GenomeNet

Database: PDB
Entry: 4G69
LinkDB: 4G69
Original site: 4G69 
HEADER    MEMBRANE PROTEIN/SIGNALING PROTEIN      18-JUL-12   4G69              
TITLE     STRUCTURE OF THE HUMAN DISCS LARGE 1 PDZ2 - ADENOMATOUS POLYPOSIS COLI
TITLE    2 CYTOSKELETAL POLARITY COMPLEX                                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DISKS LARGE HOMOLOG 1;                                     
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: PDZ2, UNP RESIDUES 310-407;                                
COMPND   5 SYNONYM: SYNAPSE-ASSOCIATED PROTEIN 97, SAP-97, SAP97, HDLG;         
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: ADENOMATOUS POLYPOSIS COLI PROTEIN;                        
COMPND   9 CHAIN: B;                                                            
COMPND  10 FRAGMENT: APC C-TERMINAL PEPTIDE, UNP RESIDUES 2833-2843;            
COMPND  11 SYNONYM: PROTEIN APC, DELETED IN POLYPOSIS 2.5;                      
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: DLG1;                                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3) PLYSS;                           
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PGEX-2T;                                  
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 SYNTHETIC: YES;                                                      
SOURCE  13 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  14 ORGANISM_COMMON: HUMAN;                                              
SOURCE  15 ORGANISM_TAXID: 9606;                                                
SOURCE  16 OTHER_DETAILS: ADENOMATOUS POLYPOSIS COLI (APC) C-TERMINAL 11        
SOURCE  17 RESIDUES SYNTHESIZED WITH N-TERMINAL TETRAMETHYL RHODAMINE TAG       
KEYWDS    PDZ, MAGUK, POLARITY, ADENOMATOUS POLYPOSIS COLI,                     
KEYWDS   2 TETRAMETHYLRHODAMINE, ADHERENS JUNCTION, MEMBRANE PROTEIN-SIGNALING  
KEYWDS   3 PROTEIN COMPLEX                                                      
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    K.C.SLEP                                                              
REVDAT   2   15-NOV-17 4G69    1       REMARK                                   
REVDAT   1   12-DEC-12 4G69    0                                                
JRNL        AUTH   K.C.SLEP                                                     
JRNL        TITL   STRUCTURE OF THE HUMAN DISCS LARGE 1 PDZ2- ADENOMATOUS       
JRNL        TITL 2 POLYPOSIS COLI CYTOSKELETAL POLARITY COMPLEX: INSIGHT INTO   
JRNL        TITL 3 PEPTIDE ENGAGEMENT AND PDZ CLUSTERING.                       
JRNL        REF    PLOS ONE                      V.   7 50097 2012              
JRNL        REFN                   ESSN 1932-6203                               
JRNL        PMID   23185543                                                     
JRNL        DOI    10.1371/JOURNAL.PONE.0050097                                 
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.00 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.6.1_357)                    
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.00                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 29.38                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 0.100                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 95.1                           
REMARK   3   NUMBER OF REFLECTIONS             : 6846                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.172                           
REMARK   3   R VALUE            (WORKING SET) : 0.171                           
REMARK   3   FREE R VALUE                     : 0.183                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 10.050                          
REMARK   3   FREE R VALUE TEST SET COUNT      : 688                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 29.3823 -  3.4146    0.99     1351   151  0.1676 0.1678        
REMARK   3     2  3.4146 -  2.7108    0.98     1277   143  0.1763 0.1821        
REMARK   3     3  2.7108 -  2.3683    0.97     1255   139  0.1782 0.1913        
REMARK   3     4  2.3683 -  2.1519    0.96     1208   139  0.1651 0.2035        
REMARK   3     5  2.1519 -  1.9977    0.85     1067   116  0.1640 0.1872        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : 0.37                                          
REMARK   3   B_SOL              : 32.34                                         
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.170            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 15.050           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -2.88850                                             
REMARK   3    B22 (A**2) : 0.33000                                              
REMARK   3    B33 (A**2) : 2.55860                                              
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.007            779                                  
REMARK   3   ANGLE     :  0.980           1051                                  
REMARK   3   CHIRALITY :  0.063            127                                  
REMARK   3   PLANARITY :  0.004            132                                  
REMARK   3   DIHEDRAL  : 12.794            288                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 1                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: ALL                                                    
REMARK   3    ORIGIN FOR THE GROUP (A):  -4.1675  -4.9337  14.8774              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0144 T22:   0.0142                                     
REMARK   3      T33:   0.0138 T12:  -0.0003                                     
REMARK   3      T13:   0.0033 T23:  -0.0293                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.4398 L22:   0.3595                                     
REMARK   3      L33:   0.2922 L12:   0.0187                                     
REMARK   3      L13:   0.2044 L23:  -0.2575                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0080 S12:   0.0054 S13:  -0.0127                       
REMARK   3      S21:  -0.0022 S22:  -0.0015 S23:  -0.0104                       
REMARK   3      S31:   0.0030 S32:  -0.0302 S33:   0.0062                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4G69 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 27-JUL-12.                  
REMARK 100 THE DEPOSITION ID IS D_1000073790.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 08-MAR-07                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.25                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ALS                                
REMARK 200  BEAMLINE                       : 8.3.1                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.127                              
REMARK 200  MONOCHROMATOR                  : COLLECTED AT 1.127 A               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 210                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 6846                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.000                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 70.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 97.8                               
REMARK 200  DATA REDUNDANCY                : 4.000                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : 0.07000                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 20.7000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.00                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.07                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 82.9                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.00                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : 0.22000                            
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 4.800                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHENIX                                                
REMARK 200 STARTING MODEL: PDB ENTRY 2FE5                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 41.65                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.11                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: PROTEIN SOLUTION: 1.4 MM DLG1 PDZ2,      
REMARK 280  1.7 MM APC, 100 MM NACL, 25 MM TRIS PH 7.0, 0.1% B-ME WELL: 200     
REMARK 280  MM SODIUM ACETATE, 100 MM SODIUM CACODYLATE PH 6.25, 28% PEG        
REMARK 280  8000 DROP = 1:1 PROTEIN:WELL SOLUTION , VAPOR DIFFUSION, HANGING    
REMARK 280  DROP, TEMPERATURE 300K                                              
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       13.55450            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       35.87800            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       25.59200            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       35.87800            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       13.55450            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       25.59200            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 740 ANGSTROM**2                           
REMARK 350 SURFACE AREA OF THE COMPLEX: 6190 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -4.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A   308                                                      
REMARK 465     SER A   309                                                      
REMARK 465     SER A   407                                                      
REMARK 465     ARG B  2833                                                      
REMARK 465     HIS B  2834                                                      
REMARK 465     SER B  2835                                                      
REMARK 465     GLY B  2836                                                      
REMARK 465     SER B  2837                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ARG A 310    CG   CD   NE   CZ   NH1  NH2                        
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    HOH A   553     O    HOH A   602              2.08            
REMARK 500   O    HOH A   534     O    HOH A   560              2.10            
REMARK 500   O    HOH A   558     O    HOH A   579              2.12            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN A 339       39.65   -153.33                                   
REMARK 500    ASN A 375     -116.96     52.29                                   
REMARK 500    ASN A 393       54.81    -90.89                                   
REMARK 500    SER A 395     -155.71    -92.19                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  4G69 A  310   407  UNP    Q12959   DLG1_HUMAN     310    407             
DBREF  4G69 B 2833  2843  UNP    P25054   APC_HUMAN     2833   2843             
SEQADV 4G69 GLY A  308  UNP  Q12959              EXPRESSION TAG                 
SEQADV 4G69 SER A  309  UNP  Q12959              EXPRESSION TAG                 
SEQRES   1 A  100  GLY SER ARG LYS PRO VAL SER GLU LYS ILE MET GLU ILE          
SEQRES   2 A  100  LYS LEU ILE LYS GLY PRO LYS GLY LEU GLY PHE SER ILE          
SEQRES   3 A  100  ALA GLY GLY VAL GLY ASN GLN HIS ILE PRO GLY ASP ASN          
SEQRES   4 A  100  SER ILE TYR VAL THR LYS ILE ILE GLU GLY GLY ALA ALA          
SEQRES   5 A  100  HIS LYS ASP GLY LYS LEU GLN ILE GLY ASP LYS LEU LEU          
SEQRES   6 A  100  ALA VAL ASN ASN VAL CYS LEU GLU GLU VAL THR HIS GLU          
SEQRES   7 A  100  GLU ALA VAL THR ALA LEU LYS ASN THR SER ASP PHE VAL          
SEQRES   8 A  100  TYR LEU LYS VAL ALA LYS PRO THR SER                          
SEQRES   1 B   11  ARG HIS SER GLY SER TYR LEU VAL THR SER VAL                  
FORMUL   3  HOH   *106(H2 O)                                                    
HELIX    1   1 GLY A  357  GLY A  363  1                                   7    
HELIX    2   2 THR A  383  ASN A  393  1                                  11    
SHEET    1   A 4 GLU A 315  ILE A 323  0                                        
SHEET    2   A 4 PHE A 397  PRO A 405 -1  O  LEU A 400   N  ILE A 320           
SHEET    3   A 4 LYS A 370  VAL A 374 -1  N  LEU A 372   O  LYS A 401           
SHEET    4   A 4 VAL A 377  CYS A 378 -1  O  VAL A 377   N  VAL A 374           
SHEET    1   B 6 GLU A 315  ILE A 323  0                                        
SHEET    2   B 6 PHE A 397  PRO A 405 -1  O  LEU A 400   N  ILE A 320           
SHEET    3   B 6 LYS A 370  VAL A 374 -1  N  LEU A 372   O  LYS A 401           
SHEET    4   B 6 ILE A 348  ILE A 353 -1  N  ILE A 348   O  LEU A 371           
SHEET    5   B 6 PHE A 331  GLY A 335 -1  N  ALA A 334   O  TYR A 349           
SHEET    6   B 6 THR B2841  SER B2842 -1  O  THR B2841   N  ILE A 333           
CRYST1   27.109   51.184   71.756  90.00  90.00  90.00 P 21 21 21    4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.036888  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.019537  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.013936        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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