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Database: PDB
Entry: 6MG3
LinkDB: 6MG3
Original site: 6MG3 
HEADER    TRANSCRIPTION/DNA                       12-SEP-18   6MG3              
TITLE     V285A MUTANT OF THE C-TERMINAL BZIP DOMAIN OF HUMAN C/EBPBETA WITH    
TITLE    2 16BP METHYLATED OLIGONUCLEOTIDE CONTAINING CONSENSUS RECOGNITION     
TITLE    3 SEQUENCE                                                             
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CCAAT/ENHANCER-BINDING PROTEIN BETA;                       
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: C/EBP BETA,LIVER ACTIVATOR PROTEIN,LAP,LIVER-ENRICHED       
COMPND   5 INHIBITORY PROTEIN,LIP,NUCLEAR FACTOR NF-IL6,TRANSCRIPTION FACTOR 5, 
COMPND   6 TCF-5;                                                               
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MUTATION: YES;                                                       
COMPND   9 MOL_ID: 2;                                                           
COMPND  10 MOLECULE: 16-BP METHYLATED OLIGONUCLEOTIDE;                          
COMPND  11 CHAIN: C, D;                                                         
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: CEBPB, TCF5, PP9092;                                           
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 MOL_ID: 2;                                                           
SOURCE  10 SYNTHETIC: YES;                                                      
SOURCE  11 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  12 ORGANISM_TAXID: 9606                                                 
KEYWDS    BZIP TRANSCIPTION FACTOR DNA METHYLATION CPA METHYLATION PROTEIN-DNA  
KEYWDS   2 COMPLEX, TRANSCRIPTION, TRANSCRIPTION-DNA COMPLEX                    
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.R.HORTON,X.CHENG,J.YANG                                             
REVDAT   6   01-JAN-20 6MG3    1       REMARK                                   
REVDAT   5   28-AUG-19 6MG3    1       JRNL   REMARK                            
REVDAT   4   30-JAN-19 6MG3    1       REMARK                                   
REVDAT   3   16-JAN-19 6MG3    1       JRNL                                     
REVDAT   2   19-DEC-18 6MG3    1       REMARK                                   
REVDAT   1   12-DEC-18 6MG3    0                                                
JRNL        AUTH   J.YANG,J.R.HORTON,D.WANG,R.REN,J.LI,D.SUN,Y.HUANG,X.ZHANG,   
JRNL        AUTH 2 R.M.BLUMENTHAL,X.CHENG                                       
JRNL        TITL   STRUCTURAL BASIS FOR EFFECTS OF CPA MODIFICATIONS ON C/EBP   
JRNL        TITL 2 BETA BINDING OF DNA.                                         
JRNL        REF    NUCLEIC ACIDS RES.            V.  47  1774 2019              
JRNL        REFN                   ESSN 1362-4962                               
JRNL        PMID   30566668                                                     
JRNL        DOI    10.1093/NAR/GKY1264                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.05 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (1.13_2998: ???)                              
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : NULL                                          
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.05                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 26.73                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.340                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 27768                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.223                           
REMARK   3   R VALUE            (WORKING SET) : 0.222                           
REMARK   3   FREE R VALUE                     : 0.249                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.460                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1238                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 26.7366 -  4.2591    1.00     3083   145  0.1781 0.1975        
REMARK   3     2  4.2591 -  3.3827    1.00     2981   139  0.1938 0.2371        
REMARK   3     3  3.3827 -  2.9557    0.99     2949   138  0.2312 0.2692        
REMARK   3     4  2.9557 -  2.6858    1.00     2926   136  0.2422 0.2417        
REMARK   3     5  2.6858 -  2.4934    1.00     2931   137  0.2371 0.3082        
REMARK   3     6  2.4934 -  2.3465    1.00     2932   137  0.2472 0.2468        
REMARK   3     7  2.3465 -  2.2291    1.00     2901   136  0.2727 0.2865        
REMARK   3     8  2.2291 -  2.1321    1.00     2913   136  0.2945 0.3281        
REMARK   3     9  2.1321 -  2.0500    1.00     2914   134  0.3205 0.3141        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : NULL                                          
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.260            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 25.610           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.003           1947                                  
REMARK   3   ANGLE     :  0.651           2769                                  
REMARK   3   CHIRALITY :  0.027            301                                  
REMARK   3   PLANARITY :  0.002            236                                  
REMARK   3   DIHEDRAL  : 24.408           1064                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 4                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 269 THROUGH 332 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  27.0403  53.7085  33.4318              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2261 T22:   0.2558                                     
REMARK   3      T33:   0.2077 T12:   0.0248                                     
REMARK   3      T13:   0.0253 T23:  -0.0045                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.7031 L22:   8.3859                                     
REMARK   3      L33:   2.9286 L12:  -1.6578                                     
REMARK   3      L13:   1.0878 L23:  -4.8797                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1697 S12:  -0.1995 S13:  -0.0789                       
REMARK   3      S21:   0.3481 S22:   0.5235 S23:   0.4591                       
REMARK   3      S31:  -0.1436 S32:  -0.2491 S33:  -0.4092                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 269 THROUGH 336 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  24.6496  53.2534  25.0134              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2509 T22:   0.2617                                     
REMARK   3      T33:   0.2101 T12:   0.0127                                     
REMARK   3      T13:   0.0151 T23:  -0.0124                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.5324 L22:   7.9160                                     
REMARK   3      L33:   0.5736 L12:   1.2141                                     
REMARK   3      L13:   0.0104 L23:  -0.5358                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1075 S12:   0.0179 S13:  -0.0266                       
REMARK   3      S21:   0.1968 S22:  -0.1536 S23:  -0.0089                       
REMARK   3      S31:  -0.0295 S32:  -0.0344 S33:   0.0837                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 1 THROUGH 16 )                    
REMARK   3    ORIGIN FOR THE GROUP (A):  28.5374  28.3994  35.9437              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2300 T22:   0.3563                                     
REMARK   3      T33:   0.2459 T12:   0.0531                                     
REMARK   3      T13:   0.0291 T23:  -0.0519                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.0606 L22:   2.4663                                     
REMARK   3      L33:   2.2588 L12:   0.7488                                     
REMARK   3      L13:   1.1178 L23:   0.0487                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1499 S12:   0.4582 S13:  -0.3916                       
REMARK   3      S21:  -0.0287 S22:   0.1246 S23:  -0.1066                       
REMARK   3      S31:   0.2016 S32:   0.4646 S33:   0.0449                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 101 THROUGH 116 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  27.8923  28.6667  36.9895              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2832 T22:   0.3569                                     
REMARK   3      T33:   0.2109 T12:  -0.0002                                     
REMARK   3      T13:   0.0492 T23:  -0.0061                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   7.9185 L22:   1.5607                                     
REMARK   3      L33:   3.4885 L12:   2.6694                                     
REMARK   3      L13:   3.3950 L23:   0.7794                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.3038 S12:   0.4195 S13:  -0.0551                       
REMARK   3      S21:  -0.1076 S22:   0.2564 S23:  -0.1471                       
REMARK   3      S31:   0.2634 S32:   0.3600 S33:   0.0618                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 6MG3 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 13-SEP-18.                  
REMARK 100 THE DEPOSITION ID IS D_1000236853.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 26-JUL-18                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 7.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : N                                  
REMARK 200  RADIATION SOURCE               : SEALED TUBE                        
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU MICROMAX-003                
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.54218                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU HYPIX-6000HE                
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : CRYSALISPRO                        
REMARK 200  DATA SCALING SOFTWARE          : CRYSALISPRO                        
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 28807                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.050                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 26.734                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY                : 8.200                              
REMARK 200  R MERGE                    (I) : 0.17200                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 9.5000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.05                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.12                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.70                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.000                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHENIX                                                
REMARK 200 STARTING MODEL: 2E42                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 67.93                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.84                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1 M SODIUM ACETATE TRIHYDRATE PH       
REMARK 280  7.0, 12% (W/V) POLYETHYLENE GLYCOL 3350, VAPOR DIFFUSION,           
REMARK 280  SITTING DROP, TEMPERATURE 292K                                      
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -X,Y,-Z+1/2                                             
REMARK 290       4555   X,-Y,-Z                                                 
REMARK 290       5555   X+1/2,Y+1/2,Z                                           
REMARK 290       6555   -X+1/2,-Y+1/2,Z+1/2                                     
REMARK 290       7555   -X+1/2,Y+1/2,-Z+1/2                                     
REMARK 290       8555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       37.72200            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       37.72200            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       50.71500            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       57.02100            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       50.71500            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       57.02100            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       37.72200            
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       50.71500            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       57.02100            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       37.72200            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       50.71500            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       57.02100            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 9320 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 13550 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -9.0 KCAL/MOL                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     HIS A   267                                                      
REMARK 465     MET A   268                                                      
REMARK 465     GLN A   333                                                      
REMARK 465     LEU A   334                                                      
REMARK 465     PRO A   335                                                      
REMARK 465     GLU A   336                                                      
REMARK 465     PRO A   337                                                      
REMARK 465     LEU A   338                                                      
REMARK 465     LEU A   339                                                      
REMARK 465     ALA A   340                                                      
REMARK 465     SER A   341                                                      
REMARK 465     SER A   342                                                      
REMARK 465     GLY A   343                                                      
REMARK 465     HIS A   344                                                      
REMARK 465     HIS B   267                                                      
REMARK 465     MET B   268                                                      
REMARK 465     PRO B   337                                                      
REMARK 465     LEU B   338                                                      
REMARK 465     LEU B   339                                                      
REMARK 465     ALA B   340                                                      
REMARK 465     SER B   341                                                      
REMARK 465     SER B   342                                                      
REMARK 465     GLY B   343                                                      
REMARK 465     HIS B   344                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LYS A 269    CG   CD   CE   NZ                                   
REMARK 470     GLU A 273    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 275    CG   CD   CE   NZ                                   
REMARK 470     ARG A 277    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS B 269    CG   CD   CE   NZ                                   
REMARK 470     GLU B 273    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 275    CG   CD   CE   NZ                                   
REMARK 470     GLU B 336    CG   CD   OE1  OE2                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    SER B 271     -161.10   -108.29                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525                                                                      
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT                    
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST                  
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT                 
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE                       
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;                             
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE                  
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH B 474        DISTANCE =  9.19 ANGSTROMS                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: binding site for residue EDO C 401                 
DBREF  6MG3 A  269   344  UNP    P17676   CEBPB_HUMAN    246    321             
DBREF  6MG3 B  269   344  UNP    P17676   CEBPB_HUMAN    246    321             
DBREF  6MG3 C    1    16  PDB    6MG3     6MG3             1     16             
DBREF  6MG3 D  101   116  PDB    6MG3     6MG3           101    116             
SEQADV 6MG3 HIS A  267  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG3 MET A  268  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG3 ALA A  285  UNP  P17676    VAL   262 ENGINEERED MUTATION            
SEQADV 6MG3 HIS B  267  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG3 MET B  268  UNP  P17676              EXPRESSION TAG                 
SEQADV 6MG3 ALA B  285  UNP  P17676    VAL   262 ENGINEERED MUTATION            
SEQRES   1 A   78  HIS MET LYS HIS SER ASP GLU TYR LYS ILE ARG ARG GLU          
SEQRES   2 A   78  ARG ASN ASN ILE ALA ALA ARG LYS SER ARG ASP LYS ALA          
SEQRES   3 A   78  LYS MET ARG ASN LEU GLU THR GLN HIS LYS VAL LEU GLU          
SEQRES   4 A   78  LEU THR ALA GLU ASN GLU ARG LEU GLN LYS LYS VAL GLU          
SEQRES   5 A   78  GLN LEU SER ARG GLU LEU SER THR LEU ARG ASN LEU PHE          
SEQRES   6 A   78  LYS GLN LEU PRO GLU PRO LEU LEU ALA SER SER GLY HIS          
SEQRES   1 B   78  HIS MET LYS HIS SER ASP GLU TYR LYS ILE ARG ARG GLU          
SEQRES   2 B   78  ARG ASN ASN ILE ALA ALA ARG LYS SER ARG ASP LYS ALA          
SEQRES   3 B   78  LYS MET ARG ASN LEU GLU THR GLN HIS LYS VAL LEU GLU          
SEQRES   4 B   78  LEU THR ALA GLU ASN GLU ARG LEU GLN LYS LYS VAL GLU          
SEQRES   5 B   78  GLN LEU SER ARG GLU LEU SER THR LEU ARG ASN LEU PHE          
SEQRES   6 B   78  LYS GLN LEU PRO GLU PRO LEU LEU ALA SER SER GLY HIS          
SEQRES   1 C   16   DT  DA  DT  DA  DT  DT  DG 5CM  DG 5CM  DA  DA  DT          
SEQRES   2 C   16   DA  DT  DA                                                  
SEQRES   1 D   16   DT  DA  DT  DA  DT  DT  DG 5CM  DG 5CM  DA  DA  DT          
SEQRES   2 D   16   DA  DT  DA                                                  
HET    5CM  C   8      20                                                       
HET    5CM  C  10      20                                                       
HET    5CM  D 108      40                                                       
HET    5CM  D 110      20                                                       
HET    EDO  C 401       4                                                       
HETNAM     5CM 5-METHYL-2'-DEOXY-CYTIDINE-5'-MONOPHOSPHATE                      
HETNAM     EDO 1,2-ETHANEDIOL                                                   
HETSYN     EDO ETHYLENE GLYCOL                                                  
FORMUL   3  5CM    4(C10 H16 N3 O7 P)                                           
FORMUL   5  EDO    C2 H6 O2                                                     
FORMUL   6  HOH   *250(H2 O)                                                    
HELIX    1 AA1 SER A  271  ASN A  329  1                                  59    
HELIX    2 AA2 SER B  271  GLN B  333  1                                  63    
LINK         O3'  DG C   7                 P   5CM C   8     1555   1555  1.61  
LINK         O3' 5CM C   8                 P    DG C   9     1555   1555  1.61  
LINK         O3'  DG C   9                 P   5CM C  10     1555   1555  1.61  
LINK         O3' 5CM C  10                 P    DA C  11     1555   1555  1.61  
LINK         O3'A DG D 107                 P  A5CM D 108     1555   1555  1.61  
LINK         O3'B DG D 107                 P  B5CM D 108     1555   1555  1.61  
LINK         O3'A5CM D 108                 P  A DG D 109     1555   1555  1.61  
LINK         O3'B5CM D 108                 P  B DG D 109     1555   1555  1.61  
LINK         O3'A DG D 109                 P   5CM D 110     1555   1555  1.61  
LINK         O3'B DG D 109                 P   5CM D 110     1555   1555  1.61  
LINK         O3' 5CM D 110                 P    DA D 111     1555   1555  1.61  
SITE     1 AC1  4  DG C   9  5CM C  10   DG D 109  5CM D 110                    
CRYST1  101.430  114.042   75.444  90.00  90.00  90.00 C 2 2 21     16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.009859  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.008769  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.013255        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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