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Database: PDB
Entry: 5VPA
LinkDB: 5VPA
Original site: 5VPA 
HEADER    TRANSCRIPTION                           04-MAY-17   5VPA              
TITLE     TRANSCRIPTION FACTOR FOSB/JUND BZIP DOMAIN                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN FOSB;                                              
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: UNP RESIDUES 153-219;                                      
COMPND   5 SYNONYM: G0/G1 SWITCH REGULATORY PROTEIN 3;                          
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: TRANSCRIPTION FACTOR JUN-D;                                
COMPND   9 CHAIN: B;                                                            
COMPND  10 FRAGMENT: UNP RESIDUES 266-332;                                      
COMPND  11 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: FOSB, G0S3;                                                    
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: ROSETTA 2 (DE3);                           
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET21-NESG;                               
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  13 ORGANISM_COMMON: HUMAN;                                              
SOURCE  14 ORGANISM_TAXID: 9606;                                                
SOURCE  15 GENE: JUND;                                                          
SOURCE  16 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  17 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  18 EXPRESSION_SYSTEM_STRAIN: ROSETTA 2 (DE3);                           
SOURCE  19 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  20 EXPRESSION_SYSTEM_PLASMID: PET21A                                    
KEYWDS    ACTIVATOR PROTEIN-1, BASIC LEUCINE ZIPPER, BZIP, FOS, JUN,            
KEYWDS   2 TRANSCRIPTION FACTOR, DNA-BINDING PROTEIN, REDOX SWITCH, COILED-     
KEYWDS   3 COIL, TRANSCRIPTION                                                  
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Z.YIN,M.MACHIUS,G.RUDENKO                                             
REVDAT   4   11-DEC-19 5VPA    1       REMARK                                   
REVDAT   3   15-NOV-17 5VPA    1       JRNL                                     
REVDAT   2   18-OCT-17 5VPA    1       JRNL                                     
REVDAT   1   06-SEP-17 5VPA    0                                                
JRNL        AUTH   Z.YIN,M.MACHIUS,E.J.NESTLER,G.RUDENKO                        
JRNL        TITL   ACTIVATOR PROTEIN-1: REDOX SWITCH CONTROLLING STRUCTURE AND  
JRNL        TITL 2 DNA-BINDING.                                                 
JRNL        REF    NUCLEIC ACIDS RES.            V.  45 11425 2017              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   28981703                                                     
JRNL        DOI    10.1093/NAR/GKX795                                           
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.83 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.11.1_2575                                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.83                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 34.20                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 71.5                           
REMARK   3   NUMBER OF REFLECTIONS             : 4417                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.248                           
REMARK   3   R VALUE            (WORKING SET) : 0.245                           
REMARK   3   FREE R VALUE                     : 0.283                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 9.800                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 433                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 34.2069 -  4.0796    1.00     1911   212  0.2359 0.2746        
REMARK   3     2  4.0796 -  3.2389    0.74     1374   145  0.2493 0.2784        
REMARK   3     3  3.2389 -  2.8297    0.39      699    76  0.2715 0.3372        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.380            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 31.570           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 32.12                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 66.46                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.006            903                                  
REMARK   3   ANGLE     :  0.685           1209                                  
REMARK   3   CHIRALITY :  0.029            144                                  
REMARK   3   PLANARITY :  0.003            158                                  
REMARK   3   DIHEDRAL  : 13.159            599                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 3                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 164 THROUGH 177 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):   3.9541   9.4427 285.4320              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3855 T22:   0.3770                                     
REMARK   3      T33:   0.7889 T12:  -0.1781                                     
REMARK   3      T13:  -0.0961 T23:  -0.0737                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.8059 L22:   4.4498                                     
REMARK   3      L33:   1.8038 L12:   1.2732                                     
REMARK   3      L13:  -0.6482 L23:   0.8579                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.5291 S12:   0.0036 S13:  -0.8353                       
REMARK   3      S21:   0.4338 S22:   0.1641 S23:   0.0769                       
REMARK   3      S31:   0.8482 S32:  -0.3690 S33:  -0.5223                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 178 THROUGH 217 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -2.1218  27.1751 317.0291              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0925 T22:   0.4611                                     
REMARK   3      T33:   0.1230 T12:   0.0763                                     
REMARK   3      T13:  -0.0769 T23:  -0.1044                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.7939 L22:   4.7493                                     
REMARK   3      L33:   6.6989 L12:  -1.8837                                     
REMARK   3      L13:  -2.6394 L23:   4.4682                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0906 S12:   0.1695 S13:  -0.1182                       
REMARK   3      S21:   0.2003 S22:  -0.0747 S23:   0.3608                       
REMARK   3      S31:  -0.0351 S32:  -0.2975 S33:   0.2193                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 275 THROUGH 332 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):   6.7691  24.5930 308.7369              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0746 T22:   0.4294                                     
REMARK   3      T33:  -0.0568 T12:  -0.0566                                     
REMARK   3      T13:   0.0941 T23:  -0.0305                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.1253 L22:   1.0285                                     
REMARK   3      L33:   2.6817 L12:  -0.3911                                     
REMARK   3      L13:  -1.4563 L23:   1.1200                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0807 S12:   0.1284 S13:  -0.0127                       
REMARK   3      S21:  -0.1053 S22:   0.0882 S23:  -0.0019                       
REMARK   3      S31:  -0.1131 S32:   0.1051 S33:  -0.2418                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5VPA COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 05-MAY-17.                  
REMARK 100 THE DEPOSITION ID IS D_1000227524.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 13-NOV-16                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 8.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 17-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.999                              
REMARK 200  MONOCHROMATOR                  : SI(111)                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS3 S 6M              
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 6010                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.830                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 34.204                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY                : 9.500                              
REMARK 200  R MERGE                    (I) : 0.09600                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 25.0000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.83                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.85                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 9.50                               
REMARK 200  R MERGE FOR SHELL          (I) : 1.16700                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.200                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 1FOS                                                 
REMARK 200                                                                      
REMARK 200 REMARK: THIN PLATES                                                  
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 67.52                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.79                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 3 M 1,6-HEXANEDIOL, 50 MM TRIS PH 8.5    
REMARK 280  AND 5 MM MGCL2, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: I 2 2 2                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z                                                 
REMARK 290       3555   -X,Y,-Z                                                 
REMARK 290       4555   X,-Y,-Z                                                 
REMARK 290       5555   X+1/2,Y+1/2,Z+1/2                                       
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2                                     
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2                                     
REMARK 290       8555   X+1/2,-Y+1/2,-Z+1/2                                     
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       26.30250            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       47.67600            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000       49.10000            
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       26.30250            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       47.67600            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       49.10000            
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       26.30250            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       47.67600            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       49.10000            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       26.30250            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       47.67600            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       49.10000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 2610 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 8890 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -35.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER A   152                                                      
REMARK 465     GLU A   153                                                      
REMARK 465     GLU A   154                                                      
REMARK 465     GLU A   155                                                      
REMARK 465     GLU A   156                                                      
REMARK 465     LYS A   157                                                      
REMARK 465     ARG A   158                                                      
REMARK 465     ARG A   159                                                      
REMARK 465     VAL A   160                                                      
REMARK 465     ARG A   161                                                      
REMARK 465     ARG A   162                                                      
REMARK 465     GLU A   163                                                      
REMARK 465     HIS A   218                                                      
REMARK 465     LYS A   219                                                      
REMARK 465     SER B   265                                                      
REMARK 465     GLN B   266                                                      
REMARK 465     GLU B   267                                                      
REMARK 465     ARG B   268                                                      
REMARK 465     ILE B   269                                                      
REMARK 465     LYS B   270                                                      
REMARK 465     ALA B   271                                                      
REMARK 465     GLU B   272                                                      
REMARK 465     ARG B   273                                                      
REMARK 465     LYS B   274                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LEU A 167    CG   CD1  CD2                                       
REMARK 470     LYS A 171    CG   CD   CE   NZ                                   
REMARK 470     ARG A 175    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 186    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 203    CG   CD   OE1  OE2                                  
REMARK 470     ARG B 275    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG B 277    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS B 284    CG   CD   CE   NZ                                   
REMARK 470     VAL B 332    CG1  CG2                                            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN A 165       26.02   -143.12                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue NA B 401                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL B 402                  
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5VPB   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPC   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPD   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPE   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPF   RELATED DB: PDB                                   
DBREF  5VPA A  153   219  UNP    P53539   FOSB_HUMAN     153    219             
DBREF  5VPA B  266   332  UNP    P17535   JUND_HUMAN     266    332             
SEQADV 5VPA SER A  152  UNP  P53539              EXPRESSION TAG                 
SEQADV 5VPA SER B  265  UNP  P17535              EXPRESSION TAG                 
SEQRES   1 A   68  SER GLU GLU GLU GLU LYS ARG ARG VAL ARG ARG GLU ARG          
SEQRES   2 A   68  ASN LYS LEU ALA ALA ALA LYS CYS ARG ASN ARG ARG ARG          
SEQRES   3 A   68  GLU LEU THR ASP ARG LEU GLN ALA GLU THR ASP GLN LEU          
SEQRES   4 A   68  GLU GLU GLU LYS ALA GLU LEU GLU SER GLU ILE ALA GLU          
SEQRES   5 A   68  LEU GLN LYS GLU LYS GLU ARG LEU GLU PHE VAL LEU VAL          
SEQRES   6 A   68  ALA HIS LYS                                                  
SEQRES   1 B   68  SER GLN GLU ARG ILE LYS ALA GLU ARG LYS ARG LEU ARG          
SEQRES   2 B   68  ASN ARG ILE ALA ALA SER LYS CYS ARG LYS ARG LYS LEU          
SEQRES   3 B   68  GLU ARG ILE SER ARG LEU GLU GLU LYS VAL LYS THR LEU          
SEQRES   4 B   68  LYS SER GLN ASN THR GLU LEU ALA SER THR ALA SER LEU          
SEQRES   5 B   68  LEU ARG GLU GLN VAL ALA GLN LEU LYS GLN LYS VAL LEU          
SEQRES   6 B   68  SER HIS VAL                                                  
HET     NA  B 401       1                                                       
HET     CL  B 402       1                                                       
HETNAM      NA SODIUM ION                                                       
HETNAM      CL CHLORIDE ION                                                     
FORMUL   3   NA    NA 1+                                                        
FORMUL   4   CL    CL 1-                                                        
FORMUL   5  HOH   *5(H2 O)                                                      
HELIX    1 AA1 ASN A  165  ARG A  175  1                                  11    
HELIX    2 AA2 ARG A  177  VAL A  216  1                                  40    
HELIX    3 AA3 ASN B  278  SER B  330  1                                  53    
SSBOND   1 CYS A  172    CYS B  285                          1555   1555  2.03  
SITE     1 AC1  1 GLU B 309                                                     
SITE     1 AC2  1 ARG B 286                                                     
CRYST1   52.605   95.352   98.200  90.00  90.00  90.00 I 2 2 2       8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.019010  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.010487  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.010183        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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