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Database: PDB
Entry: 6MG2
LinkDB: 6MG2
Original site: 6MG2 
HEADER    TRANSCRIPTION/DNA                       12-SEP-18   6MG2              
TITLE     C-TERMINAL BZIP DOMAIN OF HUMAN C/EBPBETA WITH 16BP METHYLATED        
TITLE    2 OLIGONUCLEOTIDE CONTAINING CONSENSUS RECOGNITION SEQUENCE-C2221      
TITLE    3 CRYSTAL FORM                                                         
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CCAAT/ENHANCER-BINDING PROTEIN BETA;                       
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: C/EBP BETA,LIVER ACTIVATOR PROTEIN,LAP,LIVER-ENRICHED       
COMPND   5 INHIBITORY PROTEIN,LIP,NUCLEAR FACTOR NF-IL6,TRANSCRIPTION FACTOR 5, 
COMPND   6 TCF-5;                                                               
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MOL_ID: 2;                                                           
COMPND   9 MOLECULE: 16-BP METHYLATED OLIGONUCLEOTIDE;                          
COMPND  10 CHAIN: C, D;                                                         
COMPND  11 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: CEBPB, TCF5, PP9092;                                           
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 SYNTHETIC: YES;                                                      
SOURCE  11 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  12 ORGANISM_TAXID: 9606                                                 
KEYWDS    BZIP TRANSCIPTION FACTOR DNA METHYLATION CPA METHYLATION PROTEIN-DNA  
KEYWDS   2 COMPLEX, TRANSCRIPTION, TRANSCRIPTION-DNA COMPLEX                    
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.R.HORTON,X.CHENG,J.YANG                                             
REVDAT   4   01-JAN-20 6MG2    1       REMARK                                   
REVDAT   3   28-AUG-19 6MG2    1       JRNL   REMARK                            
REVDAT   2   16-JAN-19 6MG2    1       JRNL                                     
REVDAT   1   12-DEC-18 6MG2    0                                                
JRNL        AUTH   J.YANG,J.R.HORTON,D.WANG,R.REN,J.LI,D.SUN,Y.HUANG,X.ZHANG,   
JRNL        AUTH 2 R.M.BLUMENTHAL,X.CHENG                                       
JRNL        TITL   STRUCTURAL BASIS FOR EFFECTS OF CPA MODIFICATIONS ON C/EBP   
JRNL        TITL 2 BETA BINDING OF DNA.                                         
JRNL        REF    NUCLEIC ACIDS RES.            V.  47  1774 2019              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   30566668                                                     
JRNL        DOI    10.1093/NAR/GKY1264                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.93 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.13_2998: ???)                              
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.93                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 33.90                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.4                           
REMARK   3   NUMBER OF REFLECTIONS             : 63591                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.177                           
REMARK   3   R VALUE            (WORKING SET) : 0.175                           
REMARK   3   FREE R VALUE                     : 0.210                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.970                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 3162                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 33.9086 -  5.4756    1.00     2645   137  0.1555 0.2098        
REMARK   3     2  5.4756 -  4.3491    1.00     2647   140  0.1588 0.1989        
REMARK   3     3  4.3491 -  3.8002    1.00     2626   137  0.1315 0.1636        
REMARK   3     4  3.8002 -  3.4531    1.00     2645   137  0.1548 0.1637        
REMARK   3     5  3.4531 -  3.2058    0.99     2635   133  0.1597 0.2192        
REMARK   3     6  3.2058 -  3.0169    0.99     2599   135  0.1877 0.2179        
REMARK   3     7  3.0169 -  2.8659    0.99     2658   140  0.1914 0.2396        
REMARK   3     8  2.8659 -  2.7412    1.00     2595   140  0.1913 0.2441        
REMARK   3     9  2.7412 -  2.6357    1.00     2661   138  0.1821 0.1909        
REMARK   3    10  2.6357 -  2.5448    1.00     2604   135  0.1776 0.2171        
REMARK   3    11  2.5448 -  2.4652    1.00     2669   142  0.1813 0.2034        
REMARK   3    12  2.4652 -  2.3948    1.00     2624   135  0.1902 0.2206        
REMARK   3    13  2.3948 -  2.3318    1.00     2662   141  0.1898 0.2015        
REMARK   3    14  2.3318 -  2.2749    1.00     2636   136  0.1912 0.1986        
REMARK   3    15  2.2749 -  2.2232    1.00     2637   138  0.1891 0.2506        
REMARK   3    16  2.2232 -  2.1759    1.00     2663   138  0.2022 0.2162        
REMARK   3    17  2.1759 -  2.1324    1.00     2643   139  0.2098 0.2698        
REMARK   3    18  2.1324 -  2.0921    1.00     2638   134  0.2204 0.2569        
REMARK   3    19  2.0921 -  2.0548    1.00     2634   142  0.2397 0.2859        
REMARK   3    20  2.0548 -  2.0199    1.00     2620   138  0.2440 0.2630        
REMARK   3    21  2.0199 -  1.9874    1.00     2641   136  0.2543 0.2478        
REMARK   3    22  1.9874 -  1.9568    0.99     2586   141  0.2709 0.3128        
REMARK   3    23  1.9568 -  1.9280    0.92     2461   130  0.3036 0.3470        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.210            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 20.460           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.005           1869                                  
REMARK   3   ANGLE     :  0.939           2647                                  
REMARK   3   CHIRALITY :  0.041            293                                  
REMARK   3   PLANARITY :  0.004            231                                  
REMARK   3   DIHEDRAL  : 25.057           1031                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 4                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 268 THROUGH 332 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  27.3083  52.4401  34.0304              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3874 T22:   0.3392                                     
REMARK   3      T33:   0.2908 T12:   0.0468                                     
REMARK   3      T13:  -0.0271 T23:   0.0208                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.5008 L22:   6.8421                                     
REMARK   3      L33:   2.7615 L12:  -3.4215                                     
REMARK   3      L13:   2.0984 L23:  -5.2150                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0805 S12:  -0.2352 S13:  -0.1215                       
REMARK   3      S21:  -0.0338 S22:   0.3926 S23:   0.3179                       
REMARK   3      S31:   0.0301 S32:  -0.2358 S33:  -0.3137                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 268 THROUGH 336 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  24.7420  53.4369  25.0202              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2106 T22:   0.2379                                     
REMARK   3      T33:   0.1727 T12:   0.0087                                     
REMARK   3      T13:   0.0093 T23:  -0.0155                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.6457 L22:   2.9818                                     
REMARK   3      L33:   0.7851 L12:   1.1897                                     
REMARK   3      L13:  -0.1866 L23:  -1.1252                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0232 S12:   0.0236 S13:  -0.0512                       
REMARK   3      S21:  -0.2530 S22:  -0.0378 S23:  -0.0531                       
REMARK   3      S31:   0.0509 S32:  -0.0253 S33:   0.0505                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 1 THROUGH 16 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):  28.4267  28.0647  36.2443              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2191 T22:   0.2653                                     
REMARK   3      T33:   0.1742 T12:   0.0264                                     
REMARK   3      T13:   0.0305 T23:   0.0083                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.3443 L22:   1.6076                                     
REMARK   3      L33:   2.2771 L12:   0.3594                                     
REMARK   3      L13:   3.4186 L23:   0.6880                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1040 S12:   0.2795 S13:  -0.1788                       
REMARK   3      S21:  -0.1024 S22:   0.0025 S23:  -0.0458                       
REMARK   3      S31:   0.0910 S32:   0.1680 S33:   0.0919                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 101 THROUGH 116 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  27.6257  28.7966  38.1146              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2382 T22:   0.2587                                     
REMARK   3      T33:   0.2283 T12:   0.0242                                     
REMARK   3      T13:   0.0305 T23:  -0.0014                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.4730 L22:   0.9898                                     
REMARK   3      L33:   2.6475 L12:   1.2987                                     
REMARK   3      L13:   2.1342 L23:   0.3035                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0421 S12:  -0.2594 S13:   0.0770                       
REMARK   3      S21:  -0.0124 S22:  -0.0148 S23:  -0.0217                       
REMARK   3      S31:   0.1397 S32:   0.0540 S33:   0.0585                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 6MG2 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 13-SEP-18.                  
REMARK 100 THE DEPOSITION ID IS D_1000236843.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 03-FEB-18                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 7.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : N                                  
REMARK 200  RADIATION SOURCE               : SEALED TUBE                        
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU MICROMAX-003                
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.00000                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU HYPIX-6000HE                
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 63673                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.928                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 33.903                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8                               
REMARK 200  DATA REDUNDANCY                : 16.60                              
REMARK 200  R MERGE                    (I) : 0.08100                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 31.2000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.93                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.00                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.6                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 12.80                              
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.700                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHENIX                                                
REMARK 200 STARTING MODEL: 2E42                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 67.98                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.84                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M SODIUM ACETATE TRIHYDRATE PH       
REMARK 280  7.0, 12% (W/V) POLYETHYLENE GLYCOL 3350, VAPOR DIFFUSION,           
REMARK 280  SITTING DROP, TEMPERATURE 292K                                      
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -X,Y,-Z+1/2                                             
REMARK 290       4555   X,-Y,-Z                                                 
REMARK 290       5555   X+1/2,Y+1/2,Z                                           
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2                                     
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2                                     
REMARK 290       8555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       37.82650            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       37.82650            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       50.92700            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       56.82400            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       50.92700            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       56.82400            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       37.82650            
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       50.92700            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       56.82400            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       37.82650            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       50.92700            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       56.82400            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 9480 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 13490 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -3.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     HIS A   267                                                      
REMARK 465     GLN A   333                                                      
REMARK 465     LEU A   334                                                      
REMARK 465     PRO A   335                                                      
REMARK 465     GLU A   336                                                      
REMARK 465     PRO A   337                                                      
REMARK 465     LEU A   338                                                      
REMARK 465     LEU A   339                                                      
REMARK 465     ALA A   340                                                      
REMARK 465     SER A   341                                                      
REMARK 465     SER A   342                                                      
REMARK 465     GLY A   343                                                      
REMARK 465     HIS A   344                                                      
REMARK 465     HIS B   267                                                      
REMARK 465     PRO B   337                                                      
REMARK 465     LEU B   338                                                      
REMARK 465     LEU B   339                                                      
REMARK 465     ALA B   340                                                      
REMARK 465     SER B   341                                                      
REMARK 465     SER B   342                                                      
REMARK 465     GLY B   343                                                      
REMARK 465     HIS B   344                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     MET A 268    CG   SD   CE                                        
REMARK 470     GLU A 273    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 277    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS A 332    CG   CD   CE   NZ                                   
REMARK 470     MET B 268    CG   SD   CE                                        
REMARK 470     LYS B 269    CG   CD   CE   NZ                                   
REMARK 470     HIS B 270    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLU B 273    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 275    CG   CD   CE   NZ                                   
REMARK 470     GLU B 279    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 291    CG   CD   CE   NZ                                   
REMARK 470     GLU B 336    CG   CD   OE1  OE2                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    HIS B 270       61.63   -112.77                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO A 401                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO D 501                 
DBREF  6MG2 A  269   344  UNP    P17676   CEBPB_HUMAN    246    321             
DBREF  6MG2 B  269   344  UNP    P17676   CEBPB_HUMAN    246    321             
DBREF  6MG2 C    1    16  PDB    6MG2     6MG2             1     16             
DBREF  6MG2 D  101   116  PDB    6MG2     6MG2           101    116             
SEQADV 6MG2 HIS A  267  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG2 MET A  268  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG2 HIS B  267  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG2 MET B  268  UNP  P17676              EXPRESSION TAG                 
SEQRES   1 A   78  HIS MET LYS HIS SER ASP GLU TYR LYS ILE ARG ARG GLU          
SEQRES   2 A   78  ARG ASN ASN ILE ALA VAL ARG LYS SER ARG ASP LYS ALA          
SEQRES   3 A   78  LYS MET ARG ASN LEU GLU THR GLN HIS LYS VAL LEU GLU          
SEQRES   4 A   78  LEU THR ALA GLU ASN GLU ARG LEU GLN LYS LYS VAL GLU          
SEQRES   5 A   78  GLN LEU SER ARG GLU LEU SER THR LEU ARG ASN LEU PHE          
SEQRES   6 A   78  LYS GLN LEU PRO GLU PRO LEU LEU ALA SER SER GLY HIS          
SEQRES   1 B   78  HIS MET LYS HIS SER ASP GLU TYR LYS ILE ARG ARG GLU          
SEQRES   2 B   78  ARG ASN ASN ILE ALA VAL ARG LYS SER ARG ASP LYS ALA          
SEQRES   3 B   78  LYS MET ARG ASN LEU GLU THR GLN HIS LYS VAL LEU GLU          
SEQRES   4 B   78  LEU THR ALA GLU ASN GLU ARG LEU GLN LYS LYS VAL GLU          
SEQRES   5 B   78  GLN LEU SER ARG GLU LEU SER THR LEU ARG ASN LEU PHE          
SEQRES   6 B   78  LYS GLN LEU PRO GLU PRO LEU LEU ALA SER SER GLY HIS          
SEQRES   1 C   16   DT  DA  DT  DA  DT  DT  DG 5CM  DG 5CM  DA  DA  DT          
SEQRES   2 C   16   DA  DT  DA                                                  
SEQRES   1 D   16   DT  DA  DT  DA  DT  DT  DG 5CM  DG 5CM  DA  DA  DT          
SEQRES   2 D   16   DA  DT  DA                                                  
HET    5CM  C   8      20                                                       
HET    5CM  C  10      20                                                       
HET    5CM  D 108      20                                                       
HET    5CM  D 110      20                                                       
HET    EDO  A 401       4                                                       
HET    EDO  D 501       4                                                       
HETNAM     5CM 5-METHYL-2'-DEOXY-CYTIDINE-5'-MONOPHOSPHATE                      
HETNAM     EDO 1,2-ETHANEDIOL                                                   
HETSYN     EDO ETHYLENE GLYCOL                                                  
FORMUL   3  5CM    4(C10 H16 N3 O7 P)                                           
FORMUL   5  EDO    2(C2 H6 O2)                                                  
FORMUL   7  HOH   *290(H2 O)                                                    
HELIX    1 AA1 SER A  271  ASN A  329  1                                  59    
HELIX    2 AA2 SER B  271  GLN B  333  1                                  63    
LINK         O3'  DG C   7                 P   5CM C   8     1555   1555  1.60  
LINK         O3' 5CM C   8                 P    DG C   9     1555   1555  1.61  
LINK         O3'  DG C   9                 P   5CM C  10     1555   1555  1.61  
LINK         O3' 5CM C  10                 P    DA C  11     1555   1555  1.61  
LINK         O3'  DG D 107                 P   5CM D 108     1555   1555  1.60  
LINK         O3' 5CM D 108                 P    DG D 109     1555   1555  1.61  
LINK         O3'  DG D 109                 P   5CM D 110     1555   1555  1.61  
LINK         O3' 5CM D 110                 P    DA D 111     1555   1555  1.61  
CISPEP   1 LYS B  269    HIS B  270          0        -8.21                     
SITE     1 AC1  4 ASP A 290  LYS A 293   DG C   7  HOH C 121                    
SITE     1 AC2  3 HOH B 405   DA D 111   DA D 112                               
CRYST1  101.854  113.648   75.653  90.00  90.00  90.00 C 2 2 21     16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009818  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.008799  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.013218        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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