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Database: PDB
Entry: 6MG1
LinkDB: 6MG1
Original site: 6MG1 
HEADER    TRANSCRIPTION/DNA                       12-SEP-18   6MG1              
TITLE     C-TERMINAL BZIP DOMAIN OF HUMAN C/EBPBETA WITH 16BP METHYLATED        
TITLE    2 OLIGONUCLEOTIDE CONTAINING CONSENSUS RECOGNITION SEQUENCE-C2 CRYSTAL 
TITLE    3 FORM                                                                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CCAAT/ENHANCER-BINDING PROTEIN BETA;                       
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: C/EBP BETA,LIVER ACTIVATOR PROTEIN,LAP,LIVER-ENRICHED       
COMPND   5 INHIBITORY PROTEIN,LIP,NUCLEAR FACTOR NF-IL6,TRANSCRIPTION FACTOR 5, 
COMPND   6 TCF-5;                                                               
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MOL_ID: 2;                                                           
COMPND   9 MOLECULE: 16-BP METHYLATED OLIGONUCLEOTIDE;                          
COMPND  10 CHAIN: C, D;                                                         
COMPND  11 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: CEBPB, TCF5, PP9092;                                           
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 SYNTHETIC: YES;                                                      
SOURCE  11 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  12 ORGANISM_TAXID: 9606                                                 
KEYWDS    BZIP TRANSCIPTION FACTOR DNA METHYLATION CPA METHYLATION PROTEIN-DNA  
KEYWDS   2 COMPLEX, TRANSCRIPTION, TRANSCRIPTION-DNA COMPLEX                    
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.R.HORTON,X.CHENG,J.YANG                                             
REVDAT   6   01-JAN-20 6MG1    1       REMARK                                   
REVDAT   5   28-AUG-19 6MG1    1       REMARK                                   
REVDAT   4   29-MAY-19 6MG1    1       JRNL                                     
REVDAT   3   06-FEB-19 6MG1    1       SOURCE                                   
REVDAT   2   16-JAN-19 6MG1    1       JRNL                                     
REVDAT   1   12-DEC-18 6MG1    0                                                
JRNL        AUTH   J.YANG,J.R.HORTON,D.WANG,R.REN,J.LI,D.SUN,Y.HUANG,X.ZHANG,   
JRNL        AUTH 2 R.M.BLUMENTHAL,X.CHENG                                       
JRNL        TITL   STRUCTURAL BASIS FOR EFFECTS OF CPA MODIFICATIONS ON C/EBP   
JRNL        TITL 2 BETA BINDING OF DNA.                                         
JRNL        REF    NUCLEIC ACIDS RES.            V.  47  1774 2019              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   30566668                                                     
JRNL        DOI    10.1093/NAR/GKY1264                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.75 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.14_3260: ???)                              
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.75                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 34.54                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 92.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 33208                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.216                           
REMARK   3   R VALUE            (WORKING SET) : 0.214                           
REMARK   3   FREE R VALUE                     : 0.250                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.960                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1647                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 34.5491 -  4.0046    0.99     2866   154  0.1915 0.2156        
REMARK   3     2  4.0046 -  3.1793    1.00     2854   146  0.1794 0.2362        
REMARK   3     3  3.1793 -  2.7776    0.99     2834   148  0.2194 0.2734        
REMARK   3     4  2.7776 -  2.5237    1.00     2795   146  0.2320 0.2383        
REMARK   3     5  2.5237 -  2.3429    1.00     2868   150  0.2335 0.2648        
REMARK   3     6  2.3429 -  2.2048    1.00     2813   147  0.2322 0.2606        
REMARK   3     7  2.2048 -  2.0944    0.99     2812   146  0.2502 0.3339        
REMARK   3     8  2.0944 -  2.0032    0.99     2777   145  0.2914 0.3026        
REMARK   3     9  2.0032 -  1.9261    0.94     2679   135  0.2943 0.2770        
REMARK   3    10  1.9261 -  1.8597    0.86     2412   125  0.2900 0.3604        
REMARK   3    11  1.8597 -  1.8015    0.74     2111   111  0.3091 0.3225        
REMARK   3    12  1.8015 -  1.7500    0.62     1740    94  0.3683 0.4453        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.260            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 32.880           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.005           1861                                  
REMARK   3   ANGLE     :  0.996           2627                                  
REMARK   3   CHIRALITY :  0.044            290                                  
REMARK   3   PLANARITY :  0.004            236                                  
REMARK   3   DIHEDRAL  : 29.056            752                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 6MG1 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 13-SEP-18.                  
REMARK 100 THE DEPOSITION ID IS D_1000236841.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 04-FEB-18                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : N                                  
REMARK 200  RADIATION SOURCE               : SEALED TUBE                        
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU MICROMAX-003                
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.00000                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU HYPIX-6000HE                
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 35611                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.630                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 34.542                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 80.8                               
REMARK 200  DATA REDUNDANCY                : 10.30                              
REMARK 200  R MERGE                    (I) : 0.08000                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 25.4000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.63                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.69                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.51900                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.200                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHENIX                                                
REMARK 200 STARTING MODEL: 2E42                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 61.14                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.17                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2 M SODIUM CHLORIDE, 0.1 M BIS-TRIS    
REMARK 280  PH 6.5, 25% (W/V) POLYETHYLENE GLYCOL 3350, VAPOR DIFFUSION,        
REMARK 280  SITTING DROP, TEMPERATURE 292K                                      
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       24.05500            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       38.70250            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       24.05500            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       38.70250            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 9500 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 14510 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: 9.0 KCAL/MOL                          
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     HIS A   267                                                      
REMARK 465     MET A   268                                                      
REMARK 465     LEU A   339                                                      
REMARK 465     ALA A   340                                                      
REMARK 465     SER A   341                                                      
REMARK 465     SER A   342                                                      
REMARK 465     GLY A   343                                                      
REMARK 465     HIS A   344                                                      
REMARK 465     HIS B   267                                                      
REMARK 465     MET B   268                                                      
REMARK 465     LEU B   339                                                      
REMARK 465     ALA B   340                                                      
REMARK 465     SER B   341                                                      
REMARK 465     SER B   342                                                      
REMARK 465     GLY B   343                                                      
REMARK 465     HIS B   344                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LYS A 269    CG   CD   CE   NZ                                   
REMARK 470     HIS A 270    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLU A 273    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 279    CG   CD   OE1  OE2                                  
REMARK 470     ASN A 329    CG   OD1  ND2                                       
REMARK 470     LEU A 330    CG   CD1  CD2                                       
REMARK 470     LYS A 332    CG   CD   CE   NZ                                   
REMARK 470     GLN A 333    CG   CD   OE1  NE2                                  
REMARK 470     LEU A 338    CG   CD1  CD2                                       
REMARK 470     LYS B 269    CG   CD   CE   NZ                                   
REMARK 470     HIS B 270    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLU B 273    CG   CD   OE1  OE2                                  
REMARK 470     LEU B 327    CG   CD1  CD2                                       
REMARK 470     ARG B 328    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASN B 329    CG   OD1  ND2                                       
REMARK 470     LEU B 330    CG   CD1  CD2                                       
REMARK 470     LYS B 332    CG   CD   CE   NZ                                   
REMARK 470     GLN B 333    CG   CD   OE1  NE2                                  
REMARK 470     LEU B 334    CG   CD1  CD2                                       
REMARK 470     GLU B 336    CG   CD   OE1  OE2                                  
REMARK 470     LEU B 338    CG   CD1  CD2                                       
REMARK 470      DT C   1    P    OP1  OP2                                       
REMARK 470      DA C  16    C4'  O4'  C3'  O3'  C2'  C1'  N9                    
REMARK 470      DA C  16    C8   N7   C5   C6   N6   N1   C2                    
REMARK 470      DA C  16    N3   C4                                             
REMARK 470      DT D 101    P    OP1  OP2                                       
REMARK 470      DA D 116    C5'  C4'  O4'  C3'  O3'  C2'  C1'                   
REMARK 470      DA D 116    N9   C8   N7   C5   C6   N6   N1                    
REMARK 470      DA D 116    C2   N3   C4                                        
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LYS A 332      -64.79    -25.78                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 PHE A  331     LYS A  332                 -148.62                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 101                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 102                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO D 401                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO D 402                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue GOL D 403                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Di-nucleotide DG C 7 and 5CM C    
REMARK 800  8                                                                   
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC7                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Di-nucleotide 5CM C 8 and DG C    
REMARK 800  9                                                                   
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC8                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Di-nucleotide DG C 9 and 5CM C    
REMARK 800  10                                                                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC9                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Di-nucleotide 5CM C 10 and DA C   
REMARK 800  11                                                                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Di-nucleotide DG D 107 and 5CM    
REMARK 800  D 108                                                               
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Di-nucleotide 5CM D 108 and DG    
REMARK 800  D 109                                                               
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Di-nucleotide DG D 109 and 5CM    
REMARK 800  D 110                                                               
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AD4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for Di-nucleotide 5CM D 110 and DA    
REMARK 800  D 111                                                               
DBREF  6MG1 A  269   344  UNP    P17676   CEBPB_HUMAN    246    321             
DBREF  6MG1 B  269   344  UNP    P17676   CEBPB_HUMAN    246    321             
DBREF  6MG1 C    1    16  PDB    6MG1     6MG1             1     16             
DBREF  6MG1 D  101   116  PDB    6MG1     6MG1           101    116             
SEQADV 6MG1 HIS A  267  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG1 MET A  268  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG1 HIS B  267  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG1 MET B  268  UNP  P17676              EXPRESSION TAG                 
SEQRES   1 A   78  HIS MET LYS HIS SER ASP GLU TYR LYS ILE ARG ARG GLU          
SEQRES   2 A   78  ARG ASN ASN ILE ALA VAL ARG LYS SER ARG ASP LYS ALA          
SEQRES   3 A   78  LYS MET ARG ASN LEU GLU THR GLN HIS LYS VAL LEU GLU          
SEQRES   4 A   78  LEU THR ALA GLU ASN GLU ARG LEU GLN LYS LYS VAL GLU          
SEQRES   5 A   78  GLN LEU SER ARG GLU LEU SER THR LEU ARG ASN LEU PHE          
SEQRES   6 A   78  LYS GLN LEU PRO GLU PRO LEU LEU ALA SER SER GLY HIS          
SEQRES   1 B   78  HIS MET LYS HIS SER ASP GLU TYR LYS ILE ARG ARG GLU          
SEQRES   2 B   78  ARG ASN ASN ILE ALA VAL ARG LYS SER ARG ASP LYS ALA          
SEQRES   3 B   78  LYS MET ARG ASN LEU GLU THR GLN HIS LYS VAL LEU GLU          
SEQRES   4 B   78  LEU THR ALA GLU ASN GLU ARG LEU GLN LYS LYS VAL GLU          
SEQRES   5 B   78  GLN LEU SER ARG GLU LEU SER THR LEU ARG ASN LEU PHE          
SEQRES   6 B   78  LYS GLN LEU PRO GLU PRO LEU LEU ALA SER SER GLY HIS          
SEQRES   1 C   16   DT  DA  DT  DA  DT  DT  DG 5CM  DG 5CM  DA  DA  DT          
SEQRES   2 C   16   DA  DT  DA                                                  
SEQRES   1 D   16   DT  DA  DT  DA  DT  DT  DG 5CM  DG 5CM  DA  DA  DT          
SEQRES   2 D   16   DA  DT  DA                                                  
HET    5CM  C   8      20                                                       
HET    5CM  C  10      20                                                       
HET    5CM  D 108      20                                                       
HET    5CM  D 110      20                                                       
HET    EDO  C 101       4                                                       
HET    EDO  C 102       4                                                       
HET    EDO  D 401       4                                                       
HET    EDO  D 402       4                                                       
HET    GOL  D 403       6                                                       
HETNAM     5CM 5-METHYL-2'-DEOXY-CYTIDINE-5'-MONOPHOSPHATE                      
HETNAM     EDO 1,2-ETHANEDIOL                                                   
HETNAM     GOL GLYCEROL                                                         
HETSYN     EDO ETHYLENE GLYCOL                                                  
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL                                    
FORMUL   3  5CM    4(C10 H16 N3 O7 P)                                           
FORMUL   5  EDO    4(C2 H6 O2)                                                  
FORMUL   9  GOL    C3 H8 O3                                                     
FORMUL  10  HOH   *132(H2 O)                                                    
HELIX    1 AA1 SER A  271  LEU A  330  1                                  60    
HELIX    2 AA2 SER B  271  PHE B  331  1                                  61    
LINK         O3'  DG C   7                 P   5CM C   8     1555   1555  1.60  
LINK         O3' 5CM C   8                 P    DG C   9     1555   1555  1.61  
LINK         O3'  DG C   9                 P   5CM C  10     1555   1555  1.60  
LINK         O3' 5CM C  10                 P    DA C  11     1555   1555  1.61  
LINK         O3'  DG D 107                 P   5CM D 108     1555   1555  1.61  
LINK         O3' 5CM D 108                 P    DG D 109     1555   1555  1.61  
LINK         O3'  DG D 109                 P   5CM D 110     1555   1555  1.61  
LINK         O3' 5CM D 110                 P    DA D 111     1555   1555  1.61  
SITE     1 AC1  4 ASN A 281   DA C  11   DA C  12  HOH C 230                    
SITE     1 AC2  5  DT C   1   DA C   2   DA C  14  HOH C 205                    
SITE     2 AC2  5  DA D 102                                                     
SITE     1 AC3  5  DA C   2   DT D 101   DA D 102   DA D 114                    
SITE     2 AC3  5 HOH D 503                                                     
SITE     1 AC4  5 ARG B 277  ASN B 281   DA D 111   DA D 112                    
SITE     2 AC4  5 HOH D 526                                                     
SITE     1 AC5  4  DT C  15   DA C  16   DT D 115   DA D 116                    
SITE     1 AC6 14 ARG A 289  ARG B 289   DT C   6   DG C   9                    
SITE     2 AC6 14 HOH C 218  HOH C 220  HOH C 224  HOH C 225                    
SITE     3 AC6 14 HOH C 226  HOH C 227  5CM D 108   DG D 109                    
SITE     4 AC6 14 5CM D 110   DA D 111                                          
SITE     1 AC7 14 ASN A 282  ARG A 286  ARG A 289  ARG B 289                    
SITE     2 AC7 14  DG C   7  5CM C  10  HOH C 216  HOH C 220                    
SITE     3 AC7 14 HOH C 221  HOH C 225  HOH C 227   DG D 107                    
SITE     4 AC7 14 5CM D 108   DG D 109                                          
SITE     1 AC8 14 ARG A 278  ASN A 282  ARG A 286  ARG A 289                    
SITE     2 AC8 14 5CM C   8   DA C  11  HOH C 208  HOH C 212                    
SITE     3 AC8 14 HOH C 216  HOH C 221   DT D 106   DG D 107                    
SITE     4 AC8 14 5CM D 108   DG D 109                                          
SITE     1 AC9 16 TYR A 274  ARG A 278  ASN A 281  ASN A 282                    
SITE     2 AC9 16 ARG A 289   DG C   9   DA C  12  EDO C 101                    
SITE     3 AC9 16 HOH C 208  HOH C 212  HOH C 216  HOH C 219                    
SITE     4 AC9 16 HOH C 223   DT D 105   DT D 106   DG D 107                    
SITE     1 AD1 14 ARG A 289  ARG B 289  LYS B 293  5CM C   8                    
SITE     2 AD1 14  DG C   9  5CM C  10   DA C  11   DT D 106                    
SITE     3 AD1 14  DG D 109  HOH D 514  HOH D 515  HOH D 527                    
SITE     4 AD1 14 HOH D 529  HOH D 530                                          
SITE     1 AD2 16 ARG A 289  ASN B 282  ARG B 286  ARG B 289                    
SITE     2 AD2 16  DG C   7  5CM C   8   DG C   9   DG D 107                    
SITE     3 AD2 16 5CM D 110  HOH D 509  HOH D 515  HOH D 520                    
SITE     4 AD2 16 HOH D 527  HOH D 528  HOH D 529  HOH D 531                    
SITE     1 AD3 16 ARG B 278  ASN B 282  ARG B 286  ARG B 289                    
SITE     2 AD3 16  DT C   6   DG C   7  5CM C   8   DG C   9                    
SITE     3 AD3 16 5CM D 108   DA D 111  HOH D 502  HOH D 509                    
SITE     4 AD3 16 HOH D 513  HOH D 520  HOH D 528  HOH D 531                    
SITE     1 AD4 15 TYR B 274  ARG B 278  ASN B 281  ASN B 282                    
SITE     2 AD4 15 ARG B 289   DT C   5   DT C   6   DG C   7                    
SITE     3 AD4 15  DG D 109   DA D 112  EDO D 402  HOH D 502                    
SITE     4 AD4 15 HOH D 513  HOH D 523  HOH D 524                               
CRYST1   48.110   77.405   99.825  90.00 103.90  90.00 C 1 2 1       8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.020786  0.000000  0.005145        0.00000                         
SCALE2      0.000000  0.012919  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.010320        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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