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Database: PDB
Entry: 5NWX
LinkDB: 5NWX
Original site: 5NWX 
HEADER    TRANSCRIPTION                           08-MAY-17   5NWX              
TITLE     INSIGHT INTO THE MOLECULAR RECOGNITION MECHANISM OF THE COACTIVATOR   
TITLE    2 NCOA1 BY STAT6                                                       
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: NUCLEAR RECEPTOR COACTIVATOR 1;                            
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: UNP RESIDUES  257-385;                                     
COMPND   5 SYNONYM: NCOA-1,NUCLEAR RECEPTOR COACTIVATOR PROTEIN 1,MNRC-1,STEROID
COMPND   6 RECEPTOR COACTIVATOR 1,SRC-1;                                        
COMPND   7 EC: 2.3.1.48;                                                        
COMPND   8 ENGINEERED: YES;                                                     
COMPND   9 MUTATION: YES;                                                       
COMPND  10 MOL_ID: 2;                                                           
COMPND  11 MOLECULE: SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 6;        
COMPND  12 CHAIN: B;                                                            
COMPND  13 FRAGMENT: UNP RESIDUES 783-814;                                      
COMPND  14 SYNONYM: IL-4 STAT;                                                  
COMPND  15 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: MOUSE;                                              
SOURCE   4 ORGANISM_TAXID: 10090;                                               
SOURCE   5 GENE: NCOA1, SRC1;                                                   
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PET16B;                               
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  11 ORGANISM_COMMON: HUMAN;                                              
SOURCE  12 ORGANISM_TAXID: 9606;                                                
SOURCE  13 GENE: STAT6;                                                         
SOURCE  14 EXPRESSION_SYSTEM: SYNTHETIC CONSTRUCT;                              
SOURCE  15 EXPRESSION_SYSTEM_TAXID: 32630                                       
KEYWDS    NCOA1, STAT6, PAS-B DOMAIN, TRANSACTIVATION DOMAIN, TRANSCRIPTION     
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    L.RUSSO,K.GILLER,E.PFITZNER,C.GRIESINGER,S.BECKER                     
REVDAT   1   13-DEC-17 5NWX    0                                                
JRNL        AUTH   L.RUSSO,K.GILLER,E.PFITZNER,C.GRIESINGER,S.BECKER            
JRNL        TITL   INSIGHT INTO THE MOLECULAR RECOGNITION MECHANISM OF THE      
JRNL        TITL 2 COACTIVATOR NCOA1 BY STAT6.                                  
JRNL        REF    SCI REP                       V.   7 16845 2017              
JRNL        REFN                   ESSN 2045-2322                               
JRNL        PMID   29203888                                                     
JRNL        DOI    10.1038/S41598-017-17088-5                                   
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   A.RAZETO,V.RAMAKRISHNAN,C.M.LITTERST,K.GILLER,C.GRIESINGER,  
REMARK   1  AUTH 2 T.CARLOMAGNO,N.LAKOMEK,T.HEIMBURG,M.LODRINI,E.PFITZNER,      
REMARK   1  AUTH 3 S.BECKER                                                     
REMARK   1  TITL   STRUCTURE OF THE NCOA-1/SRC-1 PAS-B DOMAIN BOUND TO THE      
REMARK   1  TITL 2 LXXLL MOTIF OF THE STAT6 TRANSACTIVATION DOMAIN              
REMARK   1  REF    JOURNAL OF MOLECULAR BIOLOGY  V. 336   319 2004              
REMARK   1  REFN                   ISSN 0022-2836                               
REMARK   1  PMID   14757047                                                     
REMARK   1  DOI    10.1016/J.JMB.2003.12.057                                    
REMARK   1 REFERENCE 2                                                          
REMARK   1  AUTH   A.RAZETO,E.PFITZNER,S.BECKER                                 
REMARK   1  TITL   CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF  
REMARK   1  TITL 2 THE NCOA-1/SRC-1 PAS-B DOMAIN BOUND TO THE LXXLL MOTIF OF    
REMARK   1  TITL 3 THE STAT6 TRANSACTIVATION DOMAIN                             
REMARK   1  REF    ACTA CRYSTALLOGRAPHICA        V. D60   550 2004              
REMARK   1  REF  2 SECTION D                                                    
REMARK   1  REFN                   ISSN 0907-4449                               
REMARK   1  PMID   14993689                                                     
REMARK   1  DOI    10.1107/S0907444903029378                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.51 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.6.0117                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.51                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 53.37                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 5203                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.186                           
REMARK   3   R VALUE            (WORKING SET) : 0.184                           
REMARK   3   FREE R VALUE                     : 0.235                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.500                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 245                             
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.51                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.58                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 359                          
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 96.86                        
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2580                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 11                           
REMARK   3   BIN FREE R VALUE                    : 0.3200                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 868                                     
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 8                                       
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 46.36                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 1.19000                                              
REMARK   3    B22 (A**2) : 1.19000                                              
REMARK   3    B33 (A**2) : -1.78000                                             
REMARK   3    B12 (A**2) : 0.59000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): 0.341         
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.248         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.208         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 9.917         
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.958                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.930                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):   893 ; 0.016 ; 0.020       
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  1211 ; 1.948 ; 1.967       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   114 ; 7.447 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):    33 ;27.805 ;23.333       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   142 ;18.208 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):     5 ;25.244 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   142 ; 0.124 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):   661 ; 0.007 ; 0.021       
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN ANGLE OTHER ATOMS   (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   LONG RANGE B REFINED ATOMS     (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   LONG RANGE B OTHER ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : NULL                                                 
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.20                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN USED IF PRESENT IN    
REMARK   3  THE INPUT                                                           
REMARK   4                                                                      
REMARK   4 5NWX COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 08-MAY-17.                  
REMARK 100 THE DEPOSITION ID IS D_1200004865.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 27-NOV-10                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SLS                                
REMARK 200  BEAMLINE                       : X10SA                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.7                                
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 225 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : SADABS                             
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 5471                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.510                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 53.370                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8                               
REMARK 200  DATA REDUNDANCY                : 20.51                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 30.2600                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.51                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.61                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 98.3                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 19.92                              
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 1OJ5                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 44.09                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.25                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M SODIUM ACETATE, 0.1 M SODIUM       
REMARK 280  CACODYLATE, PH 6.5, 30% PEG 8000, 5% PEG 400, VAPOR DIFFUSION,      
REMARK 280  HANGING DROP, TEMPERATURE 293K                                      
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 62                             
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+2/3                                            
REMARK 290       3555   -X+Y,-X,Z+1/3                                           
REMARK 290       4555   -X,-Y,Z                                                 
REMARK 290       5555   Y,-X+Y,Z+2/3                                            
REMARK 290       6555   X-Y,X,Z+1/3                                             
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       48.85533            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       24.42767            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000       48.85533            
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       24.42767            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1000 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 6460 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -10.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A   254                                                      
REMARK 465     HIS A   255                                                      
REMARK 465     MET A   256                                                      
REMARK 465     THR A   257                                                      
REMARK 465     GLY A   258                                                      
REMARK 465     GLN A   350                                                      
REMARK 465     SER A   351                                                      
REMARK 465     PRO A   352                                                      
REMARK 465     ASP A   353                                                      
REMARK 465     MET A   354                                                      
REMARK 465     GLU A   367                                                      
REMARK 465     HIS A   368                                                      
REMARK 465     SER A   369                                                      
REMARK 465     GLY A   370                                                      
REMARK 465     LEU A   371                                                      
REMARK 465     SER A   372                                                      
REMARK 465     PRO A   373                                                      
REMARK 465     GLN A   374                                                      
REMARK 465     ASP A   375                                                      
REMARK 465     ASP A   376                                                      
REMARK 465     THR A   377                                                      
REMARK 465     ASN A   378                                                      
REMARK 465     SER A   379                                                      
REMARK 465     GLY A   380                                                      
REMARK 465     MET A   381                                                      
REMARK 465     SER A   382                                                      
REMARK 465     ILE A   383                                                      
REMARK 465     PRO A   384                                                      
REMARK 465     ARG A   385                                                      
REMARK 465     GLY B   783                                                      
REMARK 465     THR B   784                                                      
REMARK 465     TRP B   785                                                      
REMARK 465     ILE B   786                                                      
REMARK 465     GLY B   787                                                      
REMARK 465     GLU B   788                                                      
REMARK 465     ASP B   789                                                      
REMARK 465     ILE B   790                                                      
REMARK 465     PHE B   791                                                      
REMARK 465     PRO B   792                                                      
REMARK 465     PRO B   793                                                      
REMARK 465     GLY B   809                                                      
REMARK 465     GLN B   810                                                      
REMARK 465     GLY B   811                                                      
REMARK 465     GLU B   812                                                      
REMARK 465     SER B   813                                                      
REMARK 465     GLY B   814                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     VAL A 259    N    CA   CB   CG1  CG2                             
REMARK 470     LYS A 265    CG   CD   CE   NZ                                   
REMARK 470     LYS A 271    CE   NZ                                             
REMARK 470     TYR A 297    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     ARG A 305    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 306    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 311    CD   NE   CZ   NH1  NH2                             
REMARK 470     GLN A 315    CD   OE1  NE2                                       
REMARK 470     ARG A 329    CZ   NH1  NH2                                       
REMARK 470     ARG A 343    CZ   NH1  NH2                                       
REMARK 470     LYS A 345    CG   CD   CE   NZ                                   
REMARK 470     PRO A 349    CA   C    O    CB   CG   CD                         
REMARK 470     GLN A 355    N    CA   CB   CG   CD   OE1  NE2                   
REMARK 470     ILE A 361    CD1                                                 
REMARK 470     GLU B 799    CG   CD   OE1  OE2                                  
REMARK 470     GLN B 800    CG   CD   OE1  NE2                                  
REMARK 470     LYS B 804    CD   CE   NZ                                        
REMARK 470     LEU B 807    CG   CD1  CD2                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS B 804      -71.75    -39.38                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5NWM   RELATED DB: PDB                                   
REMARK 900 NMR STRUCTURE OF THE NCOA1-STAT6 COMPLEX                             
DBREF  5NWX A  257   385  UNP    P70365   NCOA1_MOUSE    257    385             
DBREF  5NWX B  783   814  UNP    P42226   STAT6_HUMAN    783    814             
SEQADV 5NWX GLY A  254  UNP  P70365              EXPRESSION TAG                 
SEQADV 5NWX HIS A  255  UNP  P70365              EXPRESSION TAG                 
SEQADV 5NWX MET A  256  UNP  P70365              EXPRESSION TAG                 
SEQADV 5NWX ARG A  343  UNP  P70365    LYS   343 CONFLICT                       
SEQADV 5NWX THR A  377  UNP  P70365    SER   377 CONFLICT                       
SEQRES   1 A  132  GLY HIS MET THR GLY VAL GLU SER PHE MET THR LYS GLN          
SEQRES   2 A  132  ASP THR THR GLY LYS ILE ILE SER ILE ASP THR SER SER          
SEQRES   3 A  132  LEU ARG ALA ALA GLY ARG THR GLY TRP GLU ASP LEU VAL          
SEQRES   4 A  132  ARG LYS CYS ILE TYR ALA PHE PHE GLN PRO GLN GLY ARG          
SEQRES   5 A  132  GLU PRO SER TYR ALA ARG GLN LEU PHE GLN GLU VAL MET          
SEQRES   6 A  132  THR ARG GLY THR ALA SER SER PRO SER TYR ARG PHE ILE          
SEQRES   7 A  132  LEU ASN ASP GLY THR MET LEU SER ALA HIS THR ARG CYS          
SEQRES   8 A  132  LYS LEU CYS TYR PRO GLN SER PRO ASP MET GLN PRO PHE          
SEQRES   9 A  132  ILE MET GLY ILE HIS ILE ILE ASP ARG GLU HIS SER GLY          
SEQRES  10 A  132  LEU SER PRO GLN ASP ASP THR ASN SER GLY MET SER ILE          
SEQRES  11 A  132  PRO ARG                                                      
SEQRES   1 B   32  GLY THR TRP ILE GLY GLU ASP ILE PHE PRO PRO LEU LEU          
SEQRES   2 B   32  PRO PRO THR GLU GLN ASP LEU THR LYS LEU LEU LEU GLU          
SEQRES   3 B   32  GLY GLN GLY GLU SER GLY                                      
FORMUL   3  HOH   *8(H2 O)                                                      
HELIX    1 AA1 THR A  277  ALA A  282  1                                   6    
HELIX    2 AA2 GLY A  287  PHE A  300  1                                  14    
HELIX    3 AA3 SER A  308  GLY A  321  1                                  14    
HELIX    4 AA4 THR B  798  GLU B  808  1                                  11    
SHEET    1 AA1 5 ILE A 272  ASP A 276  0                                        
SHEET    2 AA1 5 SER A 261  GLN A 266 -1  N  MET A 263   O  ASP A 276           
SHEET    3 AA1 5 PHE A 357  ASP A 365 -1  O  ILE A 358   N  GLN A 266           
SHEET    4 AA1 5 MET A 337  CYS A 347 -1  N  CYS A 347   O  PHE A 357           
SHEET    5 AA1 5 THR A 322  SER A 324 -1  N  ALA A 323   O  CYS A 344           
SHEET    1 AA2 5 ILE A 272  ASP A 276  0                                        
SHEET    2 AA2 5 SER A 261  GLN A 266 -1  N  MET A 263   O  ASP A 276           
SHEET    3 AA2 5 PHE A 357  ASP A 365 -1  O  ILE A 358   N  GLN A 266           
SHEET    4 AA2 5 MET A 337  CYS A 347 -1  N  CYS A 347   O  PHE A 357           
SHEET    5 AA2 5 TYR A 328  ILE A 331 -1  N  TYR A 328   O  ALA A 340           
CRYST1   61.631   61.631   73.283  90.00  90.00 120.00 P 62          6          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.016225  0.009368  0.000000        0.00000                         
SCALE2      0.000000  0.018736  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.013646        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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