  VAL A 113  1  O  VAL A 113   N  TYR A  95           
SHEET    6   C 6 ASN A 130  LEU A 131  1  O  LEU A 131   N  TYR A 112           
SHEET    1   D 7 LYS B  10  ILE B  11  0                                        
SHEET    2   D 7 GLU B  22  ILE B  29  1  O  ILE B  29   N  LYS B  10           
SHEET    3   D 7 ARG B  40  ILE B  50  1  O  ILE B  41   N  GLU B  22           
SHEET    4   D 7 LEU B  61  ASN B  64  1  O  ILE B  62   N  ARG B  40           
SHEET    5   D 7 TYR B  95  SER B  96  1  O  SER B  96   N  LEU B  61           
SHEET    6   D 7 TYR B 112  VAL B 113  1  O  VAL B 113   N  TYR B  95           
SHEET    7   D 7 ASN B 130  LEU B 131  1  O  LEU B 131   N  TYR B 112           
SHEET    1   E 7 LYS B  10  ILE B  11  0                                        
SHEET    2   E 7 GLU B  22  ILE B  29  1  O  ILE B  29   N  LYS B  10           
SHEET    3   E 7 ARG B  40  ILE B  50  1  O  ILE B  41   N  GLU B  22           
SHEET    4   E 7 MET B  82  ILE B  84  1  O  MET B  82   N  PRO B  47           
SHEET    5   E 7 LYS B 100  MET B 101  1  O  MET B 101   N  ILE B  83           
SHEET    6   E 7 VAL B 117  LEU B 119  1  O  LEU B 119   N  LYS B 100           
SHEET    7   E 7 GLU B 135  ILE B 137  1  O  GLU B 135   N  ILE B 118           
SHEET    1   F 8 VAL B  16  VAL B  17  0                                        
SHEET    2   F 8 VAL B  34  ILE B  35  1  O  ILE B  35   N  VAL B  16           
SHEET    3   F 8 LEU B  55  ILE B  56  1  O  ILE B  56   N  VAL B  34           
SHEET    4   F 8 VAL B  89  PHE B  90  1  O  PHE B  90   N  LEU B  55           
SHEET    5   F 8 VAL B 106  ILE B 107  1  O  ILE B 107   N  VAL B  89           
SHEET    6   F 8 ILE B 124  ILE B 125  1  O  ILE B 125   N  VAL B 106           
SHEET    7   F 8 THR B 141  TYR B 144  1  O  ILE B 143   N  ILE B 124           
SHEET    8   F 8 ARG B 150  GLN B 153 -1  O  VAL B 152   N  VAL B 142           
SSBOND   1 CYS A  129    CYS A  148                          1555   1555  2.05  
SSBOND   2 CYS B  129    CYS B  148                          1555   1555  2.05  
CRYST1   84.960   57.140   80.190  90.00 108.50  90.00 C 1 2 1       8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.011770  0.000000  0.003938        0.00000                         
SCALE2      0.000000  0.017501  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.013150        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system