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Database: PDB
Entry: 4TZP
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Original site: 4TZP 
HEADER    RIBOSOMAL PROTEIN/RNA                   10-JUL-14   4TZP              
TITLE     AS GROWN, UNTREATED CO-CRYSTALS OF THE TERNARY COMPLEX CONTAINING A T-
TITLE    2 BOX STEM I RNA, ITS COGNATE TRNAGLY, AND B. SUBTILIS YBXF PROTEIN    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: RIBOSOME-ASSOCIATED PROTEIN L7AE-LIKE;                     
COMPND   3 CHAIN: A, D;                                                         
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: ENGINEERED TRNA;                                           
COMPND   7 CHAIN: B, E;                                                         
COMPND   8 ENGINEERED: YES;                                                     
COMPND   9 MOL_ID: 3;                                                           
COMPND  10 MOLECULE: GLYQ T-BOX STEM I;                                         
COMPND  11 CHAIN: C, F;                                                         
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: BACILLUS SUBTILIS;                              
SOURCE   3 ORGANISM_TAXID: 1423;                                                
SOURCE   4 GENE: RPLGB, YBAB, YBXF, BSU01090;                                   
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 SYNTHETIC: YES;                                                      
SOURCE  11 ORGANISM_SCIENTIFIC: BACILLUS SUBTILIS;                              
SOURCE  12 ORGANISM_TAXID: 1423;                                                
SOURCE  13 MOL_ID: 3;                                                           
SOURCE  14 SYNTHETIC: YES;                                                      
SOURCE  15 ORGANISM_SCIENTIFIC: OCEANOBACILLUS IHEYENSIS;                       
SOURCE  16 ORGANISM_TAXID: 182710                                               
KEYWDS    RNA, RIBOSWITCH, T-BOX, TRNA, RIBOSOMAL PROTEIN-RNA COMPLEX           
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.ZHANG,A.R.FERRE-D'AMARE                                             
REVDAT   2   24-SEP-14 4TZP    1       JRNL                                     
REVDAT   1   10-SEP-14 4TZP    0                                                
JRNL        AUTH   J.ZHANG,A.R.FERRE-D'AMARE                                    
JRNL        TITL   DRAMATIC IMPROVEMENT OF CRYSTALS OF LARGE RNAS BY CATION     
JRNL        TITL 2 REPLACEMENT AND DEHYDRATION.                                 
JRNL        REF    STRUCTURE                     V.  22  1363 2014              
JRNL        REFN                   ISSN 0969-2126                               
JRNL        PMID   25185828                                                     
JRNL        DOI    10.1016/J.STR.2014.07.011                                    
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   J.ZHANG,A.R.FERRE-D'AMARE                                    
REMARK   1  TITL   CO-CRYSTAL STRUCTURE OF A T-BOX RIBOSWITCH STEM I DOMAIN IN  
REMARK   1  TITL 2 COMPLEX WITH ITS COGNATE TRNA.                               
REMARK   1  REF    NATURE                        V. 500   363 2013              
REMARK   1  REFN                   ESSN 1476-4687                               
REMARK   1  PMID   23892783                                                     
REMARK   1  DOI    10.1038/NATURE12440                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    8.50 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.8.4_1496)                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 8.50                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 48.63                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 1648                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.278                           
REMARK   3   R VALUE            (WORKING SET) : 0.267                           
REMARK   3   FREE R VALUE                     : 0.373                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 9.710                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 160                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 48.6300 -  8.5030    1.00     1488   160  0.2673 0.3733        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 1.110            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 47.070           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.004           9404                                  
REMARK   3   ANGLE     :  0.818          14456                                  
REMARK   3   CHIRALITY :  0.039           1938                                  
REMARK   3   PLANARITY :  0.005            525                                  
REMARK   3   DIHEDRAL  : 15.878           4507                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 36                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 2 THROUGH 29 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):  -9.6546 -11.2382 -54.8900              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  10.5677 T22:   6.9382                                     
REMARK   3      T33:  11.7460 T12:   4.4022                                     
REMARK   3      T13:   1.6390 T23:   0.8082                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0769 L22:   0.1090                                     
REMARK   3      L33:   0.1649 L12:   0.0842                                     
REMARK   3      L13:   0.0577 L23:   0.0078                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3181 S12:  -0.8314 S13:   0.8797                       
REMARK   3      S21:   0.4665 S22:  -0.0339 S23:   0.7429                       
REMARK   3      S31:  -0.1589 S32:  -0.5372 S33:  -0.0000                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 30 THROUGH 38 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -4.7177  -2.1248 -52.6509              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   5.4382 T22:   9.6601                                     
REMARK   3      T33:   8.9883 T12:   1.7171                                     
REMARK   3      T13:  -2.0974 T23:   0.3230                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0535 L22:   0.0173                                     
REMARK   3      L33:   0.0079 L12:  -0.0245                                     
REMARK   3      L13:  -0.0156 L23:   0.0334                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2929 S12:   0.0979 S13:   0.0225                       
REMARK   3      S21:   0.3390 S22:   0.2644 S23:  -0.1309                       
REMARK   3      S31:  -0.0268 S32:  -0.1534 S33:   0.0001                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 39 THROUGH 52 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):   2.7149  -7.0116 -55.3678              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  12.9814 T22:  10.4103                                     
REMARK   3      T33:   6.1551 T12:  -0.5666                                     
REMARK   3      T13:  -2.0092 T23:  -0.7457                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0267 L22:   0.0621                                     
REMARK   3      L33:   0.0487 L12:   0.0252                                     
REMARK   3      L13:  -0.0290 L23:  -0.0687                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.4355 S12:   0.2837 S13:   0.3987                       
REMARK   3      S21:   0.4882 S22:   0.7112 S23:  -0.0146                       
REMARK   3      S31:   0.0049 S32:  -0.3454 S33:  -0.0000                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 53 THROUGH 63 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -5.6785  -1.2438 -54.4841              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.8071 T22:   5.4759                                     
REMARK   3      T33:   4.3200 T12:   0.7731                                     
REMARK   3      T13:   0.3300 T23:  -0.5966                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.9104 L22:   0.1279                                     
REMARK   3      L33:   1.9862 L12:  -1.3865                                     
REMARK   3      L13:  -5.7682 L23:   0.6239                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0274 S12:   0.7984 S13:   0.1882                       
REMARK   3      S21:  -1.6070 S22:  -0.4386 S23:  -0.5476                       
REMARK   3      S31:  -1.4996 S32:   0.6323 S33:  -0.4330                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 64 THROUGH 82 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -12.4798  -6.3163 -49.8375              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  10.0961 T22:   4.9656                                     
REMARK   3      T33:   1.9138 T12:   0.1016                                     
REMARK   3      T13:  -3.2463 T23:   0.5565                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.3730 L22:   2.4685                                     
REMARK   3      L33:   3.7363 L12:   1.5088                                     
REMARK   3      L13:  -0.3208 L23:  -1.9531                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.5177 S12:   0.3631 S13:  -1.4219                       
REMARK   3      S21:  -0.3048 S22:  -0.1354 S23:  -0.6553                       
REMARK   3      S31:   0.2093 S32:   0.0924 S33:  -0.1453                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 5 THROUGH 15 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -14.8780  -7.9700   9.5885              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   6.7204 T22:  11.6404                                     
REMARK   3      T33:   8.2183 T12:   3.8241                                     
REMARK   3      T13:  -2.0788 T23:  -0.9519                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.1543 L22:   0.0018                                     
REMARK   3      L33:   0.0284 L12:   0.1295                                     
REMARK   3      L13:   0.0819 L23:   0.0099                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.9696 S12:   0.1524 S13:   1.0117                       
REMARK   3      S21:   0.4291 S22:  -2.1661 S23:   0.5193                       
REMARK   3      S31:   0.4468 S32:   0.0998 S33:   0.0000                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 16 THROUGH 30 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -3.9191 -15.1160   4.3602              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   4.0358 T22:   2.7712                                     
REMARK   3      T33:   8.2014 T12:   5.6609                                     
REMARK   3      T13:  -3.7114 T23:  -1.4652                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -0.4761 L22:  -0.1908                                     
REMARK   3      L33:  -0.0360 L12:  -0.1806                                     
REMARK   3      L13:  -0.0743 L23:   0.2388                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.9446 S12:   7.2899 S13:  -2.0699                       
REMARK   3      S21:   5.2171 S22:   0.9760 S23:  -0.9837                       
REMARK   3      S31:  -0.6046 S32:   1.9864 S33:  -0.0000                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 31 THROUGH 51 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -1.6496 -12.3735  -2.8004              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   7.7223 T22:  11.0794                                     
REMARK   3      T33:   9.6208 T12:  -3.0148                                     
REMARK   3      T13:  -0.6089 T23:  -5.9667                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.6018 L22:   0.1893                                     
REMARK   3      L33:  -0.0477 L12:   0.4548                                     
REMARK   3      L13:   0.0792 L23:   0.2278                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   4.4475 S12:   1.0860 S13:  -0.0381                       
REMARK   3      S21:  -1.3970 S22:   3.4898 S23:   2.0348                       
REMARK   3      S31:   1.1765 S32:   0.7211 S33:   0.0000                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 52 THROUGH 79 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -16.8598  -0.8809  24.6212              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  18.0854 T22:   7.3685                                     
REMARK   3      T33:   6.4898 T12:  -2.2470                                     
REMARK   3      T13:  -4.9151 T23:   2.3733                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -0.0309 L22:   0.4292                                     
REMARK   3      L33:   0.2113 L12:   0.2395                                     
REMARK   3      L13:   0.2442 L23:  -0.0464                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1224 S12:  -0.9465 S13:  -1.1858                       
REMARK   3      S21:  -0.1487 S22:  -0.1117 S23:   0.9401                       
REMARK   3      S31:   5.4348 S32:  -0.8430 S33:   0.0000                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 1 THROUGH 10 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -25.6750 -11.0922 -33.3092              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   6.3872 T22:   4.4458                                     
REMARK   3      T33:   8.5891 T12:   2.0150                                     
REMARK   3      T13:   2.6747 T23:   2.7491                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0245 L22:  -0.1024                                     
REMARK   3      L33:  -0.0900 L12:  -0.0623                                     
REMARK   3      L13:   0.0586 L23:  -0.2419                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.9513 S12:   1.6286 S13:   1.0773                       
REMARK   3      S21:  -1.9908 S22:   2.4712 S23:  -1.9264                       
REMARK   3      S31:  -0.9847 S32:   2.3551 S33:  -0.0000                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 11 THROUGH 20 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -6.4903 -27.6639 -34.2528              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   7.4789 T22:   3.5243                                     
REMARK   3      T33:  10.6365 T12:  -0.9760                                     
REMARK   3      T13:   5.1859 T23:  -1.2706                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0358 L22:   0.0988                                     
REMARK   3      L33:   0.0690 L12:  -0.0114                                     
REMARK   3      L13:  -0.2113 L23:  -0.1043                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2606 S12:  -0.2237 S13:   0.2256                       
REMARK   3      S21:  -0.3407 S22:   0.6403 S23:  -0.5108                       
REMARK   3      S31:  -0.7703 S32:   0.6258 S33:   0.0000                       
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 21 THROUGH 33 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):   9.7403 -38.6115  -9.2274              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  10.4104 T22:  11.2066                                     
REMARK   3      T33:   3.4012 T12:   8.4883                                     
REMARK   3      T13:  -4.3943 T23:   8.6627                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0348 L22:   0.0130                                     
REMARK   3      L33:   0.0345 L12:  -0.0814                                     
REMARK   3      L13:   0.0640 L23:  -0.2325                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.6699 S12:  -1.1715 S13:   0.2696                       
REMARK   3      S21:   2.3704 S22:  -0.0719 S23:  -2.5711                       
REMARK   3      S31:  -0.1795 S32:  -0.1529 S33:   0.0000                       
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 34 THROUGH 43 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):   5.4663 -30.3163  19.4964              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   7.3453 T22:  16.3191                                     
REMARK   3      T33:   5.9758 T12:  -2.9800                                     
REMARK   3      T13:   6.7144 T23:   0.6386                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.3820 L22:   0.0527                                     
REMARK   3      L33:   0.0943 L12:   0.4442                                     
REMARK   3      L13:   0.1479 L23:   0.1268                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.4985 S12:  -0.1821 S13:  -0.9520                       
REMARK   3      S21:   2.8785 S22:   1.6638 S23:   0.6973                       
REMARK   3      S31:   0.3209 S32:   0.6809 S33:   0.0000                       
REMARK   3   TLS GROUP : 14                                                     
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 44 THROUGH 50 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  10.9201 -23.3730  22.1007              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   3.8524 T22:  11.3379                                     
REMARK   3      T33:  13.7104 T12:   0.4718                                     
REMARK   3      T13:   1.5340 T23:  -0.7428                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0942 L22:   0.2477                                     
REMARK   3      L33:   0.1394 L12:  -0.0636                                     
REMARK   3      L13:   0.2303 L23:  -0.2324                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.4037 S12:  -0.7212 S13:   0.4470                       
REMARK   3      S21:   1.5611 S22:  -2.6144 S23:   0.7949                       
REMARK   3      S31:   0.6104 S32:  -2.7531 S33:  -0.0000                       
REMARK   3   TLS GROUP : 15                                                     
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 51 THROUGH 65 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  11.6491 -15.7361  27.6825              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   4.8563 T22:   8.7783                                     
REMARK   3      T33:   3.3334 T12:  -1.7624                                     
REMARK   3      T13:  -0.0015 T23:   0.4883                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.1314 L22:  -0.0734                                     
REMARK   3      L33:  -0.1228 L12:   0.0486                                     
REMARK   3      L13:   0.0310 L23:  -0.0245                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   1.8626 S12:   0.3973 S13:   1.7692                       
REMARK   3      S21:   1.6215 S22:  -1.0556 S23:  -3.4010                       
REMARK   3      S31:  -0.3222 S32:   0.1731 S33:  -0.0000                       
REMARK   3   TLS GROUP : 16                                                     
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 66 THROUGH 74 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  15.5725 -28.2531   9.6164              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   4.8993 T22:   2.9417                                     
REMARK   3      T33:   4.6080 T12:   8.0660                                     
REMARK   3      T13:  -0.4728 T23:  -2.6837                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -0.0013 L22:   0.0226                                     
REMARK   3      L33:   0.1382 L12:  -0.0597                                     
REMARK   3      L13:   0.0050 L23:   0.1062                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1303 S12:   0.2631 S13:   0.7347                       
REMARK   3      S21:   0.8812 S22:  -1.2671 S23:   1.0741                       
REMARK   3      S31:  -3.3564 S32:  -0.0639 S33:  -0.0000                       
REMARK   3   TLS GROUP : 17                                                     
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 75 THROUGH 95 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):   0.0908 -30.3023 -25.5071              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  10.3514 T22:   3.3154                                     
REMARK   3      T33:  11.0528 T12:  -1.2729                                     
REMARK   3      T13:   3.8842 T23:   0.5401                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -1.1649 L22:   0.1976                                     
REMARK   3      L33:  -0.1502 L12:  -0.3156                                     
REMARK   3      L13:   1.0163 L23:   0.8361                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0212 S12:   1.1212 S13:  -0.3622                       
REMARK   3      S21:   1.7110 S22:  -0.7613 S23:   0.6304                       
REMARK   3      S31:   0.8593 S32:  -1.0300 S33:   0.0000                       
REMARK   3   TLS GROUP : 18                                                     
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 96 THROUGH 102 )                  
REMARK   3    ORIGIN FOR THE GROUP (A): -27.6948 -18.0076 -31.0790              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  11.6893 T22:  14.8740                                     
REMARK   3      T33:   8.0766 T12:  -4.3952                                     
REMARK   3      T13:  -2.4694 T23:   1.3965                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.2063 L22:  -0.1483                                     
REMARK   3      L33:   0.0960 L12:   0.0479                                     
REMARK   3      L13:   0.2022 L23:  -0.0197                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.7777 S12:   0.3503 S13:   0.3063                       
REMARK   3      S21:  -0.2798 S22:  -0.4906 S23:   1.7038                       
REMARK   3      S31:  -0.5570 S32:   0.4136 S33:  -0.0000                       
REMARK   3   TLS GROUP : 19                                                     
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 2 THROUGH 15 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -39.3097  14.0597 131.6411              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   5.4043 T22:   8.2913                                     
REMARK   3      T33:   4.2680 T12:  -2.3239                                     
REMARK   3      T13:  -0.1780 T23:   0.4361                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -0.0012 L22:   0.5487                                     
REMARK   3      L33:   0.7227 L12:  -0.0054                                     
REMARK   3      L13:  -0.0513 L23:   0.6751                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.4476 S12:  -0.1698 S13:   0.6138                       
REMARK   3      S21:   0.0106 S22:  -0.3001 S23:  -0.0126                       
REMARK   3      S31:  -0.5257 S32:  -0.0048 S33:  -0.2041                       
REMARK   3   TLS GROUP : 20                                                     
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 16 THROUGH 38 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -40.7521   5.8088 124.0975              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   4.6619 T22:   7.6961                                     
REMARK   3      T33:  13.1801 T12:  -5.8103                                     
REMARK   3      T13:   1.1954 T23:  -1.4774                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0915 L22:   2.7752                                     
REMARK   3      L33:   0.0526 L12:  -0.3906                                     
REMARK   3      L13:   0.0503 L23:  -0.1454                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.5133 S12:   1.1122 S13:  -0.8484                       
REMARK   3      S21:   0.3054 S22:  -1.2272 S23:   0.6662                       
REMARK   3      S31:   0.4378 S32:   0.2670 S33:   0.0038                       
REMARK   3   TLS GROUP : 21                                                     
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 39 THROUGH 57 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -42.9142  -0.7392 125.8095              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   6.1726 T22:   5.9863                                     
REMARK   3      T33:   8.5644 T12:   2.3201                                     
REMARK   3      T13:   3.8192 T23:   0.1297                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -0.0141 L22:   0.0149                                     
REMARK   3      L33:   0.0013 L12:   0.0025                                     
REMARK   3      L13:  -0.0070 L23:   0.0001                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3319 S12:   0.3805 S13:   0.0639                       
REMARK   3      S21:  -0.0022 S22:  -0.0768 S23:  -0.1905                       
REMARK   3      S31:  -0.3794 S32:  -0.4706 S33:  -0.0000                       
REMARK   3   TLS GROUP : 22                                                     
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 58 THROUGH 63 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -51.0930   8.3533 132.1425              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  11.7116 T22:   7.9670                                     
REMARK   3      T33:   6.6191 T12:   2.6466                                     
REMARK   3      T13:   1.3480 T23:   1.3403                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0276 L22:   0.1057                                     
REMARK   3      L33:   0.0841 L12:  -0.0517                                     
REMARK   3      L13:  -0.0087 L23:  -0.0448                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3183 S12:  -0.0473 S13:   0.3207                       
REMARK   3      S21:  -0.0958 S22:  -0.1707 S23:   0.4016                       
REMARK   3      S31:  -0.0509 S32:  -0.1127 S33:  -0.0004                       
REMARK   3   TLS GROUP : 23                                                     
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 64 THROUGH 82 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -44.3728  14.1460 128.4520              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   8.2918 T22:   4.9187                                     
REMARK   3      T33:  11.2225 T12:  -0.2724                                     
REMARK   3      T13:  -1.7139 T23:   2.9568                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0438 L22:   0.1423                                     
REMARK   3      L33:   0.0190 L12:   0.1178                                     
REMARK   3      L13:  -0.0397 L23:  -0.0098                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3777 S12:   0.5622 S13:  -0.2605                       
REMARK   3      S21:  -0.1219 S22:   0.0860 S23:  -0.9066                       
REMARK   3      S31:  -0.2601 S32:   0.7412 S33:  -0.0010                       
REMARK   3   TLS GROUP : 24                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 5 THROUGH 15 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -46.5120   8.6483  65.9883              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   7.6730 T22:   8.9713                                     
REMARK   3      T33:  11.8548 T12:  -2.9467                                     
REMARK   3      T13:  -2.1516 T23:   4.7389                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -0.0315 L22:  -0.0267                                     
REMARK   3      L33:   0.0198 L12:   0.0652                                     
REMARK   3      L13:   0.1532 L23:  -0.0008                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0396 S12:  -0.3712 S13:  -1.0789                       
REMARK   3      S21:   0.6395 S22:  -0.5685 S23:   0.4008                       
REMARK   3      S31:  -0.1430 S32:   0.4776 S33:   0.0000                       
REMARK   3   TLS GROUP : 25                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 16 THROUGH 30 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -39.3905  -2.7567  70.3986              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   9.9892 T22:   9.7824                                     
REMARK   3      T33:  11.8461 T12:   2.7139                                     
REMARK   3      T13:   2.6015 T23:  -1.3564                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0912 L22:   0.1112                                     
REMARK   3      L33:   0.0371 L12:  -0.3104                                     
REMARK   3      L13:  -0.1139 L23:   0.0827                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.8369 S12:  -2.7736 S13:  -1.2182                       
REMARK   3      S21:   4.8489 S22:  -0.8870 S23:   1.0896                       
REMARK   3      S31:   1.7524 S32:   1.2688 S33:   0.0000                       
REMARK   3   TLS GROUP : 26                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 31 THROUGH 51 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -42.2031  -5.5844  77.3354              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  13.9588 T22:   9.6479                                     
REMARK   3      T33:  18.8162 T12:  -1.5018                                     
REMARK   3      T13:   0.5452 T23:   5.3202                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.3965 L22:   1.4149                                     
REMARK   3      L33:  -0.0918 L12:   0.2098                                     
REMARK   3      L13:   0.2060 L23:   0.2644                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -3.1126 S12:  -5.0253 S13:  -0.2961                       
REMARK   3      S21:   0.1523 S22:   3.6425 S23:   0.8824                       
REMARK   3      S31:   2.9917 S32:   3.9914 S33:  -0.0000                       
REMARK   3   TLS GROUP : 27                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 52 THROUGH 60 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -47.4541   0.7163  46.4867              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   5.2470 T22:   2.3448                                     
REMARK   3      T33:   6.0997 T12:   0.7696                                     
REMARK   3      T13:  -0.2495 T23:  -2.5048                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -0.0218 L22:  -0.0099                                     
REMARK   3      L33:  -0.0556 L12:   0.0194                                     
REMARK   3      L13:   0.0809 L23:   0.0405                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.5891 S12:   0.0203 S13:   0.5243                       
REMARK   3      S21:   0.3896 S22:  -0.8540 S23:  -1.4042                       
REMARK   3      S31:  -0.2022 S32:   0.7028 S33:  -0.0000                       
REMARK   3   TLS GROUP : 28                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 61 THROUGH 68 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -58.8593  13.7186  54.5724              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   7.3478 T22:   8.7289                                     
REMARK   3      T33:  12.5193 T12:   4.4994                                     
REMARK   3      T13:   1.8140 T23:  12.6588                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0390 L22:   0.0518                                     
REMARK   3      L33:   0.0048 L12:  -0.0699                                     
REMARK   3      L13:  -0.0483 L23:  -0.1426                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.7590 S12:  -0.1789 S13:   1.0899                       
REMARK   3      S21:  -1.7828 S22:  -1.6460 S23:  -1.2649                       
REMARK   3      S31:   0.8578 S32:   0.2725 S33:  -0.0000                       
REMARK   3   TLS GROUP : 29                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 69 THROUGH 79 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -60.2099  28.2133  51.7574              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   5.9080 T22:   9.7274                                     
REMARK   3      T33:  13.0679 T12:  -1.4547                                     
REMARK   3      T13:   4.4713 T23:  -5.1285                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.1868 L22:   0.0220                                     
REMARK   3      L33:   0.0690 L12:   0.1063                                     
REMARK   3      L13:  -0.1809 L23:   0.1622                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.8232 S12:   0.2184 S13:  -0.7629                       
REMARK   3      S21:  -0.5324 S22:   0.0162 S23:   0.6645                       
REMARK   3      S31:  -0.2983 S32:  -0.0744 S33:  -0.0000                       
REMARK   3   TLS GROUP : 30                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 1 THROUGH 13 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -36.7904  15.2106 108.6894              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   5.9823 T22:   2.2892                                     
REMARK   3      T33:   4.6030 T12:  -9.6539                                     
REMARK   3      T13:   0.9416 T23:  21.8729                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -0.1138 L22:  -0.1118                                     
REMARK   3      L33:  -0.1132 L12:  -0.1594                                     
REMARK   3      L13:  -0.2878 L23:  -0.3477                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   2.9525 S12:  -1.3649 S13:  -1.2233                       
REMARK   3      S21:  -0.5992 S22:   0.4005 S23:   0.8288                       
REMARK   3      S31:   1.0655 S32:  -1.6673 S33:   0.0000                       
REMARK   3   TLS GROUP : 31                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 14 THROUGH 23 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -19.7698  -8.3792 100.8152              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   7.5044 T22:   6.0047                                     
REMARK   3      T33:   6.0142 T12:  -6.9376                                     
REMARK   3      T13:  -1.1572 T23:   2.7174                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.1043 L22:   0.1126                                     
REMARK   3      L33:   0.0845 L12:   0.1061                                     
REMARK   3      L13:  -0.0483 L23:  -0.1409                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   1.6222 S12:   1.5610 S13:   1.6324                       
REMARK   3      S21:   1.1160 S22:   0.4900 S23:   1.3846                       
REMARK   3      S31:  -0.4818 S32:   0.1871 S33:  -0.0000                       
REMARK   3   TLS GROUP : 32                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 24 THROUGH 38 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -16.1569 -17.8293  72.4868              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   9.0223 T22:   7.6372                                     
REMARK   3      T33:   6.2920 T12:   4.9970                                     
REMARK   3      T13:  -0.4646 T23:   0.6414                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  -0.0743 L22:   0.0340                                     
REMARK   3      L33:  -0.1040 L12:  -0.0679                                     
REMARK   3      L13:   0.3319 L23:   0.2169                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -2.3077 S12:   0.3358 S13:  -4.8505                       
REMARK   3      S21:  -1.9705 S22:  -2.9769 S23:   0.5626                       
REMARK   3      S31:   0.1458 S32:   1.3695 S33:   0.0000                       
REMARK   3   TLS GROUP : 33                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 39 THROUGH 57 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -32.2989 -14.7822  45.4442              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   5.3314 T22:  13.7564                                     
REMARK   3      T33:   2.8034 T12:   2.7179                                     
REMARK   3      T13:   1.7754 T23:   4.5895                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.0320 L22:  -0.0377                                     
REMARK   3      L33:   0.3101 L12:   0.2998                                     
REMARK   3      L13:  -0.2787 L23:  -0.2341                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -4.8326 S12:   4.1001 S13:   1.6468                       
REMARK   3      S21:   1.8140 S22:  -3.4542 S23:  -3.0840                       
REMARK   3      S31:  -1.8928 S32:   0.7274 S33:  -0.0000                       
REMARK   3   TLS GROUP : 34                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 58 THROUGH 74 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -29.1091 -18.5992  53.6570              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   4.6187 T22:  11.9570                                     
REMARK   3      T33:   3.0410 T12:  -0.6168                                     
REMARK   3      T13:  -0.6355 T23:   3.1200                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.5655 L22:   0.3409                                     
REMARK   3      L33:   0.1367 L12:  -0.0984                                     
REMARK   3      L13:  -0.3411 L23:  -0.0158                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.6582 S12:  -0.5250 S13:   2.7097                       
REMARK   3      S21:   1.6820 S22:   5.5833 S23:   3.6975                       
REMARK   3      S31:   0.2941 S32:   0.1690 S33:   0.0000                       
REMARK   3   TLS GROUP : 35                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 75 THROUGH 92 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -19.7052 -10.9214  93.8685              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  13.9983 T22:   5.5281                                     
REMARK   3      T33:  10.9011 T12:  -2.3779                                     
REMARK   3      T13:  -6.6166 T23:   2.2851                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.5888 L22:   0.3378                                     
REMARK   3      L33:  -0.0727 L12:   0.5310                                     
REMARK   3      L13:   0.5401 L23:   0.2563                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   4.0085 S12:  -1.1152 S13:  -1.5663                       
REMARK   3      S21:  -0.2627 S22:  -1.1537 S23:  -3.5787                       
REMARK   3      S31:   3.2786 S32:   2.8215 S33:  -0.0000                       
REMARK   3   TLS GROUP : 36                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 93 THROUGH 102 )                  
REMARK   3    ORIGIN FOR THE GROUP (A): -30.5831  13.8352 109.4776              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:  11.9753 T22:  17.0523                                     
REMARK   3      T33:  17.5492 T12:  -1.8580                                     
REMARK   3      T13:  -9.2388 T23:   7.2594                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.5626 L22:   0.3072                                     
REMARK   3      L33:   0.3100 L12:   0.3850                                     
REMARK   3      L13:   0.3673 L23:   0.5396                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   1.2193 S12:   2.5186 S13:   0.5412                       
REMARK   3      S21:   2.1104 S22:   0.1557 S23:  -0.9186                       
REMARK   3      S31:   2.5221 S32:  -0.9503 S33:  -0.0000                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 4TZP COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 16-JUL-14.                  
REMARK 100 THE DEPOSITION ID IS D_1000202574.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 13-AUG-13                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.5-7.0                            
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ALS                                
REMARK 200  BEAMLINE                       : 5.0.2                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9793                             
REMARK 200  MONOCHROMATOR                  : KOHZU HLD8-24 MONOCHROMATOR        
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : NULL                               
REMARK 200  DATA SCALING SOFTWARE          : NULL                               
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 1651                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 8.500                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 48.630                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 6.700                              
REMARK 200  R MERGE                    (I) : 0.08300                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 10.6000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 8.50                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 6.70                               
REMARK 200  R MERGE FOR SHELL          (I) : 1.08000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.700                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: NULL                                                  
REMARK 200 STARTING MODEL: 4LCK                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 62.95                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.32                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 50 MM BIS-TRIS (HCL) PH 6.5, 300 MM      
REMARK 280  LI2SO4, AND 20% (W/V) PEG3350, VAPOR DIFFUSION, HANGING DROP,       
REMARK 280  TEMPERATURE 293K                                                    
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -X,Y,-Z+1/2                                             
REMARK 290       4555   X,-Y,-Z                                                 
REMARK 290       5555   X+1/2,Y+1/2,Z                                           
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2                                     
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2                                     
REMARK 290       8555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      145.90550            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000      145.90550            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       54.36500            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       54.37450            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       54.36500            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       54.37450            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000      145.90550            
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       54.36500            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       54.37450            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000      145.90550            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       54.36500            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       54.37450            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC                          
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D, E, F                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A     1                                                      
REMARK 465       G B    72                                                      
REMARK 465       A B    73                                                      
REMARK 465       A B    74                                                      
REMARK 465       A B    75                                                      
REMARK 465       G B    76                                                      
REMARK 465     GLY D     1                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ASP A   4    CG   OD1  OD2                                       
REMARK 470     LYS A   5    CG   CD   CE   NZ                                   
REMARK 470     LYS A  10    CG   CD   CE   NZ                                   
REMARK 470     LYS A  17    CG   CD   CE   NZ                                   
REMARK 470     LYS A  21    CD   CE   NZ                                        
REMARK 470     LYS A  29    CG   CD   CE   NZ                                   
REMARK 470     LYS A  35    CD   CE   NZ                                        
REMARK 470     ASP A  38    CG   OD1  OD2                                       
REMARK 470     LEU A  48    CG   CD1  CD2                                       
REMARK 470     ASP A  51    CG   OD1  OD2                                       
REMARK 470     GLU A  60    CG   CD   OE1  OE2                                  
REMARK 470     LYS A  63    CD   CE   NZ                                        
REMARK 470     LYS A  64    CD   CE   NZ                                        
REMARK 470     LYS A  67    CD   CE   NZ                                        
REMARK 470     ILE A  71    CG1  CG2  CD1                                       
REMARK 470     GLU A  72    CG   CD   OE1  OE2                                  
REMARK 470     ILE A  81    CG1  CG2  CD1                                       
REMARK 470       U B  19    N1   C2   O2   N3   C4   O4   C5                    
REMARK 470       U B  19    C6                                                  
REMARK 470       U B  46    N1   C2   O2   N3   C4   O4   C5                    
REMARK 470       U B  46    C6                                                  
REMARK 470     ASP D   4    CG   OD1  OD2                                       
REMARK 470     LYS D   5    CG   CD   CE   NZ                                   
REMARK 470     LYS D  10    CG   CD   CE   NZ                                   
REMARK 470     LYS D  17    CG   CD   CE   NZ                                   
REMARK 470     LYS D  21    CD   CE   NZ                                        
REMARK 470     LYS D  29    CG   CD   CE   NZ                                   
REMARK 470     LYS D  35    CD   CE   NZ                                        
REMARK 470     ASP D  38    CG   OD1  OD2                                       
REMARK 470     LEU D  48    CG   CD1  CD2                                       
REMARK 470     ASP D  51    CG   OD1  OD2                                       
REMARK 470     GLU D  60    CG   CD   OE1  OE2                                  
REMARK 470     LYS D  63    CD   CE   NZ                                        
REMARK 470     LYS D  64    CD   CE   NZ                                        
REMARK 470     LYS D  67    CD   CE   NZ                                        
REMARK 470     ILE D  71    CG1  CG2  CD1                                       
REMARK 470     GLU D  72    CG   CD   OE1  OE2                                  
REMARK 470     ILE D  81    CG1  CG2  CD1                                       
REMARK 470       U E  19    N1   C2   O2   N3   C4   O4   C5                    
REMARK 470       U E  19    C6                                                  
REMARK 470       U E  46    N1   C2   O2   N3   C4   O4   C5                    
REMARK 470       U E  46    C6                                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    LYS D    21     NH1  ARG D    25              1.81            
REMARK 500   O    THR D    16     OG1  THR D    19              1.90            
REMARK 500   NH1  ARG D    25     N7     A F    96              2.00            
REMARK 500   N    LYS D    24     O6     G F    10              2.08            
REMARK 500   NE2  GLN A    18     OP2    U C    93              2.09            
REMARK 500   NZ   LYS D    24     OP2    G F    10              2.13            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500      A F   8   C5'     A F   8   C4'    -0.044                       
REMARK 500      G F  95   N9      G F  95   C4      0.082                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500      C B  77   C4  -  C5  -  C6  ANGL. DEV. =   4.2 DEGREES          
REMARK 500      U C   9   O4' -  C1' -  N1  ANGL. DEV. =   4.7 DEGREES          
REMARK 500      U C   9   C6  -  N1  -  C2  ANGL. DEV. =  -5.7 DEGREES          
REMARK 500      U C   9   C5  -  C6  -  N1  ANGL. DEV. =   5.6 DEGREES          
REMARK 500      U C   9   N1  -  C2  -  O2  ANGL. DEV. =   4.3 DEGREES          
REMARK 500      U C   9   N3  -  C2  -  O2  ANGL. DEV. =  -4.2 DEGREES          
REMARK 500      G C  10   O3' -  P   -  OP1 ANGL. DEV. =   8.3 DEGREES          
REMARK 500    LYS D  17   N   -  CA  -  C   ANGL. DEV. =  16.5 DEGREES          
REMARK 500      G E  76   OP1 -  P   -  OP2 ANGL. DEV. = -10.1 DEGREES          
REMARK 500      A F   8   O3' -  P   -  O5' ANGL. DEV. = -11.5 DEGREES          
REMARK 500      A F   8   N9  -  C1' -  C2' ANGL. DEV. =  14.3 DEGREES          
REMARK 500      A F   8   N9  -  C4  -  C5  ANGL. DEV. =   3.0 DEGREES          
REMARK 500      G F  10   C2  -  N3  -  C4  ANGL. DEV. =  -3.0 DEGREES          
REMARK 500      G F  10   C8  -  N9  -  C4  ANGL. DEV. =   3.6 DEGREES          
REMARK 500      G F  10   N9  -  C4  -  C5  ANGL. DEV. =  -3.2 DEGREES          
REMARK 500      G F  10   N1  -  C2  -  N2  ANGL. DEV. = -10.3 DEGREES          
REMARK 500      G F  10   N3  -  C2  -  N2  ANGL. DEV. =   8.6 DEGREES          
REMARK 500      G F  10   N1  -  C6  -  O6  ANGL. DEV. =  -7.0 DEGREES          
REMARK 500      G F  10   C5  -  C6  -  O6  ANGL. DEV. =   6.7 DEGREES          
REMARK 500      G F  95   C2  -  N3  -  C4  ANGL. DEV. =   3.7 DEGREES          
REMARK 500      G F  95   N3  -  C4  -  C5  ANGL. DEV. =  -6.9 DEGREES          
REMARK 500      G F  95   C8  -  N9  -  C4  ANGL. DEV. =  -3.4 DEGREES          
REMARK 500      G F  95   N3  -  C4  -  N9  ANGL. DEV. =   7.5 DEGREES          
REMARK 500      G F  95   N1  -  C2  -  N2  ANGL. DEV. =  -5.7 DEGREES          
REMARK 500      G F  95   N3  -  C2  -  N2  ANGL. DEV. =   6.3 DEGREES          
REMARK 500      G F  95   N1  -  C6  -  O6  ANGL. DEV. =  -6.0 DEGREES          
REMARK 500      G F  95   C5  -  C6  -  O6  ANGL. DEV. =   4.5 DEGREES          
REMARK 500      G F  95   C8  -  N9  -  C1' ANGL. DEV. = -15.4 DEGREES          
REMARK 500      G F  95   C4  -  N9  -  C1' ANGL. DEV. =  17.9 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    GLN A   8     -153.95    -97.28                                   
REMARK 500    ALA A   9     -150.77     66.50                                   
REMARK 500    LYS A  10      -70.97   -132.66                                   
REMARK 500    VAL A  73      -67.68   -133.71                                   
REMARK 500    LYS D  10      -71.90    -87.01                                   
REMARK 500    GLU D  60      -71.16    -62.37                                   
REMARK 500    VAL D  73      -67.08   -133.06                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 VAL A   73     GLY A   74                 -145.62                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 4LCK   RELATED DB: PDB                                   
REMARK 900 4LCK CONTAINS THE SAME COMPLEX BUT SUBJECTED TO POST-                
REMARK 900 CRYSTALLIZATION TREATMENTS                                           
REMARK 900 RELATED ID: 4TZV   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 4TZW   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 4TZX   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 4TZY   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 4TZZ   RELATED DB: PDB                                   
DBREF  4TZP A    2    82  UNP    P46350   RXL7_BACSU       2     82             
DBREF  4TZP B    5    79  PDB    4TZP     4TZP             5     79             
DBREF  4TZP C    1   102  PDB    4TZP     4TZP             1    102             
DBREF  4TZP D    2    82  UNP    P46350   RXL7_BACSU       2     82             
DBREF  4TZP E    5    79  PDB    4TZP     4TZP             5     79             
DBREF  4TZP F    1   102  PDB    4TZP     4TZP             1    102             
SEQADV 4TZP GLY A    1  UNP  P46350              EXPRESSION TAG                 
SEQADV 4TZP GLY D    1  UNP  P46350              EXPRESSION TAG                 
SEQRES   1 A   82  GLY SER TYR ASP LYS VAL SER GLN ALA LYS SER ILE ILE          
SEQRES   2 A   82  ILE GLY THR LYS GLN THR VAL LYS ALA LEU LYS ARG GLY          
SEQRES   3 A   82  SER VAL LYS GLU VAL VAL VAL ALA LYS ASP ALA ASP PRO          
SEQRES   4 A   82  ILE LEU THR SER SER VAL VAL SER LEU ALA GLU ASP GLN          
SEQRES   5 A   82  GLY ILE SER VAL SER MSE VAL GLU SER MSE LYS LYS LEU          
SEQRES   6 A   82  GLY LYS ALA CYS GLY ILE GLU VAL GLY ALA ALA ALA VAL          
SEQRES   7 A   82  ALA ILE ILE LEU                                              
SEQRES   1 B   75    G   A   G   U   A   G   U   U   C   A   G   U   G          
SEQRES   2 B   75    G   U   A   G   A   A   C   A   C   C   A   C   C          
SEQRES   3 B   75    U   U   G   C   C   A   A   G   G   U   G   G   G          
SEQRES   4 B   75    G   G   U   C   G   C   G   G   G   U   U   C   G          
SEQRES   5 B   75    A   A   U   C   C   C   G   U   C   U   C   G   G          
SEQRES   6 B   75    G   C   G   A   A   A   G   C   C   C                      
SEQRES   1 C  102    G   G   G   U   G   C   G   A   U   G   A   G   A          
SEQRES   2 C  102    A   G   A   A   G   A   G   U   A   U   U   A   A          
SEQRES   3 C  102    G   G   A   U   U   U   A   C   U   A   U   G   A          
SEQRES   4 C  102    U   U   A   G   C   G   A   C   U   C   U   A   G          
SEQRES   5 C  102    G   A   U   A   G   U   G   A   A   A   G   C   U          
SEQRES   6 C  102    A   G   A   G   G   A   U   A   G   U   A   A   C          
SEQRES   7 C  102    C   U   U   A   A   G   A   A   G   G   C   A   C          
SEQRES   8 C  102    U   U   C   G   A   G   C   A   C   C   C                  
SEQRES   1 D   82  GLY SER TYR ASP LYS VAL SER GLN ALA LYS SER ILE ILE          
SEQRES   2 D   82  ILE GLY THR LYS GLN THR VAL LYS ALA LEU LYS ARG GLY          
SEQRES   3 D   82  SER VAL LYS GLU VAL VAL VAL ALA LYS ASP ALA ASP PRO          
SEQRES   4 D   82  ILE LEU THR SER SER VAL VAL SER LEU ALA GLU ASP GLN          
SEQRES   5 D   82  GLY ILE SER VAL SER MSE VAL GLU SER MSE LYS LYS LEU          
SEQRES   6 D   82  GLY LYS ALA CYS GLY ILE GLU VAL GLY ALA ALA ALA VAL          
SEQRES   7 D   82  ALA ILE ILE LEU                                              
SEQRES   1 E   75    G   A   G   U   A   G   U   U   C   A   G   U   G          
SEQRES   2 E   75    G   U   A   G   A   A   C   A   C   C   A   C   C          
SEQRES   3 E   75    U   U   G   C   C   A   A   G   G   U   G   G   G          
SEQRES   4 E   75    G   G   U   C   G   C   G   G   G   U   U   C   G          
SEQRES   5 E   75    A   A   U   C   C   C   G   U   C   U   C   G   G          
SEQRES   6 E   75    G   C   G   A   A   A   G   C   C   C                      
SEQRES   1 F  102    G   G   G   U   G   C   G   A   U   G   A   G   A          
SEQRES   2 F  102    A   G   A   A   G   A   G   U   A   U   U   A   A          
SEQRES   3 F  102    G   G   A   U   U   U   A   C   U   A   U   G   A          
SEQRES   4 F  102    U   U   A   G   C   G   A   C   U   C   U   A   G          
SEQRES   5 F  102    G   A   U   A   G   U   G   A   A   A   G   C   U          
SEQRES   6 F  102    A   G   A   G   G   A   U   A   G   U   A   A   C          
SEQRES   7 F  102    C   U   U   A   A   G   A   A   G   G   C   A   C          
SEQRES   8 F  102    U   U   C   G   A   G   C   A   C   C   C                  
MODRES 4TZP MSE A   58  MET  MODIFIED RESIDUE                                   
MODRES 4TZP MSE A   62  MET  MODIFIED RESIDUE                                   
MODRES 4TZP MSE D   58  MET  MODIFIED RESIDUE                                   
MODRES 4TZP MSE D   62  MET  MODIFIED RESIDUE                                   
HET    MSE  A  58       8                                                       
HET    MSE  A  62       8                                                       
HET    MSE  D  58       8                                                       
HET    MSE  D  62       8                                                       
HETNAM     MSE SELENOMETHIONINE                                                 
FORMUL   1  MSE    4(C5 H11 N O2 SE)                                            
HELIX    1 AA1 SER A    2  GLN A    8  1                                   7    
HELIX    2 AA2 GLY A   15  LYS A   24  1                                  10    
HELIX    3 AA3 ASP A   38  GLY A   53  1                                  16    
HELIX    4 AA4 MSE A   62  GLY A   70  1                                   9    
HELIX    5 AA5 TYR D    3  GLN D    8  1                                   6    
HELIX    6 AA6 GLY D   15  LYS D   24  1                                  10    
HELIX    7 AA7 ASP D   38  GLY D   53  1                                  16    
HELIX    8 AA8 SER D   61  GLY D   70  1                                  10    
SHEET    1 AA1 4 ILE A  12  ILE A  14  0                                        
SHEET    2 AA1 4 ALA A  77  ILE A  80 -1  O  ALA A  79   N  ILE A  13           
SHEET    3 AA1 4 GLU A  30  ALA A  34 -1  N  VAL A  32   O  VAL A  78           
SHEET    4 AA1 4 VAL A  56  VAL A  59  1  O  VAL A  59   N  VAL A  33           
SHEET    1 AA2 4 SER D  11  ILE D  14  0                                        
SHEET    2 AA2 4 ALA D  77  ILE D  81 -1  O  ALA D  79   N  ILE D  13           
SHEET    3 AA2 4 GLU D  30  ALA D  34 -1  N  VAL D  32   O  VAL D  78           
SHEET    4 AA2 4 SER D  55  VAL D  59  1  O  SER D  55   N  VAL D  31           
LINK         C   SER A  57                 N   MSE A  58     1555   1555  1.33  
LINK         C   MSE A  58                 N   VAL A  59     1555   1555  1.33  
LINK         C   SER A  61                 N   MSE A  62     1555   1555  1.33  
LINK         C   MSE A  62                 N   LYS A  63     1555   1555  1.34  
LINK         C   SER D  57                 N   MSE D  58     1555   1555  1.33  
LINK         C   MSE D  58                 N   VAL D  59     1555   1555  1.33  
LINK         C   SER D  61                 N   MSE D  62     1555   1555  1.33  
LINK         C   MSE D  62                 N   LYS D  63     1555   1555  1.33  
CRYST1  108.730  108.749  291.811  90.00  90.00  90.00 C 2 2 21     16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009197  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.009195  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.003427        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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