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Entry: 5VPF
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HEADER    TRANSCRIPTION/DNA                       04-MAY-17   5VPF              
TITLE     TRANSCRIPTION FACTOR FOSB/JUND BZIP DOMAIN IN COMPLEX WITH COGNATE    
TITLE    2 DNA, TYPE-II CRYSTAL                                                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN FOSB;                                              
COMPND   3 CHAIN: A, C;                                                         
COMPND   4 FRAGMENT: UNP RESIDUES 153-219;                                      
COMPND   5 SYNONYM: G0/G1 SWITCH REGULATORY PROTEIN 3;                          
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: TRANSCRIPTION FACTOR JUN-D;                                
COMPND   9 CHAIN: B, D;                                                         
COMPND  10 FRAGMENT: UNP RESIDUES 266-332;                                      
COMPND  11 ENGINEERED: YES;                                                     
COMPND  12 MOL_ID: 3;                                                           
COMPND  13 MOLECULE: DNA (5'-                                                   
COMPND  14 D(*CP*GP*TP*CP*GP*GP*TP*GP*AP*CP*TP*CP*AP*CP*CP*GP*AP*CP*G)-3');     
COMPND  15 CHAIN: E, G;                                                         
COMPND  16 ENGINEERED: YES;                                                     
COMPND  17 MOL_ID: 4;                                                           
COMPND  18 MOLECULE: DNA (5'-                                                   
COMPND  19 D(*CP*GP*TP*CP*GP*GP*TP*GP*AP*GP*TP*CP*AP*CP*CP*GP*AP*CP*G)-3');     
COMPND  20 CHAIN: F, H;                                                         
COMPND  21 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: FOSB, G0S3;                                                    
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: ROSETTA 2 (DE3);                           
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET21-NESG;                               
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  13 ORGANISM_COMMON: HUMAN;                                              
SOURCE  14 ORGANISM_TAXID: 9606;                                                
SOURCE  15 GENE: JUND;                                                          
SOURCE  16 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  17 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  18 EXPRESSION_SYSTEM_STRAIN: ROSETTA 2 (DE3);                           
SOURCE  19 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  20 EXPRESSION_SYSTEM_PLASMID: PET21A;                                   
SOURCE  21 MOL_ID: 3;                                                           
SOURCE  22 SYNTHETIC: YES;                                                      
SOURCE  23 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  24 ORGANISM_TAXID: 9606;                                                
SOURCE  25 MOL_ID: 4;                                                           
SOURCE  26 SYNTHETIC: YES;                                                      
SOURCE  27 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  28 ORGANISM_TAXID: 9606                                                 
KEYWDS    ACTIVATOR PROTEIN-1, BASIC LEUCINE ZIPPER, BZIP, FOS, JUN,            
KEYWDS   2 TRANSCRIPTION FACTOR, DNA-BINDING PROTEIN, REDOX SWITCH, COILED-     
KEYWDS   3 COIL, TRANSCRIPTION-DNA COMPLEX                                      
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Z.YIN,G.RUDENKO,M.MACHIUS                                             
REVDAT   4   11-DEC-19 5VPF    1       REMARK                                   
REVDAT   3   15-NOV-17 5VPF    1       JRNL                                     
REVDAT   2   18-OCT-17 5VPF    1       JRNL                                     
REVDAT   1   06-SEP-17 5VPF    0                                                
JRNL        AUTH   Z.YIN,M.MACHIUS,E.J.NESTLER,G.RUDENKO                        
JRNL        TITL   ACTIVATOR PROTEIN-1: REDOX SWITCH CONTROLLING STRUCTURE AND  
JRNL        TITL 2 DNA-BINDING.                                                 
JRNL        REF    NUCLEIC ACIDS RES.            V.  45 11425 2017              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   28981703                                                     
JRNL        DOI    10.1093/NAR/GKX795                                           
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.69 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.11.1_2575                                   
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.69                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 48.59                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.360                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 90.9                           
REMARK   3   NUMBER OF REFLECTIONS             : 27337                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.213                           
REMARK   3   R VALUE            (WORKING SET) : 0.210                           
REMARK   3   FREE R VALUE                     : 0.248                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 7.310                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1999                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 48.6008 -  6.4871    0.99     2194   173  0.2103 0.2114        
REMARK   3     2  6.4871 -  5.1508    1.00     2052   163  0.2158 0.2387        
REMARK   3     3  5.1508 -  4.5003    1.00     2025   159  0.1918 0.2393        
REMARK   3     4  4.5003 -  4.0890    1.00     2008   158  0.1771 0.2201        
REMARK   3     5  4.0890 -  3.7961    1.00     1989   158  0.1735 0.2377        
REMARK   3     6  3.7961 -  3.5723    1.00     1966   154  0.2092 0.2527        
REMARK   3     7  3.5723 -  3.3935    1.00     1966   156  0.1945 0.2658        
REMARK   3     8  3.3935 -  3.2458    0.97     1903   150  0.2063 0.2376        
REMARK   3     9  3.2458 -  3.1209    0.92     1803   143  0.2238 0.2616        
REMARK   3    10  3.1209 -  3.0132    0.87     1697   132  0.2576 0.2966        
REMARK   3    11  3.0132 -  2.9190    0.81     1580   125  0.2712 0.3413        
REMARK   3    12  2.9190 -  2.8356    0.77     1497   118  0.2792 0.3280        
REMARK   3    13  2.8356 -  2.7609    0.72     1397   111  0.2847 0.2698        
REMARK   3    14  2.7609 -  2.6936    0.65     1261    99  0.2763 0.3264        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.320            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 26.280           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 40.66                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 50.96                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.006           3938                                  
REMARK   3   ANGLE     :  0.792           5591                                  
REMARK   3   CHIRALITY :  0.040            635                                  
REMARK   3   PLANARITY :  0.004            460                                  
REMARK   3   DIHEDRAL  : 21.758           2227                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 14                                         
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: ( CHAIN A AND RESID 152:218 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):  -12.889   68.263  -35.607              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1565 T22:   0.2770                                     
REMARK   3      T33:   0.2006 T12:  -0.0812                                     
REMARK   3      T13:  -0.1167 T23:  -0.0370                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.6353 L22:   0.6334                                     
REMARK   3      L33:   0.4624 L12:   0.5130                                     
REMARK   3      L13:   0.5422 L23:   0.4900                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1408 S12:   0.3101 S13:  -0.0047                       
REMARK   3      S21:  -0.1568 S22:   0.2003 S23:  -0.0591                       
REMARK   3      S31:   0.0462 S32:  -0.0482 S33:   0.0456                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: ( CHAIN B AND RESID 266:332 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):   -8.815   69.169  -27.691              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2372 T22:   0.2257                                     
REMARK   3      T33:   0.1298 T12:   0.0022                                     
REMARK   3      T13:  -0.1152 T23:  -0.0127                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.5562 L22:   6.5279                                     
REMARK   3      L33:   1.0365 L12:   3.8348                                     
REMARK   3      L13:  -1.1340 L23:  -2.1819                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0334 S12:   0.1982 S13:   0.1132                       
REMARK   3      S21:   0.1652 S22:   0.0566 S23:   0.1045                       
REMARK   3      S31:  -0.0503 S32:   0.0182 S33:  -0.0852                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: ( CHAIN E AND RESID 1:5 )                              
REMARK   3    ORIGIN FOR THE GROUP (A):  -46.087   55.685  -32.149              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3331 T22:   0.7880                                     
REMARK   3      T33:   0.9369 T12:   0.2221                                     
REMARK   3      T13:  -0.1260 T23:  -0.0720                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.3017 L22:   2.8598                                     
REMARK   3      L33:   5.0086 L12:   2.8092                                     
REMARK   3      L13:  -3.7599 L23:   0.7649                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0225 S12:   0.1539 S13:  -0.3982                       
REMARK   3      S21:   0.1411 S22:   0.2596 S23:   1.1122                       
REMARK   3      S31:  -0.1900 S32:  -1.3655 S33:  -0.2952                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: ( CHAIN E AND RESID 6:19 )                             
REMARK   3    ORIGIN FOR THE GROUP (A):  -17.955   43.296  -28.585              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2393 T22:   0.3547                                     
REMARK   3      T33:   0.1853 T12:   0.0334                                     
REMARK   3      T13:  -0.0836 T23:  -0.1053                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.7566 L22:   0.6659                                     
REMARK   3      L33:   0.2245 L12:   0.8767                                     
REMARK   3      L13:  -0.0937 L23:  -0.2686                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1323 S12:   0.3424 S13:  -0.3498                       
REMARK   3      S21:  -0.0316 S22:  -0.0568 S23:  -0.1019                       
REMARK   3      S31:   0.0156 S32:   0.0505 S33:  -0.2712                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: ( CHAIN F AND RESID 1:10 )                             
REMARK   3    ORIGIN FOR THE GROUP (A):  -13.636   39.393  -28.835              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1854 T22:   0.3435                                     
REMARK   3      T33:   0.1929 T12:   0.0241                                     
REMARK   3      T13:   0.0152 T23:  -0.0313                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.1899 L22:   1.6529                                     
REMARK   3      L33:   3.1734 L12:  -0.2206                                     
REMARK   3      L13:   1.9805 L23:   0.2941                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1159 S12:   0.4175 S13:  -0.4901                       
REMARK   3      S21:   0.0742 S22:   0.0325 S23:   0.0087                       
REMARK   3      S31:   0.1576 S32:   0.1899 S33:   0.1023                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: ( CHAIN F AND RESID 11:15 )                            
REMARK   3    ORIGIN FOR THE GROUP (A):  -35.846   48.307  -25.500              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4440 T22:   0.4351                                     
REMARK   3      T33:   0.3412 T12:   0.1320                                     
REMARK   3      T13:   0.1271 T23:   0.0830                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   6.1985 L22:   5.5487                                     
REMARK   3      L33:   2.7554 L12:  -1.5196                                     
REMARK   3      L13:  -2.8558 L23:   3.3865                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1352 S12:  -0.7260 S13:   0.1354                       
REMARK   3      S21:   0.8049 S22:   0.0015 S23:   0.9647                       
REMARK   3      S31:  -0.0473 S32:  -0.7137 S33:   0.1266                       
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    SELECTION: ( CHAIN F AND RESID 16:19 )                            
REMARK   3    ORIGIN FOR THE GROUP (A):  -42.392   63.105  -29.099              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5620 T22:   0.6320                                     
REMARK   3      T33:   0.6373 T12:   0.3695                                     
REMARK   3      T13:  -0.0408 T23:  -0.0298                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.3930 L22:   0.6077                                     
REMARK   3      L33:   0.0380 L12:  -0.3904                                     
REMARK   3      L13:   0.1038 L23:  -0.1513                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3091 S12:  -0.4683 S13:   0.3648                       
REMARK   3      S21:   0.4948 S22:   0.2658 S23:  -0.2839                       
REMARK   3      S31:  -0.1134 S32:  -0.0352 S33:   0.1305                       
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    SELECTION: ( CHAIN C AND RESID 154:217 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):   27.520   50.679   -6.103              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3587 T22:   0.3203                                     
REMARK   3      T33:   0.4668 T12:   0.1217                                     
REMARK   3      T13:  -0.1569 T23:   0.0838                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.1912 L22:   5.3524                                     
REMARK   3      L33:   0.9439 L12:   2.4452                                     
REMARK   3      L13:  -0.7669 L23:  -1.7978                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0395 S12:  -0.2404 S13:  -0.2795                       
REMARK   3      S21:   0.3258 S22:  -0.0987 S23:  -0.3469                       
REMARK   3      S31:   0.1551 S32:   0.1645 S33:   0.0812                       
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    SELECTION: ( CHAIN D AND RESID 267:331 )                          
REMARK   3    ORIGIN FOR THE GROUP (A):   24.519   50.268  -13.832              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2467 T22:   0.2984                                     
REMARK   3      T33:   0.5423 T12:   0.0739                                     
REMARK   3      T13:  -0.0608 T23:  -0.0639                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.1305 L22:   4.2123                                     
REMARK   3      L33:   0.4568 L12:   3.7240                                     
REMARK   3      L13:   0.7319 L23:   0.4506                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2021 S12:   0.2246 S13:  -0.3107                       
REMARK   3      S21:  -0.1343 S22:   0.2390 S23:  -0.5756                       
REMARK   3      S31:  -0.0116 S32:   0.1293 S33:  -0.0011                       
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    SELECTION: ( CHAIN G AND RESID 1:5 )                              
REMARK   3    ORIGIN FOR THE GROUP (A):   31.787   12.387   -6.615              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8439 T22:   1.0048                                     
REMARK   3      T33:   1.5067 T12:   0.5770                                     
REMARK   3      T13:  -0.0194 T23:   0.0209                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.1351 L22:   0.3160                                     
REMARK   3      L33:   0.6230 L12:   0.1118                                     
REMARK   3      L13:   0.1875 L23:  -0.1295                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0212 S12:   0.0429 S13:  -0.3443                       
REMARK   3      S21:   0.0183 S22:   0.0112 S23:  -0.3105                       
REMARK   3      S31:   0.3962 S32:   0.2404 S33:  -0.0232                       
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    SELECTION: ( CHAIN G AND RESID 6:10 )                             
REMARK   3    ORIGIN FOR THE GROUP (A):   20.622   25.306  -11.898              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3782 T22:   0.5492                                     
REMARK   3      T33:   0.8957 T12:   0.2145                                     
REMARK   3      T13:  -0.0888 T23:   0.0407                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.8601 L22:   0.9593                                     
REMARK   3      L33:   1.3032 L12:  -0.7648                                     
REMARK   3      L13:   0.3275 L23:  -0.8645                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1597 S12:  -0.1137 S13:   0.3543                       
REMARK   3      S21:   0.0886 S22:  -0.1496 S23:  -0.4968                       
REMARK   3      S31:   0.0302 S32:   0.4814 S33:   0.1402                       
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    SELECTION: ( CHAIN G AND RESID 11:19 )                            
REMARK   3    ORIGIN FOR THE GROUP (A):   -0.484   33.814   -6.864              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4484 T22:   0.3090                                     
REMARK   3      T33:   0.3882 T12:   0.1302                                     
REMARK   3      T13:  -0.0031 T23:   0.0473                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.6275 L22:   3.3950                                     
REMARK   3      L33:   0.4670 L12:  -3.7042                                     
REMARK   3      L13:  -1.0760 L23:   0.5556                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.5827 S12:  -0.5207 S13:  -0.4100                       
REMARK   3      S21:   0.7581 S22:   0.2958 S23:  -0.2130                       
REMARK   3      S31:   0.4698 S32:   0.0506 S33:   0.2801                       
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    SELECTION: ( CHAIN H AND RESID 1:10 )                             
REMARK   3    ORIGIN FOR THE GROUP (A):    1.932   32.496   -8.146              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4036 T22:   0.3119                                     
REMARK   3      T33:   0.4829 T12:   0.1499                                     
REMARK   3      T13:  -0.0091 T23:   0.0251                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.8262 L22:   2.2412                                     
REMARK   3      L33:   1.3855 L12:  -1.2211                                     
REMARK   3      L13:  -0.0581 L23:  -0.6931                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0287 S12:   0.0640 S13:  -0.7860                       
REMARK   3      S21:   0.2616 S22:  -0.0331 S23:   0.0933                       
REMARK   3      S31:   0.3149 S32:   0.1227 S33:   0.0100                       
REMARK   3   TLS GROUP : 14                                                     
REMARK   3    SELECTION: ( CHAIN H AND RESID 11:19 )                            
REMARK   3    ORIGIN FOR THE GROUP (A):   27.948   17.715  -11.055              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.6001 T22:   0.8048                                     
REMARK   3      T33:   1.1939 T12:   0.4158                                     
REMARK   3      T13:  -0.0358 T23:   0.0283                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.5261 L22:   0.0011                                     
REMARK   3      L33:   0.3118 L12:  -0.0239                                     
REMARK   3      L13:  -0.6900 L23:   0.0118                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0826 S12:   0.1801 S13:  -0.2705                       
REMARK   3      S21:   0.0905 S22:  -0.0670 S23:  -0.4536                       
REMARK   3      S31:   0.3664 S32:   0.6129 S33:   0.0926                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : 3                                           
REMARK   3   NCS GROUP : 1                                                      
REMARK   3    NCS OPERATOR : 1                                                  
REMARK   3     REFERENCE SELECTION: (CHAIN A AND ((RESID 154 THROUGH 158 AND    
REMARK   3                          (NAME N OR NAME CA OR NAME C OR NAME O OR   
REMARK   3                          NAME CB )) OR RESID 159 THROUGH 163 OR      
REMARK   3                          (RESID 164 AND (NAME N OR NAME CA OR NAME   
REMARK   3                          C OR NAME O OR NAME CB )) OR RESID 165      
REMARK   3                          THROUGH 171 OR RESID 174 THROUGH 175 OR     
REMARK   3                          (RESID 176 AND (NAME N OR NAME CA OR NAME   
REMARK   3                          C OR NAME O OR NAME CB )) OR RESID 177      
REMARK   3                          THROUGH 181 OR RESID 183 THROUGH 198 OR     
REMARK   3                          RESID 200 THROUGH 202 OR (RESID 203 AND     
REMARK   3                          (NAME N OR NAME CA OR NAME C OR NAME O OR   
REMARK   3                          NAME CB )) OR RESID 204 THROUGH 217))       
REMARK   3     SELECTION          : (CHAIN C AND (RESID 154 THROUGH 171 OR      
REMARK   3                          RESID 174 THROUGH 181 OR RESID 183          
REMARK   3                          THROUGH 198 OR RESID 200 THROUGH 217))      
REMARK   3     ATOM PAIRS NUMBER  : 636                                         
REMARK   3     RMSD               : NULL                                        
REMARK   3   NCS GROUP : 2                                                      
REMARK   3    NCS OPERATOR : 1                                                  
REMARK   3     REFERENCE SELECTION: (CHAIN E AND (RESID 1 THROUGH 9 OR RESID    
REMARK   3                          11 THROUGH 18))                             
REMARK   3     SELECTION          : (CHAIN F AND ((RESID 23 AND (NAME C4 OR     
REMARK   3                          NAME O4 OR NAME C3 OR NAME O3 OR NAME C2    
REMARK   3                          OR NAME C1 OR NAME N1 OR NAME C2 OR NAME    
REMARK   3                          O2 OR NAME N3 OR NAME C4 OR NAME N4 OR      
REMARK   3                          NAME C5 OR NAME C6 )) OR RESID 24 THROUGH   
REMARK   3                          31 OR RESID 33 THROUGH 40))                 
REMARK   3     ATOM PAIRS NUMBER  : 706                                         
REMARK   3     RMSD               : NULL                                        
REMARK   3    NCS OPERATOR : 2                                                  
REMARK   3     REFERENCE SELECTION: (CHAIN E AND (RESID 1 THROUGH 9 OR RESID    
REMARK   3                          11 THROUGH 18))                             
REMARK   3     SELECTION          : (CHAIN G AND (RESID 1 THROUGH 9 OR RESID    
REMARK   3                          11 THROUGH 18))                             
REMARK   3     ATOM PAIRS NUMBER  : 706                                         
REMARK   3     RMSD               : NULL                                        
REMARK   3    NCS OPERATOR : 3                                                  
REMARK   3     REFERENCE SELECTION: (CHAIN E AND (RESID 1 THROUGH 9 OR RESID    
REMARK   3                          11 THROUGH 18))                             
REMARK   3     SELECTION          : (CHAIN H AND ((RESID 23 AND (NAME C4 OR     
REMARK   3                          NAME O4 OR NAME C3 OR NAME O3 OR NAME C2    
REMARK   3                          OR NAME C1 OR NAME N1 OR NAME C2 OR NAME    
REMARK   3                          O2 OR NAME N3 OR NAME C4 OR NAME N4 OR      
REMARK   3                          NAME C5 OR NAME C6 )) OR RESID 24 THROUGH   
REMARK   3                          31 OR RESID 33 THROUGH 40))                 
REMARK   3     ATOM PAIRS NUMBER  : 706                                         
REMARK   3     RMSD               : NULL                                        
REMARK   3   NCS GROUP : 3                                                      
REMARK   3    NCS OPERATOR : 1                                                  
REMARK   3     REFERENCE SELECTION: (CHAIN B AND (RESID 267 THROUGH 269 OR      
REMARK   3                          (RESID 270 THROUGH 271 AND (NAME N OR       
REMARK   3                          NAME CA OR NAME C OR NAME O OR NAME CB ))   
REMARK   3                          OR RESID 272 THROUGH 278 OR RESID 280       
REMARK   3                          THROUGH 285 OR RESID 287 OR RESID 289       
REMARK   3                          THROUGH 307 OR (RESID 309 AND (NAME N OR    
REMARK   3                          NAME CA OR NAME C OR NAME O OR NAME CB ))   
REMARK   3                          OR RESID 310 THROUGH 325 OR (RESID 326      
REMARK   3                          AND (NAME N OR NAME CA OR NAME C OR NAME    
REMARK   3                          O OR NAME CB )) OR RESID 327 THROUGH 331))  
REMARK   3     SELECTION          : (CHAIN D AND ((RESID 267 THROUGH 268 AND    
REMARK   3                          (NAME N OR NAME CA OR NAME C OR NAME O OR   
REMARK   3                          NAME CB )) OR RESID 269 THROUGH 278 OR      
REMARK   3                          RESID 280 THROUGH 285 OR RESID 287 OR       
REMARK   3                          RESID 289 THROUGH 307 OR RESID 309          
REMARK   3                          THROUGH 331))                               
REMARK   3     ATOM PAIRS NUMBER  : 662                                         
REMARK   3     RMSD               : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5VPF COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 05-MAY-17.                  
REMARK 100 THE DEPOSITION ID IS D_1000227771.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 16-JUL-16                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : APS                                
REMARK 200  BEAMLINE                       : 19-ID                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.979                              
REMARK 200  MONOCHROMATOR                  : SI(111)                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M                 
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 30141                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.700                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 15.70                              
REMARK 200  R MERGE                    (I) : 0.10700                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 8.8000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.70                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.75                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 15.50                              
REMARK 200  R MERGE FOR SHELL          (I) : 1.75200                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 1FOS                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 73.41                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 4.63                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.9 M NA2HPO4, PH 5.5, VAPOR             
REMARK 280  DIFFUSION, HANGING DROP, TEMPERATURE 293K                           
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 61 2 2                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+1/3                                            
REMARK 290       3555   -X+Y,-X,Z+2/3                                           
REMARK 290       4555   -X,-Y,Z+1/2                                             
REMARK 290       5555   Y,-X+Y,Z+5/6                                            
REMARK 290       6555   X-Y,X,Z+1/6                                             
REMARK 290       7555   Y,X,-Z+1/3                                              
REMARK 290       8555   X-Y,-Y,-Z                                               
REMARK 290       9555   -X,-X+Y,-Z+2/3                                          
REMARK 290      10555   -Y,-X,-Z+5/6                                            
REMARK 290      11555   -X+Y,Y,-Z+1/2                                           
REMARK 290      12555   X,X-Y,-Z+1/6                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       83.32333            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000      166.64667            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000      124.98500            
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000      208.30833            
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       41.66167            
REMARK 290   SMTRY1   7 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   7  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       83.32333            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   9 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   9 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   9  0.000000  0.000000 -1.000000      166.64667            
REMARK 290   SMTRY1  10  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  10 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  10  0.000000  0.000000 -1.000000      208.30833            
REMARK 290   SMTRY1  11 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2  11  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3  11  0.000000  0.000000 -1.000000      124.98500            
REMARK 290   SMTRY1  12  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  12  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  12  0.000000  0.000000 -1.000000       41.66167            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 8710 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 14840 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -74.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, E, F                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7370 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 15020 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -59.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D, G, H                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     LYS A   219                                                      
REMARK 465     SER B   265                                                      
REMARK 465     SER C   152                                                      
REMARK 465     GLU C   153                                                      
REMARK 465     HIS C   218                                                      
REMARK 465     LYS C   219                                                      
REMARK 465     SER D   265                                                      
REMARK 465     GLN D   266                                                      
REMARK 465     VAL D   332                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLU A 155    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 157    CG   CD   CE   NZ                                   
REMARK 470     HIS A 218    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLN B 266    CG   CD   OE1  NE2                                  
REMARK 470     GLU B 267    CG   CD   OE1  OE2                                  
REMARK 470     ARG B 268    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470      DC E   1    O5'  C5'                                            
REMARK 470      DG F  19    C5'  C4'  O4'  C3'  O3'  C2'  C1'                   
REMARK 470      DG F  19    N9   C8   N7   C5   C6   O6   N1                    
REMARK 470      DG F  19    C2   N2   N3   C4                                   
REMARK 470     GLU C 154    CG   CD   OE1  OE2                                  
REMARK 470     GLU C 155    CG   CD   OE1  OE2                                  
REMARK 470     GLU C 156    CG   CD   OE1  OE2                                  
REMARK 470     LYS C 157    CG   CD   CE   NZ                                   
REMARK 470     ARG C 158    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG C 164    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG C 176    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU C 203    CG   CD   OE1  OE2                                  
REMARK 470     ARG D 268    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS D 270    CG   CD   CE   NZ                                   
REMARK 470     GLU D 309    CG   CD   OE1  OE2                                  
REMARK 470     GLN D 326    CG   CD   OE1  NE2                                  
REMARK 470      DC G   1    O5'  C5'                                            
REMARK 470      DC H   1    O5'                                                 
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   H42   DC E     1     O6    DG H    19    10564     1.57            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500     DG F   2   O3'    DG F   2   C3'    -0.040                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500     DC E   1   O4' -  C1' -  N1  ANGL. DEV. =   2.1 DEGREES          
REMARK 500     DC G  18   O4' -  C1' -  N1  ANGL. DEV. =   2.5 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ALA A 217       31.77    -81.89                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 301                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 302                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 303                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 304                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO B 401                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue CL B 402                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC7                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue NA B 403                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC8                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO E 101                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC9                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO F 101                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 301                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 C 302                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO D 401                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue PO4 G 101                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5VPA   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPB   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPC   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPD   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 5VPE   RELATED DB: PDB                                   
DBREF  5VPF A  153   219  UNP    P53539   FOSB_HUMAN     153    219             
DBREF  5VPF B  266   332  UNP    P17535   JUND_HUMAN     266    332             
DBREF  5VPF E    1    19  PDB    5VPF     5VPF             1     19             
DBREF  5VPF F    1    19  PDB    5VPF     5VPF             1     19             
DBREF  5VPF C  153   219  UNP    P53539   FOSB_HUMAN     153    219             
DBREF  5VPF D  266   332  UNP    P17535   JUND_HUMAN     266    332             
DBREF  5VPF G    1    19  PDB    5VPF     5VPF             1     19             
DBREF  5VPF H    1    19  PDB    5VPF     5VPF             1     19             
SEQADV 5VPF SER A  152  UNP  P53539              EXPRESSION TAG                 
SEQADV 5VPF SER B  265  UNP  P17535              EXPRESSION TAG                 
SEQADV 5VPF SER C  152  UNP  P53539              EXPRESSION TAG                 
SEQADV 5VPF SER D  265  UNP  P17535              EXPRESSION TAG                 
SEQRES   1 A   68  SER GLU GLU GLU GLU LYS ARG ARG VAL ARG ARG GLU ARG          
SEQRES   2 A   68  ASN LYS LEU ALA ALA ALA LYS CYS ARG ASN ARG ARG ARG          
SEQRES   3 A   68  GLU LEU THR ASP ARG LEU GLN ALA GLU THR ASP GLN LEU          
SEQRES   4 A   68  GLU GLU GLU LYS ALA GLU LEU GLU SER GLU ILE ALA GLU          
SEQRES   5 A   68  LEU GLN LYS GLU LYS GLU ARG LEU GLU PHE VAL LEU VAL          
SEQRES   6 A   68  ALA HIS LYS                                                  
SEQRES   1 B   68  SER GLN GLU ARG ILE LYS ALA GLU ARG LYS ARG LEU ARG          
SEQRES   2 B   68  ASN ARG ILE ALA ALA SER LYS CYS ARG LYS ARG LYS LEU          
SEQRES   3 B   68  GLU ARG ILE SER ARG LEU GLU GLU LYS VAL LYS THR LEU          
SEQRES   4 B   68  LYS SER GLN ASN THR GLU LEU ALA SER THR ALA SER LEU          
SEQRES   5 B   68  LEU ARG GLU GLN VAL ALA GLN LEU LYS GLN LYS VAL LEU          
SEQRES   6 B   68  SER HIS VAL                                                  
SEQRES   1 E   19   DC  DG  DT  DC  DG  DG  DT  DG  DA  DC  DT  DC  DA          
SEQRES   2 E   19   DC  DC  DG  DA  DC  DG                                      
SEQRES   1 F   19   DC  DG  DT  DC  DG  DG  DT  DG  DA  DG  DT  DC  DA          
SEQRES   2 F   19   DC  DC  DG  DA  DC  DG                                      
SEQRES   1 C   68  SER GLU GLU GLU GLU LYS ARG ARG VAL ARG ARG GLU ARG          
SEQRES   2 C   68  ASN LYS LEU ALA ALA ALA LYS CYS ARG ASN ARG ARG ARG          
SEQRES   3 C   68  GLU LEU THR ASP ARG LEU GLN ALA GLU THR ASP GLN LEU          
SEQRES   4 C   68  GLU GLU GLU LYS ALA GLU LEU GLU SER GLU ILE ALA GLU          
SEQRES   5 C   68  LEU GLN LYS GLU LYS GLU ARG LEU GLU PHE VAL LEU VAL          
SEQRES   6 C   68  ALA HIS LYS                                                  
SEQRES   1 D   68  SER GLN GLU ARG ILE LYS ALA GLU ARG LYS ARG LEU ARG          
SEQRES   2 D   68  ASN ARG ILE ALA ALA SER LYS CYS ARG LYS ARG LYS LEU          
SEQRES   3 D   68  GLU ARG ILE SER ARG LEU GLU GLU LYS VAL LYS THR LEU          
SEQRES   4 D   68  LYS SER GLN ASN THR GLU LEU ALA SER THR ALA SER LEU          
SEQRES   5 D   68  LEU ARG GLU GLN VAL ALA GLN LEU LYS GLN LYS VAL LEU          
SEQRES   6 D   68  SER HIS VAL                                                  
SEQRES   1 G   19   DC  DG  DT  DC  DG  DG  DT  DG  DA  DC  DT  DC  DA          
SEQRES   2 G   19   DC  DC  DG  DA  DC  DG                                      
SEQRES   1 H   19   DC  DG  DT  DC  DG  DG  DT  DG  DA  DG  DT  DC  DA          
SEQRES   2 H   19   DC  DC  DG  DA  DC  DG                                      
HET    EDO  A 301      10                                                       
HET    EDO  A 302      10                                                       
HET    EDO  A 303      10                                                       
HET     CL  A 304       1                                                       
HET    EDO  B 401      10                                                       
HET     CL  B 402       1                                                       
HET     NA  B 403       1                                                       
HET    EDO  E 101      10                                                       
HET    EDO  F 101      10                                                       
HET    EDO  C 301      10                                                       
HET    PO4  C 302       5                                                       
HET    EDO  D 401      10                                                       
HET    PO4  G 101       5                                                       
HETNAM     EDO 1,2-ETHANEDIOL                                                   
HETNAM      CL CHLORIDE ION                                                     
HETNAM      NA SODIUM ION                                                       
HETNAM     PO4 PHOSPHATE ION                                                    
HETSYN     EDO ETHYLENE GLYCOL                                                  
FORMUL   9  EDO    8(C2 H6 O2)                                                  
FORMUL  12   CL    2(CL 1-)                                                     
FORMUL  15   NA    NA 1+                                                        
FORMUL  19  PO4    2(O4 P 3-)                                                   
FORMUL  22  HOH   *71(H2 O)                                                     
HELIX    1 AA1 GLU A  153  ALA A  217  1                                  65    
HELIX    2 AA2 GLU B  267  SER B  330  1                                  64    
HELIX    3 AA3 GLU C  156  VAL C  216  1                                  61    
HELIX    4 AA4 ARG D  268  SER D  330  1                                  63    
SITE     1 AC1  4 ARG A 173  ARG B 286   DA F   9  EDO F 101                    
SITE     1 AC2  3 GLU A 191  LYS A 194  LYS B 299                               
SITE     1 AC3  2 GLN A 184  ASP A 188                                          
SITE     1 AC4  4 ARG A 161  ARG A 164   DC E   4   DG E   5                    
SITE     1 AC5  3 GLU A 212  ARG B 279  SER B 283                               
SITE     1 AC6  5 ASN B 278  ALA B 281   DG F   5   DG F   6                    
SITE     2 AC6  5 HOH F 208                                                     
SITE     1 AC7  1 SER B 283                                                     
SITE     1 AC8  2  DA E  17   DC E  18                                          
SITE     1 AC9  9 EDO A 301  ALA B 282  CYS B 285  ARG B 286                    
SITE     2 AC9  9 LYS B 289   DT E  11   DT F   7   DG F   8                    
SITE     3 AC9  9  DA F   9                                                     
SITE     1 AD1  4 ARG C 173  ARG D 286   DG H   8   DA H   9                    
SITE     1 AD2  5 GLN C 184  ASP C 188  GLU C 191  LEU D 296                    
SITE     2 AD2  5 LYS D 299                                                     
SITE     1 AD3  4 SER D 283  ARG D 286   DA G   9   DC G  10                    
SITE     1 AD4  3  DG G  16   DA G  17   DC G  18                               
CRYST1  119.637  119.637  249.970  90.00  90.00 120.00 P 61 2 2     24          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.008359  0.004826  0.000000        0.00000                         
SCALE2      0.000000  0.009652  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.004000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system