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Database: PDB
Entry: 6GK2
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HEADER    IMMUNE SYSTEM                           18-MAY-18   6GK2              
TITLE     HELICAL RECONSTRUCTION OF BCL10 CARD AND MALT1 DEATH DOMAIN COMPLEX   
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: B-CELL LYMPHOMA/LEUKEMIA 10;                               
COMPND   3 CHAIN: H;                                                            
COMPND   4 SYNONYM: B-CELL CLL/LYMPHOMA 10,BCL-10,CARD-CONTAINING MOLECULE      
COMPND   5 ENHANCING NF-KAPPA-B,CARD-LIKE APOPTOTIC PROTEIN,HCLAP,CED-3/ICH-1   
COMPND   6 PRODOMAIN HOMOLOGOUS E10-LIKE REGULATOR,CIPER,CELLULAR HOMOLOG OF    
COMPND   7 VCARMEN,CCARMEN,CELLULAR-E10,C-E10,MAMMALIAN CARD-CONTAINING ADAPTER 
COMPND   8 MOLECULE E10,ME10;                                                   
COMPND   9 ENGINEERED: YES;                                                     
COMPND  10 MOL_ID: 2;                                                           
COMPND  11 MOLECULE: MUCOSA-ASSOCIATED LYMPHOID TISSUE LYMPHOMA TRANSLOCATION   
COMPND  12 PROTEIN 1;                                                           
COMPND  13 CHAIN: F;                                                            
COMPND  14 SYNONYM: MALT LYMPHOMA-ASSOCIATED TRANSLOCATION,PARACASPASE;         
COMPND  15 EC: 3.4.22.-;                                                        
COMPND  16 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: BCL10, CIPER, CLAP;                                            
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  10 ORGANISM_COMMON: HUMAN;                                              
SOURCE  11 ORGANISM_TAXID: 9606;                                                
SOURCE  12 GENE: MALT1, MLT;                                                    
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    BCL10, MALT1, CBM COMPLEX, HELICAL RECONSTRUCTION, CANCER, AUTOIMMUNE 
KEYWDS   2 DISEASE, IMMUNE SYSTEM                                               
EXPDTA    ELECTRON MICROSCOPY                                                   
AUTHOR    F.SCHLAUDERER,A.DESFOSSES,I.GUTSCHE,K.P.HOPFNER,K.LAMMENS             
REVDAT   1   31-OCT-18 6GK2    0                                                
JRNL        AUTH   F.SCHLAUDERER,T.SEEHOLZER,A.DESFOSSES,T.GEHRING,M.STRAUSS,   
JRNL        AUTH 2 K.P.HOPFNER,I.GUTSCHE,D.KRAPPMANN,K.LAMMENS                  
JRNL        TITL   MOLECULAR ARCHITECTURE AND REGULATION OF BCL10-MALT1         
JRNL        TITL 2 FILAMENTS.                                                   
JRNL        REF    NAT COMMUN                    V.   9  4041 2018              
JRNL        REFN                   ESSN 2041-1723                               
JRNL        PMID   30279415                                                     
JRNL        DOI    10.1038/S41467-018-06573-8                                   
REMARK   2                                                                      
REMARK   2 RESOLUTION.    4.90 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : SPRING, CTFFIND, COOT, SPRING, PHENIX     
REMARK   3   RECONSTRUCTION SCHEMA  : NULL                                      
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : NULL                                
REMARK   3   REFINEMENT SPACE             : NULL                                
REMARK   3   REFINEMENT PROTOCOL          : NULL                                
REMARK   3   REFINEMENT TARGET            : NULL                                
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : NULL                                             
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : NULL                           
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : NULL                           
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 4.900                          
REMARK   3   NUMBER OF PARTICLES               : 9600                           
REMARK   3   CTF CORRECTION METHOD             : NONE                           
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL                    
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: NULL                                                  
REMARK   4                                                                      
REMARK   4 6GK2 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 18-MAY-18.                  
REMARK 100 THE DEPOSITION ID IS D_1200010014.                                   
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : HELICAL                           
REMARK 245   SPECIMEN TYPE                  : NULL                              
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : FILAMENT                          
REMARK 245   PARTICLE TYPE                  : HELICAL                           
REMARK 245   NAME OF SAMPLE                 : COMPLEX OF BCL10 CARD AND MALT1   
REMARK 245                                    DEATH DOMAIN                      
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : 1.00                              
REMARK 245   SAMPLE SUPPORT DETAILS         : NULL                              
REMARK 245   SAMPLE VITRIFICATION DETAILS   : NULL                              
REMARK 245   SAMPLE BUFFER                  : NULL                              
REMARK 245   PH                             : 7.50                              
REMARK 245   SAMPLE DETAILS                 : NULL                              
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : NULL                           
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : NULL                           
REMARK 245   TEMPERATURE (KELVIN)              : NULL                           
REMARK 245   MICROSCOPE MODEL                  : FEI TECNAI ARCTICA             
REMARK 245   DETECTOR TYPE                     : FEI FALCON III (4K X 4K)       
REMARK 245   MINIMUM DEFOCUS (NM)              : NULL                           
REMARK 245   MAXIMUM DEFOCUS (NM)              : NULL                           
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   NOMINAL CS                        : NULL                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 99.60                          
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : NULL                           
REMARK 245   CALIBRATED MAGNIFICATION          : NULL                           
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 200                            
REMARK 245   IMAGING DETAILS                   : NULL                           
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1220 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 12980 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -2.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: H, F                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     ARG F    64                                                      
REMARK 465     GLY F    65                                                      
REMARK 465     ARG F    66                                                      
REMARK 465     LEU F    67                                                      
REMARK 465     ARG F    68                                                      
REMARK 465     LEU F    69                                                      
REMARK 465     LEU F   121                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS H  31       30.65    -91.98                                   
REMARK 500    LYS H  90       47.00    -83.89                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-0013   RELATED DB: EMDB                              
REMARK 900 HELICAL RECONSTRUCTION OF BCL10 CARD AND MALT1 DEATH DOMAIN COMPLEX  
DBREF  6GK2 H   10   115  UNP    O95999   BCL10_HUMAN     10    115             
DBREF  6GK2 F   30   121  UNP    Q9UDY8   MALT1_HUMAN     30    121             
SEQRES   1 H  106  GLU GLU ASP LEU THR GLU VAL LYS LYS ASP ALA LEU GLU          
SEQRES   2 H  106  ASN LEU ARG VAL TYR LEU CYS GLU LYS ILE ILE ALA GLU          
SEQRES   3 H  106  ARG HIS PHE ASP HIS LEU ARG ALA LYS LYS ILE LEU SER          
SEQRES   4 H  106  ARG GLU ASP THR GLU GLU ILE SER CYS ARG THR SER SER          
SEQRES   5 H  106  ARG LYS ARG ALA GLY LYS LEU LEU ASP TYR LEU GLN GLU          
SEQRES   6 H  106  ASN PRO LYS GLY LEU ASP THR LEU VAL GLU SER ILE ARG          
SEQRES   7 H  106  ARG GLU LYS THR GLN ASN PHE LEU ILE GLN LYS ILE THR          
SEQRES   8 H  106  ASP GLU VAL LEU LYS LEU ARG ASN ILE LYS LEU GLU HIS          
SEQRES   9 H  106  LEU LYS                                                      
SEQRES   1 F   92  LEU ASN ARG LEU ARG GLU PRO LEU LEU ARG ARG LEU SER          
SEQRES   2 F   92  GLU LEU LEU ASP GLN ALA PRO GLU GLY ARG GLY TRP ARG          
SEQRES   3 F   92  ARG LEU ALA GLU LEU ALA GLY SER ARG GLY ARG LEU ARG          
SEQRES   4 F   92  LEU SER CYS LEU ASP LEU GLU GLN CYS SER LEU LYS VAL          
SEQRES   5 F   92  LEU GLU PRO GLU GLY SER PRO SER LEU CYS LEU LEU LYS          
SEQRES   6 F   92  LEU MET GLY GLU LYS GLY CYS THR VAL THR GLU LEU SER          
SEQRES   7 F   92  ASP PHE LEU GLN ALA MET GLU HIS THR GLU VAL LEU GLN          
SEQRES   8 F   92  LEU                                                          
HELIX    1 AA1 GLU H   10  ASN H   23  1                                  14    
HELIX    2 AA2 LEU H   24  CYS H   29  1                                   6    
HELIX    3 AA3 ALA H   34  PHE H   38  5                                   5    
HELIX    4 AA4 ASP H   39  LYS H   44  1                                   6    
HELIX    5 AA5 SER H   48  CYS H   57  1                                  10    
HELIX    6 AA6 SER H   61  ASP H   70  1                                  10    
HELIX    7 AA7 LYS H   77  GLU H   89  1                                  13    
HELIX    8 AA8 PHE H   94  LYS H  115  1                                  22    
HELIX    9 AA9 ARG F   34  GLN F   47  1                                  14    
HELIX   10 AB1 GLY F   53  GLU F   59  1                                   7    
HELIX   11 AB2 CYS F   71  VAL F   81  1                                  11    
HELIX   12 AB3 SER F   87  GLY F  100  1                                  14    
HELIX   13 AB4 THR F  102  MET F  113  1                                  12    
HELIX   14 AB5 HIS F  115  GLN F  120  1                                   6    
SSBOND   1 CYS F   77    CYS F   91                          1555   1555  2.04  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1                      
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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