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Database: PDB
Entry: 5M2C
LinkDB: 5M2C
Original site: 5M2C 
HEADER    CELL ADHESION                           12-OCT-16   5M2C              
TITLE     STRUCTURAL TUNING OF CD81LEL (SPACE GROUP P32 1 2)                    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CD81 ANTIGEN;                                              
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: 26 KDA CELL SURFACE PROTEIN TAPA-1,TARGET OF THE            
COMPND   5 ANTIPROLIFERATIVE ANTIBODY 1,TETRASPANIN-28,TSPAN-28;                
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 OTHER_DETAILS: LARGE-EXTRACELLULAR-LOOP                              
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: CD81, TAPA1, TSPAN28;                                          
SOURCE   6 EXPRESSION_SYSTEM: HOMO SAPIENS;                                     
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 9606;                                       
SOURCE   8 EXPRESSION_SYSTEM_CELL_LINE: HEK293T;                                
SOURCE   9 EXPRESSION_SYSTEM_CELL: HUMAN EMBRYO KIDNEY;                         
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PHLSEC                                    
KEYWDS    HUMAN CELLULAR RECEPTOR FOR HEPATITIS C VIRUS, CELL ADHESION          
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    E.S.CUNHA,P.SFRISO,A.L.ROJAS,P.ROVERSI,A.HOSPITAL,M.OROZCO,           
AUTHOR   2 N.G.ABRESCIA                                                         
REVDAT   4   31-JAN-18 5M2C    1       REMARK                                   
REVDAT   3   13-DEC-17 5M2C    1       AUTHOR JRNL                              
REVDAT   2   18-JAN-17 5M2C    1       JRNL                                     
REVDAT   1   14-DEC-16 5M2C    0                                                
JRNL        AUTH   E.S.CUNHA,P.SFRISO,A.L.ROJAS,P.ROVERSI,A.HOSPITAL,M.OROZCO,  
JRNL        AUTH 2 N.G.ABRESCIA                                                 
JRNL        TITL   MECHANISM OF STRUCTURAL TUNING OF THE HEPATITIS C VIRUS      
JRNL        TITL 2 HUMAN CELLULAR RECEPTOR CD81 LARGE EXTRACELLULAR LOOP.       
JRNL        REF    STRUCTURE                     V.  25    53 2017              
JRNL        REFN                   ISSN 1878-4186                               
JRNL        PMID   27916518                                                     
JRNL        DOI    10.1016/J.STR.2016.11.003                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.96 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.9_1692                                      
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.96                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 41.07                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.370                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.9                           
REMARK   3   NUMBER OF REFLECTIONS             : 13870                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.206                           
REMARK   3   R VALUE            (WORKING SET) : 0.204                           
REMARK   3   FREE R VALUE                     : 0.234                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.310                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 737                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 41.0763 -  3.3528    1.00     2732   129  0.1936 0.2367        
REMARK   3     2  3.3528 -  2.6613    1.00     2627   151  0.2205 0.2239        
REMARK   3     3  2.6613 -  2.3250    1.00     2608   141  0.2055 0.2195        
REMARK   3     4  2.3250 -  2.1124    1.00     2580   160  0.2086 0.2689        
REMARK   3     5  2.1124 -  1.9610    1.00     2586   156  0.2269 0.2292        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 0.90                                          
REMARK   3   SHRINKAGE RADIUS   : 0.50                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.190            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 24.600           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.006           1370                                  
REMARK   3   ANGLE     :  0.918           1846                                  
REMARK   3   CHIRALITY :  0.033            220                                  
REMARK   3   PLANARITY :  0.003            240                                  
REMARK   3   DIHEDRAL  : 15.017            494                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 4                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN A AND (RESID 114:157 OR RESID 189:201)           
REMARK   3    ORIGIN FOR THE GROUP (A): -17.3015   1.5829   4.8188              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2334 T22:   0.3667                                     
REMARK   3      T33:   0.3293 T12:   0.0982                                     
REMARK   3      T13:   0.0584 T23:  -0.0253                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   9.2195 L22:   3.7052                                     
REMARK   3      L33:   7.7221 L12:   0.0637                                     
REMARK   3      L13:   1.2700 L23:   0.3269                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0501 S12:   0.3235 S13:  -0.0417                       
REMARK   3      S21:  -0.3178 S22:  -0.0383 S23:  -0.5267                       
REMARK   3      S31:  -0.0860 S32:   0.7443 S33:  -0.0442                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN A AND (RESID 158:188)                            
REMARK   3    ORIGIN FOR THE GROUP (A):  -8.2456   0.5687  15.0693              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4320 T22:   0.7340                                     
REMARK   3      T33:   0.7265 T12:   0.1834                                     
REMARK   3      T13:  -0.1193 T23:  -0.1098                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.4949 L22:   3.6792                                     
REMARK   3      L33:   3.9600 L12:  -2.9824                                     
REMARK   3      L13:  -1.0535 L23:   3.5009                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2245 S12:  -0.4398 S13:  -0.3924                       
REMARK   3      S21:   0.9184 S22:   1.0222 S23:  -1.1120                       
REMARK   3      S31:   0.8054 S32:   1.5723 S33:  -0.5092                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN B AND (RESID 114:157 OR RESID 189:201)           
REMARK   3    ORIGIN FOR THE GROUP (A): -32.5961  -1.5849   4.8563              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2060 T22:   0.4143                                     
REMARK   3      T33:   0.3396 T12:   0.1070                                     
REMARK   3      T13:  -0.0791 T23:   0.0264                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.5423 L22:   8.9365                                     
REMARK   3      L33:   7.4709 L12:   0.3145                                     
REMARK   3      L13:  -1.4236 L23:  -0.8630                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0416 S12:   0.2918 S13:   0.1603                       
REMARK   3      S21:  -0.4199 S22:  -0.0595 S23:   0.6680                       
REMARK   3      S31:   0.1308 S32:  -0.7467 S33:  -0.0032                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN B AND (RESID 158:188)                            
REMARK   3    ORIGIN FOR THE GROUP (A): -41.6391  -0.5532  15.0624              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4199 T22:   0.7458                                     
REMARK   3      T33:   0.7531 T12:   0.1816                                     
REMARK   3      T13:   0.1350 T23:   0.1345                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.7797 L22:   6.0386                                     
REMARK   3      L33:   3.1446 L12:  -4.2535                                     
REMARK   3      L13:   0.9646 L23:  -2.6280                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0990 S12:  -0.5228 S13:   0.4530                       
REMARK   3      S21:   0.9024 S22:   0.8404 S23:   1.3544                       
REMARK   3      S31:  -0.5551 S32:  -1.3149 S33:  -0.4308                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5M2C COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 12-OCT-16.                  
REMARK 100 THE DEPOSITION ID IS D_1200001768.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 01-OCT-12                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5                                  
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : DIAMOND                            
REMARK 200  BEAMLINE                       : I03                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9763                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS3 6M                
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 13921                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.960                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 41.100                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY                : 9.600                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 50.4000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.96                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.02                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 9.60                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.92300                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 3.200                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 1G8Q                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 42.16                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.13                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 29% ETOH, 0.092 M CITRATE PH 2.2,        
REMARK 280  0.113 M NA2HPO4 PH 9.3, (PH ~5) 39% PEG 300, PH 5, VAPOR            
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 294.15K                        
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 32 1 2                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+2/3                                            
REMARK 290       3555   -X+Y,-X,Z+1/3                                           
REMARK 290       4555   -Y,-X,-Z+1/3                                            
REMARK 290       5555   -X+Y,Y,-Z+2/3                                           
REMARK 290       6555   X,X-Y,-Z                                                
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       88.29267            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       44.14633            
REMARK 290   SMTRY1   4  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   4 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000       44.14633            
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000       88.29267            
REMARK 290   SMTRY1   6  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2030 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 8780 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -23.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375      HOH A 409  LIES ON A SPECIAL POSITION.                          
REMARK 375      HOH B 411  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLU A   110                                                      
REMARK 465     THR A   111                                                      
REMARK 465     GLY A   112                                                      
REMARK 465     PHE A   113                                                      
REMARK 465     GLY A   202                                                      
REMARK 465     THR A   203                                                      
REMARK 465     LYS A   204                                                      
REMARK 465     HIS A   205                                                      
REMARK 465     HIS A   206                                                      
REMARK 465     HIS A   207                                                      
REMARK 465     HIS A   208                                                      
REMARK 465     HIS A   209                                                      
REMARK 465     HIS A   210                                                      
REMARK 465     GLU B   110                                                      
REMARK 465     THR B   111                                                      
REMARK 465     GLY B   112                                                      
REMARK 465     PHE B   113                                                      
REMARK 465     GLY B   202                                                      
REMARK 465     THR B   203                                                      
REMARK 465     LYS B   204                                                      
REMARK 465     HIS B   205                                                      
REMARK 465     HIS B   206                                                      
REMARK 465     HIS B   207                                                      
REMARK 465     HIS B   208                                                      
REMARK 465     HIS B   209                                                      
REMARK 465     HIS B   210                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ALA A 140       81.93    -68.44                                   
REMARK 500    CYS A 157       22.99   -145.58                                   
REMARK 500    CYS B 157       25.63   -148.11                                   
REMARK 500    LYS B 171       58.73   -147.66                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 A 301                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 B 301                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1G8Q   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 1IV5   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5M3D   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5M4R   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5M33   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5M3T   RELATED DB: PDB                                   
DBREF  5M2C A  112   201  UNP    P60033   CD81_HUMAN     112    201             
DBREF  5M2C B  112   201  UNP    P60033   CD81_HUMAN     112    201             
SEQADV 5M2C GLU A  110  UNP  P60033              CLONING ARTIFACT               
SEQADV 5M2C THR A  111  UNP  P60033              CLONING ARTIFACT               
SEQADV 5M2C GLY A  202  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C THR A  203  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C LYS A  204  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS A  205  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS A  206  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS A  207  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS A  208  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS A  209  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS A  210  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C GLU B  110  UNP  P60033              CLONING ARTIFACT               
SEQADV 5M2C THR B  111  UNP  P60033              CLONING ARTIFACT               
SEQADV 5M2C GLY B  202  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C THR B  203  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C LYS B  204  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS B  205  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS B  206  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS B  207  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS B  208  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS B  209  UNP  P60033              EXPRESSION TAG                 
SEQADV 5M2C HIS B  210  UNP  P60033              EXPRESSION TAG                 
SEQRES   1 A  101  GLU THR GLY PHE VAL ASN LYS ASP GLN ILE ALA LYS ASP          
SEQRES   2 A  101  VAL LYS GLN PHE TYR ASP GLN ALA LEU GLN GLN ALA VAL          
SEQRES   3 A  101  VAL ASP ASP ASP ALA ASN ASN ALA LYS ALA VAL VAL LYS          
SEQRES   4 A  101  THR PHE HIS GLU THR LEU ASP CYS CYS GLY SER SER THR          
SEQRES   5 A  101  LEU THR ALA LEU THR THR SER VAL LEU LYS ASN ASN LEU          
SEQRES   6 A  101  CYS PRO SER GLY SER ASN ILE ILE SER ASN LEU PHE LYS          
SEQRES   7 A  101  GLU ASP CYS HIS GLN LYS ILE ASP ASP LEU PHE SER GLY          
SEQRES   8 A  101  LYS GLY THR LYS HIS HIS HIS HIS HIS HIS                      
SEQRES   1 B  101  GLU THR GLY PHE VAL ASN LYS ASP GLN ILE ALA LYS ASP          
SEQRES   2 B  101  VAL LYS GLN PHE TYR ASP GLN ALA LEU GLN GLN ALA VAL          
SEQRES   3 B  101  VAL ASP ASP ASP ALA ASN ASN ALA LYS ALA VAL VAL LYS          
SEQRES   4 B  101  THR PHE HIS GLU THR LEU ASP CYS CYS GLY SER SER THR          
SEQRES   5 B  101  LEU THR ALA LEU THR THR SER VAL LEU LYS ASN ASN LEU          
SEQRES   6 B  101  CYS PRO SER GLY SER ASN ILE ILE SER ASN LEU PHE LYS          
SEQRES   7 B  101  GLU ASP CYS HIS GLN LYS ILE ASP ASP LEU PHE SER GLY          
SEQRES   8 B  101  LYS GLY THR LYS HIS HIS HIS HIS HIS HIS                      
HET    PO4  A 301       5                                                       
HET    PO4  B 301       5                                                       
HETNAM     PO4 PHOSPHATE ION                                                    
FORMUL   3  PO4    2(O4 P 3-)                                                   
FORMUL   5  HOH   *35(H2 O)                                                     
HELIX    1 AA1 ASN A  115  ASP A  137  1                                  23    
HELIX    2 AA2 ALA A  140  ASP A  155  1                                  16    
HELIX    3 AA3 SER A  160  LYS A  171  1                                  12    
HELIX    4 AA4 ASN A  172  CYS A  175  5                                   4    
HELIX    5 AA5 ASN A  180  LEU A  185  1                                   6    
HELIX    6 AA6 ASP A  189  GLY A  200  1                                  12    
HELIX    7 AA7 ASN B  115  ASP B  137  1                                  23    
HELIX    8 AA8 ALA B  140  ASP B  155  1                                  16    
HELIX    9 AA9 SER B  160  LYS B  171  1                                  12    
HELIX   10 AB1 ASN B  172  CYS B  175  5                                   4    
HELIX   11 AB2 ASN B  180  LYS B  187  1                                   8    
HELIX   12 AB3 ASP B  189  GLY B  200  1                                  12    
SSBOND   1 CYS A  156    CYS A  190                          1555   1555  2.01  
SSBOND   2 CYS A  157    CYS A  175                          1555   1555  2.02  
SSBOND   3 CYS B  156    CYS B  190                          1555   1555  2.02  
SSBOND   4 CYS B  157    CYS B  175                          1555   1555  2.01  
SITE     1 AC1  4 LYS A 124  HIS A 191  ASP A 195  HOH A 401                    
SITE     1 AC2  6 LYS B 121  LYS B 124  HIS B 191  ASP B 195                    
SITE     2 AC2  6 HOH B 402  HOH B 403                                          
CRYST1   49.879   49.879  132.439  90.00  90.00 120.00 P 32 1 2     12          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.020049  0.011575  0.000000        0.00000                         
SCALE2      0.000000  0.023150  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.007551        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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