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Database: PDB
Entry: 5OJ8
LinkDB: 5OJ8
Original site: 5OJ8 
HEADER    MOTOR PROTEIN                           20-JUL-17   5OJ8              
TITLE     CRYSTAL STRUCTURE OF THE KLC1-TPR DOMAIN ([A1-B5] FRAGMENT)           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: KINESIN LIGHT CHAIN 1;                                     
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: KLC 1;                                                      
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: KLC1, KLC, KNS2;                                               
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    KINESIN1, KLC1, TPR DOMAIN, MOTOR PROTEIN                             
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    T.Q.NGUYEN,M.CHENON,F.VILELA,C.VELOURS,P.FERNANDEZ-VARELA,P.LLINAS,   
AUTHOR   2 J.MENETREY                                                           
REVDAT   2   10-JAN-18 5OJ8    1       JRNL                                     
REVDAT   1   11-OCT-17 5OJ8    0                                                
JRNL        AUTH   T.Q.NGUYEN,M.CHENON,F.VILELA,C.VELOURS,M.AUMONT-NICAISE,     
JRNL        AUTH 2 J.ANDREANI,P.F.VARELA,P.LLINAS,J.MENETREY                    
JRNL        TITL   STRUCTURAL PLASTICITY OF THE N-TERMINAL CAPPING HELIX OF THE 
JRNL        TITL 2 TPR DOMAIN OF KINESIN LIGHT CHAIN.                           
JRNL        REF    PLOS ONE                      V.  12 86354 2017              
JRNL        REFN                   ESSN 1932-6203                               
JRNL        PMID   29036226                                                     
JRNL        DOI    10.1371/JOURNAL.PONE.0186354                                 
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.25 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.10.1_2155: ???)                            
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.25                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 34.25                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.0                           
REMARK   3   NUMBER OF REFLECTIONS             : 14294                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.218                           
REMARK   3   R VALUE            (WORKING SET) : 0.216                           
REMARK   3   FREE R VALUE                     : 0.248                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.990                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 713                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 34.2540 -  3.8402    0.98     2896   150  0.1791 0.1936        
REMARK   3     2  3.8402 -  3.0487    0.99     2729   144  0.2294 0.2726        
REMARK   3     3  3.0487 -  2.6635    1.00     2690   142  0.2722 0.3720        
REMARK   3     4  2.6635 -  2.4200    1.00     2670   140  0.2608 0.2749        
REMARK   3     5  2.4200 -  2.2466    0.98     2596   137  0.3260 0.3847        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.330            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 26.930           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 67.16                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.009           1715                                  
REMARK   3   ANGLE     :  0.940           2317                                  
REMARK   3   CHIRALITY :  0.047            256                                  
REMARK   3   PLANARITY :  0.006            301                                  
REMARK   3   DIHEDRAL  : 21.686            660                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 5                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 209 THROUGH 228 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  21.5259 -20.6030  11.5640              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6710 T22:   0.7137                                     
REMARK   3      T33:   0.4523 T12:   0.1209                                     
REMARK   3      T13:   0.0728 T23:   0.0455                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.5856 L22:   4.9846                                     
REMARK   3      L33:   5.8592 L12:   0.1358                                     
REMARK   3      L13:   2.6853 L23:   0.1100                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1886 S12:   0.9390 S13:   0.0229                       
REMARK   3      S21:  -0.6152 S22:  -0.0003 S23:  -0.1938                       
REMARK   3      S31:   0.2347 S32:   1.4428 S33:  -0.2195                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 229 THROUGH 270 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  18.6436 -20.5351  21.8844              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6610 T22:   0.3784                                     
REMARK   3      T33:   0.5130 T12:   0.1162                                     
REMARK   3      T13:  -0.0362 T23:   0.0378                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.8686 L22:   1.0912                                     
REMARK   3      L33:   3.1468 L12:  -0.6306                                     
REMARK   3      L13:   0.3834 L23:   0.6792                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0154 S12:  -0.1687 S13:  -0.0494                       
REMARK   3      S21:   0.2846 S22:  -0.1537 S23:  -0.1255                       
REMARK   3      S31:   0.4952 S32:  -0.0214 S33:   0.1685                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 271 THROUGH 331 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  16.4563  -3.6794  25.6369              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7249 T22:   0.4030                                     
REMARK   3      T33:   0.5337 T12:   0.1263                                     
REMARK   3      T13:  -0.1264 T23:   0.0075                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.2012 L22:   2.1152                                     
REMARK   3      L33:   1.6802 L12:  -0.1223                                     
REMARK   3      L13:   0.7798 L23:  -0.6633                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2740 S12:  -0.2475 S13:   0.3900                       
REMARK   3      S21:   0.6772 S22:   0.0166 S23:  -0.1270                       
REMARK   3      S31:  -0.3154 S32:   0.0843 S33:   0.2307                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 332 THROUGH 377 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  15.5510   9.6866  13.5524              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6134 T22:   0.4320                                     
REMARK   3      T33:   0.6808 T12:   0.0290                                     
REMARK   3      T13:  -0.0918 T23:   0.0926                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.7212 L22:   2.3700                                     
REMARK   3      L33:   3.2424 L12:   0.2844                                     
REMARK   3      L13:  -0.0269 L23:  -0.5066                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0224 S12:   0.1513 S13:   0.3541                       
REMARK   3      S21:   0.3902 S22:  -0.0854 S23:  -0.4703                       
REMARK   3      S31:  -0.7231 S32:   0.2907 S33:   0.0016                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 378 THROUGH 418 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  10.0095  12.9885  -0.0200              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7358 T22:   0.5483                                     
REMARK   3      T33:   0.5565 T12:  -0.0594                                     
REMARK   3      T13:  -0.0249 T23:   0.0795                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.1453 L22:   1.3775                                     
REMARK   3      L33:   5.1830 L12:  -0.3831                                     
REMARK   3      L13:   0.1496 L23:  -0.8404                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2434 S12:  -0.0051 S13:   0.0738                       
REMARK   3      S21:  -0.1748 S22:   0.1731 S23:   0.0363                       
REMARK   3      S31:  -0.1797 S32:  -0.4953 S33:   0.1243                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5OJ8 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 21-JUL-17.                  
REMARK 100 THE DEPOSITION ID IS D_1200005905.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 11-JUL-16                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SOLEIL                             
REMARK 200  BEAMLINE                       : PROXIMA 1                          
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.978570                           
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 2M                 
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XDS                                
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 14301                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.247                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 44.660                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.6                               
REMARK 200  DATA REDUNDANCY                : 8.600                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 21.2500                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.25                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.38                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 97.5                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.100                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 3NF1                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 49.56                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.44                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 20 % PEG400, 0,15M PHOSPHATE PH7.25      
REMARK 280  10% PEG10K, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K         
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 62 2 2                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+2/3                                            
REMARK 290       3555   -X+Y,-X,Z+1/3                                           
REMARK 290       4555   -X,-Y,Z                                                 
REMARK 290       5555   Y,-X+Y,Z+2/3                                            
REMARK 290       6555   X-Y,X,Z+1/3                                             
REMARK 290       7555   Y,X,-Z+2/3                                              
REMARK 290       8555   X-Y,-Y,-Z                                               
REMARK 290       9555   -X,-X+Y,-Z+1/3                                          
REMARK 290      10555   -Y,-X,-Z+2/3                                            
REMARK 290      11555   -X+Y,Y,-Z                                               
REMARK 290      12555   X,X-Y,-Z+1/3                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000      133.15333            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       66.57667            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000      133.15333            
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       66.57667            
REMARK 290   SMTRY1   7 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   7  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000      133.15333            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   9 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   9 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   9  0.000000  0.000000 -1.000000       66.57667            
REMARK 290   SMTRY1  10  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  10 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  10  0.000000  0.000000 -1.000000      133.15333            
REMARK 290   SMTRY1  11 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2  11  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3  11  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1  12  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  12  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  12  0.000000  0.000000 -1.000000       66.57667            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375 P    PO4 A 501  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A   164                                                      
REMARK 465     GLY A   165                                                      
REMARK 465     SER A   166                                                      
REMARK 465     SER A   167                                                      
REMARK 465     HIS A   168                                                      
REMARK 465     HIS A   169                                                      
REMARK 465     HIS A   170                                                      
REMARK 465     HIS A   171                                                      
REMARK 465     HIS A   172                                                      
REMARK 465     HIS A   173                                                      
REMARK 465     SER A   174                                                      
REMARK 465     SER A   175                                                      
REMARK 465     GLY A   176                                                      
REMARK 465     LEU A   177                                                      
REMARK 465     VAL A   178                                                      
REMARK 465     PRO A   179                                                      
REMARK 465     ARG A   180                                                      
REMARK 465     GLY A   181                                                      
REMARK 465     SER A   182                                                      
REMARK 465     HIS A   183                                                      
REMARK 465     MET A   184                                                      
REMARK 465     ASP A   185                                                      
REMARK 465     ASP A   186                                                      
REMARK 465     PRO A   187                                                      
REMARK 465     GLY A   188                                                      
REMARK 465     GLN A   189                                                      
REMARK 465     GLY A   190                                                      
REMARK 465     ILE A   191                                                      
REMARK 465     GLN A   192                                                      
REMARK 465     GLN A   193                                                      
REMARK 465     GLN A   194                                                      
REMARK 465     HIS A   195                                                      
REMARK 465     SER A   196                                                      
REMARK 465     SER A   197                                                      
REMARK 465     ALA A   198                                                      
REMARK 465     ALA A   199                                                      
REMARK 465     ALA A   200                                                      
REMARK 465     ALA A   201                                                      
REMARK 465     ALA A   202                                                      
REMARK 465     GLN A   203                                                      
REMARK 465     GLN A   204                                                      
REMARK 465     GLY A   205                                                      
REMARK 465     GLY A   206                                                      
REMARK 465     TYR A   207                                                      
REMARK 465     GLU A   208                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS A 312       58.98    -97.88                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 A 501                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 A 502                 
DBREF  5OJ8 A  185   418  UNP    Q07866   KLC1_HUMAN     185    418             
SEQADV 5OJ8 MET A  164  UNP  Q07866              INITIATING METHIONINE          
SEQADV 5OJ8 GLY A  165  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 SER A  166  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 SER A  167  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 HIS A  168  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 HIS A  169  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 HIS A  170  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 HIS A  171  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 HIS A  172  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 HIS A  173  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 SER A  174  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 SER A  175  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 GLY A  176  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 LEU A  177  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 VAL A  178  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 PRO A  179  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 ARG A  180  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 GLY A  181  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 SER A  182  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 HIS A  183  UNP  Q07866              EXPRESSION TAG                 
SEQADV 5OJ8 MET A  184  UNP  Q07866              EXPRESSION TAG                 
SEQRES   1 A  255  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 A  255  LEU VAL PRO ARG GLY SER HIS MET ASP ASP PRO GLY GLN          
SEQRES   3 A  255  GLY ILE GLN GLN GLN HIS SER SER ALA ALA ALA ALA ALA          
SEQRES   4 A  255  GLN GLN GLY GLY TYR GLU ILE PRO ALA ARG LEU ARG THR          
SEQRES   5 A  255  LEU HIS ASN LEU VAL ILE GLN TYR ALA SER GLN GLY ARG          
SEQRES   6 A  255  TYR GLU VAL ALA VAL PRO LEU CYS LYS GLN ALA LEU GLU          
SEQRES   7 A  255  ASP LEU GLU LYS THR SER GLY HIS ASP HIS PRO ASP VAL          
SEQRES   8 A  255  ALA THR MET LEU ASN ILE LEU ALA LEU VAL TYR ARG ASP          
SEQRES   9 A  255  GLN ASN LYS TYR LYS ASP ALA ALA ASN LEU LEU ASN ASP          
SEQRES  10 A  255  ALA LEU ALA ILE ARG GLU LYS THR LEU GLY LYS ASP HIS          
SEQRES  11 A  255  PRO ALA VAL ALA ALA THR LEU ASN ASN LEU ALA VAL LEU          
SEQRES  12 A  255  TYR GLY LYS ARG GLY LYS TYR LYS GLU ALA GLU PRO LEU          
SEQRES  13 A  255  CYS LYS ARG ALA LEU GLU ILE ARG GLU LYS VAL LEU GLY          
SEQRES  14 A  255  LYS ASP HIS PRO ASP VAL ALA LYS GLN LEU ASN ASN LEU          
SEQRES  15 A  255  ALA LEU LEU CYS GLN ASN GLN GLY LYS TYR GLU GLU VAL          
SEQRES  16 A  255  GLU TYR TYR TYR GLN ARG ALA LEU GLU ILE TYR GLN THR          
SEQRES  17 A  255  LYS LEU GLY PRO ASP ASP PRO ASN VAL ALA LYS THR LYS          
SEQRES  18 A  255  ASN ASN LEU ALA SER CYS TYR LEU LYS GLN GLY LYS PHE          
SEQRES  19 A  255  LYS GLN ALA GLU THR LEU TYR LYS GLU ILE LEU THR ARG          
SEQRES  20 A  255  ALA HIS GLU ARG GLU PHE GLY SER                              
HET    PO4  A 501       5                                                       
HET    PO4  A 502       5                                                       
HETNAM     PO4 PHOSPHATE ION                                                    
FORMUL   2  PO4    2(O4 P 3-)                                                   
FORMUL   4  HOH   *2(H2 O)                                                      
HELIX    1 AA1 PRO A  210  GLN A  226  1                                  17    
HELIX    2 AA2 ARG A  228  GLY A  248  1                                  21    
HELIX    3 AA3 HIS A  251  GLN A  268  1                                  18    
HELIX    4 AA4 LYS A  270  GLY A  290  1                                  21    
HELIX    5 AA5 HIS A  293  ARG A  310  1                                  18    
HELIX    6 AA6 LYS A  312  GLY A  332  1                                  21    
HELIX    7 AA7 HIS A  335  ASN A  351  1                                  17    
HELIX    8 AA8 LYS A  354  LEU A  373  1                                  20    
HELIX    9 AA9 ASP A  377  GLY A  395  1                                  19    
HELIX   10 AB1 LYS A  396  PHE A  416  1                                  21    
SITE     1 AC1  2 TYR A 223  ARG A 228                                          
SITE     1 AC2  6 ARG A 266  ASN A 269  TYR A 271  LYS A 309                    
SITE     2 AC2  6 LYS A 393  HIS A 412                                          
CRYST1   69.530   69.530  199.730  90.00  90.00 120.00 P 62 2 2     12          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.014382  0.008304  0.000000        0.00000                         
SCALE2      0.000000  0.016607  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.005007        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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