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Database: PDB
Entry: 5M72
LinkDB: 5M72
Original site: 5M72 
HEADER    PROTEIN TRANSPORT                       26-OCT-16   5M72              
TITLE     STRUCTURE OF THE HUMAN SRP68-72 PROTEIN-BINDING DOMAIN COMPLEX        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: SIGNAL RECOGNITION PARTICLE SUBUNIT SRP72;                 
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: SRP72,SIGNAL RECOGNITION PARTICLE 72 KDA PROTEIN;           
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68;                 
COMPND   8 CHAIN: B;                                                            
COMPND   9 SYNONYM: SRP68,SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN;           
COMPND  10 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: SRP72;                                                         
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  10 ORGANISM_COMMON: HUMAN;                                              
SOURCE  11 ORGANISM_TAXID: 9606;                                                
SOURCE  12 GENE: SRP68;                                                         
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    PROTEIN TARGETING, SIGNAL RECOGNITION PARTICLE, TETRATRICOPEPTIDE     
KEYWDS   2 REPEAT, PROTEIN-PEPTIDE COMPLEX, PROTEIN TRANSPORT                   
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    M.M.M.BECKER,K.WILD,I.SINNING                                         
REVDAT   4   18-JAN-17 5M72    1       JRNL                                     
REVDAT   3   21-DEC-16 5M72    1       JRNL                                     
REVDAT   2   14-DEC-16 5M72    1       JRNL                                     
REVDAT   1   07-DEC-16 5M72    0                                                
JRNL        AUTH   M.M.BECKER,K.LAPOUGE,B.SEGNITZ,K.WILD,I.SINNING              
JRNL        TITL   STRUCTURES OF HUMAN SRP72 COMPLEXES PROVIDE INSIGHTS INTO    
JRNL        TITL 2 SRP RNA REMODELING AND RIBOSOME INTERACTION.                 
JRNL        REF    NUCLEIC ACIDS RES.            V.  45   470 2017              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   27899666                                                     
JRNL        DOI    10.1093/NAR/GKW1124                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.60 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.10.1_2155                                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.60                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 39.00                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.330                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.3                           
REMARK   3   NUMBER OF REFLECTIONS             : 25062                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.145                           
REMARK   3   R VALUE            (WORKING SET) : 0.143                           
REMARK   3   FREE R VALUE                     : 0.184                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.220                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1308                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 39.0431 -  3.3276    0.99     2690   150  0.1437 0.1740        
REMARK   3     2  3.3276 -  2.6414    1.00     2643   151  0.1514 0.1822        
REMARK   3     3  2.6414 -  2.3075    0.99     2656   145  0.1389 0.1708        
REMARK   3     4  2.3075 -  2.0966    0.99     2633   131  0.1203 0.2007        
REMARK   3     5  2.0966 -  1.9463    1.00     2663   133  0.1212 0.1688        
REMARK   3     6  1.9463 -  1.8315    0.99     2598   157  0.1301 0.1957        
REMARK   3     7  1.8315 -  1.7398    0.99     2618   141  0.1550 0.2218        
REMARK   3     8  1.7398 -  1.6641    1.00     2620   144  0.1643 0.2112        
REMARK   3     9  1.6641 -  1.6000    0.99     2633   156  0.1911 0.2480        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.150            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 21.970           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 36.16                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.005           1453                                  
REMARK   3   ANGLE     :  0.704           1969                                  
REMARK   3   CHIRALITY :  0.040            222                                  
REMARK   3   PLANARITY :  0.004            254                                  
REMARK   3   DIHEDRAL  : 11.264            899                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5M72 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 26-OCT-16.                  
REMARK 100 THE DEPOSITION ID IS D_1200001290.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 27-JUL-15                          
REMARK 200  TEMPERATURE           (KELVIN) : 80                                 
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ESRF                               
REMARK 200  BEAMLINE                       : ID23-1                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.972422                           
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M-F               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : AIMLESS                            
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 25085                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.600                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 39.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.4                               
REMARK 200  DATA REDUNDANCY                : 5.000                              
REMARK 200  R MERGE                    (I) : 0.05700                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 14.2000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.55000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: SAD                          
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 49.87                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.45                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M K2SO4 20% (W/V) PEG 3350, VAPOR    
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 291.15K                        
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       33.29850            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       26.13450            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       33.29850            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       26.13450            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 3390 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 9520 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -48.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375 K      K A 203  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A     9                                                      
REMARK 465     ASN A   160                                                      
REMARK 465     TRP A   161                                                      
REMARK 465     GLU A   162                                                      
REMARK 465     LYS A   163                                                      
REMARK 465     VAL A   164                                                      
REMARK 465     VAL A   165                                                      
REMARK 465     PRO A   166                                                      
REMARK 465     GLY A   167                                                      
REMARK 465     SER A   168                                                      
REMARK 465     MET B   544                                                      
REMARK 465     GLY B   545                                                      
REMARK 465     SER B   546                                                      
REMARK 465     GLN B   547                                                      
REMARK 465     VAL B   548                                                      
REMARK 465     LYS B   549                                                      
REMARK 465     ASP B   550                                                      
REMARK 465     ASN B   551                                                      
REMARK 465     LYS B   552                                                      
REMARK 465     PRO B   553                                                      
REMARK 465     LEU B   554                                                      
REMARK 465     VAL B   555                                                      
REMARK 465     GLU B   556                                                      
REMARK 465     ARG B   557                                                      
REMARK 465     PHE B   558                                                      
REMARK 465     GLU B   559                                                      
REMARK 465     THR B   560                                                      
REMARK 465     PHE B   561                                                      
REMARK 465     CYS B   562                                                      
REMARK 465     LEU B   563                                                      
REMARK 465     ASP B   564                                                      
REMARK 465     PRO B   565                                                      
REMARK 465     SER B   566                                                      
REMARK 465     LEU B   567                                                      
REMARK 465     VAL B   568                                                      
REMARK 465     THR B   569                                                      
REMARK 465     LYS B   570                                                      
REMARK 465     GLN B   571                                                      
REMARK 465     ALA B   572                                                      
REMARK 465     ASN B   573                                                      
REMARK 465     LEU B   574                                                      
REMARK 465     VAL B   575                                                      
REMARK 465     HIS B   576                                                      
REMARK 465     PHE B   577                                                      
REMARK 465     PRO B   578                                                      
REMARK 465     PRO B   579                                                      
REMARK 465     GLY B   580                                                      
REMARK 465     PHE B   581                                                      
REMARK 465     GLN B   582                                                      
REMARK 465     PRO B   583                                                      
REMARK 465     ILE B   584                                                      
REMARK 465     PRO B   585                                                      
REMARK 465     CYS B   586                                                      
REMARK 465     GLU B   608                                                      
REMARK 465     GLN B   609                                                      
REMARK 465     LYS B   610                                                      
REMARK 465     THR B   611                                                      
REMARK 465     LYS B   612                                                      
REMARK 465     SER B   613                                                      
REMARK 465     GLY B   614                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   OE2  GLU A    63     O    HOH A   301              2.11            
REMARK 500   OE1  GLU A    17     O1   GOL A   204              2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN A  77       -9.81     72.73                                   
REMARK 500    ASN A  78       30.08    -94.33                                   
REMARK 500    LEU A  80       51.37   -110.93                                   
REMARK 500    ARG A  93       64.73   -117.02                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 ASN A   78     SER A   79                 -140.28                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                             
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                               K A 203   K                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 GLN A  57   O                                                      
REMARK 620 2 ASN A  58   OD1  79.2                                              
REMARK 620 3 HOH A 340   O    73.9 116.5                                        
REMARK 620 4 HOH A 369   O   115.0  53.1  89.6                                  
REMARK 620 5 GLN A  57   O     0.0  79.2  73.9 115.0                            
REMARK 620 6 ASN A  58   OD1  79.2   0.0 116.5  53.1  79.2                      
REMARK 620 7 HOH A 369   O    65.9 130.2  87.5 176.5  65.9 130.2                
REMARK 620 8 HOH A 340   O   135.5 138.9  68.1  87.5 135.5 138.9  89.6          
REMARK 620 N                    1     2     3     4     5     6     7           
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 201                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 202                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue K A 203                   
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 204                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 205                 
DBREF  5M72 A   10   166  UNP    O76094   SRP72_HUMAN     10    166             
DBREF  5M72 B  546   614  UNP    Q9UHB9   SRP68_HUMAN    546    614             
SEQADV 5M72 GLY A    9  UNP  O76094              EXPRESSION TAG                 
SEQADV 5M72 GLY A  167  UNP  O76094              EXPRESSION TAG                 
SEQADV 5M72 SER A  168  UNP  O76094              EXPRESSION TAG                 
SEQADV 5M72 MET B  544  UNP  Q9UHB9              INITIATING METHIONINE          
SEQADV 5M72 GLY B  545  UNP  Q9UHB9              EXPRESSION TAG                 
SEQRES   1 A  160  GLY SER VAL PRO ALA LEU TRP SER GLU VAL ASN ARG TYR          
SEQRES   2 A  160  GLY GLN ASN GLY ASP PHE THR ARG ALA LEU LYS THR VAL          
SEQRES   3 A  160  ASN LYS ILE LEU GLN ILE ASN LYS ASP ASP VAL THR ALA          
SEQRES   4 A  160  LEU HIS CYS LYS VAL VAL CYS LEU ILE GLN ASN GLY SER          
SEQRES   5 A  160  PHE LYS GLU ALA LEU ASN VAL ILE ASN THR HIS THR LYS          
SEQRES   6 A  160  VAL LEU ALA ASN ASN SER LEU SER PHE GLU LYS ALA TYR          
SEQRES   7 A  160  CYS GLU TYR ARG LEU ASN ARG ILE GLU ASN ALA LEU LYS          
SEQRES   8 A  160  THR ILE GLU SER ALA ASN GLN GLN THR ASP LYS LEU LYS          
SEQRES   9 A  160  GLU LEU TYR GLY GLN VAL LEU TYR ARG LEU GLU ARG TYR          
SEQRES  10 A  160  ASP GLU CYS LEU ALA VAL TYR ARG ASP LEU VAL ARG ASN          
SEQRES  11 A  160  SER GLN ASP ASP TYR ASP GLU GLU ARG LYS THR ASN LEU          
SEQRES  12 A  160  SER ALA VAL VAL ALA ALA GLN SER ASN TRP GLU LYS VAL          
SEQRES  13 A  160  VAL PRO GLY SER                                              
SEQRES   1 B   71  MET GLY SER GLN VAL LYS ASP ASN LYS PRO LEU VAL GLU          
SEQRES   2 B   71  ARG PHE GLU THR PHE CYS LEU ASP PRO SER LEU VAL THR          
SEQRES   3 B   71  LYS GLN ALA ASN LEU VAL HIS PHE PRO PRO GLY PHE GLN          
SEQRES   4 B   71  PRO ILE PRO CYS LYS PRO LEU PHE PHE ASP LEU ALA LEU          
SEQRES   5 B   71  ASN HIS VAL ALA PHE PRO PRO LEU GLU ASP LYS LEU GLU          
SEQRES   6 B   71  GLN LYS THR LYS SER GLY                                      
HET    SO4  A 201       5                                                       
HET    SO4  A 202       5                                                       
HET      K  A 203       1                                                       
HET    GOL  A 204       6                                                       
HET    GOL  A 205       6                                                       
HETNAM     SO4 SULFATE ION                                                      
HETNAM       K POTASSIUM ION                                                    
HETNAM     GOL GLYCEROL                                                         
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL                                    
FORMUL   3  SO4    2(O4 S 2-)                                                   
FORMUL   5    K    K 1+                                                         
FORMUL   6  GOL    2(C3 H8 O3)                                                  
FORMUL   8  HOH   *150(H2 O)                                                    
HELIX    1 AA1 SER A   10  ASN A   24  1                                  15    
HELIX    2 AA2 ASP A   26  ASN A   41  1                                  16    
HELIX    3 AA3 ASP A   44  ASN A   58  1                                  15    
HELIX    4 AA4 SER A   60  HIS A   71  1                                  12    
HELIX    5 AA5 HIS A   71  ALA A   76  1                                   6    
HELIX    6 AA6 LEU A   80  LEU A   91  1                                  12    
HELIX    7 AA7 ARG A   93  SER A  103  1                                  11    
HELIX    8 AA8 THR A  108  LEU A  122  1                                  15    
HELIX    9 AA9 ARG A  124  SER A  139  1                                  16    
HELIX   10 AB1 TYR A  143  SER A  159  1                                  17    
HELIX   11 AB2 ASP B  592  VAL B  598  5                                   7    
HELIX   12 AB3 LEU B  603  LEU B  607  5                                   5    
LINK         O   GLN A  57                 K     K A 203     1555   1555  2.77  
LINK         OD1 ASN A  58                 K     K A 203     1555   1555  2.75  
LINK         K     K A 203                 O   HOH A 340     1555   1555  2.82  
LINK         K     K A 203                 O   HOH A 369     1555   1555  3.49  
LINK         O   GLN A  57                 K     K A 203     1555   2656  2.77  
LINK         OD1 ASN A  58                 K     K A 203     1555   2656  2.75  
LINK         K     K A 203                 O   HOH A 369     1555   2656  3.49  
LINK         K     K A 203                 O   HOH A 340     1555   2656  2.82  
SITE     1 AC1  3 ARG A  29  ARG A 121  HOH A 366                               
SITE     1 AC2  6 ASN A  77  SER A  79  SER A  81  ALA A 104                    
SITE     2 AC2  6 ASN A 105  GLN A 106                                          
SITE     1 AC3  3 GLN A  57  ASN A  58  HOH A 340                               
SITE     1 AC4  6 GLU A  17  ARG A  20  TYR A  21  HOH A 305                    
SITE     2 AC4  6 PHE B 590  PHE B 591                                          
SITE     1 AC5  3 GLN A 107  LYS A 112  TYR A 115                               
CRYST1   66.597   52.269   62.863  90.00 118.22  90.00 C 1 2 1       4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.015016  0.000000  0.008056        0.00000                         
SCALE2      0.000000  0.019132  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.018053        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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