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Database: PDB
Entry: 4P3E
LinkDB: 4P3E
Original site: 4P3E 
HEADER    RNA BINDING PROTEIN/RNA                 25-FEB-14   4P3E              
TITLE     STRUCTURE OF THE HUMAN SRP S DOMAIN                                   
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: SRP RNA (124-MER);                                         
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: GB RESIDUES 234-358;                                       
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MUTATION: YES;                                                       
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN;                
COMPND   9 CHAIN: B;                                                            
COMPND  10 FRAGMENT: UNP RESIDUES 1-120;                                        
COMPND  11 SYNONYM: SRP19;                                                      
COMPND  12 ENGINEERED: YES;                                                     
COMPND  13 MOL_ID: 3;                                                           
COMPND  14 MOLECULE: SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68;                 
COMPND  15 CHAIN: C;                                                            
COMPND  16 FRAGMENT: UNP RESIDUES 47-254;                                       
COMPND  17 SYNONYM: SRP68, SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN;          
COMPND  18 ENGINEERED: YES;                                                     
COMPND  19 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   4 ORGANISM_COMMON: HUMAN;                                              
SOURCE   5 ORGANISM_TAXID: 9606;                                                
SOURCE   6 MOL_ID: 2;                                                           
SOURCE   7 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   8 ORGANISM_COMMON: HUMAN;                                              
SOURCE   9 ORGANISM_TAXID: 9606;                                                
SOURCE  10 GENE: SRP19;                                                         
SOURCE  11 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  12 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  13 MOL_ID: 3;                                                           
SOURCE  14 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  15 ORGANISM_COMMON: HUMAN;                                              
SOURCE  16 ORGANISM_TAXID: 9606;                                                
SOURCE  17 GENE: SRP68;                                                         
SOURCE  18 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  19 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    SRP, SRP RNA, SRP19, SRP68, RIBONUCLEOPROTEIN PARTICLE (RNP),         
KEYWDS   2 ARGININE-RICH MOTIF (ARM), RNA BINDING PROTEIN-RNA COMPLEX           
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.T.GROTWINKEL,K.WILD,I.SINNING                                       
REVDAT   5   08-JAN-20 4P3E    1       REMARK                                   
REVDAT   4   27-SEP-17 4P3E    1       COMPND SOURCE KEYWDS JRNL                
REVDAT   4 2                   1       REMARK CRYST1                            
REVDAT   3   09-JUL-14 4P3E    1       KEYWDS                                   
REVDAT   2   23-APR-14 4P3E    1       REMARK                                   
REVDAT   1   16-APR-14 4P3E    0                                                
JRNL        AUTH   J.T.GROTWINKEL,K.WILD,B.SEGNITZ,I.SINNING                    
JRNL        TITL   SRP RNA REMODELING BY SRP68 EXPLAINS ITS ROLE IN PROTEIN     
JRNL        TITL 2 TRANSLOCATION.                                               
JRNL        REF    SCIENCE                       V. 344   101 2014              
JRNL        REFN                   ESSN 1095-9203                               
JRNL        PMID   24700861                                                     
JRNL        DOI    10.1126/SCIENCE.1249094                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.50 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.8.1_1168)                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.50                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 44.09                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.6                           
REMARK   3   NUMBER OF REFLECTIONS             : 11602                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.246                           
REMARK   3   R VALUE            (WORKING SET) : 0.244                           
REMARK   3   FREE R VALUE                     : 0.285                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.740                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 550                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 44.0920 -  5.5533    1.00     2839   137  0.2069 0.2183        
REMARK   3     2  5.5533 -  4.4091    0.99     2761   150  0.2474 0.3195        
REMARK   3     3  4.4091 -  3.8522    0.99     2750   135  0.2710 0.3458        
REMARK   3     4  3.8522 -  3.5001    0.97     2702   128  0.3349 0.3989        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.640            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 37.420           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 92.36                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 55.74                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.005           5511                                  
REMARK   3   ANGLE     :  1.024           8085                                  
REMARK   3   CHIRALITY :  0.059            995                                  
REMARK   3   PLANARITY :  0.004            542                                  
REMARK   3   DIHEDRAL  : 18.143           2490                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4P3E COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 11-MAR-14.                  
REMARK 100 THE DEPOSITION ID IS D_1000200370.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 08-MAY-13                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.2                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ESRF                               
REMARK 200  BEAMLINE                       : ID29                               
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : NULL                               
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9763                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M                 
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : NULL                               
REMARK 200  DATA SCALING SOFTWARE          : NULL                               
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 11703                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.500                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 44.090                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.5                               
REMARK 200  DATA REDUNDANCY                : 3.400                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 9.0000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.50                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.85                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.5                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 5.70                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.36000                            
REMARK 200  R SYM FOR SHELL            (I) : 0.02100                            
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 4P3F AND 3KTV                                        
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 57.09                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.87                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: AMMONIUM SULFATE, SODIUM MALONATE, PH    
REMARK 280  5.2, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K                
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       62.82450            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       35.26500            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       62.82450            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       35.26500            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TRIMERIC                   
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 4800 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 32440 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -62.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465       A A   238                                                      
REMARK 465     MET B     1                                                      
REMARK 465     ALA B     2                                                      
REMARK 465     CYS B     3                                                      
REMARK 465     ALA B     4                                                      
REMARK 465     ALA B     5                                                      
REMARK 465     ALA B     6                                                      
REMARK 465     ARG B     7                                                      
REMARK 465     SER B     8                                                      
REMARK 465     PRO B     9                                                      
REMARK 465     ALA B    10                                                      
REMARK 465     ASP B    11                                                      
REMARK 465     GLN B    12                                                      
REMARK 465     ARG B   118                                                      
REMARK 465     THR B   119                                                      
REMARK 465     GLN B   120                                                      
REMARK 465     LEU B   121                                                      
REMARK 465     GLU B   122                                                      
REMARK 465     HIS B   123                                                      
REMARK 465     HIS B   124                                                      
REMARK 465     HIS B   125                                                      
REMARK 465     HIS B   126                                                      
REMARK 465     HIS B   127                                                      
REMARK 465     HIS B   128                                                      
REMARK 465     MET C    31                                                      
REMARK 465     GLY C    32                                                      
REMARK 465     HIS C    33                                                      
REMARK 465     HIS C    34                                                      
REMARK 465     HIS C    35                                                      
REMARK 465     HIS C    36                                                      
REMARK 465     HIS C    37                                                      
REMARK 465     HIS C    38                                                      
REMARK 465     GLY C    39                                                      
REMARK 465     SER C    40                                                      
REMARK 465     LYS C    41                                                      
REMARK 465     ALA C    42                                                      
REMARK 465     ASN C    43                                                      
REMARK 465     LYS C    44                                                      
REMARK 465     GLU C    45                                                      
REMARK 465     PHE C    46                                                      
REMARK 465     GLY C    47                                                      
REMARK 465     ASP C    48                                                      
REMARK 465     SER C    49                                                      
REMARK 465     PHE C    92                                                      
REMARK 465     LYS C    93                                                      
REMARK 465     MET C    94                                                      
REMARK 465     GLY C    95                                                      
REMARK 465     ASN C    96                                                      
REMARK 465     ARG C    97                                                      
REMARK 465     HIS C    98                                                      
REMARK 465     LYS C    99                                                      
REMARK 465     PHE C   100                                                      
REMARK 465     THR C   101                                                      
REMARK 465     GLY C   102                                                      
REMARK 465     LYS C   103                                                      
REMARK 465     LYS C   104                                                      
REMARK 465     VAL C   105                                                      
REMARK 465     THR C   106                                                      
REMARK 465     GLU C   107                                                      
REMARK 465     ASP C   245                                                      
REMARK 465     GLN C   246                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LYS B 116    CG   CD   CE   NZ                                   
REMARK 470     THR B 117    OG1  CG2                                            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O2'    U A   195     NH2  ARG B    74              2.10            
REMARK 500   O2'    C A   161     O2'    A A   214              2.14            
REMARK 500   O2'    C A   212     OP2    A A   214              2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500      G A 164   N3  -  C4  -  C5  ANGL. DEV. =   3.2 DEGREES          
REMARK 500      C A 186   C6  -  N1  -  C1' ANGL. DEV. =  -7.5 DEGREES          
REMARK 500      C A 186   C2  -  N1  -  C1' ANGL. DEV. =   7.1 DEGREES          
REMARK 500      G A 235   C3' -  O3' -  P   ANGL. DEV. =   7.6 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ARG B  74        2.82    -66.53                                   
REMARK 500    LEU B 115      -77.20    -69.53                                   
REMARK 500    LEU C 109     -118.48     48.32                                   
REMARK 500    THR C 111      -63.55   -130.31                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue MG A 301                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue MG A 302                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue MG A 303                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue MG A 304                  
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 4P3F   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 4P3G   RELATED DB: PDB                                   
DBREF  4P3E A  115   236  GB     177793   M20910.1       235    356             
DBREF  4P3E B    1   120  UNP    P09132   SRP19_HUMAN      1    120             
DBREF  4P3E C   39   246  UNP    Q9UHB9   SRP68_HUMAN     47    254             
SEQADV 4P3E   G A  114  GB   177793              ENGINEERED MUTATION            
SEQADV 4P3E   U A  237  GB   177793              ENGINEERED MUTATION            
SEQADV 4P3E   A A  238  GB   177793              ENGINEERED MUTATION            
SEQADV 4P3E LEU B  121  UNP  P09132              EXPRESSION TAG                 
SEQADV 4P3E GLU B  122  UNP  P09132              EXPRESSION TAG                 
SEQADV 4P3E HIS B  123  UNP  P09132              EXPRESSION TAG                 
SEQADV 4P3E HIS B  124  UNP  P09132              EXPRESSION TAG                 
SEQADV 4P3E HIS B  125  UNP  P09132              EXPRESSION TAG                 
SEQADV 4P3E HIS B  126  UNP  P09132              EXPRESSION TAG                 
SEQADV 4P3E HIS B  127  UNP  P09132              EXPRESSION TAG                 
SEQADV 4P3E HIS B  128  UNP  P09132              EXPRESSION TAG                 
SEQADV 4P3E MET C   31  UNP  Q9UHB9              INITIATING METHIONINE          
SEQADV 4P3E GLY C   32  UNP  Q9UHB9              EXPRESSION TAG                 
SEQADV 4P3E HIS C   33  UNP  Q9UHB9              EXPRESSION TAG                 
SEQADV 4P3E HIS C   34  UNP  Q9UHB9              EXPRESSION TAG                 
SEQADV 4P3E HIS C   35  UNP  Q9UHB9              EXPRESSION TAG                 
SEQADV 4P3E HIS C   36  UNP  Q9UHB9              EXPRESSION TAG                 
SEQADV 4P3E HIS C   37  UNP  Q9UHB9              EXPRESSION TAG                 
SEQADV 4P3E HIS C   38  UNP  Q9UHB9              EXPRESSION TAG                 
SEQADV 4P3E ASP C  108  UNP  Q9UHB9    GLU   116 ENGINEERED MUTATION            
SEQRES   1 A  125    G   A   C   U   A   A   G   U   U   C   G   G   C          
SEQRES   2 A  125    A   U   C   A   A   U   A   U   G   G   U   G   A          
SEQRES   3 A  125    C   C   U   C   C   C   G   G   G   A   G   C   G          
SEQRES   4 A  125    G   G   G   G   A   C   C   A   C   C   A   G   G          
SEQRES   5 A  125    U   U   G   C   C   U   A   A   G   G   A   G   G          
SEQRES   6 A  125    G   G   U   G   A   A   C   C   G   G   C   C   C          
SEQRES   7 A  125    A   G   G   U   C   G   G   A   A   A   C   G   G          
SEQRES   8 A  125    A   G   C   A   G   G   U   C   A   A   A   A   C          
SEQRES   9 A  125    U   C   C   C   G   U   G   C   U   G   A   U   C          
SEQRES  10 A  125    A   G   U   A   G   U   U   A                              
SEQRES   1 B  128  MET ALA CYS ALA ALA ALA ARG SER PRO ALA ASP GLN ASP          
SEQRES   2 B  128  ARG PHE ILE CYS ILE TYR PRO ALA TYR LEU ASN ASN LYS          
SEQRES   3 B  128  LYS THR ILE ALA GLU GLY ARG ARG ILE PRO ILE SER LYS          
SEQRES   4 B  128  ALA VAL GLU ASN PRO THR ALA THR GLU ILE GLN ASP VAL          
SEQRES   5 B  128  CYS SER ALA VAL GLY LEU ASN VAL PHE LEU GLU LYS ASN          
SEQRES   6 B  128  LYS MET TYR SER ARG GLU TRP ASN ARG ASP VAL GLN TYR          
SEQRES   7 B  128  ARG GLY ARG VAL ARG VAL GLN LEU LYS GLN GLU ASP GLY          
SEQRES   8 B  128  SER LEU CYS LEU VAL GLN PHE PRO SER ARG LYS SER VAL          
SEQRES   9 B  128  MET LEU TYR ALA ALA GLU MET ILE PRO LYS LEU LYS THR          
SEQRES  10 B  128  ARG THR GLN LEU GLU HIS HIS HIS HIS HIS HIS                  
SEQRES   1 C  216  MET GLY HIS HIS HIS HIS HIS HIS GLY SER LYS ALA ASN          
SEQRES   2 C  216  LYS GLU PHE GLY ASP SER LEU SER LEU GLU ILE LEU GLN          
SEQRES   3 C  216  ILE ILE LYS GLU SER GLN GLN GLN HIS GLY LEU ARG HIS          
SEQRES   4 C  216  GLY ASP PHE GLN ARG TYR ARG GLY TYR CYS SER ARG ARG          
SEQRES   5 C  216  GLN ARG ARG LEU ARG LYS THR LEU ASN PHE LYS MET GLY          
SEQRES   6 C  216  ASN ARG HIS LYS PHE THR GLY LYS LYS VAL THR GLU ASP          
SEQRES   7 C  216  LEU LEU THR ASP ASN ARG TYR LEU LEU LEU VAL LEU MET          
SEQRES   8 C  216  ASP ALA GLU ARG ALA TRP SER TYR ALA MET GLN LEU LYS          
SEQRES   9 C  216  GLN GLU ALA ASN THR GLU PRO ARG LYS ARG PHE HIS LEU          
SEQRES  10 C  216  LEU SER ARG LEU ARG LYS ALA VAL LYS HIS ALA GLU GLU          
SEQRES  11 C  216  LEU GLU ARG LEU CYS GLU SER ASN ARG VAL ASP ALA LYS          
SEQRES  12 C  216  THR LYS LEU GLU ALA GLN ALA TYR THR ALA TYR LEU SER          
SEQRES  13 C  216  GLY MET LEU ARG PHE GLU HIS GLN GLU TRP LYS ALA ALA          
SEQRES  14 C  216  ILE GLU ALA PHE ASN LYS CYS LYS THR ILE TYR GLU LYS          
SEQRES  15 C  216  LEU ALA SER ALA PHE THR GLU GLU GLN ALA VAL LEU TYR          
SEQRES  16 C  216  ASN GLN ARG VAL GLU GLU ILE SER PRO ASN ILE ARG TYR          
SEQRES  17 C  216  CYS ALA TYR ASN ILE GLY ASP GLN                              
HET     MG  A 301       1                                                       
HET     MG  A 302       1                                                       
HET     MG  A 303       1                                                       
HET     MG  A 304       1                                                       
HETNAM      MG MAGNESIUM ION                                                    
FORMUL   4   MG    4(MG 2+)                                                     
HELIX    1 AA1 TYR B   19  ASN B   24  5                                   6    
HELIX    2 AA2 THR B   28  GLY B   32  5                                   5    
HELIX    3 AA3 THR B   45  ALA B   55  1                                  11    
HELIX    4 AA4 ASP B   75  ARG B   79  5                                   5    
HELIX    5 AA5 SER B  100  ILE B  112  1                                  13    
HELIX    6 AA6 PRO B  113  LEU B  115  5                                   3    
HELIX    7 AA7 GLU C   53  HIS C   65  1                                  13    
HELIX    8 AA8 ASP C   71  ASN C   91  1                                  21    
HELIX    9 AA9 ASN C  113  ALA C  137  1                                  25    
HELIX   10 AB1 GLU C  140  CYS C  165  1                                  26    
HELIX   11 AB2 ASP C  171  HIS C  193  1                                  23    
HELIX   12 AB3 GLU C  195  ALA C  216  1                                  22    
HELIX   13 AB4 THR C  218  TYR C  241  1                                  24    
SHEET    1 AA1 2 VAL B  60  GLU B  63  0                                        
SHEET    2 AA1 2 ARG B  81  VAL B  84 -1  O  ARG B  81   N  GLU B  63           
LINK         OP2   A A 163                MG    MG A 303     1555   1555  2.78  
SITE     1 AC1  1   A A 157                                                     
SITE     1 AC2  2   C A 126    A A 127                                          
SITE     1 AC3  2   A A 163    G A 164                                          
SITE     1 AC4  1   C A 145                                                     
CRYST1  125.649   70.530  106.493  90.00 100.46  90.00 C 1 2 1       4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.007959  0.000000  0.001470        0.00000                         
SCALE2      0.000000  0.014178  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.009549        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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