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Database: PDB
Entry: 5JD0
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HEADER    SIGNALING PROTEIN                       15-APR-16   5JD0              
TITLE     CRYSTAL STRUCTURE OF ARAP3 RHOGAP DOMAIN                              
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: ARF-GAP WITH RHO-GAP DOMAIN, ANK REPEAT AND PH DOMAIN-     
COMPND   3 CONTAINING PROTEIN 3;                                                
COMPND   4 CHAIN: A, B;                                                         
COMPND   5 FRAGMENT: UNP RESIDUES 906-1107;                                     
COMPND   6 SYNONYM: CENTAURIN-DELTA-3,CNT-D3;                                   
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ARAP3;                                                         
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI 'BL21-GOLD(DE3)PLYSS AG';        
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 866768;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21-GOLD(DE3)PLYSS AG                     
KEYWDS    ARAP3, RHOGAP, SIGNALING PROTEIN                                      
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    H.BAO,F.LI,J.WU,Y.SHI                                                 
REVDAT   2   17-AUG-16 5JD0    1       JRNL                                     
REVDAT   1   22-JUN-16 5JD0    0                                                
JRNL        AUTH   H.BAO,F.LI,C.WANG,N.WANG,Y.JIANG,Y.TANG,J.WU,Y.SHI           
JRNL        TITL   STRUCTURAL BASIS FOR THE SPECIFIC RECOGNITION OF RHOA BY THE 
JRNL        TITL 2 DUAL GTPASE-ACTIVATING PROTEIN ARAP3                         
JRNL        REF    J.BIOL.CHEM.                  V. 291 16709 2016              
JRNL        REFN                   ESSN 1083-351X                               
JRNL        PMID   27311713                                                     
JRNL        DOI    10.1074/JBC.M116.736140                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.30 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.10_2155: ???)                              
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.30                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 38.36                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.1                           
REMARK   3   NUMBER OF REFLECTIONS             : 25555                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.222                           
REMARK   3   R VALUE            (WORKING SET) : 0.220                           
REMARK   3   FREE R VALUE                     : 0.260                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.090                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1301                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 38.3694 -  4.7816    1.00     2886   167  0.2102 0.2187        
REMARK   3     2  4.7816 -  3.7964    1.00     2761   147  0.1918 0.2384        
REMARK   3     3  3.7964 -  3.3168    0.92     2518   133  0.2335 0.2717        
REMARK   3     4  3.3168 -  3.0137    1.00     2704   138  0.2421 0.2777        
REMARK   3     5  3.0137 -  2.7977    1.00     2685   155  0.2450 0.3039        
REMARK   3     6  2.7977 -  2.6328    1.00     2679   141  0.2178 0.2709        
REMARK   3     7  2.6328 -  2.5010    1.00     2665   157  0.2229 0.2859        
REMARK   3     8  2.5010 -  2.3921    1.00     2671   148  0.2318 0.2951        
REMARK   3     9  2.3921 -  2.3001    1.00     2685   115  0.2490 0.3430        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.280            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 26.690           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 36.17                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.008           3135                                  
REMARK   3   ANGLE     :  0.916           4250                                  
REMARK   3   CHIRALITY :  0.052            500                                  
REMARK   3   PLANARITY :  0.006            555                                  
REMARK   3   DIHEDRAL  : 18.263           1906                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 1                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: ALL                                                    
REMARK   3    ORIGIN FOR THE GROUP (A):  -9.8014 -39.1162  11.9869              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2057 T22:   0.1564                                     
REMARK   3      T33:   0.2862 T12:   0.0438                                     
REMARK   3      T13:  -0.0466 T23:  -0.0209                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.0313 L22:   0.9750                                     
REMARK   3      L33:   1.3472 L12:   0.4299                                     
REMARK   3      L13:  -0.5279 L23:   0.4514                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0840 S12:  -0.0079 S13:  -0.0635                       
REMARK   3      S21:   0.0467 S22:   0.0682 S23:  -0.0497                       
REMARK   3      S31:   0.0800 S32:  -0.0020 S33:   0.0257                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5JD0 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 19-APR-16.                  
REMARK 100 THE DEPOSITION ID IS D_1000220356.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 23-DEC-12                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.9                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SSRF                               
REMARK 200  BEAMLINE                       : BL17U                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97931                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315R                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : SCALA                              
REMARK 200  DATA SCALING SOFTWARE          : SCALA 3.3.22                       
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 25608                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.300                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 76.728                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.1                               
REMARK 200  DATA REDUNDANCY                : 9.100                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : 0.13800                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 11.1000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.30                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.42                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 9.40                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.64200                            
REMARK 200  R SYM FOR SHELL            (I) : 0.64200                            
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.200                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 2EE5                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 59.59                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.04                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 100 MM HEPES, 10% PEG8000, PH 6.9,       
REMARK 280  VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 43 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -Y+1/2,X+1/2,Z+3/4                                      
REMARK 290       4555   Y+1/2,-X+1/2,Z+1/4                                      
REMARK 290       5555   -X+1/2,Y+1/2,-Z+3/4                                     
REMARK 290       6555   X+1/2,-Y+1/2,-Z+1/4                                     
REMARK 290       7555   Y,X,-Z                                                  
REMARK 290       8555   -Y,-X,-Z+1/2                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       47.50500            
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       54.25500            
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       54.25500            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       71.25750            
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       54.25500            
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       54.25500            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       23.75250            
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       54.25500            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       54.25500            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       71.25750            
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       54.25500            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       54.25500            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       23.75250            
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       47.50500            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A   897                                                      
REMARK 465     GLY A   898                                                      
REMARK 465     HIS A   899                                                      
REMARK 465     HIS A   900                                                      
REMARK 465     HIS A   901                                                      
REMARK 465     HIS A   902                                                      
REMARK 465     HIS A   903                                                      
REMARK 465     HIS A   904                                                      
REMARK 465     MET A   905                                                      
REMARK 465     GLY A   906                                                      
REMARK 465     MET B   897                                                      
REMARK 465     GLY B   898                                                      
REMARK 465     HIS B   899                                                      
REMARK 465     HIS B   900                                                      
REMARK 465     HIS B   901                                                      
REMARK 465     HIS B   902                                                      
REMARK 465     HIS B   903                                                      
REMARK 465     HIS B   904                                                      
REMARK 465     MET B   905                                                      
REMARK 465     THR B  1107                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLN A 910    CG   CD   OE1  NE2                                  
REMARK 470     GLU A 911    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 943    CG   CD   CE   NZ                                   
REMARK 470     ARG A 959    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG A 967    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 970    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 985    NE   CZ   NH1  NH2                                  
REMARK 470     ASP A 988    CG   OD1  OD2                                       
REMARK 470     GLU A1005    CD   OE1  OE2                                       
REMARK 470     GLN A1008    CG   CD   OE1  NE2                                  
REMARK 470     LYS A1009    CE   NZ                                             
REMARK 470     ARG A1025    NE   CZ   NH1                                       
REMARK 470     ASP A1067    CG   OD1  OD2                                       
REMARK 470     ARG A1069    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A1071    CG   CD   OE1  OE2                                  
REMARK 470     ARG B 916    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG B 936    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU B 938    CG   CD   OE1  OE2                                  
REMARK 470     GLU B 956    CG   CD   OE1  OE2                                  
REMARK 470     ARG B 959    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS B 965    CG   CD   CE   NZ                                   
REMARK 470     GLU B 970    CD   OE1  OE2                                       
REMARK 470     ARG B 995    CD   NE   CZ   NH1  NH2                             
REMARK 470     LYS B1009    CG   CD   CE   NZ                                   
REMARK 470     LYS B1017    CG   CD   CE   NZ                                   
REMARK 470     ARG B1029    NE   CZ   NH1  NH2                                  
REMARK 470     GLN B1049    CG   CD   OE1  NE2                                  
REMARK 470     ARG B1069    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU B1071    CG   CD   OE1  OE2                                  
REMARK 470     SER B1092    OG                                                  
REMARK 470     GLN B1097    CG   CD   OE1  NE2                                  
REMARK 470     SER B1103    OG                                                  
REMARK 470     LEU B1104    CG   CD1  CD2                                       
REMARK 470     THR B1106    OG1  CG2                                            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   NH1  ARG B  1040     OE1  GLU B  1101              2.15            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS A 943       93.41    -69.51                                   
REMARK 500    ASP A 960       91.12   -164.65                                   
REMARK 500    TYR B 941      -16.39     73.11                                   
REMARK 500    ASP B 960       89.77   -164.36                                   
REMARK 500    ASP B1067       55.22   -106.35                                   
REMARK 500    ARG B1069      103.79   -161.49                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525                                                                      
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT                    
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST                  
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT                 
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE                       
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;                             
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE                  
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH A1246        DISTANCE =  7.27 ANGSTROMS                       
REMARK 525    HOH A1247        DISTANCE =  7.29 ANGSTROMS                       
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5JCP   RELATED DB: PDB                                   
DBREF  5JD0 A  906  1107  UNP    Q8WWN8   ARAP3_HUMAN    906   1107             
DBREF  5JD0 B  906  1107  UNP    Q8WWN8   ARAP3_HUMAN    906   1107             
SEQADV 5JD0 MET A  897  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 GLY A  898  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS A  899  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS A  900  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS A  901  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS A  902  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS A  903  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS A  904  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 MET A  905  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 MET B  897  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 GLY B  898  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS B  899  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS B  900  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS B  901  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS B  902  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS B  903  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 HIS B  904  UNP  Q8WWN8              EXPRESSION TAG                 
SEQADV 5JD0 MET B  905  UNP  Q8WWN8              EXPRESSION TAG                 
SEQRES   1 A  211  MET GLY HIS HIS HIS HIS HIS HIS MET GLY THR GLY LEU          
SEQRES   2 A  211  GLN GLU GLN GLN MET SER ARG GLY ASP ILE PRO ILE ILE          
SEQRES   3 A  211  VAL ASP ALA CYS ILE SER PHE VAL THR GLN HIS GLY LEU          
SEQRES   4 A  211  ARG LEU GLU GLY VAL TYR ARG LYS GLY GLY ALA ARG ALA          
SEQRES   5 A  211  ARG SER LEU ARG LEU LEU ALA GLU PHE ARG ARG ASP ALA          
SEQRES   6 A  211  ARG SER VAL LYS LEU ARG PRO GLY GLU HIS PHE VAL GLU          
SEQRES   7 A  211  ASP VAL THR ASP THR LEU LYS ARG PHE PHE ARG GLU LEU          
SEQRES   8 A  211  ASP ASP PRO VAL THR SER ALA ARG LEU LEU PRO ARG TRP          
SEQRES   9 A  211  ARG GLU ALA ALA GLU LEU PRO GLN LYS ASN GLN ARG LEU          
SEQRES  10 A  211  GLU LYS TYR LYS ASP VAL ILE GLY CYS LEU PRO ARG VAL          
SEQRES  11 A  211  ASN ARG ARG THR LEU ALA THR LEU ILE GLY HIS LEU TYR          
SEQRES  12 A  211  ARG VAL GLN LYS CYS ALA ALA LEU ASN GLN MET CYS THR          
SEQRES  13 A  211  ARG ASN LEU ALA LEU LEU PHE ALA PRO SER VAL PHE GLN          
SEQRES  14 A  211  THR ASP GLY ARG GLY GLU HIS GLU VAL ARG VAL LEU GLN          
SEQRES  15 A  211  GLU LEU ILE ASP GLY TYR ILE SER VAL PHE ASP ILE ASP          
SEQRES  16 A  211  SER ASP GLN VAL ALA GLN ILE ASP LEU GLU VAL SER LEU          
SEQRES  17 A  211  ILE THR THR                                                  
SEQRES   1 B  211  MET GLY HIS HIS HIS HIS HIS HIS MET GLY THR GLY LEU          
SEQRES   2 B  211  GLN GLU GLN GLN MET SER ARG GLY ASP ILE PRO ILE ILE          
SEQRES   3 B  211  VAL ASP ALA CYS ILE SER PHE VAL THR GLN HIS GLY LEU          
SEQRES   4 B  211  ARG LEU GLU GLY VAL TYR ARG LYS GLY GLY ALA ARG ALA          
SEQRES   5 B  211  ARG SER LEU ARG LEU LEU ALA GLU PHE ARG ARG ASP ALA          
SEQRES   6 B  211  ARG SER VAL LYS LEU ARG PRO GLY GLU HIS PHE VAL GLU          
SEQRES   7 B  211  ASP VAL THR ASP THR LEU LYS ARG PHE PHE ARG GLU LEU          
SEQRES   8 B  211  ASP ASP PRO VAL THR SER ALA ARG LEU LEU PRO ARG TRP          
SEQRES   9 B  211  ARG GLU ALA ALA GLU LEU PRO GLN LYS ASN GLN ARG LEU          
SEQRES  10 B  211  GLU LYS TYR LYS ASP VAL ILE GLY CYS LEU PRO ARG VAL          
SEQRES  11 B  211  ASN ARG ARG THR LEU ALA THR LEU ILE GLY HIS LEU TYR          
SEQRES  12 B  211  ARG VAL GLN LYS CYS ALA ALA LEU ASN GLN MET CYS THR          
SEQRES  13 B  211  ARG ASN LEU ALA LEU LEU PHE ALA PRO SER VAL PHE GLN          
SEQRES  14 B  211  THR ASP GLY ARG GLY GLU HIS GLU VAL ARG VAL LEU GLN          
SEQRES  15 B  211  GLU LEU ILE ASP GLY TYR ILE SER VAL PHE ASP ILE ASP          
SEQRES  16 B  211  SER ASP GLN VAL ALA GLN ILE ASP LEU GLU VAL SER LEU          
SEQRES  17 B  211  ILE THR THR                                                  
FORMUL   3  HOH   *89(H2 O)                                                     
HELIX    1 AA1 GLY A  908  GLN A  912  5                                   5    
HELIX    2 AA2 PRO A  920  GLY A  934  1                                  15    
HELIX    3 AA3 ALA A  946  ALA A  961  1                                  16    
HELIX    4 AA4 ARG A  962  VAL A  964  5                                   3    
HELIX    5 AA5 PHE A  972  LEU A  987  1                                  16    
HELIX    6 AA6 LEU A  996  GLU A 1005  1                                  10    
HELIX    7 AA7 GLN A 1008  GLY A 1021  1                                  14    
HELIX    8 AA8 PRO A 1024  CYS A 1044  1                                  21    
HELIX    9 AA9 CYS A 1044  GLN A 1049  1                                   6    
HELIX   10 AB1 CYS A 1051  GLN A 1065  1                                  15    
HELIX   11 AB2 GLY A 1070  GLY A 1083  1                                  14    
HELIX   12 AB3 GLY A 1083  PHE A 1088  1                                   6    
HELIX   13 AB4 ASP A 1091  THR A 1107  1                                  17    
HELIX   14 AB5 GLY B  908  GLN B  912  5                                   5    
HELIX   15 AB6 PRO B  920  GLY B  934  1                                  15    
HELIX   16 AB7 ALA B  946  ALA B  961  1                                  16    
HELIX   17 AB8 ARG B  962  VAL B  964  5                                   3    
HELIX   18 AB9 PHE B  972  LEU B  987  1                                  16    
HELIX   19 AC1 LEU B  996  GLU B 1005  1                                  10    
HELIX   20 AC2 GLN B 1008  GLY B 1021  1                                  14    
HELIX   21 AC3 PRO B 1024  CYS B 1044  1                                  21    
HELIX   22 AC4 CYS B 1044  GLN B 1049  1                                   6    
HELIX   23 AC5 CYS B 1051  PHE B 1064  1                                  14    
HELIX   24 AC6 GLY B 1070  GLY B 1083  1                                  14    
HELIX   25 AC7 GLY B 1083  PHE B 1088  1                                   6    
HELIX   26 AC8 ASP B 1091  ILE B 1105  1                                  15    
CISPEP   1 ARG B 1069    GLY B 1070          0        -2.69                     
CRYST1  108.510  108.510   95.010  90.00  90.00  90.00 P 43 21 2    16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009216  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.009216  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.010525        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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