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Database: PDB
Entry: 6G6J
LinkDB: 6G6J
Original site: 6G6J 
HEADER    APOPTOSIS                               01-APR-18   6G6J              
TITLE     THE CRYSTAL STRUCTURES OF HUMAN MYC:MAX BHLHZIP COMPLEX               
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: MYC PROTO-ONCOGENE PROTEIN;                                
COMPND   3 CHAIN: A, C;                                                         
COMPND   4 SYNONYM: CLASS E BASIC HELIX-LOOP-HELIX PROTEIN 39,BHLHE39,PROTO-    
COMPND   5 ONCOGENE C-MYC,TRANSCRIPTION FACTOR P64;                             
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: PROTEIN MAX;                                               
COMPND   9 CHAIN: B, D;                                                         
COMPND  10 SYNONYM: CLASS D BASIC HELIX-LOOP-HELIX PROTEIN 4,BHLHD4,MYC-        
COMPND  11 ASSOCIATED FACTOR X;                                                 
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: MYC, BHLHE39;                                                  
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  10 ORGANISM_COMMON: HUMAN;                                              
SOURCE  11 ORGANISM_TAXID: 9606;                                                
SOURCE  12 GENE: MAX, BHLHD4;                                                   
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    MYC/MAX, APOPTOSIS                                                    
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    M.D.ALLEN,G.ZINZALLA                                                  
REVDAT   3   31-JUL-19 6G6J    1       JRNL                                     
REVDAT   2   24-JUL-19 6G6J    1       JRNL                                     
REVDAT   1   10-APR-19 6G6J    0                                                
JRNL        AUTH   S.SAMMAK,N.HAMDANI,F.GORREC,M.D.ALLEN,S.M.V.FREUND,          
JRNL        AUTH 2 M.BYCROFT,G.ZINZALLA                                         
JRNL        TITL   CRYSTAL STRUCTURES AND NUCLEAR MAGNETIC RESONANCE STUDIES OF 
JRNL        TITL 2 THE APO FORM OF THE C-MYC:MAX BHLHZIP COMPLEX REVEAL A       
JRNL        TITL 3 HELICAL BASIC REGION IN THE ABSENCE OF DNA.                  
JRNL        REF    BIOCHEMISTRY                  V.  58  3144 2019              
JRNL        REFN                   ISSN 0006-2960                               
JRNL        PMID   31260268                                                     
JRNL        DOI    10.1021/ACS.BIOCHEM.9B00296                                  
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.25 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.12_2829: ???)                              
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.25                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 40.55                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.330                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.3                           
REMARK   3   NUMBER OF REFLECTIONS             : 25534                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.207                           
REMARK   3   R VALUE            (WORKING SET) : 0.205                           
REMARK   3   FREE R VALUE                     : 0.236                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.850                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1239                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 40.5602 -  4.6781    0.98     2836   143  0.2110 0.2092        
REMARK   3     2  4.6781 -  3.7139    1.00     2739   142  0.1605 0.1961        
REMARK   3     3  3.7139 -  3.2447    1.00     2727   132  0.1955 0.2390        
REMARK   3     4  3.2447 -  2.9481    1.00     2719   123  0.2164 0.2487        
REMARK   3     5  2.9481 -  2.7369    0.99     2654   151  0.2219 0.2573        
REMARK   3     6  2.7369 -  2.5755    0.99     2660   144  0.2262 0.2786        
REMARK   3     7  2.5755 -  2.4466    0.99     2634   145  0.2240 0.2727        
REMARK   3     8  2.4466 -  2.3401    0.99     2659   130  0.2451 0.2644        
REMARK   3     9  2.3401 -  2.2500    1.00     2667   129  0.2605 0.2945        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.230            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 29.530           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.006           2586                                  
REMARK   3   ANGLE     :  0.677           3455                                  
REMARK   3   CHIRALITY :  0.037            373                                  
REMARK   3   PLANARITY :  0.003            453                                  
REMARK   3   DIHEDRAL  :  2.461           1655                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 6G6J COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 02-APR-18.                  
REMARK 100 THE DEPOSITION ID IS D_1200009481.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 08-AUG-17                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 7                                  
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : DIAMOND                            
REMARK 200  BEAMLINE                       : I03                                
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.97625                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS3 6M                
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : IMOSFLM                            
REMARK 200  DATA SCALING SOFTWARE          : SCALA                              
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 25627                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.250                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 51.930                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.7                               
REMARK 200  DATA REDUNDANCY                : 4.800                              
REMARK 200  R MERGE                    (I) : 0.09800                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 8.9000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.25                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.37                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.7                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 4.90                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.54500                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.800                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 1KNP                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 59.74                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.06                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 20% (W/V) PEG 3350 0.2M SODIUM SULFATE   
REMARK 280  DECAHYDRATE, PH 7, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE       
REMARK 280  290K                                                                
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z                                                 
REMARK 290       3555   -X+1/2,Y+1/2,-Z                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       37.15950            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       72.59000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       37.15950            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       72.59000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 4000 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 10720 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -68.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 3510 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 10440 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -47.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C, D                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A   891                                                      
REMARK 465     HIS A   892                                                      
REMARK 465     HIS A   893                                                      
REMARK 465     HIS A   894                                                      
REMARK 465     HIS A   895                                                      
REMARK 465     HIS A   896                                                      
REMARK 465     HIS A   897                                                      
REMARK 465     GLU A   898                                                      
REMARK 465     GLU A   899                                                      
REMARK 465     ASN A   900                                                      
REMARK 465     VAL A   901                                                      
REMARK 465     LYS A   902                                                      
REMARK 465     ARG A   903                                                      
REMARK 465     ARG A   904                                                      
REMARK 465     MET B   200                                                      
REMARK 465     ALA B   201                                                      
REMARK 465     ASP B   202                                                      
REMARK 465     LYS B   203                                                      
REMARK 465     ARG B   204                                                      
REMARK 465     ALA B   205                                                      
REMARK 465     HIS B   206                                                      
REMARK 465     HIS B   207                                                      
REMARK 465     ASN B   208                                                      
REMARK 465     GLU B   282                                                      
REMARK 465     MET C   891                                                      
REMARK 465     HIS C   892                                                      
REMARK 465     HIS C   893                                                      
REMARK 465     HIS C   894                                                      
REMARK 465     HIS C   895                                                      
REMARK 465     HIS C   896                                                      
REMARK 465     HIS C   897                                                      
REMARK 465     GLU C   898                                                      
REMARK 465     GLU C   899                                                      
REMARK 465     ASN C   900                                                      
REMARK 465     VAL C   901                                                      
REMARK 465     LYS C   902                                                      
REMARK 465     ARG C   903                                                      
REMARK 465     ARG C   904                                                      
REMARK 465     THR C   905                                                      
REMARK 465     HIS C   906                                                      
REMARK 465     MET D   200                                                      
REMARK 465     ALA D   201                                                      
REMARK 465     ASP D   202                                                      
REMARK 465     LYS D   203                                                      
REMARK 465     ARG D   204                                                      
REMARK 465     ALA D   205                                                      
REMARK 465     HIS D   206                                                      
REMARK 465     HIS D   207                                                      
REMARK 465     ASN D   208                                                      
REMARK 465     ALA D   209                                                      
REMARK 465     LEU D   210                                                      
REMARK 465     GLU D   211                                                      
REMARK 465     GLU D   282                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    HOH B   412     O    HOH B   437              1.81            
REMARK 500   O    HOH B   418     O    HOH B   420              1.96            
REMARK 500   O2   SO4 A  1002     O    HOH A  1101              1.99            
REMARK 500   OD2  ASP D   227     O    HOH D   401              2.03            
REMARK 500   NH1  ARG C   968     O    HOH C  1101              2.03            
REMARK 500   O    GLN B   233     O    HOH B   401              2.08            
REMARK 500   NE   ARG C   982     O    HOH C  1102              2.10            
REMARK 500   OD2  ASP B   227     O    HOH B   402              2.12            
REMARK 500   OE2  GLU A   916     NH2  ARG A   919              2.14            
REMARK 500   OE1  GLU A   916     O    HOH A  1102              2.15            
REMARK 500   O    HOH D   423     O    HOH D   435              2.17            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   O    HOH C  1135     O    HOH C  1144     2665     2.16            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN A 933       19.06     50.75                                   
REMARK 500    ASN A 934      100.08    -59.94                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 1001                
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 A 1002                
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 B 301                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 B 302                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 C 1001                
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue SO4 D 301                 
DBREF  6G6J A  898   984  UNP    P01106   MYC_HUMAN      351    437             
DBREF  6G6J B  201   282  UNP    P61244   MAX_HUMAN       22    103             
DBREF  6G6J C  898   984  UNP    P01106   MYC_HUMAN      351    437             
DBREF  6G6J D  201   282  UNP    P61244   MAX_HUMAN       22    103             
SEQADV 6G6J MET A  891  UNP  P01106              INITIATING METHIONINE          
SEQADV 6G6J HIS A  892  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS A  893  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS A  894  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS A  895  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS A  896  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS A  897  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J MET B  200  UNP  P61244              INITIATING METHIONINE          
SEQADV 6G6J MET C  891  UNP  P01106              INITIATING METHIONINE          
SEQADV 6G6J HIS C  892  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS C  893  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS C  894  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS C  895  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS C  896  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J HIS C  897  UNP  P01106              EXPRESSION TAG                 
SEQADV 6G6J MET D  200  UNP  P61244              INITIATING METHIONINE          
SEQRES   1 A   94  MET HIS HIS HIS HIS HIS HIS GLU GLU ASN VAL LYS ARG          
SEQRES   2 A   94  ARG THR HIS ASN VAL LEU GLU ARG GLN ARG ARG ASN GLU          
SEQRES   3 A   94  LEU LYS ARG SER PHE PHE ALA LEU ARG ASP GLN ILE PRO          
SEQRES   4 A   94  GLU LEU GLU ASN ASN GLU LYS ALA PRO LYS VAL VAL ILE          
SEQRES   5 A   94  LEU LYS LYS ALA THR ALA TYR ILE LEU SER VAL GLN ALA          
SEQRES   6 A   94  GLU GLU GLN LYS LEU ILE SER GLU GLU ASP LEU LEU ARG          
SEQRES   7 A   94  LYS ARG ARG GLU GLN LEU LYS HIS LYS LEU GLU GLN LEU          
SEQRES   8 A   94  ARG ASN SER                                                  
SEQRES   1 B   83  MET ALA ASP LYS ARG ALA HIS HIS ASN ALA LEU GLU ARG          
SEQRES   2 B   83  LYS ARG ARG ASP HIS ILE LYS ASP SER PHE HIS SER LEU          
SEQRES   3 B   83  ARG ASP SER VAL PRO SER LEU GLN GLY GLU LYS ALA SER          
SEQRES   4 B   83  ARG ALA GLN ILE LEU ASP LYS ALA THR GLU TYR ILE GLN          
SEQRES   5 B   83  TYR MET ARG ARG LYS ASN HIS THR HIS GLN GLN ASP ILE          
SEQRES   6 B   83  ASP ASP LEU LYS ARG GLN ASN ALA LEU LEU GLU GLN GLN          
SEQRES   7 B   83  VAL ARG ALA LEU GLU                                          
SEQRES   1 C   94  MET HIS HIS HIS HIS HIS HIS GLU GLU ASN VAL LYS ARG          
SEQRES   2 C   94  ARG THR HIS ASN VAL LEU GLU ARG GLN ARG ARG ASN GLU          
SEQRES   3 C   94  LEU LYS ARG SER PHE PHE ALA LEU ARG ASP GLN ILE PRO          
SEQRES   4 C   94  GLU LEU GLU ASN ASN GLU LYS ALA PRO LYS VAL VAL ILE          
SEQRES   5 C   94  LEU LYS LYS ALA THR ALA TYR ILE LEU SER VAL GLN ALA          
SEQRES   6 C   94  GLU GLU GLN LYS LEU ILE SER GLU GLU ASP LEU LEU ARG          
SEQRES   7 C   94  LYS ARG ARG GLU GLN LEU LYS HIS LYS LEU GLU GLN LEU          
SEQRES   8 C   94  ARG ASN SER                                                  
SEQRES   1 D   83  MET ALA ASP LYS ARG ALA HIS HIS ASN ALA LEU GLU ARG          
SEQRES   2 D   83  LYS ARG ARG ASP HIS ILE LYS ASP SER PHE HIS SER LEU          
SEQRES   3 D   83  ARG ASP SER VAL PRO SER LEU GLN GLY GLU LYS ALA SER          
SEQRES   4 D   83  ARG ALA GLN ILE LEU ASP LYS ALA THR GLU TYR ILE GLN          
SEQRES   5 D   83  TYR MET ARG ARG LYS ASN HIS THR HIS GLN GLN ASP ILE          
SEQRES   6 D   83  ASP ASP LEU LYS ARG GLN ASN ALA LEU LEU GLU GLN GLN          
SEQRES   7 D   83  VAL ARG ALA LEU GLU                                          
HET    SO4  A1001       5                                                       
HET    SO4  A1002       5                                                       
HET    SO4  B 301       5                                                       
HET    SO4  B 302       5                                                       
HET    SO4  C1001       5                                                       
HET    SO4  D 301       5                                                       
HETNAM     SO4 SULFATE ION                                                      
FORMUL   5  SO4    6(O4 S 2-)                                                   
FORMUL  11  HOH   *180(H2 O)                                                    
HELIX    1 AA1 THR A  905  ASP A  926  1                                  22    
HELIX    2 AA2 PRO A  938  ASN A  983  1                                  46    
HELIX    3 AA3 LEU B  210  VAL B  229  1                                  20    
HELIX    4 AA4 PRO B  230  GLN B  233  5                                   4    
HELIX    5 AA5 SER B  238  ALA B  280  1                                  43    
HELIX    6 AA6 VAL C  908  ASP C  926  1                                  19    
HELIX    7 AA7 PRO C  938  ASN C  983  1                                  46    
HELIX    8 AA8 LYS D  213  VAL D  229  1                                  17    
HELIX    9 AA9 PRO D  230  GLN D  233  5                                   4    
HELIX   10 AB1 SER D  238  LEU D  281  1                                  44    
SITE     1 AC1  2 ASN A 915  ARG A 919                                          
SITE     1 AC2  5 PRO A 938  LYS A 939  HOH A1101  HOH A1118                    
SITE     2 AC2  5 ARG B 214                                                     
SITE     1 AC3  3 SER B 238  ARG B 239  HOH B 403                               
SITE     1 AC4  3 ARG B 254  HIS B 258  HOH B 404                               
SITE     1 AC5  4 LYS C 936  PRO C 938  LYS C 939  HOH C1132                    
SITE     1 AC6  3 SER D 238  ARG D 239  HOH D 403                               
CRYST1   74.319  145.180   48.499  90.00  90.00  90.00 P 21 21 2     8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.013455  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.006888  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.020619        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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