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Database: PDB
Entry: 5EYO
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Original site: 5EYO 
HEADER    TRANSCRIPTION/DNA                       25-NOV-15   5EYO              
TITLE     THE CRYSTAL STRUCTURE OF THE MAX BHLH DOMAIN IN COMPLEX WITH 5-       
TITLE    2 CARBOXYL CYTOSINE DNA                                                
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN MAX;                                               
COMPND   3 CHAIN: A, C;                                                         
COMPND   4 FRAGMENT: UNP RESIDUES 22-107;                                       
COMPND   5 SYNONYM: CLASS D BASIC HELIX-LOOP-HELIX PROTEIN 4,BHLHD4,MYC-        
COMPND   6 ASSOCIATED FACTOR X;                                                 
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MOL_ID: 2;                                                           
COMPND   9 MOLECULE: DNA (5'-D(*AP*GP*TP*AP*GP*CP*AP*(1CC)                      
COMPND  10 P*GP*TP*GP*CP*TP*AP*CP*T)-3');                                       
COMPND  11 CHAIN: B, D;                                                         
COMPND  12 ENGINEERED: YES;                                                     
COMPND  13 OTHER_DETAILS: 1CC IS 5-CARBOXY-2'-DEOXYCYTIDINE MONOPHOSPHATE       
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 CELL_LINE: HEK293T CELLS;                                            
SOURCE   6 GENE: MAX, BHLHD4;                                                   
SOURCE   7 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   8 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   9 EXPRESSION_SYSTEM_STRAIN: BL21 (DE3) CODON PLUS;                     
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET-HIS-SUMO;                             
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 SYNTHETIC: YES;                                                      
SOURCE  13 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT;                            
SOURCE  14 ORGANISM_TAXID: 32630                                                
KEYWDS    BHLH PROTEIN, 5-CARBOXYLCYTOSINE DNA, TRANSCRIPTION-DNA COMPLEX       
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    D.WANG,H.HASHIMOTO,X.ZHANG,X.CHENG                                    
REVDAT   4   25-DEC-19 5EYO    1       REMARK                                   
REVDAT   3   13-SEP-17 5EYO    1       REMARK                                   
REVDAT   2   03-MAY-17 5EYO    1       JRNL                                     
REVDAT   1   14-DEC-16 5EYO    0                                                
JRNL        AUTH   D.WANG,H.HASHIMOTO,X.ZHANG,B.G.BARWICK,S.LONIAL,L.H.BOISE,   
JRNL        AUTH 2 P.M.VERTINO,X.CHENG                                          
JRNL        TITL   MAX IS AN EPIGENETIC SENSOR OF 5-CARBOXYLCYTOSINE AND IS     
JRNL        TITL 2 ALTERED IN MULTIPLE MYELOMA.                                 
JRNL        REF    NUCLEIC ACIDS RES.            V.  45  2396 2017              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   27903915                                                     
JRNL        DOI    10.1093/NAR/GKW1184                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.39 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.11_2558: ???)                              
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.39                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 38.27                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.380                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.9                           
REMARK   3   NUMBER OF REFLECTIONS             : 18257                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.205                           
REMARK   3   R VALUE            (WORKING SET) : 0.203                           
REMARK   3   FREE R VALUE                     : 0.229                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.970                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 908                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 38.2749 -  4.3413    1.00     2935   155  0.1670 0.1961        
REMARK   3     2  4.3413 -  3.4466    1.00     2920   153  0.1861 0.1980        
REMARK   3     3  3.4466 -  3.0111    0.98     2843   149  0.2333 0.2573        
REMARK   3     4  3.0111 -  2.7359    1.00     2879   150  0.2656 0.3368        
REMARK   3     5  2.7359 -  2.5398    0.98     2910   153  0.2899 0.3242        
REMARK   3     6  2.5398 -  2.3901    0.98     2862   148  0.3421 0.3753        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.330            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 33.490           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 60.34                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.003           2155                                  
REMARK   3   ANGLE     :  0.546           3041                                  
REMARK   3   CHIRALITY :  0.030            331                                  
REMARK   3   PLANARITY :  0.002            290                                  
REMARK   3   DIHEDRAL  : 20.539           1207                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 16                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: (CHAIN A AND RESID 20:33)                              
REMARK   3    ORIGIN FOR THE GROUP (A):  -2.4621  11.3504  11.5523              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.7748 T22:   0.7517                                     
REMARK   3      T33:   0.6450 T12:   0.0940                                     
REMARK   3      T13:  -0.0036 T23:  -0.1000                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.6000 L22:   4.3166                                     
REMARK   3      L33:   3.5494 L12:   4.3821                                     
REMARK   3      L13:   2.5409 L23:   2.9715                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.5191 S12:   0.7678 S13:  -0.7215                       
REMARK   3      S21:  -1.0867 S22:   0.8605 S23:  -0.6968                       
REMARK   3      S31:   1.7655 S32:  -0.6619 S33:  -0.3552                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: (CHAIN A AND RESID 34:56)                              
REMARK   3    ORIGIN FOR THE GROUP (A):   1.7578  33.7467  22.3327              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.0410 T22:   0.3837                                     
REMARK   3      T33:   0.8466 T12:   0.0011                                     
REMARK   3      T13:  -0.1986 T23:  -0.0533                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.7431 L22:   4.2723                                     
REMARK   3      L33:   7.8617 L12:  -3.4739                                     
REMARK   3      L13:  -4.7806 L23:   5.7839                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1511 S12:  -0.0244 S13:   0.7489                       
REMARK   3      S21:  -0.2344 S22:   0.4793 S23:  -2.3691                       
REMARK   3      S31:   0.1723 S32:   1.1411 S33:  -0.6308                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: (CHAIN A AND RESID 57:73)                              
REMARK   3    ORIGIN FOR THE GROUP (A):  -4.9272  39.6286  29.9431              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.1823 T22:   0.3410                                     
REMARK   3      T33:   0.7718 T12:   0.0499                                     
REMARK   3      T13:  -0.1873 T23:  -0.0002                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.6756 L22:   2.2747                                     
REMARK   3      L33:   3.3550 L12:  -2.6680                                     
REMARK   3      L13:  -3.5591 L23:   2.7353                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2124 S12:  -0.7528 S13:   0.7705                       
REMARK   3      S21:   1.1360 S22:   0.6061 S23:  -0.3743                       
REMARK   3      S31:   0.0392 S32:   0.2282 S33:  -0.7572                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: (CHAIN A AND RESID 74:93)                              
REMARK   3    ORIGIN FOR THE GROUP (A): -10.2944  63.6846  24.8305              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3557 T22:   0.4645                                     
REMARK   3      T33:   1.4910 T12:  -0.0436                                     
REMARK   3      T13:   0.2105 T23:   0.0593                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.7328 L22:   5.4476                                     
REMARK   3      L33:   6.6991 L12:  -1.9443                                     
REMARK   3      L13:  -0.2917 L23:  -0.6774                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0828 S12:  -0.2391 S13:   0.9231                       
REMARK   3      S21:   0.2155 S22:  -0.0736 S23:   0.4003                       
REMARK   3      S31:   0.6216 S32:  -0.0196 S33:   0.2491                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: (CHAIN A AND RESID 94:105)                             
REMARK   3    ORIGIN FOR THE GROUP (A):  -8.7521  87.2996  20.1058              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7073 T22:   0.3693                                     
REMARK   3      T33:   2.4501 T12:  -0.0355                                     
REMARK   3      T13:   0.2386 T23:   0.1026                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.5315 L22:   8.4833                                     
REMARK   3      L33:   8.7714 L12:   2.3751                                     
REMARK   3      L13:   1.8303 L23:  -2.7794                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1004 S12:   0.4235 S13:   0.7220                       
REMARK   3      S21:  -1.1728 S22:  -0.4357 S23:  -1.0381                       
REMARK   3      S31:  -0.6847 S32:   0.5434 S33:   0.3807                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: (CHAIN B AND RESID 4:7)                                
REMARK   3    ORIGIN FOR THE GROUP (A):  -1.5374  17.8746  42.9701              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.8618 T22:   1.0490                                     
REMARK   3      T33:   0.4786 T12:  -0.1292                                     
REMARK   3      T13:  -0.4105 T23:  -0.0035                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.5750 L22:   0.1603                                     
REMARK   3      L33:   2.0018 L12:  -1.0946                                     
REMARK   3      L13:   8.6657 L23:  -1.2200                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.6004 S12:  -1.4351 S13:  -0.7308                       
REMARK   3      S21:   1.4422 S22:   0.4866 S23:   0.5408                       
REMARK   3      S31:   0.9673 S32:  -0.7029 S33:   0.1311                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: (CHAIN B AND RESID 8:15)                               
REMARK   3    ORIGIN FOR THE GROUP (A):  -4.1935  16.6014  22.7616              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.6784 T22:   0.5897                                     
REMARK   3      T33:   0.4907 T12:   0.0268                                     
REMARK   3      T13:  -0.5031 T23:   0.0065                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.4344 L22:   1.5967                                     
REMARK   3      L33:   6.9711 L12:   0.7368                                     
REMARK   3      L13:   2.3000 L23:   3.2587                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3937 S12:   0.2943 S13:  -0.3752                       
REMARK   3      S21:  -0.4363 S22:   0.2222 S23:  -0.2918                       
REMARK   3      S31:  -0.2968 S32:  -0.2547 S33:  -0.2492                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: (CHAIN B AND RESID 16:19)                              
REMARK   3    ORIGIN FOR THE GROUP (A): -17.9936  21.0226   8.0098              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.8359 T22:   1.1031                                     
REMARK   3      T33:   0.5783 T12:   0.2322                                     
REMARK   3      T13:  -0.3589 T23:   0.0391                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.7030 L22:   1.8652                                     
REMARK   3      L33:   7.8068 L12:   1.4898                                     
REMARK   3      L13:  -6.0881 L23:  -0.8807                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.8929 S12:   0.5433 S13:   0.5177                       
REMARK   3      S21:   1.3742 S22:  -0.6434 S23:   0.0883                       
REMARK   3      S31:  -3.3752 S32:  -0.3043 S33:   0.7009                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: (CHAIN C AND RESID 20:25)                              
REMARK   3    ORIGIN FOR THE GROUP (A):   5.5390   2.8591  31.2326              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.9020 T22:   0.6460                                     
REMARK   3      T33:   1.2245 T12:  -0.2155                                     
REMARK   3      T13:  -0.2191 T23:  -0.0100                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.9688 L22:   8.4865                                     
REMARK   3      L33:   5.2790 L12:   6.5437                                     
REMARK   3      L13:   5.4279 L23:   5.2823                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -1.5483 S12:  -0.4450 S13:   2.8965                       
REMARK   3      S21:  -0.3458 S22:  -0.0058 S23:  -1.2375                       
REMARK   3      S31:  -2.0293 S32:   0.5240 S33:   1.4725                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: (CHAIN C AND RESID 26:52)                              
REMARK   3    ORIGIN FOR THE GROUP (A):   2.4092 -20.1260  23.0095              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.2251 T22:   0.4709                                     
REMARK   3      T33:   0.8121 T12:  -0.1828                                     
REMARK   3      T13:   0.1239 T23:  -0.1137                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.7105 L22:   6.7510                                     
REMARK   3      L33:   2.0110 L12:   2.9963                                     
REMARK   3      L13:   2.0683 L23:  -1.3308                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.5684 S12:   0.4516 S13:  -1.0436                       
REMARK   3      S21:  -0.7940 S22:   0.4305 S23:  -0.6354                       
REMARK   3      S31:  -0.6118 S32:   0.9180 S33:   0.1460                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: (CHAIN C AND RESID 53:78)                              
REMARK   3    ORIGIN FOR THE GROUP (A):  -8.8347 -34.3406  18.9185              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.1909 T22:   0.4378                                     
REMARK   3      T33:   1.3203 T12:   0.0567                                     
REMARK   3      T13:  -0.0643 T23:  -0.1836                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.7599 L22:   5.6654                                     
REMARK   3      L33:   3.8686 L12:  -1.5757                                     
REMARK   3      L13:   1.6016 L23:  -2.1804                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0856 S12:   0.5989 S13:  -1.6327                       
REMARK   3      S21:  -1.8584 S22:   0.4233 S23:  -0.5489                       
REMARK   3      S31:   0.5455 S32:  -0.1173 S33:  -0.0099                       
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    SELECTION: (CHAIN C AND RESID 79:100)                             
REMARK   3    ORIGIN FOR THE GROUP (A):  -8.3403 -65.4973  27.4943              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6783 T22:   0.4611                                     
REMARK   3      T33:   1.6873 T12:  -0.0880                                     
REMARK   3      T13:  -0.0432 T23:   0.0094                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.0874 L22:   2.1283                                     
REMARK   3      L33:   5.2147 L12:  -1.1409                                     
REMARK   3      L13:   0.4614 L23:  -3.1270                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0964 S12:  -0.1128 S13:   0.3785                       
REMARK   3      S21:   1.2068 S22:  -0.4235 S23:  -0.3260                       
REMARK   3      S31:  -0.7543 S32:   0.0281 S33:   0.3568                       
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    SELECTION: (CHAIN C AND RESID 101:107)                            
REMARK   3    ORIGIN FOR THE GROUP (A):  -4.1670 -87.2552  28.1596              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5954 T22:   0.3192                                     
REMARK   3      T33:   1.9988 T12:  -0.0338                                     
REMARK   3      T13:   0.0612 T23:   0.0660                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.6882 L22:   3.0636                                     
REMARK   3      L33:   9.0042 L12:  -1.4524                                     
REMARK   3      L13:  -2.4874 L23:   5.2513                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.4008 S12:  -0.3474 S13:  -0.1571                       
REMARK   3      S21:   1.3185 S22:  -0.2109 S23:  -0.6319                       
REMARK   3      S31:   1.0729 S32:   0.3920 S33:   0.4985                       
REMARK   3   TLS GROUP : 14                                                     
REMARK   3    SELECTION: (CHAIN D AND RESID 4:7)                                
REMARK   3    ORIGIN FOR THE GROUP (A): -16.9369  -9.9420   7.5941              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.7501 T22:   0.9067                                     
REMARK   3      T33:   0.5659 T12:   0.1028                                     
REMARK   3      T13:  -0.2660 T23:  -0.1230                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.5412 L22:   8.3613                                     
REMARK   3      L33:   7.9281 L12:   0.0451                                     
REMARK   3      L13:  -1.4091 L23:  -3.1970                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1652 S12:   2.1916 S13:  -0.6675                       
REMARK   3      S21:   0.2088 S22:   0.1974 S23:   0.9938                       
REMARK   3      S31:  -1.7102 S32:  -0.1344 S33:  -0.2274                       
REMARK   3   TLS GROUP : 15                                                     
REMARK   3    SELECTION: (CHAIN D AND RESID 8:13)                               
REMARK   3    ORIGIN FOR THE GROUP (A):  -3.5341 -10.3245  19.7056              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.3878 T22:   0.4939                                     
REMARK   3      T33:   0.4423 T12:  -0.0907                                     
REMARK   3      T13:  -0.0717 T23:   0.0162                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.8112 L22:   7.8572                                     
REMARK   3      L33:   7.5663 L12:   6.1745                                     
REMARK   3      L13:   5.9255 L23:   7.5192                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2586 S12:   0.0847 S13:  -0.4695                       
REMARK   3      S21:   0.2552 S22:   0.1633 S23:  -0.4885                       
REMARK   3      S31:  -0.8417 S32:   0.1831 S33:  -0.4448                       
REMARK   3   TLS GROUP : 16                                                     
REMARK   3    SELECTION: (CHAIN D AND RESID 14:19)                              
REMARK   3    ORIGIN FOR THE GROUP (A):  -3.4435 -10.1289  40.4794              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.7136 T22:   0.9792                                     
REMARK   3      T33:   0.4544 T12:   0.0232                                     
REMARK   3      T13:  -0.0491 T23:   0.0592                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.8826 L22:   4.4615                                     
REMARK   3      L33:   3.0545 L12:  -0.5054                                     
REMARK   3      L13:   1.4649 L23:  -3.5693                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0281 S12:  -1.2346 S13:  -0.7682                       
REMARK   3      S21:   0.0964 S22:   0.3891 S23:   0.0683                       
REMARK   3      S31:  -1.5389 S32:  -0.2966 S33:  -0.4401                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5EYO COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 25-NOV-15.                  
REMARK 100 THE DEPOSITION ID IS D_1000215705.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 20-AUG-15                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.6                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 22-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.0000                             
REMARK 200  MONOCHROMATOR                  : SI(111)                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : RAYONIX MX300-HS                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XDS                                
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 18442                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.390                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 38.270                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.7                               
REMARK 200  DATA REDUNDANCY                : 7.600                              
REMARK 200  R MERGE                    (I) : 0.06700                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 15.7000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.39                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.45                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.30                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.95300                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.000                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHENIX                                                
REMARK 200 STARTING MODEL: 1AN2                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 68.21                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.87                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM     
REMARK 280  CITRATE-HCL PH 5.6, 25% (W/V) PEG 4000, VAPOR DIFFUSION, SITTING    
REMARK 280  DROP, TEMPERATURE 289K                                              
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       16.37500            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000      153.08000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       16.37500            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000      153.08000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7780 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 15240 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -60.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000      -11.69414            
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000       47.64589            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7490 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 15290 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -68.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000      -11.69414            
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000       47.64589            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER A   107                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LYS A  24    CG   CD   CE   NZ                                   
REMARK 470     GLN A  54    CG   CD   OE1  NE2                                  
REMARK 470     GLU A  56    CG   CD   OE1  OE2                                  
REMARK 470     LYS A  57    CG   CD   CE   NZ                                   
REMARK 470     LYS A 104    CG   CD   CE   NZ                                   
REMARK 470     ARG A 106    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS C  24    CG   CD   CE   NZ                                   
REMARK 470     GLN C  54    CG   CD   OE1  NE2                                  
REMARK 470     GLU C  56    CG   CD   OE1  OE2                                  
REMARK 470     LYS C  57    CG   CD   CE   NZ                                   
REMARK 470     GLN C  72    CG   CD   OE1  NE2                                  
REMARK 470     MET C  74    CE                                                  
REMARK 470     ARG C  75    NE   CZ   NH1  NH2                                  
REMARK 470     ARG C  76    CD   NE   CZ   NH1  NH2                             
REMARK 470     LYS C  77    NZ                                                  
REMARK 470     GLN C  83    CG   CD   OE1  NE2                                  
REMARK 470     ASP C  86    CG   OD1  OD2                                       
REMARK 470     LYS C  89    CD   CE   NZ                                        
REMARK 470     ARG C  90    CD   NE   CZ   NH1  NH2                             
REMARK 470     GLN C  97    OE1  NE2                                            
REMARK 470     LYS C 104    CE   NZ                                             
DBREF  5EYO A   22   107  UNP    P61244   MAX_HUMAN       22    107             
DBREF  5EYO B    4    19  PDB    5EYO     5EYO             4     19             
DBREF  5EYO C   22   107  UNP    P61244   MAX_HUMAN       22    107             
DBREF  5EYO D    4    19  PDB    5EYO     5EYO             4     19             
SEQADV 5EYO HIS A   20  UNP  P61244              EXPRESSION TAG                 
SEQADV 5EYO MET A   21  UNP  P61244              EXPRESSION TAG                 
SEQADV 5EYO HIS C   20  UNP  P61244              EXPRESSION TAG                 
SEQADV 5EYO MET C   21  UNP  P61244              EXPRESSION TAG                 
SEQRES   1 A   88  HIS MET ALA ASP LYS ARG ALA HIS HIS ASN ALA LEU GLU          
SEQRES   2 A   88  ARG LYS ARG ARG ASP HIS ILE LYS ASP SER PHE HIS SER          
SEQRES   3 A   88  LEU ARG ASP SER VAL PRO SER LEU GLN GLY GLU LYS ALA          
SEQRES   4 A   88  SER ARG ALA GLN ILE LEU ASP LYS ALA THR GLU TYR ILE          
SEQRES   5 A   88  GLN TYR MET ARG ARG LYS ASN HIS THR HIS GLN GLN ASP          
SEQRES   6 A   88  ILE ASP ASP LEU LYS ARG GLN ASN ALA LEU LEU GLU GLN          
SEQRES   7 A   88  GLN VAL ARG ALA LEU GLU LYS ALA ARG SER                      
SEQRES   1 B   16   DA  DG  DT  DA  DG  DC  DA 1CC  DG  DT  DG  DC  DT          
SEQRES   2 B   16   DA  DC  DT                                                  
SEQRES   1 C   88  HIS MET ALA ASP LYS ARG ALA HIS HIS ASN ALA LEU GLU          
SEQRES   2 C   88  ARG LYS ARG ARG ASP HIS ILE LYS ASP SER PHE HIS SER          
SEQRES   3 C   88  LEU ARG ASP SER VAL PRO SER LEU GLN GLY GLU LYS ALA          
SEQRES   4 C   88  SER ARG ALA GLN ILE LEU ASP LYS ALA THR GLU TYR ILE          
SEQRES   5 C   88  GLN TYR MET ARG ARG LYS ASN HIS THR HIS GLN GLN ASP          
SEQRES   6 C   88  ILE ASP ASP LEU LYS ARG GLN ASN ALA LEU LEU GLU GLN          
SEQRES   7 C   88  GLN VAL ARG ALA LEU GLU LYS ALA ARG SER                      
SEQRES   1 D   16   DA  DG  DT  DA  DG  DC  DA 1CC  DG  DT  DG  DC  DT          
SEQRES   2 D   16   DA  DC  DT                                                  
HET    1CC  B  11      22                                                       
HET    1CC  D  11      22                                                       
HETNAM     1CC 5-CARBOXY-2'-DEOXYCYTIDINE MONOPHOSPHATE                         
FORMUL   2  1CC    2(C10 H14 N3 O9 P)                                           
FORMUL   5  HOH   *11(H2 O)                                                     
HELIX    1 AA1 HIS A   20  ASP A   48  1                                  29    
HELIX    2 AA2 SER A   59  GLU A  103  1                                  45    
HELIX    3 AA3 MET C   21  VAL C   50  1                                  30    
HELIX    4 AA4 SER C   59  ALA C  105  1                                  47    
LINK         O3'  DA B  10                 P   1CC B  11     1555   1555  1.61  
LINK         O3' 1CC B  11                 P    DG B  12     1555   1555  1.61  
LINK         O3'  DA D  10                 P   1CC D  11     1555   1555  1.61  
LINK         O3' 1CC D  11                 P    DG D  12     1555   1555  1.61  
CRYST1   32.750  306.160   49.060  90.00 103.79  90.00 C 1 2 1       8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.030534  0.000000  0.007494        0.00000                         
SCALE2      0.000000  0.003266  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.020988        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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