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Database: PDB
Entry: 5VPB
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Original site: 5VPB 
HEADER    TRANSCRIPTION                           04-MAY-17   5VPB              
TITLE     TRANSCRIPTION FACTOR FOSB/JUND BZIP DOMAIN IN ITS OXIDIZED FORM, TYPE-
TITLE    2 I CRYSTAL                                                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN FOSB;                                              
COMPND   3 CHAIN: C, A;                                                         
COMPND   4 FRAGMENT: UNP RESIDUES 153-219;                                      
COMPND   5 SYNONYM: G0/G1 SWITCH REGULATORY PROTEIN 3;                          
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: TRANSCRIPTION FACTOR JUN-D;                                
COMPND   9 CHAIN: D, B;                                                         
COMPND  10 FRAGMENT: UNP RESIDUES 266-332;                                      
COMPND  11 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: FOSB, G0S3;                                                    
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: ROSETTA 2 (DE3);                           
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET21-NESG;                               
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  13 ORGANISM_COMMON: HUMAN;                                              
SOURCE  14 ORGANISM_TAXID: 9606;                                                
SOURCE  15 GENE: JUND;                                                          
SOURCE  16 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  17 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  18 EXPRESSION_SYSTEM_STRAIN: ROSETTA 2 (DE3);                           
SOURCE  19 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  20 EXPRESSION_SYSTEM_PLASMID: PET21A                                    
KEYWDS    ACTIVATOR PROTEIN-1, BASIC LEUCINE ZIPPER, BZIP, FOS, JUN,            
KEYWDS   2 TRANSCRIPTION FACTOR, DNA-BINDING PROTEIN, REDOX SWITCH, COILED-     
KEYWDS   3 COIL, TRANSCRIPTION                                                  
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Z.YIN,M.MACHIUS,G.RUDENKO                                             
REVDAT   4   11-DEC-19 5VPB    1       REMARK                                   
REVDAT   3   15-NOV-17 5VPB    1       JRNL                                     
REVDAT   2   18-OCT-17 5VPB    1       JRNL                                     
REVDAT   1   06-SEP-17 5VPB    0                                                
JRNL        AUTH   Z.YIN,M.MACHIUS,E.J.NESTLER,G.RUDENKO                        
JRNL        TITL   ACTIVATOR PROTEIN-1: REDOX SWITCH CONTROLLING STRUCTURE AND  
JRNL        TITL 2 DNA-BINDING.                                                 
JRNL        REF    NUCLEIC ACIDS RES.            V.  45 11425 2017              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   28981703                                                     
JRNL        DOI    10.1093/NAR/GKX795                                           
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.69 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.11.1_2575                                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.69                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 43.74                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.400                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 85.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 10200                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.242                           
REMARK   3   R VALUE            (WORKING SET) : 0.238                           
REMARK   3   FREE R VALUE                     : 0.275                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 10.010                          
REMARK   3   FREE R VALUE TEST SET COUNT      : 1021                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 43.7437 -  5.1459    0.99     1537   172  0.2405 0.2504        
REMARK   3     2  5.1459 -  4.0854    1.00     1536   171  0.2030 0.2156        
REMARK   3     3  4.0854 -  3.5693    1.00     1499   166  0.1974 0.2601        
REMARK   3     4  3.5693 -  3.2431    1.00     1538   171  0.2675 0.3507        
REMARK   3     5  3.2431 -  3.0107    0.91     1386   154  0.2765 0.2973        
REMARK   3     6  3.0107 -  2.8332    0.68     1036   115  0.2890 0.3333        
REMARK   3     7  2.8332 -  2.6913    0.42      647    72  0.3193 0.4435        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.380            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 31.140           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 35.88                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 53.77                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.002           1920                                  
REMARK   3   ANGLE     :  0.419           2569                                  
REMARK   3   CHIRALITY :  0.024            309                                  
REMARK   3   PLANARITY :  0.002            336                                  
REMARK   3   DIHEDRAL  :  8.918           1268                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 6                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 163 THROUGH 176 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): 267.3949 -21.3058  57.7488              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5333 T22:   0.7875                                     
REMARK   3      T33:   0.5486 T12:  -0.2325                                     
REMARK   3      T13:   0.0533 T23:   0.0609                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   9.5545 L22:   3.5895                                     
REMARK   3      L33:   8.2698 L12:  -5.1007                                     
REMARK   3      L13:  -2.9150 L23:   4.0840                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.6000 S12:   0.6072 S13:  -0.4393                       
REMARK   3      S21:   0.3478 S22:  -0.1085 S23:  -0.1128                       
REMARK   3      S31:   0.3979 S32:  -0.5955 S33:   0.7002                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 177 THROUGH 216 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): 284.8678   9.6824  58.5119              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3380 T22:   0.1427                                     
REMARK   3      T33:   0.1221 T12:   0.0365                                     
REMARK   3      T13:   0.1162 T23:  -0.0410                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.7442 L22:   7.1947                                     
REMARK   3      L33:   0.1978 L12:   6.3495                                     
REMARK   3      L13:  -0.1366 L23:   0.2496                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3270 S12:   0.3013 S13:   0.2368                       
REMARK   3      S21:  -0.5662 S22:   0.4407 S23:  -0.0899                       
REMARK   3      S31:  -0.0904 S32:   0.1127 S33:  -0.0648                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 267 THROUGH 332 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): 277.8380  -1.9519  65.2436              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1257 T22:   0.0554                                     
REMARK   3      T33:   0.0429 T12:  -0.0465                                     
REMARK   3      T13:  -0.0446 T23:  -0.0181                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.0516 L22:   3.7250                                     
REMARK   3      L33:   0.3201 L12:   1.5960                                     
REMARK   3      L13:  -0.1707 L23:  -0.7372                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0399 S12:   0.2154 S13:  -0.0807                       
REMARK   3      S21:   0.0790 S22:   0.1450 S23:  -0.1000                       
REMARK   3      S31:   0.0233 S32:   0.0120 S33:   0.0286                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 161 THROUGH 176 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): 270.1317  -0.0781  52.0439              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2752 T22:   0.5742                                     
REMARK   3      T33:   0.4590 T12:   0.1983                                     
REMARK   3      T13:  -0.0663 T23:   0.0991                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.9142 L22:   6.4010                                     
REMARK   3      L33:   3.4917 L12:  -2.6287                                     
REMARK   3      L13:   0.1818 L23:   1.0913                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3043 S12:  -0.3436 S13:   0.5019                       
REMARK   3      S21:  -0.0864 S22:   0.0419 S23:   0.7805                       
REMARK   3      S31:  -0.4135 S32:  -0.3423 S33:   0.3002                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 177 THROUGH 219 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): 283.8877 -34.8018  62.4024              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3124 T22:   0.1200                                     
REMARK   3      T33:   0.1455 T12:   0.0925                                     
REMARK   3      T13:   0.1762 T23:   0.0202                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.5128 L22:   0.8608                                     
REMARK   3      L33:   0.4601 L12:  -0.5362                                     
REMARK   3      L13:   0.1589 L23:  -0.4482                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0438 S12:   0.0047 S13:   0.1014                       
REMARK   3      S21:   0.0634 S22:   0.0997 S23:   0.0692                       
REMARK   3      S31:  -0.2784 S32:  -0.1147 S33:   0.1383                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 266 THROUGH 332 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): 282.6181 -21.7181  52.0359              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1264 T22:   0.1139                                     
REMARK   3      T33:   0.1602 T12:   0.0834                                     
REMARK   3      T13:   0.1053 T23:   0.1799                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.3031 L22:   2.1986                                     
REMARK   3      L33:   0.6209 L12:  -0.5503                                     
REMARK   3      L13:   0.3806 L23:  -0.9945                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0252 S12:   0.0015 S13:   0.1087                       
REMARK   3      S21:   0.0369 S22:   0.0008 S23:  -0.0003                       
REMARK   3      S31:  -0.0643 S32:  -0.0469 S33:  -0.0175                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : 2                                           
REMARK   3   NCS GROUP : 1                                                      
REMARK   3    NCS OPERATOR : 1                                                  
REMARK   3     REFERENCE SELECTION: (CHAIN B AND (RESID 267 THROUGH 276 OR      
REMARK   3                          (RESID 277 AND (NAME N OR NAME CA OR NAME   
REMARK   3                          C OR NAME O OR NAME CB )) OR RESID 278      
REMARK   3                          THROUGH 326 OR (RESID 327 AND (NAME N OR    
REMARK   3                          NAME CA OR NAME C OR NAME O OR NAME CB ))   
REMARK   3                          OR RESID 328 THROUGH 332))                  
REMARK   3     SELECTION          : (CHAIN D AND ((RESID 267 THROUGH 274 AND    
REMARK   3                          (NAME N OR NAME CA OR NAME C OR NAME O OR   
REMARK   3                          NAME CB )) OR RESID 275 THROUGH 332))       
REMARK   3     ATOM PAIRS NUMBER  : 741                                         
REMARK   3     RMSD               : NULL                                        
REMARK   3   NCS GROUP : 2                                                      
REMARK   3    NCS OPERATOR : 1                                                  
REMARK   3     REFERENCE SELECTION: (CHAIN A AND (RESID 163 THROUGH 166 OR      
REMARK   3                          (RESID 167 THROUGH 171 AND (NAME N OR       
REMARK   3                          NAME CA OR NAME C OR NAME O OR NAME CB ))   
REMARK   3                          OR RESID 172 THROUGH 204 OR RESID 206 OR    
REMARK   3                          RESID 208 THROUGH 212 OR (RESID 213 AND     
REMARK   3                          (NAME N OR NAME CA OR NAME C OR NAME O OR   
REMARK   3                          NAME CB )) OR RESID 214 THROUGH 216))       
REMARK   3     SELECTION          : (CHAIN C AND (RESID 163 THROUGH 165 OR      
REMARK   3                          (RESID 166 THROUGH 171 AND (NAME N OR       
REMARK   3                          NAME CA OR NAME C OR NAME O OR NAME CB ))   
REMARK   3                          OR RESID 172 THROUGH 174 OR (RESID 175      
REMARK   3                          AND (NAME N OR NAME CA OR NAME C OR NAME    
REMARK   3                          O OR NAME CB )) OR RESID 176 THROUGH 204    
REMARK   3                          OR RESID 206 OR RESID 208 THROUGH 216))     
REMARK   3     ATOM PAIRS NUMBER  : 544                                         
REMARK   3     RMSD               : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5VPB COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 05-MAY-17.                  
REMARK 100 THE DEPOSITION ID IS D_1000227761.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 22-OCT-16                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 7.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 21-ID-F                            
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9787                             
REMARK 200  MONOCHROMATOR                  : DIAMOND [111]                      
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 225 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 11872                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.691                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 43.740                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 3.800                              
REMARK 200  R MERGE                    (I) : 0.06400                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 20.0000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.69                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.75                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 3.80                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.64100                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.000                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 5VPA                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 63.08                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.33                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 45% (V/V) 2-METHYL-2,4-PENTANEDIOL       
REMARK 280  (MPD), 0.3 M NACL AND 0.1 M HEPES PH 7.5, VAPOR DIFFUSION,          
REMARK 280  SITTING DROP, TEMPERATURE 293K                                      
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       46.41250            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       49.63050            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       46.41250            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       49.63050            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 3430 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 8990 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -68.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 3700 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 9380 ANGSTROM**2                        
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -90.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER C   152                                                      
REMARK 465     GLU C   153                                                      
REMARK 465     GLU C   154                                                      
REMARK 465     GLU C   155                                                      
REMARK 465     GLU C   156                                                      
REMARK 465     LYS C   157                                                      
REMARK 465     ARG C   158                                                      
REMARK 465     ARG C   159                                                      
REMARK 465     VAL C   160                                                      
REMARK 465     ARG C   161                                                      
REMARK 465     ARG C   162                                                      
REMARK 465     ALA C   217                                                      
REMARK 465     HIS C   218                                                      
REMARK 465     LYS C   219                                                      
REMARK 465     SER D   265                                                      
REMARK 465     GLN D   266                                                      
REMARK 465     SER A   152                                                      
REMARK 465     GLU A   153                                                      
REMARK 465     GLU A   154                                                      
REMARK 465     GLU A   155                                                      
REMARK 465     GLU A   156                                                      
REMARK 465     LYS A   157                                                      
REMARK 465     ARG A   158                                                      
REMARK 465     ARG A   159                                                      
REMARK 465     VAL A   160                                                      
REMARK 465     SER B   265                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLU C 163    CG   CD   OE1  OE2                                  
REMARK 470     ARG C 164    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LEU C 167    CG   CD1  CD2                                       
REMARK 470     LYS C 171    CG   CD   CE   NZ                                   
REMARK 470     LYS C 206    CG   CD   CE   NZ                                   
REMARK 470     GLU C 209    CG   CD   OE1  OE2                                  
REMARK 470     PHE C 213    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     GLU D 267    CG   CD   OE1  OE2                                  
REMARK 470     ARG D 268    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ILE D 269    CG1  CG2  CD1                                       
REMARK 470     LYS D 270    CG   CD   CE   NZ                                   
REMARK 470     ARG D 277    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS D 327    CG   CD   CE   NZ                                   
REMARK 470     ARG A 161    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG A 162    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 163    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 164    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS A 166    CG   CD   CE   NZ                                   
REMARK 470     ARG A 175    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS A 206    CG   CD   CE   NZ                                   
REMARK 470     GLU A 209    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 219    CG   CD   CE   NZ                                   
REMARK 470     GLN B 266    CG   CD   OE1  NE2                                  
REMARK 470     GLU B 267    CG   CD   OE1  OE2                                  
REMARK 470     ARG B 268    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ILE B 269    CG1  CG2  CD1                                       
REMARK 470     LYS B 270    CG   CD   CE   NZ                                   
REMARK 470     GLU B 272    CG   CD   OE1  OE2                                  
REMARK 470     ARG B 273    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS B 274    CG   CD   CE   NZ                                   
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL D 401                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL D 404                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 302                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 303                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 305                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL B 401                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC7                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL B 402                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC8                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL B 403                  
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5VPA   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPC   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPD   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPE   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPF   RELATED DB: PDB                                   
DBREF  5VPB C  153   219  UNP    P53539   FOSB_HUMAN     153    219             
DBREF  5VPB D  266   332  UNP    P17535   JUND_HUMAN     266    332             
DBREF  5VPB A  153   219  UNP    P53539   FOSB_HUMAN     153    219             
DBREF  5VPB B  266   332  UNP    P17535   JUND_HUMAN     266    332             
SEQADV 5VPB SER C  152  UNP  P53539              EXPRESSION TAG                 
SEQADV 5VPB SER D  265  UNP  P17535              EXPRESSION TAG                 
SEQADV 5VPB SER A  152  UNP  P53539              EXPRESSION TAG                 
SEQADV 5VPB SER B  265  UNP  P17535              EXPRESSION TAG                 
SEQRES   1 C   68  SER GLU GLU GLU GLU LYS ARG ARG VAL ARG ARG GLU ARG          
SEQRES   2 C   68  ASN LYS LEU ALA ALA ALA LYS CYS ARG ASN ARG ARG ARG          
SEQRES   3 C   68  GLU LEU THR ASP ARG LEU GLN ALA GLU THR ASP GLN LEU          
SEQRES   4 C   68  GLU GLU GLU LYS ALA GLU LEU GLU SER GLU ILE ALA GLU          
SEQRES   5 C   68  LEU GLN LYS GLU LYS GLU ARG LEU GLU PHE VAL LEU VAL          
SEQRES   6 C   68  ALA HIS LYS                                                  
SEQRES   1 D   68  SER GLN GLU ARG ILE LYS ALA GLU ARG LYS ARG LEU ARG          
SEQRES   2 D   68  ASN ARG ILE ALA ALA SER LYS CYS ARG LYS ARG LYS LEU          
SEQRES   3 D   68  GLU ARG ILE SER ARG LEU GLU GLU LYS VAL LYS THR LEU          
SEQRES   4 D   68  LYS SER GLN ASN THR GLU LEU ALA SER THR ALA SER LEU          
SEQRES   5 D   68  LEU ARG GLU GLN VAL ALA GLN LEU LYS GLN LYS VAL LEU          
SEQRES   6 D   68  SER HIS VAL                                                  
SEQRES   1 A   68  SER GLU GLU GLU GLU LYS ARG ARG VAL ARG ARG GLU ARG          
SEQRES   2 A   68  ASN LYS LEU ALA ALA ALA LYS CYS ARG ASN ARG ARG ARG          
SEQRES   3 A   68  GLU LEU THR ASP ARG LEU GLN ALA GLU THR ASP GLN LEU          
SEQRES   4 A   68  GLU GLU GLU LYS ALA GLU LEU GLU SER GLU ILE ALA GLU          
SEQRES   5 A   68  LEU GLN LYS GLU LYS GLU ARG LEU GLU PHE VAL LEU VAL          
SEQRES   6 A   68  ALA HIS LYS                                                  
SEQRES   1 B   68  SER GLN GLU ARG ILE LYS ALA GLU ARG LYS ARG LEU ARG          
SEQRES   2 B   68  ASN ARG ILE ALA ALA SER LYS CYS ARG LYS ARG LYS LEU          
SEQRES   3 B   68  GLU ARG ILE SER ARG LEU GLU GLU LYS VAL LYS THR LEU          
SEQRES   4 B   68  LYS SER GLN ASN THR GLU LEU ALA SER THR ALA SER LEU          
SEQRES   5 B   68  LEU ARG GLU GLN VAL ALA GLN LEU LYS GLN LYS VAL LEU          
SEQRES   6 B   68  SER HIS VAL                                                  
HET     CL  D 401       1                                                       
HET     CL  D 402       1                                                       
HET     CL  D 403       1                                                       
HET     CL  D 404       1                                                       
HET     CL  A 301       1                                                       
HET     CL  A 302       1                                                       
HET     CL  A 303       1                                                       
HET     CL  A 304       1                                                       
HET     CL  A 305       1                                                       
HET     CL  B 401       1                                                       
HET     CL  B 402       1                                                       
HET     CL  B 403       1                                                       
HET     CL  B 404       1                                                       
HETNAM      CL CHLORIDE ION                                                     
FORMUL   5   CL    13(CL 1-)                                                    
FORMUL  18  HOH   *11(H2 O)                                                     
HELIX    1 AA1 GLU C  163  ARG C  175  1                                  13    
HELIX    2 AA2 ARG C  176  VAL C  216  1                                  41    
HELIX    3 AA3 ARG D  268  HIS D  331  1                                  64    
HELIX    4 AA4 ARG A  162  ARG A  175  1                                  14    
HELIX    5 AA5 ARG A  176  HIS A  218  1                                  43    
HELIX    6 AA6 GLU B  267  HIS B  331  1                                  65    
SSBOND   1 CYS C  172    CYS D  285                          1555   1555  2.03  
SSBOND   2 CYS A  172    CYS B  285                          1555   1555  2.03  
SITE     1 AC1  1 ARG D 288                                                     
SITE     1 AC2  1 LYS D 289                                                     
SITE     1 AC3  1 ARG A 210                                                     
SITE     1 AC4  1 ARG B 318                                                     
SITE     1 AC5  3 GLN A 184  ARG C 173  ARG D 292                               
SITE     1 AC6  1 LYS B 289                                                     
SITE     1 AC7  1 SER B 283                                                     
SITE     1 AC8  1 GLN B 323                                                     
CRYST1   92.825   99.261   49.860  90.00 109.40  90.00 C 1 2 1       8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.010773  0.000000  0.003794        0.00000                         
SCALE2      0.000000  0.010074  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.021264        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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