GenomeNet

Database: PDB
Entry: 5TUU
LinkDB: 5TUU
Original site: 5TUU 
HEADER    TRANSCRIPTION                           07-NOV-16   5TUU              
TITLE     CRYSTAL STRUCTURE OF THE E2F4-DP1 COILED COIL AND MARKED-BOX DOMAINS  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TRANSCRIPTION FACTOR DP1;                                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: UNP RESIDUES 199-350;                                      
COMPND   5 SYNONYM: DRTF1-POLYPEPTIDE 1,DRTF1,E2F DIMERIZATION PARTNER 1;       
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: TRANSCRIPTION FACTOR E2F4;                                 
COMPND   9 CHAIN: B;                                                            
COMPND  10 FRAGMENT: UNP RESIDUES 91-198;                                       
COMPND  11 SYNONYM: E2F-4;                                                      
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: TFDP1, DP1;                                                    
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  10 ORGANISM_COMMON: HUMAN;                                              
SOURCE  11 ORGANISM_TAXID: 9606;                                                
SOURCE  12 GENE: E2F4;                                                          
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 469008                                      
KEYWDS    TRANSCRIPTION FACTOR, CELL-CYCLE REGULATION, COILED COIL DOMIAN,      
KEYWDS   2 TRANSCRIPTION                                                        
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    T.J.LIBAN,S.M.RUBIN,S.M.TRIPATHI                                      
REVDAT   5   04-DEC-19 5TUU    1       REMARK                                   
REVDAT   4   27-SEP-17 5TUU    1       REMARK                                   
REVDAT   3   24-MAY-17 5TUU    1       JRNL                                     
REVDAT   2   10-MAY-17 5TUU    1       JRNL                                     
REVDAT   1   03-MAY-17 5TUU    0                                                
JRNL        AUTH   T.J.LIBAN,E.M.MEDINA,S.TRIPATHI,S.SENGUPTA,R.W.HENRY,        
JRNL        AUTH 2 N.E.BUCHLER,S.M.RUBIN                                        
JRNL        TITL   CONSERVATION AND DIVERGENCE OF C-TERMINAL DOMAIN STRUCTURE   
JRNL        TITL 2 IN THE RETINOBLASTOMA PROTEIN FAMILY.                        
JRNL        REF    PROC. NATL. ACAD. SCI.        V. 114  4942 2017              
JRNL        REF  2 U.S.A.                                                       
JRNL        REFN                   ESSN 1091-6490                               
JRNL        PMID   28439018                                                     
JRNL        DOI    10.1073/PNAS.1619170114                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.25 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.10.1_2155: ???)                            
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.25                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 53.47                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.390                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 94.4                           
REMARK   3   NUMBER OF REFLECTIONS             : 13401                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.207                           
REMARK   3   R VALUE            (WORKING SET) : 0.205                           
REMARK   3   FREE R VALUE                     : 0.239                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.800                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 643                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 53.4808 -  3.8481    0.95     2641   140  0.1766 0.2053        
REMARK   3     2  3.8481 -  3.0544    0.96     2579   144  0.2031 0.2384        
REMARK   3     3  3.0544 -  2.6684    0.94     2524   131  0.2449 0.2966        
REMARK   3     4  2.6684 -  2.4244    0.94     2515   116  0.2540 0.3023        
REMARK   3     5  2.4244 -  2.2506    0.93     2499   112  0.2739 0.3242        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.330            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 28.760           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.005           1967                                  
REMARK   3   ANGLE     :  0.740           2662                                  
REMARK   3   CHIRALITY :  0.043            308                                  
REMARK   3   PLANARITY :  0.006            352                                  
REMARK   3   DIHEDRAL  : 17.460           1218                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 11                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 197 THROUGH 246 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -24.0549  -1.7523   3.4772              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2771 T22:   0.8368                                     
REMARK   3      T33:   0.5168 T12:   0.0966                                     
REMARK   3      T13:  -0.0785 T23:   0.0092                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.5633 L22:   2.0331                                     
REMARK   3      L33:   6.8511 L12:  -0.4135                                     
REMARK   3      L13:  -4.6339 L23:   0.4788                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1499 S12:  -0.4365 S13:  -0.2121                       
REMARK   3      S21:   0.0655 S22:  -0.1974 S23:   0.3690                       
REMARK   3      S31:  -0.2547 S32:   0.0760 S33:   0.2071                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 247 THROUGH 316 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -1.5884  -5.8331 -11.5785              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3457 T22:   0.1873                                     
REMARK   3      T33:   0.2794 T12:  -0.0615                                     
REMARK   3      T13:   0.0033 T23:  -0.0121                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.9352 L22:   3.4081                                     
REMARK   3      L33:   3.4205 L12:  -2.2117                                     
REMARK   3      L13:  -0.2278 L23:  -1.0001                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0914 S12:   0.1895 S13:   0.1219                       
REMARK   3      S21:  -0.2538 S22:  -0.0221 S23:  -0.0672                       
REMARK   3      S31:  -0.2016 S32:  -0.0043 S33:   0.1312                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 317 THROUGH 339 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -24.4198   8.3038  -5.3435              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8336 T22:   0.9058                                     
REMARK   3      T33:   0.8988 T12:   0.4013                                     
REMARK   3      T13:  -0.1129 T23:  -0.1298                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.5922 L22:   3.0883                                     
REMARK   3      L33:   6.2302 L12:  -0.5839                                     
REMARK   3      L13:   0.9145 L23:   0.4568                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0213 S12:  -0.5448 S13:   1.2010                       
REMARK   3      S21:  -0.6596 S22:  -0.1696 S23:   1.5883                       
REMARK   3      S31:  -1.0992 S32:  -1.3569 S33:   0.2060                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 91 THROUGH 96 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -43.8112   5.7042  31.1238              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.2934 T22:   0.6367                                     
REMARK   3      T33:   0.5956 T12:   0.2218                                     
REMARK   3      T13:   0.2857 T23:  -0.1470                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.2421 L22:   2.4459                                     
REMARK   3      L33:   3.9857 L12:   0.7914                                     
REMARK   3      L13:   4.3408 L23:  -0.7540                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2802 S12:   0.5883 S13:  -0.0145                       
REMARK   3      S21:  -1.2896 S22:  -0.0671 S23:  -1.1640                       
REMARK   3      S31:   1.1448 S32:   0.5738 S33:  -1.1731                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 97 THROUGH 128 )                  
REMARK   3    ORIGIN FOR THE GROUP (A): -32.7665  -3.9780   4.9190              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3720 T22:   1.0683                                     
REMARK   3      T33:   0.6793 T12:   0.0259                                     
REMARK   3      T13:  -0.0442 T23:  -0.0903                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.7514 L22:   1.0899                                     
REMARK   3      L33:   6.5763 L12:  -0.6455                                     
REMARK   3      L13:  -3.9910 L23:  -0.2774                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0083 S12:   0.6123 S13:  -0.6882                       
REMARK   3      S21:  -0.0117 S22:  -0.3735 S23:   0.5016                       
REMARK   3      S31:  -0.3825 S32:  -1.2876 S33:   0.2907                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 129 THROUGH 141 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -17.0068  -5.9521 -20.7360              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6354 T22:   1.0249                                     
REMARK   3      T33:   0.4727 T12:  -0.0371                                     
REMARK   3      T13:  -0.1102 T23:  -0.1628                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.6556 L22:   4.1951                                     
REMARK   3      L33:   2.7257 L12:  -0.5575                                     
REMARK   3      L13:  -1.4270 L23:  -0.8018                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1615 S12:   0.3009 S13:  -1.0360                       
REMARK   3      S21:  -0.6123 S22:  -0.6404 S23:   0.4751                       
REMARK   3      S31:   0.4521 S32:  -1.9869 S33:   0.0473                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 142 THROUGH 148 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -1.2993   2.8129 -15.3602              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5206 T22:   0.3251                                     
REMARK   3      T33:   0.5330 T12:  -0.0186                                     
REMARK   3      T13:   0.0865 T23:   0.0202                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   9.4441 L22:   8.2576                                     
REMARK   3      L33:   4.5482 L12:   3.1864                                     
REMARK   3      L13:   5.1540 L23:  -1.3007                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1918 S12:  -0.1479 S13:   0.9244                       
REMARK   3      S21:  -0.1624 S22:  -0.1325 S23:  -0.7971                       
REMARK   3      S31:  -0.5424 S32:   0.1811 S33:  -0.0890                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 149 THROUGH 166 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -0.5411 -10.7578  -9.7139              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4237 T22:   0.2666                                     
REMARK   3      T33:   0.3642 T12:  -0.0288                                     
REMARK   3      T13:   0.0194 T23:   0.0241                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.6457 L22:   4.4323                                     
REMARK   3      L33:   1.6635 L12:  -1.6012                                     
REMARK   3      L13:  -0.7039 L23:   0.1229                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.4137 S12:   0.1985 S13:  -0.0943                       
REMARK   3      S21:  -0.4033 S22:   0.5286 S23:  -0.1888                       
REMARK   3      S31:   0.0901 S32:   0.0632 S33:   0.1353                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 167 THROUGH 181 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  11.9616 -13.4869   3.6200              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4664 T22:   0.5287                                     
REMARK   3      T33:   0.4172 T12:  -0.0005                                     
REMARK   3      T13:  -0.0300 T23:   0.0336                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.3767 L22:   2.9468                                     
REMARK   3      L33:   5.0742 L12:   0.1915                                     
REMARK   3      L13:   1.1782 L23:   1.2391                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2677 S12:   0.0369 S13:   0.3122                       
REMARK   3      S21:   0.4884 S22:  -0.0334 S23:  -0.5822                       
REMARK   3      S31:   0.5637 S32:   0.9245 S33:   0.0474                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 182 THROUGH 190 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -2.1224 -22.6720 -11.0941              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5206 T22:   0.3730                                     
REMARK   3      T33:   0.5071 T12:  -0.0233                                     
REMARK   3      T13:   0.1849 T23:  -0.0024                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.5256 L22:   2.5761                                     
REMARK   3      L33:   5.6131 L12:   0.5898                                     
REMARK   3      L13:   2.2457 L23:   0.1274                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3313 S12:  -0.1800 S13:  -0.8294                       
REMARK   3      S21:   0.1396 S22:  -0.1993 S23:   0.1907                       
REMARK   3      S31:   0.7555 S32:   0.0693 S33:  -0.0212                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 191 THROUGH 196 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):   2.8972  -8.7389 -17.2773              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5020 T22:   0.4461                                     
REMARK   3      T33:   0.3541 T12:  -0.0770                                     
REMARK   3      T13:  -0.0080 T23:  -0.0072                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.2213 L22:   3.4825                                     
REMARK   3      L33:   7.0432 L12:   3.3661                                     
REMARK   3      L13:  -3.9571 L23:  -4.2075                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.6667 S12:   0.6778 S13:   0.3384                       
REMARK   3      S21:  -0.2052 S22:   0.1806 S23:  -0.1685                       
REMARK   3      S31:  -0.9239 S32:   1.0103 S33:   0.1565                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5TUU COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 08-NOV-16.                  
REMARK 100 THE DEPOSITION ID IS D_1000223819.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 19-FEB-15                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 23-ID-B                            
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.03320                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 300 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : IMOSFLM                            
REMARK 200  DATA SCALING SOFTWARE          : SCALA                              
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 13407                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.250                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 53.960                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 94.0                               
REMARK 200  DATA REDUNDANCY                : 3.500                              
REMARK 200  R MERGE                    (I) : 0.05600                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 20.3000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.25                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.32                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 93.6                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 2.40                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.31000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.900                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: PDB 2AZE                                             
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 49.74                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.45                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M MES PH 6.5, 15% PEG 6000, VAPOR     
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 291K                           
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: I 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z+1/2                                       
REMARK 290       4555   -X+1/2,Y+1/2,-Z+1/2                                     
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       24.01076            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       18.77500            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       53.46556            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       24.01076            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       18.77500            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000       53.46556            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 6150 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 14950 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -48.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 16040 ANGSTROM**2                         
REMARK 350 SURFACE AREA OF THE COMPLEX: 26150 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -118.0 KCAL/MOL                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375      HOH A 513  LIES ON A SPECIAL POSITION.                          
REMARK 375      HOH A 523  LIES ON A SPECIAL POSITION.                          
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A   340                                                      
REMARK 465     THR A   341                                                      
REMARK 465     VAL A   342                                                      
REMARK 465     GLY A   343                                                      
REMARK 465     GLY A   344                                                      
REMARK 465     VAL A   345                                                      
REMARK 465     PHE A   346                                                      
REMARK 465     ILE A   347                                                      
REMARK 465     THR A   348                                                      
REMARK 465     THR A   349                                                      
REMARK 465     ALA A   350                                                      
REMARK 465     GLY B    88                                                      
REMARK 465     HIS B    89                                                      
REMARK 465     MET B    90                                                      
REMARK 465     LYS B   197                                                      
REMARK 465     GLU B   198                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLN A 210    OE1  NE2                                            
REMARK 470     LYS A 217    CG   CD   CE   NZ                                   
REMARK 470     GLU A 317    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 320    CG   CD   CE   NZ                                   
REMARK 470     ARG A 323    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS A 328    CG   CD   CE   NZ                                   
REMARK 470     GLU A 331    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 336    CG   CD   OE1  OE2                                  
REMARK 470     LEU B 100    CG   CD1  CD2                                       
REMARK 470     LYS B 101    CG   CD   CE   NZ                                   
REMARK 470     GLU B 105    CG   CD   OE1  OE2                                  
REMARK 470     GLN B 108    OE1  NE2                                            
REMARK 470     GLN B 109    CG   CD   OE1  NE2                                  
REMARK 470     ARG B 110    NE   CZ   NH1  NH2                                  
REMARK 470     LYS B 118    CG   CD   CE   NZ                                   
REMARK 470     GLN B 123    CG   CD   OE1  NE2                                  
REMARK 470     GLU B 143    CG   CD   OE1  OE2                                  
REMARK 470     ASN B 174    CG   OD1  ND2                                       
REMARK 470     LYS B 178    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ARG A 249      148.54    177.93                                   
REMARK 500    ASN A 254     -162.47    -75.19                                   
REMARK 500    ALA B  94     -110.77     57.58                                   
REMARK 500    ASP B  95       38.92     35.61                                   
REMARK 500    THR B 129      -72.07    -80.32                                   
REMARK 500    ASN B 174       -4.70     88.98                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL A 401                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL B 201                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5TUV   RELATED DB: PDB                                   
DBREF  5TUU A  199   350  UNP    Q14186   TFDP1_HUMAN    199    350             
DBREF  5TUU B   91   198  UNP    Q16254   E2F4_HUMAN      91    198             
SEQADV 5TUU GLY A  196  UNP  Q14186              EXPRESSION TAG                 
SEQADV 5TUU GLU A  197  UNP  Q14186              EXPRESSION TAG                 
SEQADV 5TUU PHE A  198  UNP  Q14186              EXPRESSION TAG                 
SEQADV 5TUU GLY B   88  UNP  Q16254              EXPRESSION TAG                 
SEQADV 5TUU HIS B   89  UNP  Q16254              EXPRESSION TAG                 
SEQADV 5TUU MET B   90  UNP  Q16254              EXPRESSION TAG                 
SEQRES   1 A  155  GLY GLU PHE ALA GLN GLU CYS GLN ASN LEU GLU VAL GLU          
SEQRES   2 A  155  ARG GLN ARG ARG LEU GLU ARG ILE LYS GLN LYS GLN SER          
SEQRES   3 A  155  GLN LEU GLN GLU LEU ILE LEU GLN GLN ILE ALA PHE LYS          
SEQRES   4 A  155  ASN LEU VAL GLN ARG ASN ARG HIS ALA GLU GLN GLN ALA          
SEQRES   5 A  155  SER ARG PRO PRO PRO PRO ASN SER VAL ILE HIS LEU PRO          
SEQRES   6 A  155  PHE ILE ILE VAL ASN THR SER LYS LYS THR VAL ILE ASP          
SEQRES   7 A  155  CYS SER ILE SER ASN ASP LYS PHE GLU TYR LEU PHE ASN          
SEQRES   8 A  155  PHE ASP ASN THR PHE GLU ILE HIS ASP ASP ILE GLU VAL          
SEQRES   9 A  155  LEU LYS ARG MET GLY MET ALA CYS GLY LEU GLU SER GLY          
SEQRES  10 A  155  SER CYS SER ALA GLU ASP LEU LYS MET ALA ARG SER LEU          
SEQRES  11 A  155  VAL PRO LYS ALA LEU GLU PRO TYR VAL THR GLU MET ALA          
SEQRES  12 A  155  GLN GLY THR VAL GLY GLY VAL PHE ILE THR THR ALA              
SEQRES   1 B  111  GLY HIS MET ARG GLU ILE ALA ASP LYS LEU ILE GLU LEU          
SEQRES   2 B  111  LYS ALA GLU ILE GLU GLU LEU GLN GLN ARG GLU GLN GLU          
SEQRES   3 B  111  LEU ASP GLN HIS LYS VAL TRP VAL GLN GLN SER ILE ARG          
SEQRES   4 B  111  ASN VAL THR GLU ASP VAL GLN ASN SER CYS LEU ALA TYR          
SEQRES   5 B  111  VAL THR HIS GLU ASP ILE CYS ARG CYS PHE ALA GLY ASP          
SEQRES   6 B  111  THR LEU LEU ALA ILE ARG ALA PRO SER GLY THR SER LEU          
SEQRES   7 B  111  GLU VAL PRO ILE PRO GLU GLY LEU ASN GLY GLN LYS LYS          
SEQRES   8 B  111  TYR GLN ILE HIS LEU LYS SER VAL SER GLY PRO ILE GLU          
SEQRES   9 B  111  VAL LEU LEU VAL ASN LYS GLU                                  
HET    GOL  A 401       6                                                       
HET    GOL  B 201       6                                                       
HETNAM     GOL GLYCEROL                                                         
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL                                    
FORMUL   3  GOL    2(C3 H8 O3)                                                  
FORMUL   5  HOH   *42(H2 O)                                                     
HELIX    1 AA1 GLY A  196  SER A  248  1                                  53    
HELIX    2 AA2 ASP A  296  GLY A  304  1                                   9    
HELIX    3 AA3 GLY A  308  GLY A  312  5                                   5    
HELIX    4 AA4 SER A  315  LEU A  325  1                                  11    
HELIX    5 AA5 VAL A  326  ALA A  329  5                                   4    
HELIX    6 AA6 LEU A  330  ALA A  338  1                                   9    
HELIX    7 AA7 ASP B   95  GLU B  130  1                                  36    
HELIX    8 AA8 ASP B  131  SER B  135  5                                   5    
HELIX    9 AA9 HIS B  142  PHE B  149  1                                   8    
SHEET    1 AA1 2 VAL A 256  HIS A 258  0                                        
SHEET    2 AA1 2 TYR B 139  THR B 141 -1  O  VAL B 140   N  ILE A 257           
SHEET    1 AA2 4 PHE A 291  ASP A 295  0                                        
SHEET    2 AA2 4 ILE A 262  SER A 267 -1  N  ILE A 263   O  HIS A 294           
SHEET    3 AA2 4 ASP B 152  ARG B 158 -1  O  LEU B 155   N  VAL A 264           
SHEET    4 AA2 4 GLU B 191  LEU B 194 -1  O  GLU B 191   N  ARG B 158           
SHEET    1 AA3 4 ILE A 272  ILE A 276  0                                        
SHEET    2 AA3 4 GLU A 282  PHE A 287 -1  O  ASN A 286   N  ASP A 273           
SHEET    3 AA3 4 TYR B 179  LYS B 184 -1  O  TYR B 179   N  PHE A 287           
SHEET    4 AA3 4 SER B 164  GLU B 166 -1  N  GLU B 166   O  HIS B 182           
CISPEP   1 LEU A  259    PRO A  260          0        -2.14                     
SITE     1 AC1  5 ASP A 273  CYS A 274  HOH A 501  HOH A 505                    
SITE     2 AC1  5 GOL B 201                                                     
SITE     1 AC2  2 ILE A 272  GOL A 401                                          
CRYST1   73.575   37.550  109.942  90.00 103.44  90.00 I 1 2 1       4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.013592  0.000000  0.003248        0.00000                         
SCALE2      0.000000  0.026631  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.009352        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system