GenomeNet

Database: PDB
Entry: 6MG8
LinkDB: 6MG8
Original site: 6MG8 
HEADER    MEMBRANE PROTEIN                        13-SEP-18   6MG8              
TITLE     STRUCTURAL BASIS FOR CHOLESTEROL TRANSPORT-LIKE ACTIVITY OF THE       
TITLE    2 HEDGEHOG RECEPTOR PATCHED                                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN PATCHED HOMOLOG 1;                                 
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: PTC1;                                                       
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: MOUSE;                                              
SOURCE   4 ORGANISM_TAXID: 10090;                                               
SOURCE   5 GENE: PTCH1, PTCH;                                                   
SOURCE   6 EXPRESSION_SYSTEM: HOMO SAPIENS;                                     
SOURCE   7 EXPRESSION_SYSTEM_COMMON: HUMAN;                                     
SOURCE   8 EXPRESSION_SYSTEM_TAXID: 9606;                                       
SOURCE   9 EXPRESSION_SYSTEM_CELL_LINE: HEK293;                                 
SOURCE  10 EXPRESSION_SYSTEM_VECTOR: BACMAM                                     
KEYWDS    RECEPTOR MEMBRANE PROTEIN, MEMBRANE PROTEIN                           
EXPDTA    ELECTRON MICROSCOPY                                                   
AUTHOR    Y.ZHANG,D.BULKLEY,Y.XIN,K.J.ROBERTS,D.E.ASARNOW,A.SHARMA,B.R.MYERS,   
AUTHOR   2 W.CHO,Y.CHENG,P.A.BEACHY                                             
REVDAT   1   28-NOV-18 6MG8    0                                                
JRNL        AUTH   Y.ZHANG,D.P.BULKLEY,Y.XIN,K.J.ROBERTS,D.E.ASARNOW,A.SHARMA,  
JRNL        AUTH 2 B.R.MYERS,W.CHO,Y.CHENG,P.A.BEACHY                           
JRNL        TITL   STRUCTURAL BASIS FOR CHOLESTEROL TRANSPORT-LIKE ACTIVITY OF  
JRNL        TITL 2 THE HEDGEHOG RECEPTOR PATCHED.                               
JRNL        REF    CELL                          V. 175  1352 2018              
JRNL        REFN                   ISSN 1097-4172                               
JRNL        PMID   30415841                                                     
JRNL        DOI    10.1016/J.CELL.2018.10.026                                   
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.60 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : SERIALEM, GCTF, COOT, CRYOSPARC,          
REMARK   3                            RELION, RELION, RELION, PHENIX            
REMARK   3   RECONSTRUCTION SCHEMA  : NULL                                      
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : NULL                                
REMARK   3   REFINEMENT SPACE             : REAL                                
REMARK   3   REFINEMENT PROTOCOL          : AB INITIO MODEL                     
REMARK   3   REFINEMENT TARGET            : NULL                                
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : NULL                                             
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : NULL                           
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : NULL                           
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 3.600                          
REMARK   3   NUMBER OF PARTICLES               : 245725                         
REMARK   3   CTF CORRECTION METHOD             : PHASE FLIPPING AND AMPLITUDE   
REMARK   3                                       CORRECTION                     
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL                    
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: NULL                                                  
REMARK   4                                                                      
REMARK   4 6MG8 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 13-SEP-18.                  
REMARK 100 THE DEPOSITION ID IS D_1000236214.                                   
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : SINGLE PARTICLE                   
REMARK 245   SPECIMEN TYPE                  : NULL                              
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : PARTICLE                          
REMARK 245   PARTICLE TYPE                  : POINT                             
REMARK 245   NAME OF SAMPLE                 : PATCHED1 PROTEIN SOLUBILIZED IN   
REMARK 245                                    AMPHIPOL                          
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : 0.30                              
REMARK 245   SAMPLE SUPPORT DETAILS         : NULL                              
REMARK 245   SAMPLE VITRIFICATION DETAILS   : NULL                              
REMARK 245   SAMPLE BUFFER                  : NULL                              
REMARK 245   PH                             : 7.40                              
REMARK 245   SAMPLE DETAILS                 : NULL                              
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : NULL                           
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : 5236                           
REMARK 245   TEMPERATURE (KELVIN)              : NULL                           
REMARK 245   MICROSCOPE MODEL                  : FEI TITAN KRIOS                
REMARK 245   DETECTOR TYPE                     : GATAN K2 SUMMIT (4K X 4K)      
REMARK 245   MINIMUM DEFOCUS (NM)              : NULL                           
REMARK 245   MAXIMUM DEFOCUS (NM)              : NULL                           
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   NOMINAL CS                        : 2.70                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 38.00                          
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : NULL                           
REMARK 245   CALIBRATED MAGNIFICATION          : NULL                           
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 300                            
REMARK 245   IMAGING DETAILS                   : NULL                           
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     ALA A     2                                                      
REMARK 465     SER A     3                                                      
REMARK 465     ALA A     4                                                      
REMARK 465     GLY A     5                                                      
REMARK 465     ASN A     6                                                      
REMARK 465     ALA A     7                                                      
REMARK 465     ALA A     8                                                      
REMARK 465     GLY A     9                                                      
REMARK 465     ALA A    10                                                      
REMARK 465     LEU A    11                                                      
REMARK 465     GLY A    12                                                      
REMARK 465     ARG A    13                                                      
REMARK 465     GLN A    14                                                      
REMARK 465     ALA A    15                                                      
REMARK 465     GLY A    16                                                      
REMARK 465     GLY A    17                                                      
REMARK 465     GLY A    18                                                      
REMARK 465     ARG A    19                                                      
REMARK 465     ARG A    20                                                      
REMARK 465     ARG A    21                                                      
REMARK 465     ARG A    22                                                      
REMARK 465     THR A    23                                                      
REMARK 465     GLY A    24                                                      
REMARK 465     GLY A    25                                                      
REMARK 465     PRO A    26                                                      
REMARK 465     HIS A    27                                                      
REMARK 465     ARG A    28                                                      
REMARK 465     ALA A    29                                                      
REMARK 465     ALA A    30                                                      
REMARK 465     PRO A    31                                                      
REMARK 465     ASP A    32                                                      
REMARK 465     ARG A    33                                                      
REMARK 465     ASP A    34                                                      
REMARK 465     TYR A    35                                                      
REMARK 465     LEU A    36                                                      
REMARK 465     HIS A    37                                                      
REMARK 465     ARG A    38                                                      
REMARK 465     PRO A    39                                                      
REMARK 465     SER A    40                                                      
REMARK 465     TYR A    41                                                      
REMARK 465     CYS A    42                                                      
REMARK 465     ASP A    43                                                      
REMARK 465     ALA A    44                                                      
REMARK 465     ALA A    45                                                      
REMARK 465     PHE A    46                                                      
REMARK 465     ALA A    47                                                      
REMARK 465     LEU A    48                                                      
REMARK 465     GLU A    49                                                      
REMARK 465     GLN A    50                                                      
REMARK 465     ILE A    51                                                      
REMARK 465     SER A    52                                                      
REMARK 465     LYS A    53                                                      
REMARK 465     GLY A    54                                                      
REMARK 465     LYS A    55                                                      
REMARK 465     ALA A    56                                                      
REMARK 465     THR A    57                                                      
REMARK 465     ASP A   615                                                      
REMARK 465     ARG A   616                                                      
REMARK 465     ARG A   617                                                      
REMARK 465     LEU A   618                                                      
REMARK 465     ASP A   619                                                      
REMARK 465     ILE A   620                                                      
REMARK 465     PHE A   621                                                      
REMARK 465     CYS A   622                                                      
REMARK 465     CYS A   623                                                      
REMARK 465     PHE A   624                                                      
REMARK 465     THR A   625                                                      
REMARK 465     SER A   626                                                      
REMARK 465     PRO A   627                                                      
REMARK 465     CYS A   628                                                      
REMARK 465     VAL A   629                                                      
REMARK 465     SER A   630                                                      
REMARK 465     ARG A   631                                                      
REMARK 465     VAL A   632                                                      
REMARK 465     ILE A   633                                                      
REMARK 465     GLN A   634                                                      
REMARK 465     VAL A   635                                                      
REMARK 465     GLU A   636                                                      
REMARK 465     PRO A   637                                                      
REMARK 465     GLN A   638                                                      
REMARK 465     ALA A   639                                                      
REMARK 465     TYR A   640                                                      
REMARK 465     THR A   641                                                      
REMARK 465     GLU A   642                                                      
REMARK 465     PRO A   643                                                      
REMARK 465     MET A   644                                                      
REMARK 465     GLN A   645                                                      
REMARK 465     SER A   646                                                      
REMARK 465     THR A   647                                                      
REMARK 465     VAL A   648                                                      
REMARK 465     GLN A   649                                                      
REMARK 465     LEU A   650                                                      
REMARK 465     ARG A   651                                                      
REMARK 465     THR A   652                                                      
REMARK 465     GLU A   653                                                      
REMARK 465     TYR A   654                                                      
REMARK 465     ASP A   655                                                      
REMARK 465     PRO A   656                                                      
REMARK 465     HIS A   657                                                      
REMARK 465     THR A   658                                                      
REMARK 465     HIS A   659                                                      
REMARK 465     VAL A   660                                                      
REMARK 465     TYR A   661                                                      
REMARK 465     TYR A   662                                                      
REMARK 465     THR A   663                                                      
REMARK 465     THR A   664                                                      
REMARK 465     ALA A   665                                                      
REMARK 465     GLU A   666                                                      
REMARK 465     PRO A   667                                                      
REMARK 465     ARG A   668                                                      
REMARK 465     SER A   669                                                      
REMARK 465     GLU A   670                                                      
REMARK 465     ILE A   671                                                      
REMARK 465     SER A   672                                                      
REMARK 465     VAL A   673                                                      
REMARK 465     GLN A   674                                                      
REMARK 465     PRO A   675                                                      
REMARK 465     VAL A   676                                                      
REMARK 465     THR A   677                                                      
REMARK 465     VAL A   678                                                      
REMARK 465     THR A   679                                                      
REMARK 465     GLN A   680                                                      
REMARK 465     ASP A   681                                                      
REMARK 465     ASN A   682                                                      
REMARK 465     LEU A   683                                                      
REMARK 465     SER A   684                                                      
REMARK 465     CYS A   685                                                      
REMARK 465     GLN A   686                                                      
REMARK 465     SER A   687                                                      
REMARK 465     PRO A   688                                                      
REMARK 465     GLU A   689                                                      
REMARK 465     SER A   690                                                      
REMARK 465     THR A   691                                                      
REMARK 465     SER A   692                                                      
REMARK 465     SER A   693                                                      
REMARK 465     THR A   694                                                      
REMARK 465     ARG A   695                                                      
REMARK 465     ASP A   696                                                      
REMARK 465     LEU A   697                                                      
REMARK 465     LEU A   698                                                      
REMARK 465     SER A   699                                                      
REMARK 465     GLN A   700                                                      
REMARK 465     PHE A   701                                                      
REMARK 465     SER A   702                                                      
REMARK 465     ASP A   703                                                      
REMARK 465     SER A   704                                                      
REMARK 465     SER A   705                                                      
REMARK 465     LEU A   706                                                      
REMARK 465     HIS A   707                                                      
REMARK 465     CYS A   708                                                      
REMARK 465     LEU A   709                                                      
REMARK 465     GLU A   710                                                      
REMARK 465     PRO A   711                                                      
REMARK 465     PRO A   712                                                      
REMARK 465     CYS A   713                                                      
REMARK 465     THR A   714                                                      
REMARK 465     LYS A   715                                                      
REMARK 465     PRO A  1168                                                      
REMARK 465     GLU A  1169                                                      
REMARK 465     VAL A  1170                                                      
REMARK 465     SER A  1171                                                      
REMARK 465     PRO A  1172                                                      
REMARK 465     ALA A  1173                                                      
REMARK 465     ASN A  1174                                                      
REMARK 465     GLY A  1175                                                      
REMARK 465     LEU A  1176                                                      
REMARK 465     ASN A  1177                                                      
REMARK 465     ARG A  1178                                                      
REMARK 465     LEU A  1179                                                      
REMARK 465     PRO A  1180                                                      
REMARK 465     THR A  1181                                                      
REMARK 465     PRO A  1182                                                      
REMARK 465     SER A  1183                                                      
REMARK 465     PRO A  1184                                                      
REMARK 465     GLU A  1185                                                      
REMARK 465     PRO A  1186                                                      
REMARK 465     PRO A  1187                                                      
REMARK 465     PRO A  1188                                                      
REMARK 465     SER A  1189                                                      
REMARK 465     VAL A  1190                                                      
REMARK 465     VAL A  1191                                                      
REMARK 465     ARG A  1192                                                      
REMARK 465     PHE A  1193                                                      
REMARK 465     ALA A  1194                                                      
REMARK 465     VAL A  1195                                                      
REMARK 465     PRO A  1196                                                      
REMARK 465     PRO A  1197                                                      
REMARK 465     GLY A  1198                                                      
REMARK 465     HIS A  1199                                                      
REMARK 465     THR A  1200                                                      
REMARK 465     ASN A  1201                                                      
REMARK 465     ASN A  1202                                                      
REMARK 465     GLY A  1203                                                      
REMARK 465     SER A  1204                                                      
REMARK 465     ASP A  1205                                                      
REMARK 465     SER A  1206                                                      
REMARK 465     SER A  1207                                                      
REMARK 465     ASP A  1208                                                      
REMARK 465     SER A  1209                                                      
REMARK 465     GLU A  1210                                                      
REMARK 465     TYR A  1211                                                      
REMARK 465     SER A  1212                                                      
REMARK 465     SER A  1213                                                      
REMARK 465     GLN A  1214                                                      
REMARK 465     THR A  1215                                                      
REMARK 465     THR A  1216                                                      
REMARK 465     VAL A  1217                                                      
REMARK 465     SER A  1218                                                      
REMARK 465     GLY A  1219                                                      
REMARK 465     ILE A  1220                                                      
REMARK 465     SER A  1221                                                      
REMARK 465     GLU A  1222                                                      
REMARK 465     GLU A  1223                                                      
REMARK 465     LEU A  1224                                                      
REMARK 465     ARG A  1225                                                      
REMARK 465     GLN A  1226                                                      
REMARK 465     TYR A  1227                                                      
REMARK 465     GLU A  1228                                                      
REMARK 465     ALA A  1229                                                      
REMARK 465     GLN A  1230                                                      
REMARK 465     GLN A  1231                                                      
REMARK 465     GLY A  1232                                                      
REMARK 465     ALA A  1233                                                      
REMARK 465     GLY A  1234                                                      
REMARK 465     GLY A  1235                                                      
REMARK 465     PRO A  1236                                                      
REMARK 465     ALA A  1237                                                      
REMARK 465     HIS A  1238                                                      
REMARK 465     GLN A  1239                                                      
REMARK 465     VAL A  1240                                                      
REMARK 465     ILE A  1241                                                      
REMARK 465     VAL A  1242                                                      
REMARK 465     GLU A  1243                                                      
REMARK 465     ALA A  1244                                                      
REMARK 465     THR A  1245                                                      
REMARK 465     GLU A  1246                                                      
REMARK 465     ASN A  1247                                                      
REMARK 465     PRO A  1248                                                      
REMARK 465     VAL A  1249                                                      
REMARK 465     PHE A  1250                                                      
REMARK 465     ALA A  1251                                                      
REMARK 465     ARG A  1252                                                      
REMARK 465     SER A  1253                                                      
REMARK 465     THR A  1254                                                      
REMARK 465     VAL A  1255                                                      
REMARK 465     VAL A  1256                                                      
REMARK 465     HIS A  1257                                                      
REMARK 465     PRO A  1258                                                      
REMARK 465     ASP A  1259                                                      
REMARK 465     SER A  1260                                                      
REMARK 465     ARG A  1261                                                      
REMARK 465     HIS A  1262                                                      
REMARK 465     GLN A  1263                                                      
REMARK 465     PRO A  1264                                                      
REMARK 465     PRO A  1265                                                      
REMARK 465     LEU A  1266                                                      
REMARK 465     THR A  1267                                                      
REMARK 465     PRO A  1268                                                      
REMARK 465     ARG A  1269                                                      
REMARK 465     GLN A  1270                                                      
REMARK 465     GLN A  1271                                                      
REMARK 465     PRO A  1272                                                      
REMARK 465     HIS A  1273                                                      
REMARK 465     LEU A  1274                                                      
REMARK 465     ASP A  1275                                                      
REMARK 465     SER A  1276                                                      
REMARK 465     GLY A  1277                                                      
REMARK 465     SER A  1278                                                      
REMARK 465     LEU A  1279                                                      
REMARK 465     SER A  1280                                                      
REMARK 465     PRO A  1281                                                      
REMARK 465     GLY A  1282                                                      
REMARK 465     ARG A  1283                                                      
REMARK 465     GLN A  1284                                                      
REMARK 465     GLY A  1285                                                      
REMARK 465     GLN A  1286                                                      
REMARK 465     GLN A  1287                                                      
REMARK 465     PRO A  1288                                                      
REMARK 465     ARG A  1289                                                      
REMARK 465     ARG A  1290                                                      
REMARK 465     ASP A  1291                                                      
REMARK 465     MET A  1292                                                      
REMARK 465     ASP A  1293                                                      
REMARK 465     GLU A  1294                                                      
REMARK 465     LYS A  1295                                                      
REMARK 465     THR A  1296                                                      
REMARK 465     THR A  1297                                                      
REMARK 465     GLY A  1298                                                      
REMARK 465     TRP A  1299                                                      
REMARK 465     ARG A  1300                                                      
REMARK 465     GLY A  1301                                                      
REMARK 465     GLY A  1302                                                      
REMARK 465     HIS A  1303                                                      
REMARK 465     VAL A  1304                                                      
REMARK 465     VAL A  1305                                                      
REMARK 465     GLU A  1306                                                      
REMARK 465     GLY A  1307                                                      
REMARK 465     LEU A  1308                                                      
REMARK 465     ALA A  1309                                                      
REMARK 465     GLY A  1310                                                      
REMARK 465     GLU A  1311                                                      
REMARK 465     LEU A  1312                                                      
REMARK 465     GLU A  1313                                                      
REMARK 465     GLN A  1314                                                      
REMARK 465     LEU A  1315                                                      
REMARK 465     ARG A  1316                                                      
REMARK 465     ALA A  1317                                                      
REMARK 465     ARG A  1318                                                      
REMARK 465     LEU A  1319                                                      
REMARK 465     GLU A  1320                                                      
REMARK 465     HIS A  1321                                                      
REMARK 465     HIS A  1322                                                      
REMARK 465     PRO A  1323                                                      
REMARK 465     GLN A  1324                                                      
REMARK 465     GLY A  1325                                                      
REMARK 465     GLN A  1326                                                      
REMARK 465     ARG A  1327                                                      
REMARK 465     GLU A  1328                                                      
REMARK 465     PRO A  1329                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     ARG A  59    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS A  60    CG   CD   CE   NZ                                   
REMARK 470     PRO A  62    CG   CD                                             
REMARK 470     LEU A  63    CG   CD1  CD2                                       
REMARK 470     ARG A  66    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS A  68    CG   CD   CE   NZ                                   
REMARK 470     GLN A  70    CG   CD   OE1  NE2                                  
REMARK 470     ARG A  71    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LEU A  72    CG   CD1  CD2                                       
REMARK 470     LEU A  73    CG   CD1  CD2                                       
REMARK 470     LYS A  75    CG   CD   CE   NZ                                   
REMARK 470     LEU A  76    CG   CD1  CD2                                       
REMARK 470     CYS A  78    SG                                                  
REMARK 470     TYR A  79    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     ILE A  80    CG1  CG2  CD1                                       
REMARK 470     GLN A  81    CG   CD   OE1  NE2                                  
REMARK 470     LYS A  82    CG   CD   CE   NZ                                   
REMARK 470     ASN A  83    CG   OD1  ND2                                       
REMARK 470     CYS A  84    SG                                                  
REMARK 470     LYS A  86    CG   CD   CE   NZ                                   
REMARK 470     PHE A  87    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU A  88    CG   CD1  CD2                                       
REMARK 470     VAL A  89    CG1  CG2                                            
REMARK 470     VAL A  90    CG1  CG2                                            
REMARK 470     LEU A  92    CG   CD1  CD2                                       
REMARK 470     LEU A  93    CG   CD1  CD2                                       
REMARK 470     ILE A  94    CG1  CG2  CD1                                       
REMARK 470     PHE A  95    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     VAL A 100    CG1  CG2                                            
REMARK 470     LEU A 102    CG   CD1  CD2                                       
REMARK 470     LYS A 103    CG   CD   CE   NZ                                   
REMARK 470     ASN A 106    CG   OD1  ND2                                       
REMARK 470     GLU A 108    CG   CD   OE1  OE2                                  
REMARK 470     ASN A 110    CG   OD1  ND2                                       
REMARK 470     GLU A 112    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 113    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 135    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 136    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 150    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 151    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 159    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 181    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 194    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 198    CG   CD   OE1  OE2                                  
REMARK 470     ASP A 203    CG   OD1  OD2                                       
REMARK 470     GLU A 207    CG   CD   OE1  OE2                                  
REMARK 470     SER A 229    OG                                                  
REMARK 470     LYS A 237    CG   CD   CE   NZ                                   
REMARK 470     GLU A 253    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 255    CG   CD   CE   NZ                                   
REMARK 470     LYS A 256    CG   CD   CE   NZ                                   
REMARK 470     ASN A 258    CG   OD1  ND2                                       
REMARK 470     GLN A 260    CG   CD   OE1  NE2                                  
REMARK 470     ASP A 262    CG   OD1  OD2                                       
REMARK 470     GLU A 272    CG   CD   OE1  OE2                                  
REMARK 470     ASP A 279    CG   OD1  OD2                                       
REMARK 470     ASP A 287    CG   OD1  OD2                                       
REMARK 470     ASP A 289    CG   OD1  OD2                                       
REMARK 470     THR A 293    OG1  CG2                                            
REMARK 470     LYS A 297    CG   CD   CE   NZ                                   
REMARK 470     ASN A 298    CG   OD1  ND2                                       
REMARK 470     SER A 299    OG                                                  
REMARK 470     THR A 300    OG1  CG2                                            
REMARK 470     LYS A 301    CG   CD   CE   NZ                                   
REMARK 470     ASP A 304    CG   OD1  OD2                                       
REMARK 470     LYS A 334    CG   CD   CE   NZ                                   
REMARK 470     LYS A 339    CG   CD   CE   NZ                                   
REMARK 470     SER A 342    OG                                                  
REMARK 470     LYS A 356    CG   CD   CE   NZ                                   
REMARK 470     GLU A 360    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 363    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP A 366    CG   OD1  OD2                                       
REMARK 470     SER A 369    OG                                                  
REMARK 470     ASN A 372    CG   OD1  ND2                                       
REMARK 470     ASN A 374    CG   OD1  ND2                                       
REMARK 470     GLU A 375    CG   CD   OE1  OE2                                  
REMARK 470     ASP A 376    CG   OD1  OD2                                       
REMARK 470     ARG A 377    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 383    CG   CD   OE1  OE2                                  
REMARK 470     SER A 396    OG                                                  
REMARK 470     ASN A 400    CG   OD1  ND2                                       
REMARK 470     SER A 401    OG                                                  
REMARK 470     LYS A 418    CG   CD   CE   NZ                                   
REMARK 470     SER A 421    OG                                                  
REMARK 470     ASP A 422    CG   OD1  OD2                                       
REMARK 470     SER A 424    OG                                                  
REMARK 470     ILE A 426    CG1  CG2  CD1                                       
REMARK 470     SER A 430    OG                                                  
REMARK 470     MET A 435    CG   SD   CE                                        
REMARK 470     CYS A 440    SG                                                  
REMARK 470     LEU A 441    CG   CD1  CD2                                       
REMARK 470     THR A 442    OG1  CG2                                            
REMARK 470     MET A 443    CG   SD   CE                                        
REMARK 470     LEU A 444    CG   CD1  CD2                                       
REMARK 470     ARG A 445    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     TRP A 446    CG   CD1  CD2  NE1  CE2  CE3  CZ2                   
REMARK 470     TRP A 446    CZ3  CH2                                            
REMARK 470     ASP A 447    CG   OD1  OD2                                       
REMARK 470     CYS A 448    SG                                                  
REMARK 470     SER A 449    OG                                                  
REMARK 470     LYS A 450    CG   CD   CE   NZ                                   
REMARK 470     SER A 451    OG                                                  
REMARK 470     GLN A 452    CG   CD   OE1  NE2                                  
REMARK 470     LEU A 457    CG   CD1  CD2                                       
REMARK 470     VAL A 460    CG1  CG2                                            
REMARK 470     LEU A 461    CG   CD1  CD2                                       
REMARK 470     VAL A 463    CG1  CG2                                            
REMARK 470     SER A 466    OG                                                  
REMARK 470     ILE A 477    CG1  CG2  CD1                                       
REMARK 470     SER A 480    OG                                                  
REMARK 470     ASP A 499    CG   OD1  OD2                                       
REMARK 470     ASP A 500    CG   OD1  OD2                                       
REMARK 470     VAL A 501    CG1  CG2                                            
REMARK 470     LEU A 503    CG   CD1  CD2                                       
REMARK 470     LEU A 504    CG   CD1  CD2                                       
REMARK 470     SER A 509    OG                                                  
REMARK 470     GLU A 510    CG   CD   OE1  OE2                                  
REMARK 470     THR A 511    OG1  CG2                                            
REMARK 470     GLN A 513    CG   CD   OE1  NE2                                  
REMARK 470     ASN A 514    CG   OD1  ND2                                       
REMARK 470     LYS A 515    CG   CD   CE   NZ                                   
REMARK 470     ARG A 516    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ILE A 517    CG1  CG2  CD1                                       
REMARK 470     PRO A 518    CG   CD                                             
REMARK 470     PHE A 519    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     GLU A 520    CG   CD   OE1  OE2                                  
REMARK 470     ASP A 521    CG   OD1  OD2                                       
REMARK 470     ARG A 522    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     THR A 523    OG1  CG2                                            
REMARK 470     GLU A 525    CG   CD   OE1  OE2                                  
REMARK 470     CYS A 526    SG                                                  
REMARK 470     LEU A 527    CG   CD1  CD2                                       
REMARK 470     LYS A 528    CG   CD   CE   NZ                                   
REMARK 470     THR A 530    OG1  CG2                                            
REMARK 470     SER A 533    OG                                                  
REMARK 470     VAL A 534    CG1  CG2                                            
REMARK 470     LEU A 536    CG   CD1  CD2                                       
REMARK 470     THR A 537    OG1  CG2                                            
REMARK 470     SER A 538    OG                                                  
REMARK 470     ILE A 539    CG1  CG2  CD1                                       
REMARK 470     SER A 540    OG                                                  
REMARK 470     THR A 543    OG1  CG2                                            
REMARK 470     MET A 573    CG   SD   CE                                        
REMARK 470     VAL A 574    CG1  CG2                                            
REMARK 470     ILE A 577    CG1  CG2  CD1                                       
REMARK 470     ILE A 581    CG1  CG2  CD1                                       
REMARK 470     LEU A 582    CG   CD1  CD2                                       
REMARK 470     SER A 583    OG                                                  
REMARK 470     MET A 584    CG   SD   CE                                        
REMARK 470     ASP A 585    CG   OD1  OD2                                       
REMARK 470     LEU A 586    CG   CD1  CD2                                       
REMARK 470     TYR A 587    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     ARG A 588    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG A 589    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 590    CG   CD   OE1  OE2                                  
REMARK 470     TRP A 716    CG   CD1  CD2  NE1  CE2  CE3  CZ2                   
REMARK 470     TRP A 716    CZ3  CH2                                            
REMARK 470     THR A 717    OG1  CG2                                            
REMARK 470     LEU A 718    CG   CD1  CD2                                       
REMARK 470     SER A 719    OG                                                  
REMARK 470     SER A 720    OG                                                  
REMARK 470     PHE A 721    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     GLU A 723    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 724    CG   CD   CE   NZ                                   
REMARK 470     HIS A 725    CG   ND1  CD2  CE1  NE2                             
REMARK 470     TYR A 726    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     PRO A 728    CG   CD                                             
REMARK 470     PHE A 729    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU A 730    CG   CD1  CD2                                       
REMARK 470     LEU A 731    CG   CD1  CD2                                       
REMARK 470     LYS A 732    CG   CD   CE   NZ                                   
REMARK 470     PRO A 733    CG   CD                                             
REMARK 470     LYS A 734    CG   CD   CE   NZ                                   
REMARK 470     LYS A 736    CG   CD   CE   NZ                                   
REMARK 470     VAL A 737    CG1  CG2                                            
REMARK 470     VAL A 738    CG1  CG2                                            
REMARK 470     VAL A 739    CG1  CG2                                            
REMARK 470     ILE A 740    CG1  CG2  CD1                                       
REMARK 470     LEU A 741    CG   CD1  CD2                                       
REMARK 470     LEU A 742    CG   CD1  CD2                                       
REMARK 470     LEU A 744    CG   CD1  CD2                                       
REMARK 470     LEU A 746    CG   CD1  CD2                                       
REMARK 470     VAL A 749    CG1  CG2                                            
REMARK 470     SER A 750    OG                                                  
REMARK 470     THR A 755    OG1  CG2                                            
REMARK 470     ARG A 758    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP A 759    CG   OD1  OD2                                       
REMARK 470     ASP A 762    CG   OD1  OD2                                       
REMARK 470     ASP A 765    CG   OD1  OD2                                       
REMARK 470     ARG A 769    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 770    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 772    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 773    CG   CD   OE1  OE2                                  
REMARK 470     LYS A 782    CG   CD   CE   NZ                                   
REMARK 470     ASP A 797    CG   OD1  OD2                                       
REMARK 470     ASP A 807    CG   OD1  OD2                                       
REMARK 470     LYS A 810    CG   CD   CE   NZ                                   
REMARK 470     SER A 811    OG                                                  
REMARK 470     SER A 813    OG                                                  
REMARK 470     LYS A 816    CG   CD   CE   NZ                                   
REMARK 470     GLU A 821    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 822    CG   CD   OE1  OE2                                  
REMARK 470     ASN A 823    CG   OD1  ND2                                       
REMARK 470     LYS A 824    CG   CD   CE   NZ                                   
REMARK 470     GLN A 825    CG   CD   OE1  NE2                                  
REMARK 470     ASP A 846    CG   OD1  OD2                                       
REMARK 470     SER A 847    OG                                                  
REMARK 470     ASP A 848    CG   OD1  OD2                                       
REMARK 470     GLU A 850    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 853    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ILE A 854    CG1  CG2  CD1                                       
REMARK 470     MET A 855    CG   SD   CE                                        
REMARK 470     ASN A 857    CG   OD1  ND2                                       
REMARK 470     ASN A 858    CG   OD1  ND2                                       
REMARK 470     LYS A 860    CG   CD   CE   NZ                                   
REMARK 470     SER A 863    OG                                                  
REMARK 470     ASP A 864    CG   OD1  OD2                                       
REMARK 470     ASP A 865    CG   OD1  OD2                                       
REMARK 470     THR A 876    OG1  CG2                                            
REMARK 470     SER A 878    OG                                                  
REMARK 470     ARG A 879    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP A 880    CG   OD1  OD2                                       
REMARK 470     LYS A 881    CG   CD   CE   NZ                                   
REMARK 470     ILE A 883    CG1  CG2  CD1                                       
REMARK 470     ASP A 884    CG   OD1  OD2                                       
REMARK 470     SER A 886    OG                                                  
REMARK 470     GLN A 887    CG   CD   OE1  NE2                                  
REMARK 470     LYS A 890    CG   CD   CE   NZ                                   
REMARK 470     GLN A 891    CG   CD   OE1  NE2                                  
REMARK 470     ARG A 892    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP A 895    CG   OD1  OD2                                       
REMARK 470     ASP A 897    CG   OD1  OD2                                       
REMARK 470     SER A 903    OG                                                  
REMARK 470     GLU A 933    CG   CD   OE1  OE2                                  
REMARK 470     ASP A 937    CG   OD1  OD2                                       
REMARK 470     LYS A 938    CG   CD   CE   NZ                                   
REMARK 470     ASP A 940    CG   OD1  OD2                                       
REMARK 470     MET A 942    CG   SD   CE                                        
REMARK 470     GLU A 944    CG   CD   OE1  OE2                                  
REMARK 470     THR A 945    OG1  CG2                                            
REMARK 470     ARG A 948    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 953    CG   CD   OE1  OE2                                  
REMARK 470     SER A 989    OG                                                  
REMARK 470     SER A 993    OG                                                  
REMARK 470     SER A 994    OG                                                  
REMARK 470     SER A1009    OG                                                  
REMARK 470     PHE A1032    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     LEU A1033    CG   CD1  CD2                                       
REMARK 470     LEU A1034    CG   CD1  CD2                                       
REMARK 470     ASN A1035    CG   OD1  ND2                                       
REMARK 470     MET A1044    CG   SD   CE                                        
REMARK 470     GLU A1052    CG   CD   OE1  OE2                                  
REMARK 470     SER A1065    OG                                                  
REMARK 470     GLU A1081    CG   CD   OE1  OE2                                  
REMARK 470     ASP A1096    CG   OD1  OD2                                       
REMARK 470     LYS A1097    CG   CD   CE   NZ                                   
REMARK 470     ASN A1098    CG   OD1  ND2                                       
REMARK 470     ARG A1100    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     MET A1102    CG   SD   CE                                        
REMARK 470     GLU A1106    CG   CD   OE1  OE2                                  
REMARK 470     ASP A1114    CG   OD1  OD2                                       
REMARK 470     SER A1118    OG                                                  
REMARK 470     SER A1129    OG                                                  
REMARK 470     GLU A1130    CG   CD   OE1  OE2                                  
REMARK 470     ASP A1132    CG   OD1  OD2                                       
REMARK 470     SER A1162    OG                                                  
REMARK 470     PHE A1164    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     CYS A1167    SG                                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   CB   ALA A   160     OE1  GLN A   403              1.18            
REMARK 500   CA   ALA A   160     OE1  GLN A   403              1.20            
REMARK 500   CD1  ILE A   206     CAV  UNL A  1401              1.28            
REMARK 500   CD1  LEU A   462     CE1  PHE A   578              1.66            
REMARK 500   OE1  GLN A   839     CG1  VAL A   894              1.73            
REMARK 500   CD1  ILE A   900     CE1  PHE A   905              1.74            
REMARK 500   OG   SER A   475     CE1  PHE A   481              1.77            
REMARK 500   CD2  PHE A  1003     CAX  UNL A  1402              1.84            
REMARK 500   O    ALA A   160     NE2  GLN A   403              1.88            
REMARK 500   CD2  HIS A  1085     CE   MET A  1108              1.93            
REMARK 500   CD2  LEU A   268     CAX  UNL A  1401              1.95            
REMARK 500   OH   TYR A   210     CAB  UNL A  1401              1.96            
REMARK 500   CD1  ILE A   206     CAO  UNL A  1401              1.96            
REMARK 500   CD1  ILE A  1042     CG2  THR A  1083              1.97            
REMARK 500   OE1  GLN A   802     CD1  ILE A   899              1.99            
REMARK 500   CG1  VAL A   423     NE2  GLN A   487              2.01            
REMARK 500   OE1  GLN A   839     CG2  VAL A   894              2.04            
REMARK 500   C    ALA A   160     OE1  GLN A   403              2.04            
REMARK 500   CD1  LEU A   462     CD1  PHE A   578              2.06            
REMARK 500   O    ASP A   759     O    LEU A  1064              2.06            
REMARK 500   CB   ALA A   469     CE2  PHE A   569              2.07            
REMARK 500   CB   ALA A   160     CD   GLN A   403              2.09            
REMARK 500   O    HIS A  1099     N    LEU A  1103              2.10            
REMARK 500   CD1  ILE A   900     CD1  PHE A   905              2.13            
REMARK 500   CB   LEU A   462     CE1  PHE A   578              2.13            
REMARK 500   O    ALA A  1089     N    ALA A  1093              2.14            
REMARK 500   OG   SER A   475     CZ   PHE A   481              2.15            
REMARK 500   NZ   LYS A   132     OE1  GLU A   956              2.18            
REMARK 500   CB   LEU A   462     CZ   PHE A   578              2.18            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    MET A 144      112.03   -162.35                                   
REMARK 500    SER A 229       61.64     61.76                                   
REMARK 500    ASN A 258       65.48     60.50                                   
REMARK 500    CYS A 290       77.62     43.65                                   
REMARK 500    THR A 300     -166.23   -122.86                                   
REMARK 500    LEU A 309       30.69    -98.52                                   
REMARK 500    VAL A 341      -59.22   -120.87                                   
REMARK 500    HIS A 344      139.26   -170.61                                   
REMARK 500    ALA A 345       70.65     60.20                                   
REMARK 500    PHE A 408      147.58   -172.48                                   
REMARK 500    ASP A 759     -130.49     57.08                                   
REMARK 500    ARG A 892       73.94     51.29                                   
REMARK 500    ASP A 895     -134.80     55.31                                   
REMARK 500    ALA A 896      -38.88   -140.07                                   
REMARK 500    ASP A 916       70.75   -151.02                                   
REMARK 500    GLU A 933       64.35     61.51                                   
REMARK 500    LYS A 938       -3.56     83.20                                   
REMARK 500    ILE A 949       53.45     33.18                                   
REMARK 500    ALA A 958      117.39   -162.45                                   
REMARK 500    LEU A 967      -67.15    -94.62                                   
REMARK 500    ARG A 968       -6.61     65.86                                   
REMARK 500    VAL A1155      -57.35   -120.95                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 600                                                                      
REMARK 600 HETEROGEN                                                            
REMARK 600                                                                      
REMARK 600 THE AUTHORS STATE THAT THE EXACT IDENTITY OF THE LIGAND IS UNKNOWN.  
REMARK 600 CHOLESTEROL WAS MODELED INTO THE CHOLESTEROL-LIKE DENSITY PRESENT    
REMARK 600 IN THE MAP.                                                          
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-9111   RELATED DB: EMDB                              
REMARK 900 STRUCTURAL BASIS FOR CHOLESTEROL TRANSPORT-LIKE ACTIVITY OF THE      
REMARK 900 HEDGEHOG RECEPTOR PATCHED                                            
DBREF  6MG8 A    1   643  UNP    Q61115   PTC1_MOUSE       1    619             
DBREF  6MG8 A  644  1291  UNP    Q61115   PTC1_MOUSE     644   1291             
DBREF  6MG8 A 1292  1329  PDB    6MG8     6MG8          1292   1329             
SEQRES   1 A 1305  MET ALA SER ALA GLY ASN ALA ALA GLY ALA LEU GLY ARG          
SEQRES   2 A 1305  GLN ALA GLY GLY GLY ARG ARG ARG ARG THR GLY GLY PRO          
SEQRES   3 A 1305  HIS ARG ALA ALA PRO ASP ARG ASP TYR LEU HIS ARG PRO          
SEQRES   4 A 1305  SER TYR CYS ASP ALA ALA PHE ALA LEU GLU GLN ILE SER          
SEQRES   5 A 1305  LYS GLY LYS ALA THR GLY ARG LYS ALA PRO LEU TRP LEU          
SEQRES   6 A 1305  ARG ALA LYS PHE GLN ARG LEU LEU PHE LYS LEU GLY CYS          
SEQRES   7 A 1305  TYR ILE GLN LYS ASN CYS GLY LYS PHE LEU VAL VAL GLY          
SEQRES   8 A 1305  LEU LEU ILE PHE GLY ALA PHE ALA VAL GLY LEU LYS ALA          
SEQRES   9 A 1305  ALA ASN LEU GLU THR ASN VAL GLU GLU LEU TRP VAL GLU          
SEQRES  10 A 1305  VAL GLY GLY ARG VAL SER ARG GLU LEU ASN TYR THR ARG          
SEQRES  11 A 1305  GLN LYS ILE GLY GLU GLU ALA MET PHE ASN PRO GLN LEU          
SEQRES  12 A 1305  MET ILE GLN THR PRO LYS GLU GLU GLY ALA ASN VAL LEU          
SEQRES  13 A 1305  THR THR GLU ALA LEU LEU GLN HIS LEU ASP SER ALA LEU          
SEQRES  14 A 1305  GLN ALA SER ARG VAL HIS VAL TYR MET TYR ASN ARG GLN          
SEQRES  15 A 1305  TRP LYS LEU GLU HIS LEU CYS TYR LYS SER GLY GLU LEU          
SEQRES  16 A 1305  ILE THR GLU THR GLY TYR MET ASP GLN ILE ILE GLU TYR          
SEQRES  17 A 1305  LEU TYR PRO CYS LEU ILE ILE THR PRO LEU ASP CYS PHE          
SEQRES  18 A 1305  TRP GLU GLY ALA LYS LEU GLN SER GLY THR ALA TYR LEU          
SEQRES  19 A 1305  LEU GLY LYS PRO PRO LEU ARG TRP THR ASN PHE ASP PRO          
SEQRES  20 A 1305  LEU GLU PHE LEU GLU GLU LEU LYS LYS ILE ASN TYR GLN          
SEQRES  21 A 1305  VAL ASP SER TRP GLU GLU MET LEU ASN LYS ALA GLU VAL          
SEQRES  22 A 1305  GLY HIS GLY TYR MET ASP ARG PRO CYS LEU ASN PRO ALA          
SEQRES  23 A 1305  ASP PRO ASP CYS PRO ALA THR ALA PRO ASN LYS ASN SER          
SEQRES  24 A 1305  THR LYS PRO LEU ASP VAL ALA LEU VAL LEU ASN GLY GLY          
SEQRES  25 A 1305  CYS GLN GLY LEU SER ARG LYS TYR MET HIS TRP GLN GLU          
SEQRES  26 A 1305  GLU LEU ILE VAL GLY GLY THR VAL LYS ASN ALA THR GLY          
SEQRES  27 A 1305  LYS LEU VAL SER ALA HIS ALA LEU GLN THR MET PHE GLN          
SEQRES  28 A 1305  LEU MET THR PRO LYS GLN MET TYR GLU HIS PHE ARG GLY          
SEQRES  29 A 1305  TYR ASP TYR VAL SER HIS ILE ASN TRP ASN GLU ASP ARG          
SEQRES  30 A 1305  ALA ALA ALA ILE LEU GLU ALA TRP GLN ARG THR TYR VAL          
SEQRES  31 A 1305  GLU VAL VAL HIS GLN SER VAL ALA PRO ASN SER THR GLN          
SEQRES  32 A 1305  LYS VAL LEU PRO PHE THR THR THR THR LEU ASP ASP ILE          
SEQRES  33 A 1305  LEU LYS SER PHE SER ASP VAL SER VAL ILE ARG VAL ALA          
SEQRES  34 A 1305  SER GLY TYR LEU LEU MET LEU ALA TYR ALA CYS LEU THR          
SEQRES  35 A 1305  MET LEU ARG TRP ASP CYS SER LYS SER GLN GLY ALA VAL          
SEQRES  36 A 1305  GLY LEU ALA GLY VAL LEU LEU VAL ALA LEU SER VAL ALA          
SEQRES  37 A 1305  ALA GLY LEU GLY LEU CYS SER LEU ILE GLY ILE SER PHE          
SEQRES  38 A 1305  ASN ALA ALA THR THR GLN VAL LEU PRO PHE LEU ALA LEU          
SEQRES  39 A 1305  GLY VAL GLY VAL ASP ASP VAL PHE LEU LEU ALA HIS ALA          
SEQRES  40 A 1305  PHE SER GLU THR GLY GLN ASN LYS ARG ILE PRO PHE GLU          
SEQRES  41 A 1305  ASP ARG THR GLY GLU CYS LEU LYS ARG THR GLY ALA SER          
SEQRES  42 A 1305  VAL ALA LEU THR SER ILE SER ASN VAL THR ALA PHE PHE          
SEQRES  43 A 1305  MET ALA ALA LEU ILE PRO ILE PRO ALA LEU ARG ALA PHE          
SEQRES  44 A 1305  SER LEU GLN ALA ALA VAL VAL VAL VAL PHE ASN PHE ALA          
SEQRES  45 A 1305  MET VAL LEU LEU ILE PHE PRO ALA ILE LEU SER MET ASP          
SEQRES  46 A 1305  LEU TYR ARG ARG GLU ASP ARG ARG LEU ASP ILE PHE CYS          
SEQRES  47 A 1305  CYS PHE THR SER PRO CYS VAL SER ARG VAL ILE GLN VAL          
SEQRES  48 A 1305  GLU PRO GLN ALA TYR THR GLU PRO MET GLN SER THR VAL          
SEQRES  49 A 1305  GLN LEU ARG THR GLU TYR ASP PRO HIS THR HIS VAL TYR          
SEQRES  50 A 1305  TYR THR THR ALA GLU PRO ARG SER GLU ILE SER VAL GLN          
SEQRES  51 A 1305  PRO VAL THR VAL THR GLN ASP ASN LEU SER CYS GLN SER          
SEQRES  52 A 1305  PRO GLU SER THR SER SER THR ARG ASP LEU LEU SER GLN          
SEQRES  53 A 1305  PHE SER ASP SER SER LEU HIS CYS LEU GLU PRO PRO CYS          
SEQRES  54 A 1305  THR LYS TRP THR LEU SER SER PHE ALA GLU LYS HIS TYR          
SEQRES  55 A 1305  ALA PRO PHE LEU LEU LYS PRO LYS ALA LYS VAL VAL VAL          
SEQRES  56 A 1305  ILE LEU LEU PHE LEU GLY LEU LEU GLY VAL SER LEU TYR          
SEQRES  57 A 1305  GLY THR THR ARG VAL ARG ASP GLY LEU ASP LEU THR ASP          
SEQRES  58 A 1305  ILE VAL PRO ARG GLU THR ARG GLU TYR ASP PHE ILE ALA          
SEQRES  59 A 1305  ALA GLN PHE LYS TYR PHE SER PHE TYR ASN MET TYR ILE          
SEQRES  60 A 1305  VAL THR GLN LYS ALA ASP TYR PRO ASN ILE GLN HIS LEU          
SEQRES  61 A 1305  LEU TYR ASP LEU HIS LYS SER PHE SER ASN VAL LYS TYR          
SEQRES  62 A 1305  VAL MET LEU GLU GLU ASN LYS GLN LEU PRO GLN MET TRP          
SEQRES  63 A 1305  LEU HIS TYR PHE ARG ASP TRP LEU GLN GLY LEU GLN ASP          
SEQRES  64 A 1305  ALA PHE ASP SER ASP TRP GLU THR GLY ARG ILE MET PRO          
SEQRES  65 A 1305  ASN ASN TYR LYS ASN GLY SER ASP ASP GLY VAL LEU ALA          
SEQRES  66 A 1305  TYR LYS LEU LEU VAL GLN THR GLY SER ARG ASP LYS PRO          
SEQRES  67 A 1305  ILE ASP ILE SER GLN LEU THR LYS GLN ARG LEU VAL ASP          
SEQRES  68 A 1305  ALA ASP GLY ILE ILE ASN PRO SER ALA PHE TYR ILE TYR          
SEQRES  69 A 1305  LEU THR ALA TRP VAL SER ASN ASP PRO VAL ALA TYR ALA          
SEQRES  70 A 1305  ALA SER GLN ALA ASN ILE ARG PRO HIS ARG PRO GLU TRP          
SEQRES  71 A 1305  VAL HIS ASP LYS ALA ASP TYR MET PRO GLU THR ARG LEU          
SEQRES  72 A 1305  ARG ILE PRO ALA ALA GLU PRO ILE GLU TYR ALA GLN PHE          
SEQRES  73 A 1305  PRO PHE TYR LEU ASN GLY LEU ARG ASP THR SER ASP PHE          
SEQRES  74 A 1305  VAL GLU ALA ILE GLU LYS VAL ARG VAL ILE CYS ASN ASN          
SEQRES  75 A 1305  TYR THR SER LEU GLY LEU SER SER TYR PRO ASN GLY TYR          
SEQRES  76 A 1305  PRO PHE LEU PHE TRP GLU GLN TYR ILE SER LEU ARG HIS          
SEQRES  77 A 1305  TRP LEU LEU LEU SER ILE SER VAL VAL LEU ALA CYS THR          
SEQRES  78 A 1305  PHE LEU VAL CYS ALA VAL PHE LEU LEU ASN PRO TRP THR          
SEQRES  79 A 1305  ALA GLY ILE ILE VAL MET VAL LEU ALA LEU MET THR VAL          
SEQRES  80 A 1305  GLU LEU PHE GLY MET MET GLY LEU ILE GLY ILE LYS LEU          
SEQRES  81 A 1305  SER ALA VAL PRO VAL VAL ILE LEU ILE ALA SER VAL GLY          
SEQRES  82 A 1305  ILE GLY VAL GLU PHE THR VAL HIS VAL ALA LEU ALA PHE          
SEQRES  83 A 1305  LEU THR ALA ILE GLY ASP LYS ASN HIS ARG ALA MET LEU          
SEQRES  84 A 1305  ALA LEU GLU HIS MET PHE ALA PRO VAL LEU ASP GLY ALA          
SEQRES  85 A 1305  VAL SER THR LEU LEU GLY VAL LEU MET LEU ALA GLY SER          
SEQRES  86 A 1305  GLU PHE ASP PHE ILE VAL ARG TYR PHE PHE ALA VAL LEU          
SEQRES  87 A 1305  ALA ILE LEU THR VAL LEU GLY VAL LEU ASN GLY LEU VAL          
SEQRES  88 A 1305  LEU LEU PRO VAL LEU LEU SER PHE PHE GLY PRO CYS PRO          
SEQRES  89 A 1305  GLU VAL SER PRO ALA ASN GLY LEU ASN ARG LEU PRO THR          
SEQRES  90 A 1305  PRO SER PRO GLU PRO PRO PRO SER VAL VAL ARG PHE ALA          
SEQRES  91 A 1305  VAL PRO PRO GLY HIS THR ASN ASN GLY SER ASP SER SER          
SEQRES  92 A 1305  ASP SER GLU TYR SER SER GLN THR THR VAL SER GLY ILE          
SEQRES  93 A 1305  SER GLU GLU LEU ARG GLN TYR GLU ALA GLN GLN GLY ALA          
SEQRES  94 A 1305  GLY GLY PRO ALA HIS GLN VAL ILE VAL GLU ALA THR GLU          
SEQRES  95 A 1305  ASN PRO VAL PHE ALA ARG SER THR VAL VAL HIS PRO ASP          
SEQRES  96 A 1305  SER ARG HIS GLN PRO PRO LEU THR PRO ARG GLN GLN PRO          
SEQRES  97 A 1305  HIS LEU ASP SER GLY SER LEU SER PRO GLY ARG GLN GLY          
SEQRES  98 A 1305  GLN GLN PRO ARG ARG ASP MET ASP GLU LYS THR THR GLY          
SEQRES  99 A 1305  TRP ARG GLY GLY HIS VAL VAL GLU GLY LEU ALA GLY GLU          
SEQRES 100 A 1305  LEU GLU GLN LEU ARG ALA ARG LEU GLU HIS HIS PRO GLN          
SEQRES 101 A 1305  GLY GLN ARG GLU PRO                                          
HET    UNL  A1401      28                                                       
HET    UNL  A1402      28                                                       
HET    UNL  A1403      28                                                       
HET    UNL  A1404      28                                                       
HETNAM     UNL UNKNOWN LIGAND                                                   
HELIX    1 AA1 ARG A   59  CYS A   84  1                                  26    
HELIX    2 AA2 GLY A   85  VAL A  100  1                                  16    
HELIX    3 AA3 GLY A  101  ALA A  104  5                                   4    
HELIX    4 AA4 ASN A  110  TRP A  115  1                                   6    
HELIX    5 AA5 GLY A  120  ILE A  133  1                                  14    
HELIX    6 AA6 LEU A  161  ARG A  173  1                                  13    
HELIX    7 AA7 LYS A  184  LEU A  188  5                                   5    
HELIX    8 AA8 TYR A  201  LEU A  209  1                                   9    
HELIX    9 AA9 PHE A  221  ALA A  225  5                                   5    
HELIX   10 AB1 ASP A  246  ASN A  258  1                                  13    
HELIX   11 AB2 VAL A  261  ALA A  271  1                                  11    
HELIX   12 AB3 ASP A  304  LEU A  309  1                                   6    
HELIX   13 AB4 THR A  354  PHE A  362  1                                   9    
HELIX   14 AB5 ASN A  374  VAL A  397  1                                  24    
HELIX   15 AB6 THR A  411  ASP A  422  1                                  12    
HELIX   16 AB7 SER A  424  MET A  443  1                                  20    
HELIX   17 AB8 LEU A  444  TRP A  446  5                                   3    
HELIX   18 AB9 GLN A  452  GLY A  478  1                                  27    
HELIX   19 AC1 ALA A  484  ASN A  514  1                                  31    
HELIX   20 AC2 ASP A  521  CYS A  526  1                                   6    
HELIX   21 AC3 LEU A  527  ALA A  548  1                                  22    
HELIX   22 AC4 ALA A  549  ILE A  551  5                                   3    
HELIX   23 AC5 ILE A  553  GLU A  590  1                                  38    
HELIX   24 AC6 SER A  720  ALA A  727  1                                   8    
HELIX   25 AC7 LYS A  732  ARG A  756  1                                  25    
HELIX   26 AC8 THR A  771  PHE A  784  1                                  14    
HELIX   27 AC9 ASP A  797  ASN A  800  5                                   4    
HELIX   28 AD1 ILE A  801  SER A  811  1                                  11    
HELIX   29 AD2 TRP A  830  GLY A  852  1                                  23    
HELIX   30 AD3 SER A  863  GLN A  875  1                                  13    
HELIX   31 AD4 SER A  886  ARG A  892  1                                   7    
HELIX   32 AD5 ALA A  904  ASP A  916  1                                  13    
HELIX   33 AD6 ASP A  916  SER A  923  1                                   8    
HELIX   34 AD7 MET A  942  LEU A  947  1                                   6    
HELIX   35 AD8 ASP A  969  ASN A  986  1                                  18    
HELIX   36 AD9 GLY A  998  TRP A 1004  1                                   7    
HELIX   37 AE1 GLU A 1005  LEU A 1034  1                                  30    
HELIX   38 AE2 ASN A 1035  GLY A 1061  1                                  27    
HELIX   39 AE3 ALA A 1066  ILE A 1094  1                                  29    
HELIX   40 AE4 ASN A 1098  PHE A 1109  1                                  12    
HELIX   41 AE5 PHE A 1109  ASP A 1114  1                                   6    
HELIX   42 AE6 ALA A 1116  ALA A 1127  1                                  12    
HELIX   43 AE7 ASP A 1132  VAL A 1155  1                                  24    
HELIX   44 AE8 VAL A 1155  PHE A 1164  1                                  10    
SHEET    1 AA1 3 LEU A 346  LEU A 352  0                                        
SHEET    2 AA1 3 ASN A 140  GLN A 146 -1  N  ASN A 140   O  LEU A 352           
SHEET    3 AA1 3 LEU A 406  PRO A 407 -1  O  LEU A 406   N  ILE A 145           
SHEET    1 AA2 2 VAL A 176  MET A 178  0                                        
SHEET    2 AA2 2 ARG A 181  TRP A 183 -1  O  ARG A 181   N  MET A 178           
SHEET    1 AA3 2 THR A 231  ALA A 232  0                                        
SHEET    2 AA3 2 LEU A 240  ARG A 241 -1  O  LEU A 240   N  ALA A 232           
SHEET    1 AA4 2 VAL A 333  LYS A 334  0                                        
SHEET    2 AA4 2 LEU A 340  SER A 342 -1  O  SER A 342   N  VAL A 333           
SHEET    1 AA5 3 ALA A 958  TYR A 963  0                                        
SHEET    2 AA5 3 ASN A 788  THR A 793 -1  N  MET A 789   O  PHE A 962           
SHEET    3 AA5 3 PRO A 996  ASN A 997 -1  O  ASN A 997   N  TYR A 790           
SSBOND   1 CYS A  189    CYS A  212                          1555   1555  2.03  
SSBOND   2 CYS A  220    CYS A  313                          1555   1555  2.02  
SSBOND   3 CYS A  282    CYS A  290                          1555   1555  2.04  
CISPEP   1 ARG A  928    PRO A  929          0        -7.45                     
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1                      
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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