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Database: PDB
Entry: 5VPE
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HEADER    TRANSCRIPTION/DNA                       04-MAY-17   5VPE              
TITLE     TRANSCRIPTION FACTOR FOSB/JUND BZIP DOMAIN IN COMPLEX WITH COGNATE    
TITLE    2 DNA, TYPE-I CRYSTAL                                                  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN FOSB;                                              
COMPND   3 CHAIN: C, A;                                                         
COMPND   4 FRAGMENT: UNP RESIDUES 153-219;                                      
COMPND   5 SYNONYM: G0/G1 SWITCH REGULATORY PROTEIN 3;                          
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: TRANSCRIPTION FACTOR JUN-D;                                
COMPND   9 CHAIN: D, B;                                                         
COMPND  10 FRAGMENT: UNP RESIDUES 266-332;                                      
COMPND  11 ENGINEERED: YES;                                                     
COMPND  12 MOL_ID: 3;                                                           
COMPND  13 MOLECULE: DNA (5'-                                                   
COMPND  14 D(*CP*GP*TP*CP*GP*GP*TP*GP*AP*CP*TP*CP*AP*CP*CP*GP*AP*CP*G)-3');     
COMPND  15 CHAIN: G, E;                                                         
COMPND  16 ENGINEERED: YES;                                                     
COMPND  17 MOL_ID: 4;                                                           
COMPND  18 MOLECULE: DNA (5'-                                                   
COMPND  19 D(*CP*GP*TP*CP*GP*GP*TP*GP*AP*GP*TP*CP*AP*CP*CP*GP*AP*CP*G)-3');     
COMPND  20 CHAIN: H, F;                                                         
COMPND  21 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: FOSB, G0S3;                                                    
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: ROSETTA 2 (DE3);                           
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET21-NESG;                               
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  13 ORGANISM_COMMON: HUMAN;                                              
SOURCE  14 ORGANISM_TAXID: 9606;                                                
SOURCE  15 GENE: JUND;                                                          
SOURCE  16 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  17 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  18 EXPRESSION_SYSTEM_STRAIN: ROSETTA 2 (DE3);                           
SOURCE  19 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  20 EXPRESSION_SYSTEM_PLASMID: PET21A;                                   
SOURCE  21 MOL_ID: 3;                                                           
SOURCE  22 SYNTHETIC: YES;                                                      
SOURCE  23 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  24 ORGANISM_TAXID: 9606;                                                
SOURCE  25 MOL_ID: 4;                                                           
SOURCE  26 SYNTHETIC: YES;                                                      
SOURCE  27 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  28 ORGANISM_TAXID: 9606                                                 
KEYWDS    ACTIVATOR PROTEIN-1, BASIC LEUCINE ZIPPER, BZIP, FOS, JUN,            
KEYWDS   2 TRANSCRIPTION FACTOR, DNA-BINDING PROTEIN, REDOX SWITCH, COILED-     
KEYWDS   3 COIL, TRANSCRIPTION-DNA COMPLEX                                      
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Z.YIN,M.MACHIUS,G.RUDENKO                                             
REVDAT   4   11-DEC-19 5VPE    1       REMARK                                   
REVDAT   3   15-NOV-17 5VPE    1       JRNL                                     
REVDAT   2   18-OCT-17 5VPE    1       JRNL                                     
REVDAT   1   06-SEP-17 5VPE    0                                                
JRNL        AUTH   Z.YIN,M.MACHIUS,E.J.NESTLER,G.RUDENKO                        
JRNL        TITL   ACTIVATOR PROTEIN-1: REDOX SWITCH CONTROLLING STRUCTURE AND  
JRNL        TITL 2 DNA-BINDING.                                                 
JRNL        REF    NUCLEIC ACIDS RES.            V.  45 11425 2017              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   28981703                                                     
JRNL        DOI    10.1093/NAR/GKX795                                           
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.05 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.11.1_2575                                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.05                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 47.31                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.360                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 87.5                           
REMARK   3   NUMBER OF REFLECTIONS             : 53064                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.219                           
REMARK   3   R VALUE            (WORKING SET) : 0.218                           
REMARK   3   FREE R VALUE                     : 0.254                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 3.770                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 2000                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 47.3194 -  4.9473    0.97     4138   162  0.2145 0.2524        
REMARK   3     2  4.9473 -  3.9274    0.99     4179   164  0.1732 0.2207        
REMARK   3     3  3.9274 -  3.4312    0.97     4091   160  0.1843 0.2052        
REMARK   3     4  3.4312 -  3.1175    0.98     4072   160  0.2087 0.2668        
REMARK   3     5  3.1175 -  2.8941    0.99     4142   163  0.2355 0.2611        
REMARK   3     6  2.8941 -  2.7235    0.99     4118   160  0.2436 0.2716        
REMARK   3     7  2.7235 -  2.5871    0.97     4050   159  0.2378 0.2674        
REMARK   3     8  2.5871 -  2.4745    0.99     4093   161  0.2365 0.2650        
REMARK   3     9  2.4745 -  2.3792    0.97     4046   158  0.2425 0.2978        
REMARK   3    10  2.3792 -  2.2971    0.90     3743   146  0.2458 0.3043        
REMARK   3    11  2.2971 -  2.2253    0.79     3306   130  0.2475 0.2595        
REMARK   3    12  2.2253 -  2.1617    0.69     2834   110  0.2655 0.2669        
REMARK   3    13  2.1617 -  2.1048    0.62     2557   101  0.2864 0.2927        
REMARK   3    14  2.1048 -  2.0534    0.41     1695    66  0.2998 0.3857        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.260            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 28.540           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 24.63                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 41.45                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.005           3990                                  
REMARK   3   ANGLE     :  0.747           5638                                  
REMARK   3   CHIRALITY :  0.036            631                                  
REMARK   3   PLANARITY :  0.003            479                                  
REMARK   3   DIHEDRAL  : 20.771           2289                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 15                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 153 THROUGH 218 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -32.0801  -7.5777 108.0989              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3168 T22:   0.2768                                     
REMARK   3      T33:   0.2450 T12:  -0.2253                                     
REMARK   3      T13:   0.0921 T23:  -0.1844                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.2029 L22:   0.3370                                     
REMARK   3      L33:   5.2113 L12:  -0.1862                                     
REMARK   3      L13:  -0.4450 L23:   0.5059                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0110 S12:  -0.0402 S13:   0.1482                       
REMARK   3      S21:   0.0443 S22:  -0.2117 S23:  -0.0562                       
REMARK   3      S31:  -0.2305 S32:   0.2177 S33:   0.0079                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 265 THROUGH 331 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -35.7705 -15.8024 107.2282              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2881 T22:   0.1672                                     
REMARK   3      T33:   0.1999 T12:  -0.1291                                     
REMARK   3      T13:   0.1087 T23:  -0.0666                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.4873 L22:   0.5752                                     
REMARK   3      L33:   5.8853 L12:   0.7289                                     
REMARK   3      L13:  -3.3542 L23:  -1.3677                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0330 S12:   0.1238 S13:   0.1009                       
REMARK   3      S21:  -0.0383 S22:   0.0488 S23:  -0.0995                       
REMARK   3      S31:   0.0184 S32:   0.2940 S33:  -0.0025                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'G' AND (RESID 2 THROUGH 6 )                     
REMARK   3    ORIGIN FOR THE GROUP (A): -26.1541 -10.2071 138.9993              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3576 T22:   0.8998                                     
REMARK   3      T33:   0.8181 T12:  -0.1276                                     
REMARK   3      T13:  -0.2339 T23:  -0.1340                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.7642 L22:   2.3400                                     
REMARK   3      L33:   2.8762 L12:  -1.5182                                     
REMARK   3      L13:   1.8795 L23:   0.1484                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2058 S12:  -0.9571 S13:   0.1387                       
REMARK   3      S21:   0.7872 S22:  -0.0938 S23:  -1.0750                       
REMARK   3      S31:  -0.5002 S32:   0.6880 S33:   0.3253                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'G' AND (RESID 7 THROUGH 16 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -49.1441 -12.4087 129.4877              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2623 T22:   0.1197                                     
REMARK   3      T33:   0.3091 T12:  -0.0190                                     
REMARK   3      T13:   0.0010 T23:  -0.0791                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.2842 L22:   2.5184                                     
REMARK   3      L33:   2.5993 L12:   1.6791                                     
REMARK   3      L13:   0.2496 L23:  -0.2040                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0238 S12:  -0.0605 S13:   0.3703                       
REMARK   3      S21:  -0.0905 S22:   0.2280 S23:   0.0097                       
REMARK   3      S31:  -0.2763 S32:   0.0245 S33:  -0.1399                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'G' AND (RESID 17 THROUGH 19 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -66.2743 -14.1117 116.5837              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2983 T22:   0.3840                                     
REMARK   3      T33:   0.3817 T12:  -0.1412                                     
REMARK   3      T13:   0.0367 T23:   0.0164                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.7715 L22:   2.1733                                     
REMARK   3      L33:   6.3532 L12:   2.1720                                     
REMARK   3      L13:  -3.9366 L23:  -3.2686                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.9227 S12:   1.1372 S13:  -0.3351                       
REMARK   3      S21:  -1.0565 S22:   0.2965 S23:  -0.8724                       
REMARK   3      S31:  -0.2846 S32:   0.1859 S33:   0.5747                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'H' AND (RESID 1 THROUGH 15 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -50.5709 -13.3640 129.7484              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2470 T22:   0.0968                                     
REMARK   3      T33:   0.2968 T12:  -0.0482                                     
REMARK   3      T13:  -0.0078 T23:  -0.0478                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.2193 L22:   1.5413                                     
REMARK   3      L33:   2.8597 L12:   1.2614                                     
REMARK   3      L13:   2.1459 L23:   0.4091                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0303 S12:  -0.2597 S13:  -0.1661                       
REMARK   3      S21:   0.0148 S22:   0.1912 S23:  -0.2056                       
REMARK   3      S31:   0.0707 S32:   0.0366 S33:  -0.2056                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: CHAIN 'H' AND (RESID 16 THROUGH 18 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -21.3751 -14.5701 133.7590              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3391 T22:   1.1868                                     
REMARK   3      T33:   1.1278 T12:  -0.0778                                     
REMARK   3      T13:  -0.2458 T23:  -0.0104                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.0000 L22:   1.2678                                     
REMARK   3      L33:   5.3534 L12:  -2.8768                                     
REMARK   3      L13:  -0.6501 L23:  -1.3568                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0292 S12:   0.4349 S13:   0.0486                       
REMARK   3      S21:  -0.0277 S22:   0.1895 S23:  -0.6126                       
REMARK   3      S31:  -0.1538 S32:   0.7049 S33:  -0.1845                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 152 THROUGH 218 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -71.8272 -38.3745  88.1260              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3288 T22:   0.2792                                     
REMARK   3      T33:   0.2621 T12:  -0.1523                                     
REMARK   3      T13:   0.0946 T23:  -0.0347                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.1817 L22:   0.5811                                     
REMARK   3      L33:   4.2043 L12:   1.3138                                     
REMARK   3      L13:  -4.2020 L23:  -1.4210                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0516 S12:  -0.2430 S13:  -0.4667                       
REMARK   3      S21:  -0.0381 S22:  -0.1982 S23:   0.0607                       
REMARK   3      S31:   0.1968 S32:   0.1098 S33:   0.3426                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 265 THROUGH 331 )                 
REMARK   3    ORIGIN FOR THE GROUP (A): -68.7005 -29.5295  90.0838              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3397 T22:   0.3337                                     
REMARK   3      T33:   0.2021 T12:  -0.1555                                     
REMARK   3      T13:   0.1029 T23:  -0.0065                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.2824 L22:   0.3193                                     
REMARK   3      L33:   6.5641 L12:  -0.4764                                     
REMARK   3      L13:  -2.6356 L23:   1.1724                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1617 S12:   0.0726 S13:  -0.0563                       
REMARK   3      S21:  -0.0683 S22:   0.0972 S23:  -0.0285                       
REMARK   3      S31:   0.0294 S32:  -0.2619 S33:  -0.1993                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 2 THROUGH 6 )                     
REMARK   3    ORIGIN FOR THE GROUP (A):-100.2146 -37.4599 100.5874              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4383 T22:   0.8737                                     
REMARK   3      T33:   0.6444 T12:  -0.1064                                     
REMARK   3      T13:  -0.3588 T23:  -0.0948                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.5250 L22:   2.2130                                     
REMARK   3      L33:   1.1399 L12:  -0.3981                                     
REMARK   3      L13:   1.0272 L23:  -0.4372                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0510 S12:   0.3177 S13:  -0.3131                       
REMARK   3      S21:  -0.4097 S22:   0.0474 S23:   0.8644                       
REMARK   3      S31:   0.0699 S32:  -0.7569 S33:  -0.0835                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 7 THROUGH 16 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -79.4712 -33.3521 113.2224              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2472 T22:   0.2651                                     
REMARK   3      T33:   0.2417 T12:  -0.1077                                     
REMARK   3      T13:  -0.0039 T23:   0.0313                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.3978 L22:   3.1515                                     
REMARK   3      L33:   1.6106 L12:   0.9757                                     
REMARK   3      L13:   1.6311 L23:   1.4474                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1302 S12:  -0.0338 S13:  -0.3902                       
REMARK   3      S21:  -0.0357 S22:   0.2612 S23:   0.0612                       
REMARK   3      S31:   0.2690 S32:  -0.0209 S33:  -0.3083                       
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    SELECTION: CHAIN 'E' AND (RESID 17 THROUGH 19 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -59.1488 -30.7201 119.9631              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4228 T22:   0.2832                                     
REMARK   3      T33:   0.2407 T12:  -0.0232                                     
REMARK   3      T13:  -0.0894 T23:  -0.0643                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.3992 L22:   2.3946                                     
REMARK   3      L33:   1.8411 L12:   1.1275                                     
REMARK   3      L13:   0.9578 L23:   2.0991                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1565 S12:   0.8209 S13:  -0.6007                       
REMARK   3      S21:  -0.9810 S22:   0.2438 S23:  -0.0445                       
REMARK   3      S31:  -0.5769 S32:   0.2847 S33:  -0.0729                       
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 1 THROUGH 5 )                     
REMARK   3    ORIGIN FOR THE GROUP (A): -66.3794 -30.3006 124.5962              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2125 T22:   0.4041                                     
REMARK   3      T33:   0.2937 T12:  -0.1114                                     
REMARK   3      T13:  -0.0292 T23:  -0.0333                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.4002 L22:   3.7193                                     
REMARK   3      L33:   9.5321 L12:  -1.4803                                     
REMARK   3      L13:   5.9580 L23:   0.1675                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3759 S12:  -0.2658 S13:  -0.0926                       
REMARK   3      S21:   0.2721 S22:  -0.1172 S23:   0.3243                       
REMARK   3      S31:  -0.4751 S32:  -0.6679 S33:   0.4855                       
REMARK   3   TLS GROUP : 14                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 6 THROUGH 15 )                    
REMARK   3    ORIGIN FOR THE GROUP (A): -84.8997 -33.5654 109.8931              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2731 T22:   0.3396                                     
REMARK   3      T33:   0.2453 T12:  -0.1765                                     
REMARK   3      T13:  -0.0367 T23:   0.0578                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.2200 L22:   3.1724                                     
REMARK   3      L33:   1.9069 L12:   1.5244                                     
REMARK   3      L13:  -0.0393 L23:   2.0402                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1251 S12:   0.3766 S13:   0.1440                       
REMARK   3      S21:  -0.2126 S22:   0.0548 S23:   0.2388                       
REMARK   3      S31:  -0.1432 S32:  -0.2102 S33:   0.2180                       
REMARK   3   TLS GROUP : 15                                                     
REMARK   3    SELECTION: CHAIN 'F' AND (RESID 16 THROUGH 18 )                   
REMARK   3    ORIGIN FOR THE GROUP (A): -99.4154 -32.9763  93.5124              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5732 T22:   1.0970                                     
REMARK   3      T33:   0.7281 T12:  -0.0650                                     
REMARK   3      T13:  -0.4056 T23:  -0.0247                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.6652 L22:   0.5542                                     
REMARK   3      L33:   3.9154 L12:   0.5201                                     
REMARK   3      L13:   3.0558 L23:   0.8543                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1862 S12:   0.4854 S13:   0.1234                       
REMARK   3      S21:  -0.3507 S22:   0.2517 S23:  -0.0978                       
REMARK   3      S31:  -0.0943 S32:   0.4112 S33:  -0.0404                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5VPE COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 05-MAY-17.                  
REMARK 100 THE DEPOSITION ID IS D_1000227770.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 16-JUL-16                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 19-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.979                              
REMARK 200  MONOCHROMATOR                  : SI(111)                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M                 
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 58932                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.060                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.3                               
REMARK 200  DATA REDUNDANCY                : 6.100                              
REMARK 200  R MERGE                    (I) : 0.05500                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 15.9000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.06                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.10                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 98.2                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 5.90                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.93100                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 1FOS                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 72.16                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 4.42                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.9 M NA2HPO4 AND 24% (V/V) ETHYLENE     
REMARK 280  GLYCOL, PH 5.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       58.23950            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       45.37750            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       58.23950            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       45.37750            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 8550 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 14770 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -83.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D, G, H                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 8230 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 15120 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -59.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, E, F                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER C   152                                                      
REMARK 465     LYS C   219                                                      
REMARK 465     VAL D   332                                                      
REMARK 465      DC G     1                                                      
REMARK 465      DG H    19                                                      
REMARK 465     LYS A   219                                                      
REMARK 465     VAL B   332                                                      
REMARK 465      DC E     1                                                      
REMARK 465      DG F    19                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLU C 153    CG   CD   OE1  OE2                                  
REMARK 470     GLU C 154    CG   CD   OE1  OE2                                  
REMARK 470     LYS C 157    CG   CD   CE   NZ                                   
REMARK 470     ARG C 158    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU C 203    CG   CD   OE1  OE2                                  
REMARK 470     LYS C 206    CG   CD   CE   NZ                                   
REMARK 470     HIS C 218    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLU D 267    CG   CD   OE1  OE2                                  
REMARK 470     LEU D 329    CG   CD1  CD2                                       
REMARK 470     SER A 152    OG                                                  
REMARK 470     HIS A 218    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LYS B 287    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   H22   DG E     2     O2    DC F    18              1.58            
REMARK 500   OE2  GLU A   156    HH21  ARG A   159              1.59            
REMARK 500   OE1  GLU B   298     O2   EDO B   404              2.12            
REMARK 500   NH1  ARG B   288     O    HOH B   501              2.16            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500     DC G  18   O4' -  C1' -  N1  ANGL. DEV. =   2.2 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 C 301                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 302                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 D 401                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO D 402                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO D 403                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL D 404                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC7                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO G 101                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC8                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 301                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC9                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 302                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 303                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 401                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 402                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 403                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 404                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 F 101                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5VPA   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPB   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPC   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPD   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPF   RELATED DB: PDB                                   
DBREF  5VPE C  153   219  UNP    P53539   FOSB_HUMAN     153    219             
DBREF  5VPE D  266   332  UNP    P17535   JUND_HUMAN     266    332             
DBREF  5VPE G    1    19  PDB    5VPE     5VPE             1     19             
DBREF  5VPE H    1    19  PDB    5VPE     5VPE             1     19             
DBREF  5VPE A  153   219  UNP    P53539   FOSB_HUMAN     153    219             
DBREF  5VPE B  266   332  UNP    P17535   JUND_HUMAN     266    332             
DBREF  5VPE E    1    19  PDB    5VPE     5VPE             1     19             
DBREF  5VPE F    1    19  PDB    5VPE     5VPE             1     19             
SEQADV 5VPE SER C  152  UNP  P53539              EXPRESSION TAG                 
SEQADV 5VPE SER D  265  UNP  P17535              EXPRESSION TAG                 
SEQADV 5VPE SER A  152  UNP  P53539              EXPRESSION TAG                 
SEQADV 5VPE SER B  265  UNP  P17535              EXPRESSION TAG                 
SEQRES   1 C   68  SER GLU GLU GLU GLU LYS ARG ARG VAL ARG ARG GLU ARG          
SEQRES   2 C   68  ASN LYS LEU ALA ALA ALA LYS CYS ARG ASN ARG ARG ARG          
SEQRES   3 C   68  GLU LEU THR ASP ARG LEU GLN ALA GLU THR ASP GLN LEU          
SEQRES   4 C   68  GLU GLU GLU LYS ALA GLU LEU GLU SER GLU ILE ALA GLU          
SEQRES   5 C   68  LEU GLN LYS GLU LYS GLU ARG LEU GLU PHE VAL LEU VAL          
SEQRES   6 C   68  ALA HIS LYS                                                  
SEQRES   1 D   68  SER GLN GLU ARG ILE LYS ALA GLU ARG LYS ARG LEU ARG          
SEQRES   2 D   68  ASN ARG ILE ALA ALA SER LYS CYS ARG LYS ARG LYS LEU          
SEQRES   3 D   68  GLU ARG ILE SER ARG LEU GLU GLU LYS VAL LYS THR LEU          
SEQRES   4 D   68  LYS SER GLN ASN THR GLU LEU ALA SER THR ALA SER LEU          
SEQRES   5 D   68  LEU ARG GLU GLN VAL ALA GLN LEU LYS GLN LYS VAL LEU          
SEQRES   6 D   68  SER HIS VAL                                                  
SEQRES   1 G   19   DC  DG  DT  DC  DG  DG  DT  DG  DA  DC  DT  DC  DA          
SEQRES   2 G   19   DC  DC  DG  DA  DC  DG                                      
SEQRES   1 H   19   DC  DG  DT  DC  DG  DG  DT  DG  DA  DG  DT  DC  DA          
SEQRES   2 H   19   DC  DC  DG  DA  DC  DG                                      
SEQRES   1 A   68  SER GLU GLU GLU GLU LYS ARG ARG VAL ARG ARG GLU ARG          
SEQRES   2 A   68  ASN LYS LEU ALA ALA ALA LYS CYS ARG ASN ARG ARG ARG          
SEQRES   3 A   68  GLU LEU THR ASP ARG LEU GLN ALA GLU THR ASP GLN LEU          
SEQRES   4 A   68  GLU GLU GLU LYS ALA GLU LEU GLU SER GLU ILE ALA GLU          
SEQRES   5 A   68  LEU GLN LYS GLU LYS GLU ARG LEU GLU PHE VAL LEU VAL          
SEQRES   6 A   68  ALA HIS LYS                                                  
SEQRES   1 B   68  SER GLN GLU ARG ILE LYS ALA GLU ARG LYS ARG LEU ARG          
SEQRES   2 B   68  ASN ARG ILE ALA ALA SER LYS CYS ARG LYS ARG LYS LEU          
SEQRES   3 B   68  GLU ARG ILE SER ARG LEU GLU GLU LYS VAL LYS THR LEU          
SEQRES   4 B   68  LYS SER GLN ASN THR GLU LEU ALA SER THR ALA SER LEU          
SEQRES   5 B   68  LEU ARG GLU GLN VAL ALA GLN LEU LYS GLN LYS VAL LEU          
SEQRES   6 B   68  SER HIS VAL                                                  
SEQRES   1 E   19   DC  DG  DT  DC  DG  DG  DT  DG  DA  DC  DT  DC  DA          
SEQRES   2 E   19   DC  DC  DG  DA  DC  DG                                      
SEQRES   1 F   19   DC  DG  DT  DC  DG  DG  DT  DG  DA  DG  DT  DC  DA          
SEQRES   2 F   19   DC  DC  DG  DA  DC  DG                                      
HET    PO4  C 301       5                                                       
HET    EDO  C 302      10                                                       
HET     NA  C 303       1                                                       
HET    PO4  D 401       5                                                       
HET    EDO  D 402      10                                                       
HET    EDO  D 403      10                                                       
HET     CL  D 404       1                                                       
HET    EDO  G 101      10                                                       
HET     NA  G 102       1                                                       
HET    EDO  A 301      10                                                       
HET    EDO  A 302      10                                                       
HET    EDO  A 303      10                                                       
HET    EDO  B 401      10                                                       
HET    EDO  B 402      10                                                       
HET    EDO  B 403      10                                                       
HET    EDO  B 404      10                                                       
HET     NA  E 101       1                                                       
HET    PO4  F 101       5                                                       
HETNAM     PO4 PHOSPHATE ION                                                    
HETNAM     EDO 1,2-ETHANEDIOL                                                   
HETNAM      NA SODIUM ION                                                       
HETNAM      CL CHLORIDE ION                                                     
HETSYN     EDO ETHYLENE GLYCOL                                                  
FORMUL   9  PO4    3(O4 P 3-)                                                   
FORMUL  10  EDO    11(C2 H6 O2)                                                 
FORMUL  11   NA    3(NA 1+)                                                     
FORMUL  15   CL    CL 1-                                                        
FORMUL  27  HOH   *228(H2 O)                                                    
HELIX    1 AA1 GLU C  156  HIS C  218  1                                  63    
HELIX    2 AA2 GLN D  266  SER D  330  1                                  65    
HELIX    3 AA3 GLU A  153  HIS A  218  1                                  66    
HELIX    4 AA4 GLN B  266  HIS B  331  1                                  66    
SITE     1 AC1  2 GLU C 155  ARG C 162                                          
SITE     1 AC2  2 GLU C 191  LYS C 194                                          
SITE     1 AC3  8 CYS D 285  ARG D 286  LYS D 289  HOH D 501                    
SITE     2 AC3  8 HOH D 504   DT H   7   DG H   8   DA H   9                    
SITE     1 AC4  3 GLN D 266  ARG D 273   DG F   2                               
SITE     1 AC5  2 ARG D 268  ARG D 275                                          
SITE     1 AC6  1 ARG D 279                                                     
SITE     1 AC7  4  DG G   8   DA G   9   DA H  13   DC H  14                    
SITE     1 AC8  4 GLU A 156  LYS A 157  HOH A 419  ASP C 181                    
SITE     1 AC9  2 LYS A 194  GLU A 198                                          
SITE     1 AD1  2 GLU A 191  LYS A 194                                          
SITE     1 AD2  1 GLU B 267                                                     
SITE     1 AD3  2 SER B 283   DC E  10                                          
SITE     1 AD4  1 SER B 265                                                     
SITE     1 AD5  4 GLU B 297  GLU B 298  LYS B 301  HOH B 518                    
SITE     1 AD6  6 ARG B 286  LYS B 289   DT F   7   DG F   8                    
SITE     2 AD6  6  DA F   9  HOH F 206                                          
CRYST1  116.479   90.755  105.618  90.00 117.93  90.00 C 1 2 1       8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.008585  0.000000  0.004552        0.00000                         
SCALE2      0.000000  0.011019  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.010717        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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