#=GF ID BD-FAE
#=GF AC PF20434.2
#=GF DE BD-FAE
#=GF AU Hameleers L;0000-0002-8132-3500
#=GF AU Faure R;0000-0002-5107-9009
#=GF AU Terrapon N;0000-0002-3693-6017
#=GF AU Hakulinen N;0000-0003-4471-7188
#=GF AU Deuss P;0000-0002-2254-2500
#=GF AU Master E;0000-0002-6837-9817
#=GF AU Jurak E;0000-0003-4253-4556
#=GF AU Mistry J;0000-0003-2479-5322
#=GF AU Chuguransky S;0000-0002-0520-0736
#=GF SE Hameleers Lisanne
#=GF GA 26.80 26.80;
#=GF TC 26.80 26.80;
#=GF NC 26.70 26.70;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch --cpu 4 -Z 75585367 -E 1000 HMM pfamseq
#=GF TP Family
#=GF CL CL0028
#=GF RN [1]
#=GF RM 34059129
#=GF RT Polysaccharide utilization loci-driven enzyme discovery reveals
#=GF RT BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active
#=GF RT on complex natural xylans.
#=GF RA Hameleers L, Penttinen L, Ikonen M, Jaillot L, Faure R, Terrapon
#=GF RA N, Deuss PJ, Hakulinen N, Master ER, Jurak E;
#=GF RL Biotechnol Biofuels. 2021;14:127.
#=GF DR SO; 0100021; polypeptide_conserved_region;
#=GF CC This family represents a novel bifunctional feruloyl and acetyl
#=GF CC xylan esterase (BD-FAE, previously known as bifunctional
#=GF CC carbohydrate esterase (CE)), which is active on complex natural
#=GF CC xylans and was identified as the basis of a monophyletic clade
#=GF CC gathering all homologs identified in PULs (polysaccharide
#=GF CC utilisation loci) predicted to act on xylan. It adopts an
#=GF CC alpha-beta-hydrolase fold with the catalytic triad Ser-Asp-His
#=GF CC [1]. This new family of proteins is a new candidate for biomass
#=GF CC processing due to its capacity to remove ferulic acid and acetic
#=GF CC acid from natural corn and birchwood xylan substrates [1].
#=GF SQ 15243
#=GS A0A4R5TV17_9MICC/32-231 AC A0A4R5TV17.1
#=GS A0A852V5I4_9ACTN/100-299 AC A0A852V5I4.1
#=GS A0A428XR68_9ACTN/157-362 AC A0A428XR68.1
#=GS A0A8H6ZBZ0_9AGAR/21-183 AC A0A8H6ZBZ0.1
#=GS A0A840Y9F7_9SPHN/88-287 AC A0A840Y9F7.1
#=GS A0A4Y8RJL9_9HYPH/53-243 AC A0A4Y8RJL9.1
#=GS C4FDZ9_9BIFI/79-283 AC C4FDZ9.1
#=GS A0A553FPR9_9BACT/60-186 AC A0A553FPR9.1
#=GS D1W0U4_9BACT/47-232 AC D1W0U4.1
#=GS A0A290QIK6_9BACT/38-241 AC A0A290QIK6.1
#=GS A0A1B8B021_FUSPO/73-271 AC A0A1B8B021.1
#=GS A0A0E0E663_9ORYZ/216-346 AC A0A0E0E663.1
#=GS A0A1G9B0T3_9MICO/88-287 AC A0A1G9B0T3.1
#=GS A0A1Q4HHH2_9MYCO/156-361 AC A0A1Q4HHH2.1
#=GS A0A402BE90_9CHLR/30-224 AC A0A402BE90.1
#=GS A0A374DUV0_9FIRM/31-219 AC A0A374DUV0.1
#=GS Q65FG3_BACLD/45-256 AC Q65FG3.1
#=GS A0A0F4R1S0_9GAMM/42-255 AC A0A0F4R1S0.1
#=GS A0A1C6NBP5_9NEIS/64-196 AC A0A1C6NBP5.1
#=GS A0A4R5DJL3_9BACT/48-237 AC A0A4R5DJL3.1
#=GS A0A0M2WMS7_9BURK/56-253 AC A0A0M2WMS7.1
#=GS A0A0P1BNM9_9BASI/64-291 AC A0A0P1BNM9.1
#=GS A0A368KQH7_9BACT/48-247 AC A0A368KQH7.1
#=GS A0A554VDB8_9FLAO/60-261 AC A0A554VDB8.1
#=GS A0A1Q3GQK6_9BACT/76-284 AC A0A1Q3GQK6.1
#=GS A0A2Z4LT24_9FLAO/45-192 AC A0A2Z4LT24.1
#=GS A0A7H5ERS3_9BACT/58-219 AC A0A7H5ERS3.1
#=GS A0A1W2F2J8_9SPHI/45-244 AC A0A1W2F2J8.1
#=GS IMCL2_ORYSJ/126-330 AC Q5VNW5.1
#=GS F0SDY7_PSESL/49-172 AC F0SDY7.1
#=GS A0A4R4ZI61_9ACTN/115-330 AC A0A4R4ZI61.1
#=GS Q1GC17_LACDA/23-217 AC Q1GC17.1
#=GS A0A840FCD4_9SPHN/73-271 AC A0A840FCD4.1
#=GS A0A127F154_9HYPH/90-198 AC A0A127F154.1
#=GS A0A377KJX6_9ENTE/22-134 AC A0A377KJX6.1
#=GS A0A565CM59_9BRAS/100-308 AC A0A565CM59.1
#=GS A0A1M5YQJ0_9DELT/80-194 AC A0A1M5YQJ0.1
#=GS A0A518G2Y6_9BACT/77-285 AC A0A518G2Y6.1
#=GS A0A1H9CNQ1_9FIRM/35-231 AC A0A1H9CNQ1.1
#=GS A0A8C4L6A3_EQUAS/75-263 AC A0A8C4L6A3.1
#=GS A0A518ATP4_9BACT/59-254 AC A0A518ATP4.1
#=GS A0A419SYT5_9FIRM/33-226 AC A0A419SYT5.1
#=GS A0A517ZP81_9PLAN/580-805 AC A0A517ZP81.1
#=GS A0A7K6IVI8_9CORV/56-240 AC A0A7K6IVI8.1
#=GS A0A1I3GLH9_9PLAN/50-248 AC A0A1I3GLH9.1
#=GS A0A543GH07_9PSEU/44-255 AC A0A543GH07.1
#=GS A0A518B657_9BACT/55-255 AC A0A518B657.1
#=GS A0A6N6NM68_9ACTN/36-250 AC A0A6N6NM68.1
#=GS A0A1D9P5Y4_9FIRM/22-222 AC A0A1D9P5Y4.1
#=GS A0A4V2EMT4_METCM/19-251 AC A0A4V2EMT4.1
#=GS A0A6I5WCM2_9ACTN/3-173 AC A0A6I5WCM2.1
#=GS A0A8J3QSD1_9ACTN/63-179 AC A0A8J3QSD1.1
#=GS R6V0G9_9FIRM/24-219 AC R6V0G9.1
#=GS A0A0Q4X8I8_9HYPH/56-273 AC A0A0Q4X8I8.1
#=GS A0A2U2JAC7_9FLAO/33-257 AC A0A2U2JAC7.1
#=GS A0A4R7BTR0_9HYPH/71-178 AC A0A4R7BTR0.1
#=GS A0A3S9ADH2_9BACL/23-152 AC A0A3S9ADH2.1
#=GS A0A1W2BDQ8_9SPHI/55-251 AC A0A1W2BDQ8.1
#=GS A0A1Z4K6G3_9CYAN/57-259 AC A0A1Z4K6G3.1
#=GS A0A6C2YRW8_9BACT/51-254 AC A0A6C2YRW8.1
#=GS W7YSI9_9BACT/59-261 AC W7YSI9.1
#=GS A0A4U1BZ22_9SPHI/38-236 AC A0A4U1BZ22.1
#=GS A0A1H4FGH2_9BACT/47-246 AC A0A1H4FGH2.1
#=GS A0A671TNC4_SPAAU/65-198 AC A0A671TNC4.1
#=GS A0A5A7WXY6_9MYCO/136-342 AC A0A5A7WXY6.1
#=GS A0A3N4B1Y7_YERPE/73-289 AC A0A3N4B1Y7.1
#=GS A0A0K2YLS4_9NOCA/17-209 AC A0A0K2YLS4.1
#=GS A0A1P8YCA4_9NOCA/91-299 AC A0A1P8YCA4.1
#=GS A0A1X1R0A2_MYCBE/141-347 AC A0A1X1R0A2.1
#=GS A0A846RZB3_9MICC/51-246 AC A0A846RZB3.1
#=GS E5CDZ8_9BACE/38-231 AC E5CDZ8.1
#=GS A0A1I1GGK8_9BACT/60-259 AC A0A1I1GGK8.1
#=GS A0A7W7QQY6_9ACTN/136-335 AC A0A7W7QQY6.1
#=GS A0A2K4ZFZ0_9FIRM/68-290 AC A0A2K4ZFZ0.1
#=GS D8UAI0_VOLCA/51-260 AC D8UAI0.1
#=GS A0A5N5JXP4_9PEZI/65-191 AC A0A5N5JXP4.1
#=GS A0A6M2BNV1_9GAMM/55-246 AC A0A6M2BNV1.1
#=GS F2K0X3_MARM1/43-255 AC F2K0X3.1
#=GS A0A2S6NLH2_RHOGL/64-162 AC A0A2S6NLH2.1
#=GS A0A8H6CYB7_9HYPO/21-162 AC A0A8H6CYB7.1
#=GS Q04D63_OENOB/17-221 AC Q04D63.1
#=GS A0A413I294_9FIRM/38-241 AC A0A413I294.1
#=GS A0A2T0XV65_9MICO/126-337 AC A0A2T0XV65.1
#=GS A0A0P0CN97_9BACT/78-274 AC A0A0P0CN97.1
#=GS F2JJD3_CELLD/25-222 AC F2JJD3.1
#=GS A0A346R0I6_9HYPH/51-162 AC A0A346R0I6.1
#=GS A0A174SJP7_9FIRM/54-265 AC A0A174SJP7.1
#=GS A0A5A8CJD3_CAFRO/57-210 AC A0A5A8CJD3.1
#=GS A0A2R7KYZ6_9SPHI/38-237 AC A0A2R7KYZ6.1
#=GS A0A179H7N2_PURLI/25-246 AC A0A179H7N2.1
#=GS A0A067G370_CITSI/191-321 AC A0A067G370.1
#=GS E5CB28_9BACE/100-286 AC E5CB28.2
#=GS A0A2G5ILB6_9ACTN/45-255 AC A0A2G5ILB6.1
#=GS A0A5E3WX68_9AGAM/21-141 AC A0A5E3WX68.1
#=GS A0A3S9MUR9_9FLAO/58-255 AC A0A3S9MUR9.1
#=GS A0A7T1KRG9_9CAUD/28-216 AC A0A7T1KRG9.1
#=GS A0A518DPW2_9BACT/87-237 AC A0A518DPW2.1
#=GS A0A426CZE0_9BACT/44-255 AC A0A426CZE0.1
#=GS A0A074KS71_9BACT/40-194 AC A0A074KS71.1
#=GS A0A098M625_9BACL/27-217 AC A0A098M625.1
#=GS A0A179DHA3_9SPHI/63-260 AC A0A179DHA3.1
#=GS A0A506UG26_9HYPH/57-237 AC A0A506UG26.1
#=GS A0A1Y1T4S1_9FLAO/52-250 AC A0A1Y1T4S1.1
#=GS A0A7W9M1E9_9PSEU/7-190 AC A0A7W9M1E9.1
#=GS A0A1X7C0R8_9ACTN/74-192 AC A0A1X7C0R8.1
#=GS A0A143PLV3_LUTPR/37-251 AC A0A143PLV3.1
#=GS A0A2W1Z6T1_9MICO/20-206 AC A0A2W1Z6T1.1
#=GS A0A519I953_9GAMM/61-186 AC A0A519I953.1
#=GS Q8A8H5_BACTN/37-222 AC Q8A8H5.1
#=GS A0A100W2T9_9MYCO/68-269 AC A0A100W2T9.1
#=GS A0A192D8Z6_9SPHN/28-167 AC A0A192D8Z6.1
#=GS A0A3M8G9J5_9FLAO/72-144 AC A0A3M8G9J5.1
#=GS A0A1W4XFU9_AGRPL/62-246 AC A0A1W4XFU9.1
#=GS A0A1H9MLB4_9BACT/62-199 AC A0A1H9MLB4.1
#=GS A0A1X9LQP2_9MICO/86-294 AC A0A1X9LQP2.1
#=GS I3TUL7_TISMK/66-196 AC I3TUL7.1
#=GS A0A269WAG5_9BACL/32-218 AC A0A269WAG5.1
#=GS A0A517T602_9PLAN/108-312 AC A0A517T602.1
#=GS A0A4T0UNV7_9ACTN/115-303 AC A0A4T0UNV7.1
#=GS A0A1Z5TN03_HORWE/51-260 AC A0A1Z5TN03.1
#=GS A0A6G6WCV3_9ACTN/153-359 AC A0A6G6WCV3.1
#=GS A0A4P7JQ34_9GAMM/50-186 AC A0A4P7JQ34.1
#=GS A0A4R8DFC6_9BACT/772-885 AC A0A4R8DFC6.1
#=GS A0A1Q9TZ25_9ACTN/109-316 AC A0A1Q9TZ25.1
#=GS K9HK94_9PROT/73-180 AC K9HK94.1
#=GS A0A167MC49_PHYB8/122-289 AC A0A167MC49.1
#=GS A0A2W7NLL4_9BACT/63-262 AC A0A2W7NLL4.1
#=GS F0M6A7_PSEPM/18-226 AC F0M6A7.1
#=GS A0A8H8BST0_9HELO/55-226 AC A0A8H8BST0.1
#=GS A0A4Y1WWU7_9BACT/45-215 AC A0A4Y1WWU7.1
#=GS A0A514WEF1_9SPHN/41-237 AC A0A514WEF1.1
#=GS A0A8H6FUZ4_9LECA/16-146 AC A0A8H6FUZ4.1
#=GS A0A4R0NRV1_9SPHI/43-213 AC A0A4R0NRV1.1
#=GS D8PWX9_SCHCM/21-229 AC D8PWX9.1
#=GS A0A7C9TM38_9BURK/109-252 AC A0A7C9TM38.1
#=GS A0A430BEY6_9SPHN/50-267 AC A0A430BEY6.1
#=GS M2YES7_9MICC/117-316 AC M2YES7.1
#=GS A0A286GIT2_9PROT/58-243 AC A0A286GIT2.1
#=GS A0A2G7GA65_9EURO/19-155 AC A0A2G7GA65.1
#=GS A5Z832_9FIRM/24-215 AC A5Z832.1
#=GS A0A4Q2M527_9BACL/34-228 AC A0A4Q2M527.1
#=GS A0A0N0LXP2_9SPHN/94-291 AC A0A0N0LXP2.1
#=GS Q8G7R1_BIFLO/51-273 AC Q8G7R1.1
#=GS A0A090W6B8_9FLAO/31-168 AC A0A090W6B8.1
#=GS T2KN86_FORAG/51-249 AC T2KN86.1
#=GS A0A517Y1J5_9BACT/572-686 AC A0A517Y1J5.1
#=GS A0A1R4F0S0_9MICO/18-214 AC A0A1R4F0S0.1
#=GS W0V3J4_9BURK/55-243 AC W0V3J4.1
#=GS A0A1L6JDL3_9SPHN/43-180 AC A0A1L6JDL3.1
#=GS A0A7Y5U042_9BACT/147-342 AC A0A7Y5U042.1
#=GS A0A1G6X9V7_9NOCA/17-210 AC A0A1G6X9V7.1
#=GS A0A517N185_9BACT/48-186 AC A0A517N185.1
#=GS A0A2K2G791_9SPHN/61-247 AC A0A2K2G791.1
#=GS A0A553WCL7_9SPHN/85-284 AC A0A553WCL7.1
#=GS A0A7J5TW73_9BACT/51-272 AC A0A7J5TW73.1
#=GS R5V8W0_9BACE/53-235 AC R5V8W0.1
#=GS A0A0Q4R7T3_9BACL/26-148 AC A0A0Q4R7T3.1
#=GS A0A6F9Z912_9BACT/37-222 AC A0A6F9Z912.1
#=GS A0A7W8HA40_9FIRM/104-297 AC A0A7W8HA40.1
#=GS A0A5C2H6Q2_9RHOB/63-245 AC A0A5C2H6Q2.1
#=GS A0A5N6DRW1_ASPPA/24-143 AC A0A5N6DRW1.1
#=GS A0A396RYU6_9SPHN/62-261 AC A0A396RYU6.1
#=GS A0A8H3HUM8_9LECA/2298-2468 AC A0A8H3HUM8.1
#=GS A0A545TYP3_9PROT/61-247 AC A0A545TYP3.1
#=GS A0A6P1MHA0_9BACT/49-176 AC A0A6P1MHA0.1
#=GS A0A4R2FLP5_9GAMM/77-270 AC A0A4R2FLP5.1
#=GS A0A8H6I0Z6_9AGAR/55-236 AC A0A8H6I0Z6.1
#=GS A0A7I8DFN9_9FIRM/29-222 AC A0A7I8DFN9.1
#=GS A0A1C5GTY1_9ACTN/30-217 AC A0A1C5GTY1.1
#=GS A0A512M8C0_9BACT/557-773 AC A0A512M8C0.1
#=GS A0A1C5BSV7_9ACTN/53-270 AC A0A1C5BSV7.1
#=GS A0A5A8CPX5_CAFRO/115-259 AC A0A5A8CPX5.1
#=GS A0A4P6JJM8_9CHLR/22-232 AC A0A4P6JJM8.1
#=GS A0A7L2TU24_POMRU/56-240 AC A0A7L2TU24.1
#=GS A4CN49_ROBBH/32-239 AC A4CN49.1
#=GS A0A1H0P302_9ACTN/129-281 AC A0A1H0P302.1
#=GS A0A4S3K191_9GAMM/31-228 AC A0A4S3K191.1
#=GS R5PA10_9BACT/47-232 AC R5PA10.1
#=GS A0A558RB81_9SPHN/135-211 AC A0A558RB81.1
#=GS A0A1I3R3Q7_9HYPH/50-235 AC A0A1I3R3Q7.1
#=GS A0A5C6DB74_9BACT/40-180 AC A0A5C6DB74.1
#=GS A0A202E726_9EURY/70-282 AC A0A202E726.1
#=GS A0A419S702_9SPHI/78-285 AC A0A419S702.1
#=GS A0A2T6AJM4_9RHOB/62-166 AC A0A2T6AJM4.1
#=GS A0A7R8YVP2_HERIL/61-214 AC A0A7R8YVP2.1
#=GS A0A0P4R4P1_9ACTN/32-222 AC A0A0P4R4P1.1
#=GS A0A813E3Y8_POLGL/139-345 AC A0A813E3Y8.1
#=GS E8QY85_ISOPI/64-269 AC E8QY85.1
#=GS A0A1D8ATM3_9BACT/68-265 AC A0A1D8ATM3.1
#=GS U4JUT2_9VIBR/208-434 AC U4JUT2.1
#=GS A0A257M3H5_9BACT/83-204 AC A0A257M3H5.1
#=GS A0A1D9PCG7_9FLAO/84-284 AC A0A1D9PCG7.1
#=GS A0A1C5JMG5_9ACTN/138-346 AC A0A1C5JMG5.1
#=GS A0A164Q7V3_9AGAM/52-161 AC A0A164Q7V3.1
#=GS A0A7W8DIG8_9BACT/43-235 AC A0A7W8DIG8.1
#=GS A0A812R543_9DINO/871-1081 AC A0A812R543.1
#=GS A0A210Q8U8_MIZYE/71-195 AC A0A210Q8U8.1
#=GS A0A417XWW6_9ACTN/17-213 AC A0A417XWW6.1
#=GS A1ZIB2_9BACT/66-274 AC A1ZIB2.1
#=GS A0A7W7WYM8_9PSEU/16-186 AC A0A7W7WYM8.1
#=GS A0A174MK77_9CLOT/17-216 AC A0A174MK77.1
#=GS A0A0Q8CFD7_9MICO/77-281 AC A0A0Q8CFD7.1
#=GS A4BZL2_9FLAO/59-236 AC A4BZL2.1
#=GS A0A810L010_9ACTN/151-281 AC A0A810L010.1
#=GS A0A0D0BZG8_9AGAR/58-229 AC A0A0D0BZG8.1
#=GS A0A2V0P214_9CHLO/105-300 AC A0A2V0P214.1
#=GS A0A5C5Z405_9BACT/68-273 AC A0A5C5Z405.1
#=GS A0A2S0WHV4_9ACTN/42-231 AC A0A2S0WHV4.1
#=GS A0A4S3KH38_9GAMM/72-197 AC A0A4S3KH38.1
#=GS B9T5X2_RICCO/144-353 AC B9T5X2.1
#=GS A0A0C3DHP9_9AGAM/52-186 AC A0A0C3DHP9.1
#=GS A0A223S6A3_9ACTN/18-205 AC A0A223S6A3.1
#=GS A0A371SMK8_9BACI/25-218 AC A0A371SMK8.1
#=GS A0A318BFL4_9CAUL/57-190 AC A0A318BFL4.1
#=GS A0A507B6B2_9PEZI/55-172 AC A0A507B6B2.1
#=GS A0A3A4ARI0_9ACTN/29-227 AC A0A3A4ARI0.1
#=GS A0A0Q6W5L0_9BURK/71-183 AC A0A0Q6W5L0.1
#=GS D4X6N3_9BURK/63-168 AC D4X6N3.1
#=GS A0A1Y2MXU0_PSEAH/70-265 AC A0A1Y2MXU0.1
#=GS A0A1B9GYE5_9TREE/32-199 AC A0A1B9GYE5.1
#=GS A0A4R4RYV1_9ACTN/23-172 AC A0A4R4RYV1.1
#=GS A0A1H7G7R7_9BACT/61-256 AC A0A1H7G7R7.1
#=GS A0A173W6E1_9ACTN/28-247 AC A0A173W6E1.1
#=GS A0A5S5DLW2_9SPHI/44-225 AC A0A5S5DLW2.1
#=GS A0A316G2I2_9RHOB/103-221 AC A0A316G2I2.1
#=GS A0A654E6T6_9BACT/65-233 AC A0A654E6T6.1
#=GS A0A4R1DFH4_9ACTN/33-145 AC A0A4R1DFH4.1
#=GS A0A4S5EB18_9MICC/16-214 AC A0A4S5EB18.1
#=GS A0A5J5KVU4_9MICC/86-289 AC A0A5J5KVU4.1
#=GS A0A839LAN6_9BURK/56-176 AC A0A839LAN6.1
#=GS A0A813RKJ9_9BILA/55-115 AC A0A813RKJ9.1
#=GS A0A654E0I7_9BACT/48-216 AC A0A654E0I7.1
#=GS A0A543C012_9ACTN/46-169 AC A0A543C012.1
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