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Database: PDB
Entry: 6PRK
LinkDB: 6PRK
Original site: 6PRK 
HEADER    METAL BINDING PROTEIN                   10-JUL-19   6PRK              
TITLE     X-RAY CRYSTAL STRUCTURE OF BACILLUS SUBTILIS RICA IN COMPLEX WITH RICF
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: RICF;                                                      
COMPND   3 CHAIN: A;                                                            
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: RICA;                                                      
COMPND   7 CHAIN: B;                                                            
COMPND   8 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: BACILLUS SUBTILIS (STRAIN 168);                 
SOURCE   3 ORGANISM_TAXID: 224308;                                              
SOURCE   4 STRAIN: 168;                                                         
SOURCE   5 GENE: YLBF, BSU14990;                                                
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: BACILLUS SUBTILIS (STRAIN 168);                 
SOURCE  10 ORGANISM_TAXID: 224308;                                              
SOURCE  11 STRAIN: 168;                                                         
SOURCE  12 GENE: YMCA, BSU17020;                                                
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    RNA PROCESSING, BIOFILMS, COMPETENCE, SPORULATION, METAL BINDING      
KEYWDS   2 PROTEIN                                                              
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    F.T.KHAJA,P.D.JEFFREY,M.B.NEIDITCH,D.DUBNAU                           
REVDAT   2   18-DEC-19 6PRK    1       REMARK                                   
REVDAT   1   02-OCT-19 6PRK    0                                                
JRNL        AUTH   F.ADUSEI-DANSO,F.T.KHAJA,M.DESANTIS,P.D.JEFFREY,E.DUBNAU,    
JRNL        AUTH 2 B.DEMELER,M.B.NEIDITCH,D.DUBNAU                              
JRNL        TITL   STRUCTURE-FUNCTION STUDIES OF THE BACILLUS SUBTILIS RIC      
JRNL        TITL 2 PROTEINS IDENTIFY THE FE-S CLUSTER-LIGATING RESIDUES AND     
JRNL        TITL 3 THEIR ROLES IN DEVELOPMENT AND RNA PROCESSING.               
JRNL        REF    MBIO                          V.  10       2019              
JRNL        REFN                   ESSN 2150-7511                               
JRNL        PMID   31530674                                                     
JRNL        DOI    10.1128/MBIO.01841-19                                        
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.20 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : BUSTER 2.10.3                                        
REMARK   3   AUTHORS     : BRICOGNE,BLANC,BRANDL,FLENSBURG,KELLER,              
REMARK   3               : PACIOREK,ROVERSI,SHARFF,SMART,VONRHEIN,              
REMARK   3               : WOMACK,MATTHEWS,TEN EYCK,TRONRUD                     
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.20                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 43.04                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 100.0                          
REMARK   3   NUMBER OF REFLECTIONS             : 6149                           
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD           : THROUGHOUT                     
REMARK   3   FREE R VALUE TEST SET SELECTION   : RANDOM                         
REMARK   3   R VALUE     (WORKING + TEST SET)  : 0.209                          
REMARK   3   R VALUE            (WORKING SET)  : 0.205                          
REMARK   3   FREE R VALUE                      : 0.278                          
REMARK   3   FREE R VALUE TEST SET SIZE   (%)  : 4.830                          
REMARK   3   FREE R VALUE TEST SET COUNT       : 297                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE   : NULL                           
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED               : 5                        
REMARK   3   BIN RESOLUTION RANGE HIGH   (ANGSTROMS) : 3.20                     
REMARK   3   BIN RESOLUTION RANGE LOW    (ANGSTROMS) : 3.58                     
REMARK   3   BIN COMPLETENESS (WORKING+TEST)     (%) : 99.83                    
REMARK   3   REFLECTIONS IN BIN (WORKING + TEST SET) : 1726                     
REMARK   3   BIN R VALUE        (WORKING + TEST SET) : 0.3280                   
REMARK   3   REFLECTIONS IN BIN        (WORKING SET) : 1641                     
REMARK   3   BIN R VALUE               (WORKING SET) : 0.3250                   
REMARK   3   BIN FREE R VALUE                        : 0.3990                   
REMARK   3   BIN FREE R VALUE TEST SET SIZE      (%) : 4.92                     
REMARK   3   BIN FREE R VALUE TEST SET COUNT         : 85                       
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE     : 0.000                    
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 1905                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 0                                       
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 105.4                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 144.7                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -5.29150                                             
REMARK   3    B22 (A**2) : -5.29150                                             
REMARK   3    B33 (A**2) : 10.58300                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT                    (A) : 0.410               
REMARK   3   DPI (BLOW EQ-10) BASED ON R VALUE        (A) : NULL                
REMARK   3   DPI (BLOW EQ-9) BASED ON FREE R VALUE    (A) : 0.475               
REMARK   3   DPI (CRUICKSHANK) BASED ON R VALUE       (A) : NULL                
REMARK   3   DPI (CRUICKSHANK) BASED ON FREE R VALUE  (A) : NULL                
REMARK   3                                                                      
REMARK   3   REFERENCES: BLOW, D. (2002) ACTA CRYST D58, 792-797                
REMARK   3               CRUICKSHANK, D.W.J. (1999) ACTA CRYST D55, 583-601     
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.961                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.944                         
REMARK   3                                                                      
REMARK   3   NUMBER OF GEOMETRIC FUNCTION TERMS DEFINED : 15                    
REMARK   3   TERM                          COUNT    WEIGHT   FUNCTION.          
REMARK   3    BOND LENGTHS              : 1923   ; 2.000  ; HARMONIC            
REMARK   3    BOND ANGLES               : 2581   ; 2.000  ; HARMONIC            
REMARK   3    TORSION ANGLES            : 746    ; 2.000  ; SINUSOIDAL          
REMARK   3    TRIGONAL CARBON PLANES    : 78     ; 2.000  ; HARMONIC            
REMARK   3    GENERAL PLANES            : 262    ; 5.000  ; HARMONIC            
REMARK   3    ISOTROPIC THERMAL FACTORS : 1923   ; 20.000 ; HARMONIC            
REMARK   3    BAD NON-BONDED CONTACTS   : NULL   ; NULL   ; NULL                
REMARK   3    IMPROPER TORSIONS         : NULL   ; NULL   ; NULL                
REMARK   3    PSEUDOROTATION ANGLES     : NULL   ; NULL   ; NULL                
REMARK   3    CHIRAL IMPROPER TORSION   : 256    ; 5.000  ; SEMIHARMONIC        
REMARK   3    SUM OF OCCUPANCIES        : NULL   ; NULL   ; NULL                
REMARK   3    UTILITY DISTANCES         : NULL   ; NULL   ; NULL                
REMARK   3    UTILITY ANGLES            : NULL   ; NULL   ; NULL                
REMARK   3    UTILITY TORSION           : NULL   ; NULL   ; NULL                
REMARK   3    IDEAL-DIST CONTACT TERM   : 2350   ; 4.000  ; SEMIHARMONIC        
REMARK   3                                                                      
REMARK   3   RMS DEVIATIONS FROM IDEAL VALUES.                                  
REMARK   3    BOND LENGTHS                       (A) : 0.010                    
REMARK   3    BOND ANGLES                  (DEGREES) : 1.25                     
REMARK   3    PEPTIDE OMEGA TORSION ANGLES (DEGREES) : 2.42                     
REMARK   3    OTHER TORSION ANGLES         (DEGREES) : 23.20                    
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 2                                          
REMARK   3                                                                      
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: { A|* }                                                
REMARK   3    ORIGIN FOR THE GROUP (A):   29.8879   51.8147   19.4028           
REMARK   3    T TENSOR                                                          
REMARK   3     T11:    0.3164 T22:   -0.0019                                    
REMARK   3     T33:   -0.3105 T12:    0.0335                                    
REMARK   3     T13:   -0.0213 T23:    0.1824                                    
REMARK   3    L TENSOR                                                          
REMARK   3     L11:    4.9878 L22:    2.4162                                    
REMARK   3     L33:    5.4415 L12:    0.7208                                    
REMARK   3     L13:   -1.2630 L23:    0.3449                                    
REMARK   3    S TENSOR                                                          
REMARK   3     S11:    0.3901 S12:   -0.1353 S13:    0.8515                     
REMARK   3     S21:    0.1263 S22:   -0.0905 S23:   -0.1965                     
REMARK   3     S31:   -1.3013 S32:    0.4953 S33:   -0.2996                     
REMARK   3                                                                      
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: { B|* }                                                
REMARK   3    ORIGIN FOR THE GROUP (A):   45.1791   24.7621   26.2893           
REMARK   3    T TENSOR                                                          
REMARK   3     T11:   -0.0671 T22:    0.2896                                    
REMARK   3     T33:   -0.2637 T12:    0.2990                                    
REMARK   3     T13:   -0.2777 T23:    0.0826                                    
REMARK   3    L TENSOR                                                          
REMARK   3     L11:    2.7076 L22:    5.4371                                    
REMARK   3     L33:    1.3918 L12:    0.5523                                    
REMARK   3     L13:   -0.8163 L23:    1.1219                                    
REMARK   3    S TENSOR                                                          
REMARK   3     S11:    0.0775 S12:    0.0268 S13:   -0.7326                     
REMARK   3     S21:   -0.2518 S22:   -0.1644 S23:   -0.5158                     
REMARK   3     S31:    0.4814 S32:    1.3432 S33:    0.0869                     
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 6PRK COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 11-JUL-19.                  
REMARK 100 THE DEPOSITION ID IS D_1000242939.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 15-DEC-16                          
REMARK 200  TEMPERATURE           (KELVIN) : 93                                 
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SSRL                               
REMARK 200  BEAMLINE                       : BL9-2                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.88557                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : PIXEL                              
REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M                 
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-3000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-3000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 6195                               
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.200                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 9.950                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : 0.08000                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 17.2400                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.20                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.26                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 6PRH                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 64.25                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.44                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 18% (VOL/VOL) PEG500 MME, 8% (VOL/VOL)   
REMARK 280  MPD, 100 MM SODIUM NITRATE, 100 MM MOPS, SODIUM HEPES PH 7.3,       
REMARK 280  VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 62                             
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+2/3                                            
REMARK 290       3555   -X+Y,-X,Z+1/3                                           
REMARK 290       4555   -X,-Y,Z                                                 
REMARK 290       5555   Y,-X+Y,Z+2/3                                            
REMARK 290       6555   X-Y,X,Z+1/3                                             
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       58.42667            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       29.21333            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000       58.42667            
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       29.21333            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 8610 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 29070 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -67.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000       43.04500            
REMARK 350   BIOMT2   2  0.000000 -1.000000  0.000000       74.55613            
REMARK 350   BIOMT3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     TYR A     2                                                      
REMARK 465     ALA A     3                                                      
REMARK 465     THR A     4                                                      
REMARK 465     MET A     5                                                      
REMARK 465     VAL A   121                                                      
REMARK 465     GLY B    -1                                                      
REMARK 465     PRO B     0                                                      
REMARK 465     MET B     1                                                      
REMARK 465     THR B     2                                                      
REMARK 465     SER B   120                                                      
REMARK 465     THR B   121                                                      
REMARK 465     GLY B   122                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     SER A  12    OG                                                  
REMARK 470     LYS A 120    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    GLN A  23       48.87   -109.03                                   
REMARK 500    VAL A 115      -42.51   -153.39                                   
REMARK 500    GLU B  22       42.85    -85.92                                   
REMARK 500    ASP B  40       46.65    -94.22                                   
REMARK 500    LYS B  41      -37.10   -135.57                                   
REMARK 500    GLU B  63       85.16    -46.94                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  6PRK A    1   121  UNP    O34412   YLBF_BACSU       1    121             
DBREF  6PRK B    1   122  UNP    O31779   YMCA_BACSU       1    122             
SEQADV 6PRK GLY B   -1  UNP  O31779              EXPRESSION TAG                 
SEQADV 6PRK PRO B    0  UNP  O31779              EXPRESSION TAG                 
SEQRES   1 A  121  MET TYR ALA THR MET GLU SER VAL ARG LEU GLN SER GLU          
SEQRES   2 A  121  ALA GLN GLN LEU ALA GLU MET ILE LEU GLN SER GLU THR          
SEQRES   3 A  121  ALA GLU ASN TYR ARG ASN CYS TYR LYS ARG LEU GLN GLU          
SEQRES   4 A  121  ASP GLU GLU ALA GLY ARG ILE ILE ARG SER PHE ILE LYS          
SEQRES   5 A  121  ILE LYS GLU GLN TYR GLU ASP VAL GLN ARG PHE GLY LYS          
SEQRES   6 A  121  TYR HIS PRO ASP TYR ARG GLU ILE SER ARG LYS MET ARG          
SEQRES   7 A  121  GLU ILE LYS ARG GLU LEU ASP LEU ASN ASP LYS VAL ALA          
SEQRES   8 A  121  ASP PHE LYS ARG ALA GLU ASN GLU LEU GLN SER ILE LEU          
SEQRES   9 A  121  ASP GLU VAL SER VAL GLU ILE GLY THR ALA VAL SER GLU          
SEQRES  10 A  121  HIS VAL LYS VAL                                              
SEQRES   1 B  124  GLY PRO MET THR LEU TYR SER LYS LYS ASP ILE VAL GLN          
SEQRES   2 B  124  GLN ALA ARG ASN LEU ALA LYS MET ILE SER GLU THR GLU          
SEQRES   3 B  124  GLU VAL ASP PHE PHE LYS ARG ALA GLU ALA GLN ILE ASN          
SEQRES   4 B  124  GLU ASN ASP LYS VAL SER THR ILE VAL ASN GLN ILE LYS          
SEQRES   5 B  124  ALA LEU GLN LYS GLN ALA VAL ASN LEU LYS HIS TYR GLU          
SEQRES   6 B  124  LYS HIS GLU ALA LEU LYS GLN VAL GLU ALA LYS ILE ASP          
SEQRES   7 B  124  ALA LEU GLN GLU GLU LEU GLU GLU ILE PRO VAL ILE GLN          
SEQRES   8 B  124  GLU PHE ARG ASP SER GLN MET GLU VAL ASN ASP LEU LEU          
SEQRES   9 B  124  GLN LEU VAL ALA HIS THR ILE SER ASN GLN VAL THR ASN          
SEQRES  10 B  124  GLU ILE ILE THR SER THR GLY                                  
HELIX    1 AA1 SER A    7  GLN A   23  1                                  17    
HELIX    2 AA2 SER A   24  ASP A   40  1                                  17    
HELIX    3 AA3 GLU A   41  GLY A   64  1                                  24    
HELIX    4 AA4 ASP A   69  ASN A   87  1                                  19    
HELIX    5 AA5 ASN A   87  GLU A  117  1                                  31    
HELIX    6 AA6 SER B    5  GLU B   22  1                                  18    
HELIX    7 AA7 THR B   23  ASN B   39  1                                  17    
HELIX    8 AA8 LYS B   41  TYR B   62  1                                  22    
HELIX    9 AA9 LYS B   64  GLU B   83  1                                  20    
HELIX   10 AB1 ILE B   85  ILE B  117  1                                  33    
CRYST1   86.090   86.090   87.640  90.00  90.00 120.00 P 62          6          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.011616  0.006706  0.000000        0.00000                         
SCALE2      0.000000  0.013413  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.011410        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system